Tegoprazan-based versus esomeprazole-based triple therapy plus bismuth for first-line Helicobacter pylori eradication: A nationwide, multicenter, double-blind, double-dummy, randomized controlled trial.

Background: Although triple therapy plus bismuth (TTPB), comprising a proton pump inhibitor (PPI), two antibiotics, and bismuth, is widely used to eradicate Helicobacter pylori (H. pylori), eradication rates have been suboptimal. Potassium-competitive acid blockers (P-CABs) offer stronger and more stable gastric acid inhibition compared to PPIs.

Targeting Talin1 to reprogram dendritic cell activation landscapes: A mechanistically grounded cell therapy for multiple sclerosis model.

This study investigated the role of Talin1 in regulating dendritic cell (DC) activation and the neuroprotective benefits of Talin1-knockdown DCs in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). Bone marrow-derived DCs (BMDCs) were transduced with shTalin1 lentiviral vectors in vitro. Their morphological and biochemical profiles, surface molecules expression, cytokines production, capacity to induce T cell responses, as well as regulatory mechanisms, were comprehensively assessed.

A novel comprehensive strategy for research on wine-processed mechanism of corni fructus guided by variation in the chemical components and network analysis.

Background: Corni Fructus, a plant with both medicinal and edible properties, is renowned for its aromatic flavor and hepatoprotective and renoprotective effects, offering significant nutritional value and broad application potential. Wine-processed Corni Fructus (WCF) is a primary processed form of Corni Fructus, widely utilized in food supplements. Traditional Chinese medicine suggests that wine-processing enhances its hepatoprotective and renoprotective properties, yet the underlying mechanisms remain unclear.

Systematic Analysis of an Immune-Related Gene Signature for Predicting Prognosis and Immune Characteristics in Primary Lower Grade Glioma.

Immune-related genes (IRGs) have been increasingly recognized as critical determinants in the multistage processes of cancer development and progression. However, the functional roles of IRGs in the incidence and progression of LGG remain to be studied. This study is aimed at establishing a robust IRGs signature through systematic bioinformatics analysis, followed by an in-depth investigation of the molecular mechanisms underlying its functional roles.

Interleukin-22 promotes cancer stemness and chemotherapy resistance in colorectal cancer epidermal growth factor receptor/extracellular signal-regulated kinase pathway.

Background: Interleukin-22 (IL-22) belongs to the IL-10 cytokine family, recognized for its ability to modulate diverse immune responses. Previous studies have indicated that IL-22 promotes cancer advancement and metastasis. However, the precise function of IL-22 in colorectal cancer (CRC) remains unclear.

Vessel Shrinkage and Stroke Recurrence in Intracranial Atherosclerotic Stenosis Under Intensive Lipid-Lowering Therapy.

Background: Little is known about the dynamic process of the outer-wall boundary during intensive lipid-lowering therapy in patients with intracranial atherosclerotic stenosis and its clinical implications.

Methods: We analyzed patients with first-ever acute ischemic stroke attributed to intracranial atherosclerotic stenosis who received intensive lipid-lowering therapy with high-dose statins and PCSK9i (proprotein convertase subtilisin/kexin type 9 inhibitor). Data were obtained from a multicenter cohort study at 15 hospitals across China and our institutional database in Beijing, China.

Clinical and molecular spectrum of patients with methylmalonic acidemia and homocysteinemia complicated by cardiovascular manifestations.

Background: To investigate the clinical characteristics, treatment response, and prognosis of patients with methylmalonic acidemia (MMA) and homocysteinemia complicated by cardiovascular manifestations and to raise awareness regarding MMA and homocysteinemia.

Methods: A total of 16 children diagnosed with MMA and homocysteinemia with cardiovascular manifestations who were admitted to the Department of Pediatrics of the Second Hospital of Hebei Medical University from June 2018 to October 2024 were retrospectively analyzed.

Results: All 16 patients had varying degrees of neurological manifestations, and all had cardiovascular manifestations, 3 patients were diagnosed with MMA and homocysteinemia by newborn screening and received conventional treatment, the remaining 13 patients had nausea, vomiting, anemia, recurrent pneumonitis, respiratory distress, and lethargy as their first symptoms.

Three-Dimensional Bioprinting and Infertility-Related Female Reproductive System Diseases: A Review of Current and Future Applications.

Background: Infertility has a significant impact on women, affecting them both mentally and physically. Some of the current infertility-related diseases include intrauterine adhesions, endometriosis, polycystic ovary syndrome, primary ovarian insufficiency, and cancer. While existing treatments can help slow diseases progression and improve fertility for some patients, overall recovery rates remain low.

