Whole-Genome Sequencing Analysis of the Multidrug-Resistant Strain AC1520 Isolated from a Patient with Urinary Tract Infection.

The Gram-negative bacterium is an important opportunistic facultative anaerobic pathogen. In the present study, we aimed to elucidate the whole-genome sequence of the multidrug-resistant (MDR) strain AC1520, detailing its acquired antibiotic resistance genes (ARGs) and their genetic elements. The strain AC1520 was isolated from a urine sample taken from a patient with urinary tract infection.

Coexistence of CSF anti-Ma2 antibody and 14-3-3 protein: a diagnostic dilemma between autoimmune encephalitis and Creutzfeldt-Jakob disease, a Case Report.

Background: Anti-Ma2 antibody encephalitis is a rare paraneoplastic autoimmune encephalitis (AE) caused by anti-Ma2 antibody. Creutzfeldt-Jakob disease (CJD), a group of human prion diseases, is a rapidly advancing and fatal neurodegenerative disorder. The two diseases may display comparable clinical symptoms that are easily misdiagnosed.

CircNOX4 promotes proliferation and resistance by regulating the hsa-miR-6884-5p/YWHAG Axis and ERK signaling pathway in cholangiocarcinoma.

The biological function of circular RNAs (circRNAs) has been increasingly implicated in tumor drug resistance. However, their role in intrahepatic cholangiocarcinoma (ICC) remains poorly understood, and the underlying mechanisms are largely unexplored. In this study, we identified a key circRNA, circNOX4, through data mining and experimental validation in ICC.

Tertiary lymphoid structures-driven immune infiltration patterns and their association with survival in neuroblastoma.

Background: Neuroblastoma (NB), a diverse childhood cancer, needs better prognostic markers for personalized treatment. The current clinical risk stratification system does not fully explain the high heterogeneity of tumor patients. Tertiary lymphoid structures (TLS), key in tumor immunity, may serve as new biomarkers, but their impact on NB prognosis is unclear.

Overexpression of MEOX2 inhibits breast cancer cell metastasis by targeting oxidative stress-induced RGS5.

This study aimed to investigate the role of mesenchymal homeobox 2 (MEOX2) on breast cancer cell metastasis and its underlying mechanism. Overexpression of MEOX2 in human lymphatic endothelial cell (HLEC) lines was established to assess the adhesion and transendothelial migration of MCF7 and MDA-MB-231 cells to the HLEC cells. After being treated with the oxidative stress inducer HO and the antioxidant N-acetylcysteine (NAC), cell viability, reactive oxygen species (ROS) levels, adhesion, and transendothelial migration of MCF7 and MDA-MB-231 cells to HLEC cells were detected.

Association between body mass index and long-term all-cause mortality in critically ill patients without malignant tumors.

Background: The "obesity paradox" in certain diseases has been reported in previous studies. This study aimed to investigate the relationship between BMI and long-term mortality in all critically ill patients without malignant tumors who were admitted to the ICU.

Methods: Using the MIMIC-IV 2.

STING-ΔN, a novel splice isoform of STING, modulates innate immunity and autophagy in response to DNA virus infection.

Background: Stimulator of interferon (IFN) genes (STING) is a central adaptor protein in the cGAS-STING signaling pathway, orchestrating the production of type I interferons (IFNs) in response to cytosolic DNA detection, a crucial mechanism in antiviral defense. However, further investigation is needed to understand how post-transcriptional regulation, particularly alternative splicing, modulates STING activity.

Methods: We identified a novel alternatively spliced isoform of STING, termed STING-∆N, resulting from exon 3 skipping.

Research on equipment fault diagnosis model based on gan and inverse PINN: Solutions for data imbalance and rare faults.

In the field of medical imaging equipment, fault diagnosis plays a vital role in guaranteeing stable operation and prolonging service life. Traditional diagnostic approaches, though, are confronted with issues like intricate fault modes, as well as scarce and imbalanced data. This paper puts forward a fault diagnosis model integrating digital twin technology and Inverse Physics - Informed Neural Networks (Inverse PINN).

Case Report: Two cases of chemotherapy refractory aggressive variant prostate cancer with extreme durable response to PARP inhibitor.

