Global trends of antimicrobial resistance and virulence of Klebsiella pneumoniae from different host sources.

Background: Klebsiella pneumoniae is ubiquitous in animals, humans, and the environment, facilitating the dissemination of antimicrobial resistance (AMR) and virulence traits. Most studies are primarily focused on human clinical isolates, leaving critical gaps in understanding non-human reservoirs and cross-species transmission risks.

Methods: We combined large-scale genomic analyses with in vitro and in vivo infection models to characterize the evolutionary dynamics of 2809 K.

Cold Inducible RNA-Binding Protein: A Novel Therapeutic Target for Ventricular Arrhythmia Control.

Ventricular arrhythmias following myocardial infarction (MI) remain a leading cause of sudden cardiac death, yet therapeutic options are limited by incomplete understanding of the molecular mechanisms governing post-infarction arrhythmogenesis. While RNA-binding proteins have emerged as critical regulators of cardiovascular pathophysiology, their role in cardiac electrophysiology remains largely unexplored. Here, we demonstrate that cold- inducible RNA-binding protein (CIRP) functions as a critical regulator of ventricular arrhythmia susceptibility through post-transcriptional control of cardiac ion channels.

Pediatric metabolic dysfunction-associated steatotic liver disease and the gut microbiome: from research landscape to targeted modulation.

Metabolic dysfunction-associated steatotic liver disease (MASLD), formally known as non-alcoholic fatty liver disease, has become the most common form of chronic liver disease in children. The spectrum of pediatric MASLD ranges from simple steatosis to steatohepatitis, fibrosis, cirrhosis, and in rare cases, hepatocellular carcinoma. Its pathogenesis involves a complex interplay among genetic, epigenetic, and environmental factors, along with alterations in the gut microbiota and its associated metabolites.

Nonpharmacological interventions for childbirth-related post-traumatic stress disorder and accompanying symptoms: A network meta-analysis of randomized controlled trials.

Background: Childbirth-related post-traumatic stress disorder (CB-PTSD) often coexists with depression and anxiety, but there's a lack of tailored, comparative non-pharmacological treatments.

Objective: This study investigated the efficacy of non-pharmacological interventions in the prevention and alleviation of symptoms associated with CB-PTSD and its accompanying symptoms.

Methods: A comprehensive literature search was conducted across multiple databases, including PubMed, the Cochrane Library, Web of Science, Embase, China National Knowledge Infrastructure, China Science and Technology Journal Database, Wanfang, and China Biology Medicine, up to September 2024.

An artificial intelligence model to predict mortality among hemodialysis patients: A retrospective validated cohort study.

Hemodialysis stands as the most prevalent renal replacement therapy globally. Accurately identifying mortality among hemodialysis patients is paramount importance, as it enables the formulation of tailored interventions and facilitates timely management. The objective of the study was to establish and validate an artificial intelligence (AI) model to predict mortality among hemodialysis patients.

Ghrelin/GHSR-1a promotes angiogenesis after myocardial infarction through the glycolytic process.

Background: Therapeutic angiogenesis has demonstrated efficacy in revascularizing ischemic heart tissue and reducing the progression of cardiac remodeling following myocardial infarction. Recent studies have highlighted the significance of the glycolytic process in maintaining endothelial cell function and cardiac homeostasis. However, the specific role of glycolysis in angiogenesis post-myocardial infarction remains poorly understood.

A quadruplex real-time fluorescent quantitative PCR detection method for identification and serotyping of Pasteurella multocida.

Pasteurella multocida is a zoonotic pathogen with the ability to infect a diverse range of hosts, including humans through contact with pets. Due to its diverse capsular serogroups, rapid and accurate identification, as well as serotyping, are essential for the effective prevention and control of infections caused by this bacterium. This study developed a quadruplex quantitative PCR (qPCR) method for the identification and serotyping of P.

Isolation, Passage, and Pathogenicity of a Newly Isolated Strain From Hubei, China.

is the causative agent of porcine proliferative enteropathy (PPE)-a disease of great economic impact to the global pig industry, but the isolation and continuous passage culture of this bacterial species is very difficult which limits the development of inactivated or live vaccines. While China is the largest pig rearing country in the world, only one study has reported the isolation of . .

