Clonal divergence and genomic meltdown in prostatic pleomorphic giant cell adenocarcinoma.

Background: Pleomorphic giant cell adenocarcinoma (PGCA) of the prostate is a rare, aggressive variant characterized by multinucleated giant cells, sarcomatoid features, and resistance to conventional therapies. Despite its recognition in the WHO 2016 guidelines, the molecular drivers and clinicopathological correlates of PGCA remain poorly characterized. This study presents the first integrative clinicogenomic profiling of PGCA, revealing a novel prognostic gene signature with direct implications for diagnosis and treatment.

Ionic Liquid Top Surface Engineering: In Situ GIWAXS Insights Into 1D/3D Heterojunction Perovskite Formation.

Low-dimensional perovskites have been demonstrated repeatedly to improve the performance of perovskite photovoltaic devices in both light-to-electricity conversion efficiency and device durability. In this work, the ionic liquid (IL) 1-ethyl-3-methylimidazolium hydrogen sulfate (EMIMHSO) is innovatively introduced as a capping layer, which interacts with the residual PbI on the 3D perovskite top surface to generate the 1D perovskite, EMIMPbI. By adjusting the concentration of the IL, 1D perovskite formations with distinct morphologies is achieved.

Structurally conserved human anti-A35 antibodies protect mice and macaques from mpox virus infection.

The A35 protein, expressed on the enveloped virion of monkeypox (mpox) virus (MPXV), is essential for viral infection and spread within the host, making it an effective antiviral target. In this study, we demonstrated two human anti-A35 monoclonal antibodies (mAbs) displayed potential protection against MPXV in CAST/EiJ mice and rhesus macaques. Using cryo-electron microscopy, we determined two high-resolution structures of the A35 dimer in complex with the fragment of antigen binding of mAb 975 or mAb 981, revealing detailed interactions at the antigen-antibody interfaces.

Enhancing fibroblast-based bone regeneration by harnessing nanosecond pulsed electric field.

Despite their terminally differentiated state, human dermal fibroblasts (HDFs) can undergo osteogenic differentiation in vivo under certain pathological conditions, making them promising candidates for bone tissue engineering-though replicating this in vitro would be difficult. Building on prior findings that low-intensity (5 kV/cm) nanosecond pulsed electric fields (nsPEF) can partially reprogram HDFs toward pluripotency and boost their osteogenic capacity, an in vivo bone regeneration complex was fabricated by encapsulating nsPEF-treated cells in a self-healing hydrogel composed of oxidized hyaluronic acid and hydroxypropyl chitosan. In nude mice, these HDFs produced more robust ectopic bone both subcutaneously and within cranial defects, with significantly higher histological scores than untreated controls.

The clinical characteristics and diagnostic and treatment protocol for 14 acute Vibrio vulnificus infections caused by aquatic products.

Vibrio vulnificus infections caused by aquatic product-related injuries pose severe clinical challenges due to their rapid progression and high morbidity and mortality. Early diagnosis and timely intervention are critical to improving patient outcomes, yet standardized diagnostic and treatment protocols remain limited. We conducted a retrospective descriptive case series of 14 patients with confirmed V vulnificus infection admitted between 2020 and 2023.

Early diagnosis of primary open-angle glaucoma using isolated-check visual evoked potential with blue-on-yellow perimetry.

Background: Early detection of primary open-angle glaucoma (POAG) is crucial, as current diagnostic methods often miss early-stage damage. Combining blue-on-yellow perimetry with isolated-check visual evoked potential (Ic-VEP) could enhance the sensitivity and specificity of early POAG detection.

Objective: To evaluate the diagnostic efficacy of Ic-VEP combined with blue-on-yellow perimetry for early POAG detection.

Achieving Long-Term Cyclability in Sodium-Ion Batteries: Site-Selective Doping to Inhibit Irreversible Phase Transitions in P2-NaNiMnO Cathode.

The typical P2-type NaNiMnO exhibits a high theoretical capacity for sodium-ion batteries (SIBs). However, its P2-O2 phase transition during deep charging causes severe structural degradation and capacity decay. In this work, we propose a site-selective doping strategy based on multielement synergy to suppress irreversible phase transitions.