Background: Aggressive variant prostate cancer (AVPC) represents a distinct clinical subset characterized by resistance to novel hormone therapies and an unfavorable prognosis, frequently associated with the concurrent loss of tumor suppressor genes (TSG) such as PTEN, RB1, and TP53. While the progression-free survival (PFS) and overall survival (OS) of AVPC are relatively short, the optimal first-line treatment remains unclear.

Presentation: In this case report, we presented two AVPC cases who have ultimately benefited from the usage of PARP inhibitors.

STING-∆C, a novel splice isoform of STING, inhibits DNA virus-induced innate immunity and autophagy.

Stimulator of interferon genes (STING) plays a critical role in the innate immune response to cytosolic DNA, primarily activating type I interferons (IFNs). Although alternative splicing is known to modulate immune pathways, the influence of STING splice isoforms requires further exploration. Here, we identified STING-∆C, a novel splice isoform of STING generated by retention of intron 6, resulting in a truncated C-terminus.

Organic light-emitting transistors with high efficiency and narrow emission originating from intrinsic multiple-order microcavities.

Narrow electroluminescence is in high demand for high-resolution displays, optical communication and medical phototherapy. Organic light-emitting transistors, as three-terminal electroluminescent devices, offer advantages in simplifying device architecture and achieving high efficiency under gate regulation. However, achieving high efficiency and narrow emission remains a challenge.

IDR-driven TOLLIP condensates antagonize the innate antiviral immunity by promoting the deSUMOylation of MAVS.

Mitochondrial antiviral signaling protein (MAVS) is a central adaptor protein in retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) signaling against RNA viral infection. Posttranslational modifications (PTMs) play a critical role in modulating the activity of MAVS. However, how phase separation regulates the PTMs to fine-tune MAVS activation remains to be elucidated.

Fluoxetine-induced downregulation of circMap2k1 signaling cascade to improve neurological function after ischemic stroke.

Background: Ischemic stroke (IS) is known for its high incidence, disability, and mortality, and there is an urgent need to investigate the pathophysiological mechanisms and develop novel treatment strategies.

Objectives: We aimed to investigate the mechanisms of the novel circMap2k1/miR-135b-5p/Pidd1 axis in the treatment of IS progression with fluoxetine.

Methods: The middle cerebral artery occlusion (MCAO) model was done in adult male Sprague-Dawley (SD) rats and followed by fluoxetine treatment and the injection of adeno-associated virus (AAV)-sh-ctr and AAV-sh-circMap2k1 into the bilateral hippocampal tissues of rats.

Data-driven models for microfluidics: A short review.

Microfluidic devices have many unique practical applications across a wide range of fields, making it important to develop accurate models of these devices, and many different models have been developed. Existing modeling methods mainly include mechanism derivation and semi-empirical correlations, but both are not universally applicable. In order to achieve a more accurate and general modeling process, the use of data-driven modeling has been studied recently.

Identification of clinical risk factors for coronary artery lesions in children with Kawasaki disease: a retrospective cohort study.

Background: Coronary artery lesions are the most severe complications of Kawasaki disease. Despite recent advances, evidence of the association between risk factors and coronary artery lesion is lacking. In this study, we demonstrated the potential clinical indicators that could assist to evaluate the prevalence of coronary artery lesion among paediatric patients with Kawasaki disease.

Micro-Scale Ice Shoveling Effect Induced by Magnetic-Responsive Microfins.

Icing is ubiquitous in nature and engineering applications, and imposes threats to road and air transportations, wind energy infrastructures, etc. However, current active de-icing solutions, especially the most popular one, i.e.

Conserved moonlighting protein pyruvate dehydrogenase induces robust protection against infection.

Developing an effective () vaccine has been a challenging endeavor, as demonstrated by numerous failed clinical trials over the years. In this study, we formulated a vaccine containing a highly conserved moonlighting protein, the pyruvate dehydrogenase complex E2 subunit (PDHC), and showed that it induced strong protective immunity against epidemiologically relevant staphylococcal strains in various murine disease models. While antibody responses contributed to bacterial control, they were not essential for protective immunity in the bloodstream infection model.

