Positive Metagenomic Next-Generation Sequencing of Renal Lavage Fluid Associates with Delayed Graft Function in Kidney Transplants from Donors After Circulatory Death: A Retrospective Study.

Background: Delayed graft function (DGF) is a major complication in kidney transplants from donation after circulatory death (DCD). This study assessed the association between metagenomic next-generation sequencing (mNGS) results and the occurrence of DGF during the perioperative period in DCD kidney transplant recipients.

Methods: We analyzed 191 DCD kidney transplant recipients in this single-center retrospective cohort study.

Immune-mediated membranous nephropathy: Innovations in pathogenetic modeling and mechanistic insights.

Membranous nephropathy (MN), an autoimmune cause of adult nephrotic syndrome, is driven by podocyte-targeting antibodies against PLA2R/THSD7A. Current models fail to fully capture human disease progression. This review evaluates three transformative approaches: (1) Heterologous antibody-induced models enabling acute injury replication; (2) Antigen-driven immunization modeling adaptive immunity; and (3) GBF-on-Chip platforms mimicking filtration barrier dynamics.

Direct Carboboration of Aryl Alkenes with Stable Organoborons Through Ziegler-Type Addition.

Carbometallation reaction represents a classic strategy for the bifunctionalization of unsaturated hydrocarbons, yet conventional implementations predominantly rely on either highly reactive organometallic reagents or transition metal catalysts. Notably, the direct employment of metalloid organoboron reagents for such addition processes remains elusive except when specific substrate combinations are employed. Herein, a base-catalyzed carboboration reaction of aryl alkenes with bench-stable benzylic boronates is reported.

The role of the mitochondrial dynamic distribution in oocyte development arrest induced by F-53B.

Chlorinated polyfluoroalkyl ether sulfonates (F-53B), an alternative to perfluorooctane sulfonate (PFOS), has gained attention for reproductive toxicity, but data on its specific effects on oocyte remain limited. We conducted in vitro maturation (IVM) experiments on ICR mouse oocytes exposed to F-53B at its medium inhibition of maturation concentration (IM). PFOS and 0.

Thrombopoietin Mitigates Neuronal Death by Enhancing Mitophagy and Suppressing NLRP3 Inflammasome Activation Under Oxygen-Glucose Deprivation Conditions.

Thrombopoietin (TPO), a principal hematopoietic cytokine, regulates the development and proliferation of megakaryocytes and platelets. Our previous research demonstrated TPO's neuroprotective role against hypoxic-ischemic brain injury in rats. Yet, the underlying mechanisms remain unclear.

Discovery of thiacyanine dyes as a new class of potent coronavirus inhibitors that suppress viral RNA synthesis.

Topoisomerase poisons are clinically used anticancer drugs that can induce DNA cleavage complexes to block replication. TOP3B is the only topoisomerase that can catalyze topological changes on either DNA or RNA and induce cleavage complexes on both nucleic acids. We proposed that TOP3B poisons may inhibit coronavirus RNA genome replication and tested this hypothesis by using mouse hepatitis coronavirus (MHV).

Targeting Mitochondrial Quality Control for the Treatment of Triple-Negative Breast Cancer: From Molecular Mechanisms to Precision Therapy.

Breast cancer is the leading threat to the health of women, with a rising global incidence linked to social and psychological factors. Among its subtypes, triple-negative breast cancer (TNBC), which lacks , , and expression, is highly heterogeneous with early metastasis and a poor prognosis, making it the most challenging subtype. Mounting evidence shows that the mitochondrial quality control (MQC) system is vital for maintaining cellular homeostasis.

Antibody-oligonucleotide conjugates in cancer therapy: Potential and Promise.

X-Drug Conjugates (XDCs) achieve precise therapy through a modular design comprising three key components: targeting vehicle, linker, and therapeutic payload. Among these, Antibody-Drug Conjugate (ADCs) have demonstrated the most remarkable progress in clinical research. By leveraging their precise targeting mechanism and robust clinical data, ADCs have emerged as frontrunner therapeutics in oncology.