Global Trends and Cross-country Inequalities of Acute Hepatitis E in the Elderly, 1990-2021: A Comprehensive Analysis.

Background And Aims: Acute hepatitis E (AHE) in the elderly can lead to severe complications including liver failure and mortality, yet the epidemiological landscape remains poorly characterized. This study aimed to assess the burden, trends, and health inequalities of AHE among the elderly over the past three decades, and to further predict its changes by 2030.

Methods: Data on AHE in the elderly were obtained from the Global Burden of Disease 2021.

The Role of Psychological Distress and Unhealthy Lifestyle in Angina with Nonobstructive Coronary Arteries: A Cross-Sectional Study.

Purpose: This study aimed to investigate the impact of mental health and lifestyle factors on women with angina with nonobstructive coronary arteries (ANOCA), and to identify potential risk factors associated with ANOCA.

Methods: In this cross-sectional study, 84 female patients diagnosed with ANOCA and 42 age-matched healthy women served as controls. All participants underwent endothelial function testing and laboratory assessments.

Effects of different neuromuscular training modalities on balance performance in older adults: a systematic review and network meta-analysis.

Background: Neuromuscular training (NMT) is widely utilized to enhance balance and reduce fall risk in older adults, yet comparative effectiveness across various modalities remains unclear. This study aimed to systematically assess and rank the effects of sensorimotor training (ST), whole-body vibration training (WBVT), neurofunctional training (NT), and balance training (BT) on balance performance in older adults.

Methods: A systematic review and network meta-analysis was conducted following PRISMA guidelines, including 49 randomized controlled trials with a total of 3,028 older adults.

Coexistence of CSF anti-Ma2 antibody and 14-3-3 protein: a diagnostic dilemma between autoimmune encephalitis and Creutzfeldt-Jakob disease, a Case Report.

Background: Anti-Ma2 antibody encephalitis is a rare paraneoplastic autoimmune encephalitis (AE) caused by anti-Ma2 antibody. Creutzfeldt-Jakob disease (CJD), a group of human prion diseases, is a rapidly advancing and fatal neurodegenerative disorder. The two diseases may display comparable clinical symptoms that are easily misdiagnosed.

Case Report: A rare presentation of vascular Ehlers-Danlos syndrome with a massive hemothorax and a chest wall hematoma.

Background: Vascular Ehlers-Danlos syndrome (vEDS) is a rare but life-threatening subtype of the Ehlers-Danlos syndromes (EDS), a group of inherited connective tissue disorders with significant clinical and genetic heterogeneity. vEDS is mainly caused by mutations in the gene, leading to type III collagen abnormalities. vEDS is characterized by increased vascular fragility and predisposition to serious complications such as arterial rupture and gastrointestinal perforation.

A method for efficient separation of polystyrene nanoplastics and its application in natural freshwater.

Nanoplastics (NPs) are an emerging contaminant in natural freshwater environments. However, there is a lack of analytical methods for separating and characterizing NPs by particle size, which is essential for analyzing their environmental behavior. Asymmetrical flow field-flow fractionation (AF4) is considered a reliable technique suitable for separating and characterizing the particle size of macromolecules, colloids, and particles.

Universal 3D-Printing of Suspended Metal Oxide Nanowire Arrays on MEMS for AI-Optimized Combinatorial Gas Fingerprinting.

Additive manufacturing technology has the potential to provide great versatility in the design and fabrication of sensing devices. This prerequisite necessitates further technological improvement in precision and material diversity. Here, a universal meniscus-guided 3D printing method is reported that can fabricate freestanding metal oxide semiconducting nanowires with programmed compositions and compatible substrate options at the single-entity level.

Oncometabolite 5-IP inhibits inositol 5-phosphatase to license E-cadherin endocytosis.

E-cadherin downregulation is an epithelial-mesenchymal transition hallmark canonically attributed to transcriptional repression. Here we delineate a metabolite-driven endocytic route of E-cadherin downregulation in inflammation-associated colorectal cancer (CRC). Specifically, IP kinase-2 (IP6K2), a 5-diphosphoinositol pentakisphosphate (5-IP) synthase upregulated in patients with CRC, is activated via a ROS-Src phosphorylation axis elicited by dextran sulfate sodium (DSS), generating 5-IP around adherens junction (AJ) to promote E-cadherin endocytosis and the transcriptional activities of β-catenin.