Comparing the diagnostic efficacy of [F]FDG PET/CT and [F]FDG PET/MRI in breast cancer recurrence: a systematic review and meta-analysis.

Purpose: This meta-analysis evaluates and compares the diagnostic accuracy of [F]FDG PET/CT and [F]FDG PET/MRI in detecting breast cancer recurrence.

Methods: A search was conducted across PubMed, Web of Science, and Embase databases up to June 10, 2025, to identify studies evaluating the diagnostic performance of [F]FDG PET/CT and/or [F]FDG PET/MRI in breast cancer recurrence. Sensitivity and specificity were calculated using the DerSimonian and Laird method with Freeman-Tukey double arcsine transformation.

[Interaction of α-amylase and inflammatory response in patients with ventilator-associated pneumonia and their prognostic value].

Objective: To investigate the interaction between α-amylase (α-AMS) and inflammatory response in patients with ventilator-associated pneumonia (VAP) and their predictive value for prognosis.

Methods: A prospective cohort study was conducted. Patients with mechanical ventilation who were treated in the intensive care unit (ICU) of the Second Hospital of Hebei Medical University from June 2020 to June 2023 were enrolled, and the patients were divided into VAP group and non-VAP group according to whether VAP occurred.

Metabolomics profiling of plaque enhancement in ischemic stroke patients with large artery intracranial atherosclerosis.

Background: Vessel wall plaque high enhancement (HE) may be regarded as an unstable and vulnerable feature of atherosclerosis. We aimed to find differential serum metabolites in ischemic stroke patients with and without HE plaques and establish models to predict plaque enhancement.

Methods: Sixty participants were recruited and divided into HE and non-HE groups.

The immune regulation and signaling transduction of FAM134B-mediated endoplasmic reticulum-phagy in ferroptosis of dendritic cells after sepsis.

Introduction: Fam134B-mediated endoplasmic reticulum (ER)-phagy is one of the key mechanisms for maintaining functional homeostasis of ER and cell survival. In fact, Fam134B-mediated ER-phagy has been proved to exert a double-edged sword in response to inflammatory and oxidative stress, which is evidenced by the recovery effect on an appropriate organellar autophagy whereas the activation of cell death in excessive condition. However, its potential role and significance in regulating the immunoreaction within the framework of sepsis and its associated mechanisms remain to be elucidated.

METTL1 drives glioma progression by promoting N7-methylguanosine (m7G) modification of glycolysis-related enzyme PGK1.

Glioma is a common brain malignancy with a poor prognosis. N7-methylguanosine (m7G) modification is involved in cancer progression, and methyltransferase 1 (METTL1) is a m7G methyltransferase. Here, we aimed to study the role of METTL1 in glioma and the potential mechanism.

Natural Orifice Specimen Extraction Surgery yields superior long-term oncological outcomes compared to traditional laparoscopic surgery in stage II-III rectal cancer.

Objectives: To compare the long-term oncological and clinical outcomes of natural orifice specimen extraction surgery (NOSES) versus traditional laparoscopic surgery (TLS) in patients with Stage II-III rectal cancer.

Methods: This retrospective cohort study analyzed data from 320 patients who underwent curative resection for Stage II-III rectal cancer between January 2020 and January 2025. Of these, 162 received NOSES and 158 underwent TLS.

EZH2-mediated PHLDA1 governs mitochondrial dysfunction and oxidative damage in traumatic brain injury via the AKT/Nrf2/Sirt3 pathway.

Objective: This study investigates the role of pleckstrin homology-like domain family A member 1 (PHLDA1) in traumatic brain injury (TBI) and examines how its knockdown may mitigate neurological impairments associated with TBI, focusing on mitochondrial dysfunction, neuro-inflammation, and oxidative stress.

Methods: TBI was induced in rats, and PHLDA1 expression was assessed through qPCR and Western blot. Neurological functions were evaluated via grip strength, balance beam, and rotarod tests.

The risk factors of bleeding after EUS-guided transmural drainage of pancreatic fluid collections: a single-center experience in China.

Background: Endoscopic ultrasonography (EUS) guided transmural drainage has become a first-line treatment for peripancreatic fluid collections (PFCs). Post-procedure bleeding may lead to severe clinical outcomes.

Aim: The purpose of this study was to explore the patient-related and surgery-related factors associated with post-EUS drainage bleeding.

Impact of early β-blocker use on the incidence of sepsis and clinical outcomes following cardiac surgery: a retrospective cohort study.

Background: Sepsis after cardiac surgery represents a severe perioperative complication with high incidence and mortality rates. While the cardioprotective benefits of β-blocker following cardiac surgery are widely recognized, their impact on sepsis development remains unclear. This study aims to investigate the association between early postoperative β-blocker use and the incidence of sepsis, as well as clinical outcomes, in patients undergoing cardiac surgery.

