Neuro-Immuno-Stromal Context in Colorectal Cancer: An Enteric Glial Cell-Driven Prognostic Model via Machine Learning Predicts Survival, Recurrence, and Therapy Response.

Background: Enteric glial cells (EGCs) have been implicated in colorectal cancer (CRC) progression. This study aimed to develop and validate a prognostic model integrating EGC- and CRC-associated gene expression to predict patient survival, recurrence, metastasis, and therapy response.

Methods: Bulk and single-cell RNA sequencing data were analyzed, and a machine learning-based model was constructed using the RSF random forest algorithm.

Effect of acid-suppressive drugs on endoscopic transmural drainage of peripancreatic fluid collections-a randomized controlled trial.

Background: Acid-suppressing drugs affect intestinal microbes by inhibiting the secretion of stomach acid. However, it is not clear whether acid suppressive drugs affect the microorganisms in the peripancreatic effusion and affect the postoperative complications.

Methods: This study was a prospective randomized controlled trial.

High-Resolution Spatial Transcriptomics Unveils Spatially Resolved Gene Modules and Fatty Acid Metabolism Dysregulation in Human Skin Aging.

Human skin aging involves intricate micrometer-scale changes in cellular organization. High-resolution spatial transcriptomic analysis is crucial for deciphering the complex interplay of cell types and molecular processes underlying skin aging. Here, the Stereo-seq technique was employed to generate a high-resolution spatial transcriptomic atlas of human eyelid skin, capturing age-related alterations in gene expression.

Clinical efficacy comparison of CO2 laser treatment and LEEP surgery for cervical intraepithelial neoplasia with high-risk HPV infection.

Background: To investigate the clinical efficacy of CO2 laser and loop electrosurgical excision procedure (LEEP) in the treatment of cervical intraepithelial neoplasia (CIN) with high-risk human papillomavirus (HPV) infection.

Methods: A total of 150 CIN patients with high-risk HPV infection admitted to our hospital from December 2020 to June 2022 were divided into CO2 laser treatment group (CO2 group) with 63 cases and LEEP treatment group (LEEP group) with 87 cases based on treatment principles and patient preferences. The postoperative recovery time, CIN treatment effect, high-risk HPV clearance rate, treatment safety, and recurrence rate were compared between the two groups.

Advances in tissue optical clearing for 3D imaging in large animal.

Three-dimensional reconstruction of tissue architecture is crucial for biomedical research. Tissue optical clearing technology overcomes light scattering limitations in biological tissues, providing an essential tool for high-resolution three-dimensional imaging. Given the high degree of similarity between large model animals (e.

FTO regulates the proliferation and apoptosis of pulmonary artery smooth muscle cells through m6A demethylation modification.

Background: To investigate whether FTO-mediated N6-methyladenosine (m6A) demethylation affects the proliferative/apoptotic phenotype of mouse pulmonary artery smooth muscle cells (PASMCs).

Methods: The hypoxia model of PASMCs was established to examine changes in FTO protein expression and m6A modification levels. Cell transfection, m6A expression profiling, mRNA stability testing, and protein-RNA binding assays were used to explore the effects of FTO and its downstream target, CACNA1d, on PASMC proliferation and apoptosis.

The risk factors of bleeding after EUS-guided transmural drainage of pancreatic fluid collections: a single-center experience in China.

Background: Endoscopic ultrasonography (EUS) guided transmural drainage has become a first-line treatment for peripancreatic fluid collections (PFCs). Post-procedure bleeding may lead to severe clinical outcomes.

Aim: The purpose of this study was to explore the patient-related and surgery-related factors associated with post-EUS drainage bleeding.

Avicularin induces apoptosis in NSCLC by promoting USP7-mediated degradation of FOXM1.

Non-small cell lung cancer (NSCLC) remains a leading cause of cancer-related mortality worldwide, underscoring the urgent need for novel and effective therapeutic strategies. Avicularin (Avi), a naturally occurring flavonoid, has been shown to reduce cell viability and induce caspase-dependent apoptosis in NSCLC cells, while exhibiting minimal cytotoxicity toward normal bronchial epithelial cells. Mechanistically, Avi selectively decreases FOXM1 protein expression without affecting its mRNA levels, suggesting post-transcriptional regulation.

ISGylation of zebrafish STING at lysines 221 and 276 activates innate immunity.