Shared genetic loci connect cardiovascular disease with blood pressure and lipid traits in East Asian populations.

Introduction: Cardiovascular diseases (CVDs), including myocardial infarction (MI), heart failure (HF), atrial fibrillation (AF), and arrhythmia, are major contributors to global mortality and often share overlapping risk factors and pathophysiological mechanisms. While genome-wide association studies (GWAS) have identified many loci for individual CVDs, the shared genetic architecture across related traits-particularly in East Asian populations-remains underexplored.

Materials And Methods: We integrated large-scale GWAS summary statistics from East Asian populations to perform genome-wide and local genetic correlation analyses across four CVD phenotypes and five cardiometabolic traits (blood pressure and lipid levels).

Prednisone-aided gross thyroid remnant ablation using 5.55 GBq of radioiodine in patients with differentiated thyroid cancer.

Background: Radioiodine ( 131 I) remnant ablation (RRA) with activities ranging from 1.1-3.7 GBq showed dismal efficacy but a dose-effect relationship in patients with differentiated thyroid cancer (DTC) after less-than-total thyroidectomy.

Neuroendocrine circuit for sleep-dependent growth hormone release.

Sleep is known to promote tissue growth and regulate metabolism, partly by enhancing growth hormone (GH) release, but the underlying circuit mechanism is unknown. We demonstrate how GH release, which is enhanced during both rapid eye movement (REM) and non-REM (NREM) sleep, is regulated by sleep-wake-dependent activity of distinct hypothalamic neurons expressing GH-releasing hormone (GHRH) and somatostatin (SST). SST neurons in the arcuate nucleus suppress GH release by inhibiting nearby GHRH neurons that stimulate GH release, whereas periventricular SST neurons inhibit GH release by projecting to the median eminence.

Two new species and additional records of the genus Shavrin, 2024 from Xizang, southwest China (Coleoptera, Staphylinidae, Omaliinae).

Yang & Peng, (Xizang: Langkazi) and Yang & Peng, (Xizang: Cuona) are described and illustrated. Additional records of (Cheng & Peng, 2020) are reported. A key to the Chinese species of the genus Shavrin, 2024 is given.

Effects of essential oils on calf growth, ruminal fermentation, and antioxidative status: a meta-analysis.

Introduction: Essential oils (EO) have gained significant attention in the calves industry due to their antimicrobial properties. This meta-analysis aimed to evaluate the efficacy of EO on calves to provide better guidance for cattle feed.

Methods: We conducted a comprehensive search of relevant studies published from inception to February 6, 2022, using PubMed, CENTRAL,Web of Science, and EMBASE.

Genomic characteristics and pathogenicity of three newly isolated NADC34-like PRRSV strains in China and evaluation of one strain for inactivated vaccine candidate.

Porcine reproductive and respiratory syndrome (PRRS) has caused substantial economic losses to the pig industry. Recently, the emergence of NADC34-like PRRS virus (PRRSV) has made the worldwide circulation of PRRSV more complex. In 2017, the first detection of NADC34-like PRRSV in China was reported, but the pathogenicity and biological characteristics of this novel PRRSV variant remain to be further elucidated.

Machine tool FEM model correction assisted by dynamic evolution sequence.

In the simulation analysis of large-scale industrial instruments such as machine tools, in order to ensure simulation accuracy, model parameter correction is necessary. This research presents a machine tool model correction method assisted by the dynamic evolution sequence (DES). The method first introduces a dynamic evolution method to generate a uniformly distributed sequence, replacing the traditional sequence used in Kriging surrogate models, and constructing a more accurate Kriging surrogate model for machine tools.

Deep learning predicts HER2 status in invasive breast cancer from multimodal ultrasound and MRI.

The preoperative human epidermal growth factor receptor type 2 (HER2) status of breast cancer is typically determined by pathological examination of a core needle biopsy, which influences the efficacy of neoadjuvant chemotherapy (NAC). However, the highly heterogeneous nature of breast cancer and the limitations of needle aspiration biopsy increase the instability of pathological evaluation. The aim of this study was to predict HER2 status in preoperative breast cancer using deep learning (DL) models based on ultrasound (US) and magnetic resonance imaging (MRI).

Development and application of a triplex real-time PCR method for the detection of , , and .