Oral contrast-enhanced ultrasonographic features and radiomics analysis to predict NIH risk stratification for gastrointestinal stromal tumors.

Objective: To evaluate the value of oral contrast-enhanced ultrasonography and radiomics analysis in predicting the National Institutes of Health (NIH) staging of gastrointestinal stromal tumors (GISTs).

Methods: A retrospective cohort study was conducted on 204 patients presenting with GISTs in Tianjin Medical University Cancer Institute and Hospital from January 2020 to January 2023. The clinical profiles, oral contrast-enhanced ultrasonography (CEUS), and endoscopic ultrasound (EUS) imaging data were collected.

Gut microbiota dysbiosis exacerbates heart failure by the LPS-TLR4/NF-κB signalling axis: mechanistic insights and therapeutic potential of TLR4 inhibition.

Objective: This study aimed to investigate the associations between gut microbiota dysbiosis and alterations in cardiac function and to elucidate the underlying molecular mechanisms involved.

Methods: Eighteen rats were divided into a control group (n = 6), a heart failure (HF) group (n = 6), and a TAK-242 intervention group (n = 6). Cardiac function was assessed using small-animal echocardiography.

Current challenges and emerging opportunities of chimeric antigen receptor-engineered cell immunotherapy.

Chimeric antigen receptor (CAR) engineered cellular immunotherapy offers the potential for precise targeting and elimination of tumor cells, providing a tailored approach to cancer treatment. CAR-T cells demonstrate significant anti-tumor activity among these therapies. Nonetheless, these therapies may trigger adverse effects, including inflammatory and neurotoxic reactions during treatment.

Organometallic-type reactivity of stable organoboronates for selective (hetero)arene C-H/C-halogen borylation and beyond.

Organometallic reagents are essential tools in both academic and industrial laboratories and the polarity separation within the carbon-metal bonds endows them with exceptional reactivities, but also imposes limitations, including air- and moisture-sensitivity, and flammability. Here, we demonstrate that stable and easily accessible benzylic (or allylic) boronate with alkali-metal alkoxide as the activator can act as reactive organometallic reagents. This strategy enables transition metal-free deprotonative C-H borylation of diverse (hetero)arenes.

A buoyant plasmonic microbubble-based SERS sensing platform for amyloid-beta protein detection in Alzheimer's disease.

Amyloid-β (Aβ) plaques are a key pathological hallmark of Alzheimer's disease (AD), highlighting the need for highly sensitive bioassays for Aβ detection to enable AD diagnosis. Here, we synthesized a buoyant plasmonic substrate composed of polyvinyl alcohol microbubbles (MBs) decorated with -reduced gold nanoparticles (Au NPs). Benefiting from its inherent buoyancy and near-infrared plasmonic properties, the Au/MB substrate serves as an ideal platform for biomolecular sensing the surface-enhanced Raman spectroscopy (SERS) technique.

Air Pollution and Stroke: An Emerging Challenge From Cardio-Cerebrovascular Multimorbidity.

Cerebrovascular disease, as represented by stroke, was identified as one of the leading causes of death worldwide. Although air pollution was widely documented to be associated with stroke, the underlying mechanism of such adverse effects remained largely unknown. Cardiovascular effects triggered by air pollution were regarded as the dominant contributor to stroke pathogenesis, indicating the interconnected pathological basis of cardio-cerebrovascular multimorbidity in driving acute cerebrovascular events.

Distribution, Sources, and Health Risk of Short-, Medium- and Long-Chain Chlorinated Paraffins in School-Area Ambient PM: A Study from the Pearl River Delta, China.

Background: Only a few studies have reported on chlorinated paraffin (CP) levels, especially long-chain chlorinated paraffins (LCCPs), in submicron particulate matter (PM) in the outdoor air around primary and secondary schools.

Methods: This study examined concentrations of short-chain CPs (SCCPs), medium-chain CPs (MCCPs), and LCCPs in PM samples from 96 schools across six cities in China's Pearl River Delta region during the winter (October to December 2018).

Results: The median total CP concentration was 34 ng/m, with median values for SCCP, MCCP, and LCCP of 17.