HSF1 in macrophages suppressed the progression of asthma via modulating SIRPα/SHP2-Dectin-1/ SYK mediated ROS and inflammatory responses.

HSF1, SIRPα, and Dectin-1 play crucial roles in immune regulation and inflammatory responses, their rols in asthma remained unclear, thereby the study was carried on. Twenty-one SPF-grade C57BL/6 mice were randomly divided into three groups: sham group, Model group, and Model + HSF1A group, with seven mice in each group. Except for the sham group, the other two groups were induced with OVA to establish an asthma model.

Human Umbilical Cord Mesenchymal Stem Cells Modulate Cytokine Secretion of CD4 T Cell in Systemic Lupus Erythematosus by Inhibiting HSP90AA1 in the Glucose-Activated PI3K-AKT Pathway.

Objective: Treatment with human umbilical cord mesenchymal stem cells (hUC-MSCs) attenuated the clinical manifestations of systemic lupus erythematosus (SLE). We investigated the metabolic mechanism whereby hUC-MSCs modify CD4 T cell cytokine secretion in lupus.

Methods: The study enrolled 30 untreated lupus patients and 20 sex, age, and body mass index matched healthy controls (HCs).

Modulating AMPA receptor-dependent synaptic plasticity: Mechanistic interventions against remifentanil-induced hyperalgesia in reoperative rats - A randomised controlled trial.

Background And Aims: Intraoperative remifentanil can induce postoperative hyperalgesia. In clinical practice, either unplanned or planned second operations may occur within a short period. However, the impact of remifentanil during this process remains unclear.

3D cell-laden scaffold printed with brain acellular matrix bioink.

Background: Intracerebral hemorrhage (ICH) is a severe neurological disorder characterized by bleeding within the brain tissue, typically associated with factors such as hypertension, cerebrovascular disease, and trauma. The transplantation of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) has demonstrated promising effects in restoring neurological function in ICH rats; however, limited retention of these cells significantly impedes their efficacy. To address this limitation, we developed a bioink composed of decellularized extracellular matrix (dECM) and hUCMSCs, which was synthesized into 3D cell-laden scaffold through 3D bioprinting.

Generation of APOE ε3/ε4 and APP-mutant human pluripotent stem cell line derived from an Alzheimer's patient.

Alzheimer's disease (AD) is a progressive neurodegenerative disorder, with mutations in the amyloid precursor protein (APP) gene and the apolipoprotein E (APOE) gene identified as significant risk factors. Here, we generated induced pluripotent stem cells (iPSCs) from an Alzheimer's patient carrying heterozygous mutations in APOE (ε3/ε4) and APP. The derived iPSCs exhibited a normal karyotype, expressed pluripotency markers, and demonstrated the capacity to differentiate into the three germ layers.

Investigation of MANF regulation of glioma stemness via STAT3/TGF-β/SMAD4/p38 pathway based on pan-cancer analysis.

Background: Glioma, particularly glioblastoma, is a highly aggressive brain tumor with poor prognosis and limited treatment options. Recent research highlights the role of MANF (Mesencephalic Astrocyte Derived Neurotrophic Factor) in tumor biology, yet its specific mechanisms in glioma remain underexplored. This study aims to elucidate the role of MANF in glioma and its underlying mechanisms of action.

[Diagnostic and treatment strategies for testicular torsion].

Objective: To investigate the differences between high-frequency ultrasound-guided manual detorsion combined with surgery (MD+S) and surgery alone in the treatment of testicular torsion, and to provide some new evidence for the timely diagnosis and treatment of the disease.

Methods: We retrospectively analyzed the clinical data on 134 cases of unilateral testicular torsion within 48 hours treated in our hospital by MD+S or by surgery alone from January 2015 to May 2022. We statistically analyzed the age distribution, and duration and degrees of testicular torsion, followed by comparison between the two groups.

Diffusion tensor image analysis along the perivascular space index and visual analogue scales in female patients with patent foramen ovale and migraine perform better after percutaneous closure.

Background: Patent foramen ovale (PFO) is more common in females and often complicates with migraine; however, its mechanism is not completely definite. This study aimed to explore whether a decline of diffusion tensor image analysis along the perivascular space (DTI-ALPS) index exists which partially indicates glymphatic function, examine the relationship between diffusivity parameters, DTI-ALPS index, and visual analogue scales (VAS), and further investigate how the DTI-ALPS index and VAS perform after percutaneous closure in patients with PFO and migraine.

Methods: Female patients with PFO and migraine without aura were prospectively included; they underwent diffusion tensor imaging (DTI) scanning and received VAS scores before and after percutaneous closure.