The interferon gene stimulator (STING) is an endoplasmic reticulum (ER)-resident protein that plays a crucial role in the immune response to microbial infections. ISGylation, a form of post-translational modification, regulates innate immunity in mammals. However, the regulatory mechanism of ISGylation on STING in fish remains largely unknown.

Targeted delivery of liposomal senolytics to alleviate cellular senescence-induced bone loss.

The senescence of bone marrow-derived mesenchymal stem cells is involved in osteoporosis. The combination of dasatinib and quercetin has been explored to alleviate bone loss by efficiently reducing senescent cell populations. However, senolytic therapy by dasatinib and quercetin requires a precise ratio for better therapeutic effects, which is hard to achieve by oral administration.

Chain Length-Dependent Reverse Micelle Extraction of Prolamins From Baijiu Jiuzao: Synergistic Effects of Ethanol, Ionic Strength, and Chaotropic Agent.

This study investigated the chain length-dependent extraction efficiency of two cationic surfactants-dodecyltrimethylammonium bromide (C12, 12-carbon chain) and cetyltrimethylammonium bromide (C16, 16-carbon chain)-for reverse micelle extraction of prolamins from Baijiu Jiuzao (Chinese Baijiu distillers' grains). Although pH variations (4.0-9.

Comparative Analysis of the Mitochondrial Genomes of Five Species of (Orthoptera: Anostostomatidae) and the Phylogenetic Implications of Anostostomatidae.

In China, Anostostomatidae is represented by a single tribe, Anabropsini; two genera; and 33 species. Although extensive research has been conducted on Anabropsini, the monophyly of this tribe within Anostostomatidae remains unverified. Furthermore, the phylogenetic relationships within remain under debate.

Liquid Metal-Based Capacitive Strain Sensor with Self-Shielding and Low-Hysteresis.

Soft capacitive strain sensors with soft conductive electrodes are advantageous for their ability to decouple resistance from the flexible electrodes, offering excellent repeatability, low hysteresis, low energy consumption, and good temperature stability. However, existing soft capacitive strain sensors utilize intrinsically stiff conductors as soft electrodes, limiting the sensitivity, repeatability, and hysteresis. To address these issues, this work proposes a soft capacitive strain sensor based on liquid metal.

Simulation of coal resistivity dynamics during methane adsorption and desorption using an electrical rock physics model.

Understanding the correlation between coal resistivity and methane content is critical for optimizing coalbed methane (CBM) recovery and ensuring mining safety. Existing studies mainly rely on empirical trend fitting, leaving a gap in model-driven analyses of resistivity dynamics during methane adsorption and desorption. This study develops a dual-coefficient electrical rock physics model integrating inorganic mineral composition, organic resistivity, methane adsorption-desorption behavior, and pore inclusion structures.

Identification and validation of a ferroptosis-related long non-coding RNA signature as a prognostic biomarker for hepatocellular carcinoma.

Background: Ferroptosis may play a central role in the development of hepatocellular carcinoma (HCC). Ferroptosis-related long non-coding RNAs (FRlncRNAs) show prognostic value in HCC through ferroptosis regulation and significant survival correlation. This study aimed to identify a prognostic FRlncRNA signature and explored its association with tumor immunity and oncogenic pathways.

Using Metal-Organic Framework Nanoparticles for Targeted Codelivery of Bortezomib and Iron Ions to Mitochondrial TOM20 to Induce Ferroptosis for Colorectal Cancer Treatment.

Targeted promotion of iron ion accumulation and inhibition of outer membrane protein function in mitochondria contribute to ferroptosis, thereby boosting anticolorectal cancer (CRC) efficacy. Based on our previous study, the anticancer agent bortezomib (BTZ) was loaded in an iron-derived metal-organic framework (MOF), which was further modified by rhodamine B (RhB), yielding BTZ@RhB-MOF for targeted CRC treatment. Physicochemical characterization results indicated successful preparation of BTZ@RhB-MOF, which had the framework structure and nanosize properties with BTZ and iron ion release under acidic conditions.

An enzyme-responsive hydrogel functionalized with mesoporous silica nanoparticles for co-delivery of cisplatin and shRNA to overcome chemotherapy resistance in non-small cell lung cancer.

