A novel dual-ligand luminescent Eu-based metal-organic framework for ratiometric fluorescence sensing and visual detection of tetracycline.

Tetracycline (TC) residues in animal-derived food pose a significant threat to human health, necessitating the development of straightforward and user-friendly methods for TC detection to safeguard food safety. Here, a series of novel Eu-based metal-organic frameworks (Eu-MOFs) were prepared using a dual-ligand strategy. In the midst of these Eu-MOFs, Eu-ABPDC/BPDC exhibits the dual emission at 472 and 619 nm under single-wavelength excitation, enabling highly effective ratiometric fluorescence sensing of TC.

RNF126 suppresses amino acid-mediated mTORC1 signaling pathway by ubiquitinating ILF3 in HEK293T cells.

The mammalian Target of Rapamycin Complex 1 (mTORC1) serves as a metabolic hub that integrates external nutrients to promote cell growth and metabolism, with its activation closely associated with accelerated cancer progression. Interleukin enhancer-binding factor 3 (ILF3) has been identified as a negative regulator of mTORC1 by tethering GATOR1/2 to the lysosomal membrane during amino acid sensing. However, the regulatory mechanisms of the ILF3-mediated mTORC1 signaling pathway remain unclear.

Photochromic Hydrophobic Eutectogel with High Adhesion, Stability, and Self-Healing for Information Encryption, Underwater Wireless Communications, and Motion Monitoring.

As an outstanding flexible conductive material, eutectogels have received increasing attention due to their potential applications in fields such as flexible wearable electronics and wireless communications. However, the development of multifunctional eutectogels with stability in underwater environments is still very challenging due to the high sensitivity of most deep eutectic solvents (DES) to water. Herein, a hydrophobic eutectogel is fabricated by simple photopolymerization, the introduction of macromolecular cross-linking agents, fluorine-rich monomers and dynamic light-responsive functional ammonium molybdate tetrahydrate (Mo), as well as multiple dynamic interactions within the eutectogel, endowed it with excellent mechanical properties (160 kPa), long-term underwater repetitive adhesion (102.

Assessing Canine Parvovirus Vaccine Performance in Puppies with Maternally Derived Antibody: An Improved Study Design.

: Typically, studies aiming to assess the ability of canine parvovirus (CPV) vaccines to immunise puppies with maternally derived antibody (MDA) are undertaken using group-housed puppies. Since live attenuated vaccine virus is invariably shed in the faeces, this can result in repeated oral re-exposure and puppies which failed to respond to the initial vaccination may respond instead to shed vaccine virus in the environment, thus artificially enhancing the efficacy of the vaccine. This problem can be avoided by adopting a pair-housed study design where one vaccinated pup is housed with one unvaccinated sentinel.

On the reversibility of RNA deamination versus RNA methylation: exploring the proximate and ultimate causes.

RNA modifications play a crucial role in regulating gene expression, splicing, decoding, translation, and degradation. Among the most studied modifications are adenosine-to-inosine (A-to-I) RNA editing and N-methyladenosine (mA). While mA is reversible, enabling dynamic regulation of gene expression; A-to-I editing is irreversible, leading to permanent changes in RNA sequences.

participates in stress tolerance and contributes to bacterial pathogenicity.

The gene in bacteria, which plays a key role in adaptation to various environmental stresses, is associated with pathogenicity. However, its role in conferring external environmental stress resistance in remains unclear. In this study, we used the clinically isolated serotype 5 CF7066 strain (wild type) to investigate the effects of on the external environmental stress response of by constructing mutant () and complemented strains.

Strong Coupling of NiOx and Self-Assembled Molecules via Inserted Reductant for High-Performance Inverted Perovskite Solar Cells.

Self-assembled molecules (SAMs) deposited on nickel oxide (NiO) are the basis for achieving high-performance inverted perovskite solar cells (PSCs). Unfortunately, the dissolution and redeposition of SAMs caused by the perovskite precursors leads to leaky monolayers, resulting in perovskite degradation and reduced stability. Here, a novel method is reported to realize strong coupling between NiO and SAMs via inserted reductant [9tris(2-carboxyethyl)phosphine hydrochloride (TCEP)] for an integrated NiO-SAMs hole transport layer (HTL).

Protocol for mapping 2'-O-methylation using nanopore direct RNA-seq data with NanoNm.

2'-O-methylation (Nm) is pivotal in rRNA and the internal site of mRNA. Here, we present an integrated bioinformatics protocol for the identification of 2'-O-methylation using nanopore direct RNA sequencing (RNA-seq) data. We describe steps for software installation, data collection, and training the machine learning model.

Macrophage Migration Inhibitory Factor Suppresses Natural Killer Cell Response and Promotes Hypoimmunogenic Stem Cell Engraftment Following Spinal Cord Injury.