Investigating the impact of STING pathway activation on breast cancer treatment outcomes: development and validation of a prognostic model.

Introduction: Breast cancer (BRCA) is a significant cause of cancer-associated mortality across the globe. Current therapeutic approaches face challenges such as drug resistance and metastasis. Immune signaling is triggered by chromosomal instability (CIN) generates misplaced DNA structures that activate the cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) pathway, triggering.

Effect of Raloxifene Treatment on Apolipoproteins and Lipoprotein(a) Concentrations in Postmenopausal Women: A Meta-Analysis of Randomized Controlled Trials.

Background And Aim: Although various randomized controlled trials (RCTs) have evaluated the effect of raloxifene on apolipoproteins and lipoprotein(a) concentrations in postmenopausal women, the results have been inconsistent and inconclusive. Therefore, we conducted this meta-analysis of RCTs to investigate the effect of raloxifene administration on apolipoproteins and lipoprotein(a) [Lp(a)] concentrations in postmenopausal women.

Methods: Two independent researchers systematically searched the scientific literature (including PubMed/Medline, Scopus, Web of Science, and EMBASE) for English-language randomized controlled trials (RCTs) published up to June 2024.

Hypoxia-induced LAMB2-enriched extracellular vesicles promote peritoneal metastasis in gastric cancer via the ROCK1-CAV1-Rab11 axis.

Peritoneal metastasis is one of the most common risk factors contributing to the poor prognosis of gastric cancer. We previously reported that extracellular vesicles from gastric cancer cells could facilitate peritoneal metastasis. However, their impact on gastric cancer-induced peritoneal metastasis under hypoxic conditions remains unclear.

Multi-Objective Optimization of Laser Cleaning Quality of Q390 Steel Rust Layer Based on Response Surface Methodology and NSGA-II Algorithm.

To improve the laser cleaning surface quality of rust layers in Q390 steel, a method of determining the optimal cleaning parameters is proposed that is based on response surface methodology and the second-generation non-dominated sorting genetic algorithm (NSGA-II). It involves constructing a mathematical model of the input variables (laser power, cleaning speed, scanning speed, and repetition frequency) and the objective values (surface oxygen content, rust layer removal rate, and surface roughness). The effects of the laser cleaning process parameters on the cleaning surface quality were analyzed in our study, and accordingly, NSGA-II was used to determine the optimal process parameters.

Diagnostic, predictive and prognostic molecular biomarkers in clear cell renal cell carcinoma: A retrospective study.

Clear cell renal cell carcinoma (ccRCC) is a common and aggressive subtype of kidney cancer. Many patients are diagnosed at advanced stages, making early detection crucial. Unfortunately, there are currently no noninvasive tests for ccRCC, emphasizing the need for new biomarkers.

Na[AlBPO(OH)](HO) and Na[AlBPO](HO): Two Remarkable Complex Aluminum Borophosphates.

Two remarkable aluminum borophosphates (AlBPOs), namely, Na[AlBPO(OH)](HO) (denoted as ABPO1) and Na[AlBPO](HO) (denoted as ABPO2), have been designed and prepared by low-temperature flux syntheses. The exceptional open framework structure of ABPO1 is formed by a unique microanionic network [AlBPO(OH)], which contains three types of 8-, 12-, and 16-membered ring (MR) tunnels. Interestingly, these tunnels are featured by a type of super-nanocage as large as ∼1.

A Stochastic Model Based on Optimal Satellite Subset Selection Strategy for Smartphone Pseudorange Relative Positioning.

In order to overcome the limitations of traditional stochastic models for smartphones, we introduce a double-difference code pseudorange residual (DDPR)-dependent stochastic model based on an optimal satellite subset, with the goal of mitigating the constraints imposed by the quality of GNSS observations in smartphones on the accuracy and reliability of phone-based GNSS positioning. In our methodology, the satellite selection process involved considering the integrated carrier-to-noise density ratio (C/N0) index of both the reference station and the smartphone, enabling us to construct a satellite subset characterized by superior observation quality. Furthermore, by leveraging the optimal subset of satellites and incorporating the C/N0-dependent stochastic model, we could determine the approximate location of the terminal through pseudorange differential positioning.