Chemoresistance poses a critical challenge in cancer therapy across diverse tumor types, including non-small cell lung cancer (NSCLC), where chemotherapy-induced neuroendocrine differentiation (NED) of tumor cells plays a pivotal role in acquiring treatment resistance. This process significantly reduces chemotherapy efficacy, accelerates tumor progression, and ultimately worsens patient survival outcomes. The complex mechanisms underlying chemoresistance involve multiple factors, including enhanced DNA repair pathways, increased drug efflux capacity, and alterations in gene expression.

polysaccharides ameliorate metabolic-associated fatty liver disease by decreasing the NLRP3 inflammasome and pyroptosis.

Background: The role of nucleotide-binding oligomerization domain-like receptors containing pyrin domain 3 (NLRP3) inflammasome and pyroptosis in the inflammatory microenvironment of metabolic-associated fatty liver disease (MASLD) has been posited as crucial. polysaccharides (BSPs), extracted from the tubers of , exhibit significant anti-inflammatory properties. However, their potential protective effects on MASLD and their role in regulating pyroptosis remain unclear.

[Clinical phenotype and genetic analysis of four cases of Epileptic encephalopathy caused by PCDH19 mutations].

Objective: To investigate the clinical phenotype and genotype features of children with Epileptic encephalopathy caused by PCDH19 mutations.

Methods: Four children with epilepsy caused by PCDH19 gene mutations who were treated at Shanghai Children's Medical Center from August 2015 to May 2024 were selected as study subjects. A retrospective study method was used to collect the clinical data of the patients.

A lyophilized anti-rabies mRNA-LNP vaccine induces early and robust immune responses from a single-dose subcutaneous administration.

Rabies is a zoonotic disease caused by the rabies virus (RABV). RABV infections cause severe destruction to the central nervous system with fatal consequences, which has prompted global efforts to develop a highly effective and safe vaccine. Currently, the most widely used vaccines are inactivated vaccines, which need multiple injected doses for either pre-exposure prophylaxis or post-exposure immunization.

Effects of motion sickness patch on postoperative nausea and vomiting in female patients undergoing thyroid surgery: a randomized clinical trial.

Background: This study aims to investigate whether the application of motion sickness patch can effectively reduce the incidence of PONV (Postoperative nausea and vomiting) in female patients undergoing thyroid surgery.

Methods: One hundred and sixty-two female patients undergoing thyroid surgery were randomly assigned into motion sickness patch group and control group. Both groups received standard prophylactic antiemetic therapy with intravenous dexamethasone (8 mg) and palonosetron (0.

Effectiveness of Watching Cartoons in Reducing Pain in Children Receiving Vaccine Injection: A Randomized Controlled Trial.

Background: Vaccination is a highly effective method for preventing a variety of infectious diseases; however, the pain associated with vaccination in children is often overlooked. This study primarily aims to explore whether watching cartoons can reduce the pain experienced by children during vaccine injection.

Methods: In this randomized controlled trial, 72 children aged 1 to 6 years were recruited with parental consent.

RBCK1 enhances antiviral response through promoting K63-linked polyubiquitination of IRF7 in Carassius auratus var. Pengze.

Ubiquitination is a widely occurring reversible post-translational modification process in cells. RANBP2-type and C3HC4-type zinc finger-containing 1 (RBCK1) is a kind of E3 ubiquitin ligase. It regulates various biological functions, including protein degradation and kinase signaling pathways in mammals.

Ctenopharyngodon idellus Mex3C activates the innate immune by promoting the K63-linked ubiquitination of RIG-I.

The cytoplasmic RNA sensor RIG-I-like receptors (RLRs) play a pivotal role in initiating antiviral innate immunity. In this study, we identified the grass carp (Ctenopharyngodon idellus) Mex3C gene, named CiMex3C which encodes a protein with a RING domain and two heterogeneous nuclear ribonucleoprotein K homology (KH) domains. Our study revealed that CiMex3C responded to multiple stimuli including GCRV, poly(I:C), B-DNA, Z-DNA, characterized by the heightened sensitivity to GCRV stimulation.

Whole exome sequencing unravels genetic architecture and its clinical implications in pediatric pulmonary arterial hypertension.

Background: Pulmonary arterial hypertension (PAH) is a severe disease with significant genetic predisposition. While genetic architecture and clinical implications in pediatric PAH remain unclear.

Methods: We retrospectively analyzed clinical and genetic data from 218 pediatric PAH patients including 115 idiopathic/heritable PAH (IPAH/HPAH) and 103 PAH associated with congenital heart disease (PAH-CHD) admitted to our center between 2011 and 2023.