Coevolutionary phage training expands phage host range: Driven by tail fiber mutations in Pseudomonas aeruginosa lytic phage?

The rise of antibiotic-resistant bacteria, particularly multidrug-resistant Pseudomonas aeruginosa (P. aeruginosa), represents a critical challenge to global health security. Bacteriophages, with their ability to target and lyse specific bacterial pathogens, present a promising alternative through phage therapy.

MAFLD outperforms NAFLD in identifying metabolic dysfunction in U.S. adolescents: a NHANES 2017-2020 analysis.

Background: The newly proposed metabolic-associated fatty liver disease (MAFLD) definition better reflects the metabolic pathogenesis shared with non-alcoholic fatty liver disease (NAFLD), though with incomplete diagnostic overlap; this study systematically compares their prevalence and risk profiles in U.S. adolescents to inform optimal clinical management strategies.

Paclitaxel-based strategy as first-line therapy for advanced breast cancer: a model-based economic evaluation for China.

Aim: The limited number of cost-utility studies of breast cancer conducted in China makes comparisons among different paclitaxel-based strategies difficult. To provide such data, a cost-utility analysis comparing albumin-bound formulation (nab)-paclitaxel (ABPTX), paclitaxel polymer micelles (PTXPM), and paclitaxel (PTX) for advanced breast cancer was performed.

Methods: Tree Age Pro 2011 software was used to simulate a Markov model to perform a health economic evaluation.

Variable π-d orbital hybridization in 2D transition metal-organic frameworks.

Two-dimensional metal-organic frameworks (2D MOFs) have emerged as promising platforms for exploring novel quantum phenomena and tunable electronic functionalities. Here, we investigate π-d orbital hybridization in monolayer M(HAT) (M = Ni, Co, Fe; HAT = 1,4,5,8,9,12-hexaazatriphenylene) frameworks by combining density functional theory (DFT) calculations and scanning tunneling microscopy/spectroscopy (STM/STS) characterization. Despite identical lattice geometries, the Ni-HAT framework exhibits a dispersive, gapless band structure, while the Co- and Fe-HAT frameworks display localized electronic states and semiconducting bandgaps.

Brain-Inspired In-Memory Data Pruning and Computing with TaO Mem-Selectors.

The selective attention mechanisms inherent in the human visual system provide a promising framework for developing edge systems that can simultaneously prune and process critical information from visual input. However, conventional complementary metal-oxide-semiconductor-based edge vision systems rely on complex digital logic for data pruning, alongside the physical separation of pruning, memory, and processing. This increases both power consumption and latency.

Wastewater-based epidemiology of influenza A virus in Shenzhen: baseline values and implications for multi-pathogen surveillance.

Influenza A virus (IAV) has become increasingly unpredictable in its seasonal patterns following the COVID-19 pandemic. A key challenge remains identifying optimal moments for public health interventions to inform evidence-based decisions. Real-time PCR was employed to quantify IAV concentrations in wastewater samples from 38 treatment plants in Shenzhen, China (n = 2,764), collected weekly from March 2023 to March 2024.

Physician Use of Large Language Models: A Quantitative Study Based on Large-Scale Query-Level Data.

Background: Generative artificial intelligence (GenAI) has rapidly emerged as a promising tool in health care. Despite its growing adoption, how physicians make use of it in medical practice has not been qualitatively studied. Existing literature has largely focused on theoretical applications or experimental validations, with limited insight into real-world physician engagement with GenAI technologies.

HEP14-activated PKC-ERK1/2 pathway boosts HEP14-empowered hADSCs for ovarian regeneration and functional restoration.

Premature ovarian insufficiency (POI) and age-related natural-aging ovarian insufficiency (ARNA-OI) pose pressing global health challenges, necessitating effective therapeutic strategies and a deep understanding of their underlying mechanisms. This study investigates how HEP14, a PKC pathway activator, boosts the regenerative potential of human adipose-derived stem cells (hADSCs) for ovarian regeneration. Transcriptome analysis reveals that HEP14 modulates gene expression profile in hADSCs, enhancing their regenerative capacity.