(LI), (Bhy), and (Cp) are three common swine enteric pathogens widespread worldwide, leading to huge economic losses to the pig industry. LI, Bhy, and Cp are co-infected and should not be distinguished, and the detection of bacterial nucleic acids in feces allows rapid identification of whether a herd is infected with these three pathogens. In this study, we developed and optimized a TaqMan triple quantitative real-time PCR (qPCR) method for LI, Bhy, and Cp, which was validated and applied to the analysis of actual samples from pigs.

Meningeal malignant solitary fibrous tumor with multiple recurrence, extracranial extension, cervical lymph node metastases: case report and review of the literature.

Introduction: Solitary fibrous tumor (SFT) of the central nervous system (CNS) is a spindle cell neoplasm originating from mesenchymal tissue. SFT is prone to local recurrence or distant metastasis. The main sites of metastasis include the liver, lung, and bone.

Identification of a broad-spectrum lytic Bordetella phage and assessments of its potential for combating Bordetella infections.

Bordetella bronchiseptica (Bb) is a zoonotic respiratory pathogen that frequently causes infections in farming and companion animals, posing threats to agricultural economics and public health. However, Bb strains are intrinsically resistant to several antibiotics commonly used to treat respiratory infections. Phage therapy has been recognized as a promising strategy to combat bacterial infections.

Multi-trait GWAS identifies pleiotropic loci associated with colorectal cancer in East Asian populations.

Introduction: While genome-wide association studies (GWAS) have identified numerous susceptibility loci in Colorectal cancer (CRC), most findings are based on European populations. Additionally, CRC shares genetic architecture with other traits, and multi-trait analysis can improve the discovery of pleiotropic loci.

Materials And Methods: We conducted a multi-trait GWAS using the Multi-Trait Analysis of GWAS (MTAG) framework, leveraging large-scale genomic and phenotypic data from BioBank Japan (BBJ).

Advanced lung cancer inflammation index is associated with mortality in critically ill patients with non-traumatic cerebral hemorrhage.

Existing prognostic tools for non-traumatic intracerebral hemorrhage (NCH) mainly focus on neurological injury severity but overlook systemic factors that cause secondary deterioration. This study aimed to evaluate the Advanced Lung Cancer Inflammation Index (ALI), a biomarker combining nutritional parameters (BMI, albumin) and inflammatory parameters (neutrophil-to-lymphocyte ratio, NLR), as a new prognostic indicator for NCH. It aimed to fill the gap in quantifying the synergy between inflammation and malnutrition in neurocritical care.

Isolation, Antimicrobial Susceptibility, and Genotypes of Three Species Prevalent on Pig Farms in China Between 2021 and 2023.

(PM), (GPS), and (APP) are among the species with the top five isolation rates on Chinese pig farms annually. To understand the antimicrobial susceptibility and genotypes of these three pathogens that are currently prevalent on pig farms, we investigated 151 bacterial strains (64 PM, 48 GPS, and 39 APP) isolated from 4190 samples from farms in 12 Chinese provinces between 2021 and 2023. The prevalent serotypes were PM type D (50.

ATF3 Within the Interferon Signaling Pathway: A Potential Biomarker for Predicting Pathological Response to Neoadjuvant Chemoimmunotherapy.

Background: Neoadjuvant chemoimmunotherapy has achieved high downstaging and pathologic response rates in nonsmall-cell lung cancer (NSCLC), but outcomes vary significantly. Early identification of beneficiaries remains a challenge.

Methods: This study analyzed baseline transcriptomic data from 24 NSCLC patients (9 major pathological response [MPR], 15 nonmajor pathological response [NMPR]) treated with neoadjuvant chemoimmunotherapy, sourced from the GEO database.

[Optical coherence tomography angiography and microvessel density quantification in penumbra after traumatic brain injury in rats].

Objective: To observe the dynamic changes of microvascular injury and repair in the penumbra of traumatic brain injury (TBI) rats with effective cerebral perfusion microvascular imaging using optical coherence tomography angiography (OCTA).

Methods: Transparent closed cranial windows were placed in craniotomy rats after TBI caused by weight drop. All the rats in TBI group and control group underwent head MRI examination on the first postoperative day, and the changes of cerebral cortical microvessel density were measured by OCTA through cranial windows on d0, d2, d4, d6, and d8.