Human induced pluripotent stem cells (iPSCs) offer immense potential as a source for cell therapy in spinal cord injury (SCI) and other diseases. The development of hypoimmunogenic, universal cells that could be transplanted to any recipient without requiring a matching donor could significantly enhance their therapeutic potential and accelerate clinical translation. To create off-the-shelf hypoimmunogenic cells, we used CRISPR-Cas9 to delete B2M (HLA class I) and CIITA (master regulator of HLA class II).

Iron-Immune Crosstalk at the Maternal-Fetal Interface: Emerging Mechanisms in the Pathogenesis of Preeclampsia.

Preeclampsia (PE) is a pregnancy-specific hypertensive disorder characterized by systemic inflammation, endothelial dysfunction, and placental insufficiency. While inadequate trophoblast invasion and impaired spiral artery remodeling have long been recognized as central to its pathogenesis, emerging evidence underscores the critical roles of dysregulated iron metabolism and its crosstalk with immune responses, particularly macrophage-mediated inflammation, in driving PE development. This review systematically explores the dynamic changes in iron metabolism during pregnancy, including increased maternal iron demand, placental iron transport mechanisms, and the molecular regulation of placental iron homeostasis.

Different mRNAs encoding identical proteins: how and why?

Alternative splicing (AS) produces various forms of mRNAs and protein isoforms and contributes to biodiversity. However, different mRNAs might have identical CDS and encode the same protein sequence. It is unclear why organisms need these distinct mRNAs if they encode the same protein? We propose two complementary hypotheses, namely adaptive hypothesis and error hypothesis, and tested these ideas using genomes of four representative organisms, human, mouse, fruitfly, and Arabidopsis.

Application and mechanism of percutaneous puncture disc platelet-rich plasma injection for lumbar disc herniation: a systematic review and meta-analysis.

Background: Although platelet-rich plasma (PRP) has been increasingly used for the treatment of lumbar disc herniation (LDH), its effect on patient outcomes lacks systematic assessment. This study aimed to compare the effects of PRP treatment and other treatments in LDH patients using the Oswestry Disability Index (ODI) and two visual analog scales (VASs) via a meta-analysis.

Methods: We systematically searched relevant studies from the PubMed, Embase, and Cochrane Library databases from their inception to October 24, 2024.

In Situ Synthesis of Ordered Macroporous Metal Oxides Monolayer on MEMS Chips: Toward Gas Sensor Arrays for Artificial Olfactory.

Metal oxide semiconductor gas sensors have attracted particular attention due to their merits of high sensitivity and easy integration. However, their insufficient selectivity severely limited their applications, especially in identifying low-concentration gases in a complex environment. Herein, inspired by the human olfaction, an in situ colloidal assembly strategy is developed to directly synthesize ordered macroporous metal oxide monolayers on micro-electromechanical system (MEMS) chips.

Gene Therapy vs Cochlear Implantation in Restoring Hearing Function and Speech Perception for Individuals With Congenital Deafness.

Importance: OTOF gene therapy (GT) has been shown to improve hearing and speech. The efficacy of GT remains to be compared against cochlear implantation (CI), the current gold standard for congenital deafness.

Objective: To evaluate treatment outcomes in auditory and speech perception between patients with congenital deafness treated with GT, CI, or both.

An EGFR Co-Amplified and De Novo Long Noncoding RNA HELDR Promotes Glioblastoma Malignancy through KAT7-Driven Gene Programs.

EGFR amplification frequently happens within extrachromosome DNAs (ecDNAs) and is a major mutation in glioblastoma (GBM). However, targeting EGFR for GBM treatments has been unsuccessful. Here we characterized a long non-coding RNA (lncRNA) that is co-amplified with EGFR within ecDNAs that we name hidden EGFR long non-coding downstream RNA (HELDR).

Endocrine traits during pregnancy in women with polycystic ovary syndrome - insights from a prospective cohort study in China.

Pregnant women with Polycystic ovary syndrome (PCOS) often experience exacerbated endocrine and metabolic dysfunction. While existing studies lack prospective data exist for Chinese populations. Our study aimed to characterize endocrine profiles in Chinese PCOS pregnancies using a prospective cohort.

GA/HbA1c Ratio Predicts the Occurrence of Diabetic Ketoacidosis and Disease Severity in Children with Type 1 Diabetes Mellitus.

Background: Pediatric type 1 diabetes mellitus (T1DM) is a significant endocrine condition, with diabetic ketoacidosis (DKA) posing a life-threatening risk. The aim of this study was to investigate the potential of the glycated albumin (GA) to glycosylated hemoglobin (HbA1c) ratio in predicting the occurrence of DKA and disease severity in children with T1DM.