A Tyndall Effect-Based Visible Aptasensing Platform Enables Sensitive and Isolation-Free Profiling of Small Extracellular Vesicle Surface Proteins.

Rapid and portable profiling of surface proteins on small extracellular vesicles (sEV) is crucial for noninvasive cancer screening but remains technically challenging. Here, we present a Tyndall effect (TE)-based visible aptasensing platform (TEVAP) for direct, low-cost, and isolation-free detection of sEV surface proteins from complex biological samples. Aptamer-conjugated gold nanoparticles specifically bind to sEV, forming large-scale composites that enhance the TE signal.

Cross-feeding and co-degradation within a bacterial consortium dominated by challenging-to-culture sp. HA-1 enhances sulfonamide degradation.

The use of sulfonamides (SAs) leads to residual environmental pollution. Bacteria play a crucial role in the degradation of SAs, and microbial consortium offers advantages over single bacterium. However, the complexity of the degradation processes and interaction mechanisms within such consortia remains a mystery.

Clinical prediction of prognostic outcome of intravenous thrombolysis in mild stroke.

Development and validation of an easy-to-visualize nomogram for acute mild ischemic stroke (MIS) with unfavorable outcome 3 months after application of alteplase intravenous thrombolysis. A retrospective cohort study analysis was conducted at the Baoding First Central Hospital, involving 461 patients diagnosed with acute MIS who received alteplase thrombolysis within the treatment time window. The LASSO regression technique was employed to identify significant variables and develop nomograms.

Glycoluril Polymers for Codelivery in Solid Tumor Therapy.

Multicomponent chemotherapy has increasingly become a strategy of great importance in clinical nonsmall cell lung cancer treatments. However, this type of combination chemotherapy has not led to synergistic drug activities. Herein, we present the codelivery of doxorubicin and oxaliplatin via supramolecular nanocapsules.

Dietary saturated fatty acids and prostate cancer: insights into NF-κB pathway and lipid metabolism mechanisms.

Prostate cancer (PCa) is one of the most prevalent malignancies in men, with dietary factors, particularly saturated fatty acids (SFAs), emerging as significant contributors to its pathogenesis. This review synthesizes current literature linking dietary fat intake, especially from dairy products, to increased prostate cancer risk, highlighting specific SFAs such as myristic and palmitic acids. The nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway is identified as a critical mediator in this relationship, promoting cell survival and proliferation in the tumor microenvironment.

Efficacy and safety of Y-3 intracalvariosseous injection versus intravenous injection in the treatment of acute large hemispheric infarction (SOLUTION-2): rationale and design of a multicentre, prospective, randomised, open-label, blind endpoint (PROBE) trial.

Rationale: The blood-brain barrier (BBB) largely restricts the efficacy of neuroprotectants, which is an important approach in treating large hemispheric infarction (LHI). In this study, we harnessed intracalvariosseous (ICO) injection to bypass the BBB and facilitate drug delivery.

Aim: To compare the efficacy and safety of ICO injection versus intravenous injection in the treatment of acute LHI within 24 hours of disease onset.

ADFound: A Foundation Model for Diagnosis and Prognosis of Alzheimer's Disease.

Alzheimer's disease (AD) is an incurable neurodegenerative disorder characterized by progressive cognitive and functional decline. Consequently, early diagnosis and accurate prediction of disease progression are of paramount importance and inherently complex, necessitating the integration of multi-modal data. However, most existing methods are task-specific models that lack generalization ability, addressing only one task at a time and failing to simultaneously assess disease diagnosis and progression.

Structure-informed design of an ultrabright RNA-activated fluorophore.

RNA-based fluorogenic aptamers, such as Mango, are uniquely powerful tools for imaging RNA that activate the fluorescence of a weakly or non-fluorescent small molecule when bound. A central challenge has been to develop brighter, more specific and high-affinity aptamer-ligand systems for cellular imaging. Here we report an ultrabright fluorophore for the Mango II system discovered using a structure-informed, fragment-based small-molecule microarray approach.