Methods: In this study, 224 children with T1DM were retrospectively analyzed and divided into non-DKA group (n = 142) and DKA group (n = 82).

Macrophage Migration Inhibitory Factor Suppresses Natural Killer Cell Response and Promotes Hypoimmunogenic Stem Cell Engraftment Following Spinal Cord Injury.

Human induced pluripotent stem cells (iPSCs) offer immense potential as a source for cell therapy in spinal cord injury (SCI) and other diseases. The development of hypoimmunogenic, universal cells that could be transplanted to any recipient without requiring a matching donor, could significantly enhance their therapeutic potential and accelerate clinical translation. To create off-the-shelf hypoimmunogenic cells, we used CRISPR-Cas9 to delete B2M (HLA class I) and CIITA (master regulator of HLA class II).

Ketone drink enhances therapeutic efficacy in prostate cancer by targeting EZH2.

It is well established that EZH2, a lysine methyltransferase, is upregulated in most aggressive cancers, highlighting the importance of EZH2 in cancer progression. Recent research has shown that metabolic reprogramming is pivotal in various biological processes, including cancer. Despite this, evidence of EZH2's role in regulating cancer metabolism remains limited.

Targeting GPX4-Dependent Ferroptosis via a Dihydroartemisinin-Conjugated Cross-Linked Lipoic Acid Nanodrug for Endometrial Carcinoma Therapy.

Endometrial cancer (EC) is a significant global cause of cancer-related mortality. Chemoresistance is a major challenge in treating advanced or recurrent EC, necessitating the search for new anti-EC drugs. Dihydroartemisinin (DHA)-induced ferroptosis shows promise as a therapy for EC, but its poor solubility and insufficient antitumor potency hinder its clinical use.

Social Capital and Job Performance: A Moderated Mediation Model of Organizational Citizenship Behaviors and Psychological Capital.

Taiwan's high-tech industry is experiencing rapid labor restructuring driven by automation and technological advancement, resulting in increased job demands and workplace stress. In this context, identifying mechanisms that sustain employee performance has become a critical concern. Drawing on the concept of social capital and grounded in Conservation of Resources (COR) theory, this study investigates whether social capital indirectly enhances job performance through organizational citizenship behavior.

Multifunctional carboxymethyl cellulose-based gel fabric with fluorescent warning for fire rescue.

A multifunctional carboxymethyl cellulose-based gel fabric with fluorescent warning, motion sensing, and flame retardant protection was fabricated by crosslinking copolymerization and freeze-drying strategy. During the formation of multifunctional gel fabric, hydrogen-, coordination-, chemical- bonds, and abundant network structures were generated. These synergies give multifunctional fabrics reliable and durable flame retardant, fluorescent, and mechanical properties.

Colon Enzyme-Activated Prebiotic Nanomedicine for Targeted Therapy of Inflammatory Bowel Disease.

Inflammatory bowel disease is commonly treated with conventional therapies that suffer from poor drug bioavailability and significant side effects. This work develops a nanoparticle system using inulin, a naturally occurring polysaccharide, to address these challenges. This colon-targeted oral drug delivery system enables precise and efficient treatment of IBD by leveraging the colon-specific degradation of inulin by the enzyme inulinase.

Independent predictors and thresholds of in vitro fertilization outcomes in patients with diminished ovarian reserve.

This study aimed to identify the independent predictors of in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) outcomes among patients with diminished ovarian reserve (DOR), focusing on factors that predict the retrieval of oocytes (cumulus-oocyte complexes [COC]), day 3 (D3) available cleavage-stage embryos, clinical pregnancy during the IVF/ICSI fresh embryo transfer cycle, and viable blastocyst formation. We retrospectively analyzed 1,403 IVF/ICSI cycles involving 1,039 patients diagnosed with DOR, of which 441 cycles underwent fresh embryo transfer. Patients were categorized into groups based on their IVF/ICSI outcomes, which included oocyte retrieval, obtaining D3-available cleavage-stage embryos, clinical pregnancies, and viable blastocyst formation.

Audiological characteristics following gene therapy in patients with autosomal recessive deafness 9.

Background: Gene therapy shows promising potential for patients with autosomal recessive deafness 9 (DFNB9), with ongoing clinical trials (ChiCTR2200063181). A deeper understanding of changes in audiological characteristics is crucial for optimizing the monitoring and evaluation of patients' recovery post-treatment.

Methods: Audiological data were collected from 10 DFNB9 patients who underwent gene therapy, including auditory brain stem response (ABR), auditory steady-state response (ASSR), distortion product otoacoustic emission (DPOAE), and pure-tone audiometry (PTA) tests.