The Evolution of Virulence of Carbapenem-Resistant from the Same Source Under the Pressure of Omadacycline Treatment.

Introduction: (KP) is a common Gram-negative bacterium in clinical practice and can cause various infectious diseases, including pneumonia, liver abscess and bloodstream infection. Carbapenem-resistant (CRKP) has become a major threat to global health due to its high incidence and mortality rates, especially the ST11-CRKP strain prevalent in China.

Methods: The age, main clinical diagnosis, previous health and immune status of the two patients with ST11-CRKP-related infections during the same period reported in this study were similar.

Correlation between thyroid sensitivity indices and bone metabolism in newly diagnosed middle aged and elderly patients with type 2 diabetes mellitus with normal thyroid function.

Objective: To investigate the association between thyroid hormone sensitivity indices and bone metabolism markers in newly diagnosed middle-aged and elderly type 2 diabetes mellitus (T2DM) patients with normal thyroid function.

Method: We retrospectively analyzed 350 newly diagnosed T2DM patients (≥ 45 years), stratified by bone mineral density into Group A (normal bone density group) and Group B (low bone mass and osteoporosis group). General data and clinical biochemical parameters were collected: free triiodothyronine (FT3), free thyroxine (FT4), thyroid-stimulating hormone (TSH), 25-hydroxyvitamin D (25(OH)D), parathyroid hormone (PTH), osteocalcin (OC), bone-specific alkaline phosphatase (BALP), serum calcium (Ca), serum phosphorus (P), fasting plasma glucose (FPG), glycosylated hemoglobinA1c (HbA1c), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), serum creatinine (SCr), serum uric acid (SUA), and estimated Glomerular Filtration Rate (eGFR).

Vagus Nerve Stimulation Attenuates Cognitive Impairment in Traumatic Brain Injury via the mtDNA/cGAS-STING/NLRP3 Inflammasome Axis.

Background: Traumatic brain injury (TBI) is a major life-threatening event. In addition to neurological deficits, it can lead to long-term impairments of cognitive function. The vagus nerve (VN) provides a direct communication conduit between the central nervous system and the periphery, and modulation of the inflammatory reflex via electrical stimulation of the vagus nerve (VNS) shows efficacy in ameliorating pathology in neurodegenerative diseases.

Clinical gene therapy restores hearing: a paradigm shift.

Recent breakthroughs in gene therapy for autosomal recessive deafness 9 (DFNB9) caused by OTOF mutations have transformed treatment paradigms for hereditary hearing loss (HHL). To date, eight clinical trials targeting DFNB9 have been registered in 51 centers across eight countries, demonstrating the rapid progress of gene therapy in auditory medicine. These pioneering studies establish the framework for the clinical translation of gene therapy targeting HHL.

All-trans retinoic acid exacerbates Stevens-Johnson syndrome and toxic epidermal necrolysis via TNF signaling pathways: A network toxicology, molecular docking, and experimental study.

Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are severe hypersensitivity reactions characterized by extensive epidermal necrosis, often induced by medications. This research aims to investigate the involvement of all-trans retinoic acid (ATRA), a derivative of vitamin A known to induce dermatological toxicity resembling SJS/TEN, in the development of these conditions. Utilizing network toxicology methodologies, molecular docking technology, and experimental validation, we identified 159 common targets between ATRA and SJS, 38 with TEN, and 27 shared among all three conditions through databases such as SwissTargetPrediction and GeneCards.

First-line benmelstobart plus anlotinib versus sunitinib in advanced renal cell carcinoma (ETER100): a multicentre, randomised, open-label, phase 3 trial.

Background: The underexplored potential of PD-L1 blockade in advanced renal cell carcinoma highlights an urgent need for novel agents. This trial aimed to compare benmelstobart (a novel PD-L1 inhibitor) plus anlotinib with sunitinib as first-line treatment for advanced renal cell carcinoma.

Methods: ETER100 was a multicentre, randomised, open-label, phase 3 trial conducted at 37 medical sites in China.

SEE-phAST: Spatially encapsulated emulsions for phenotypic antibiotic susceptibility testing via sequential digital RAA-CRISPR.

The escalating threat of antimicrobial resistance is exacerbated by delayed diagnostics and improper antibiotics use, underscoring an urgent demand for rapid, versatile AST tools to support evidence-based prescribing. In this study, we present an innovative, generalizable phenotypic AST approach by quantifying bacterial gDNA copy number variations (CNVs) following 0.5-h-brief culturing with antibiotic exposure, termed spatially encapsulated emulsions (SEE)-phAST.

Technological evolution and research frontiers of robot-assisted ultrasound examination: a bibliometric exploration.

Technological innovations in robot-assisted ultrasound (RAUS) have remarkably advanced the development of precision and intelligent medical imaging diagnosis. This study aims to use bibliometric methods to systematically analyze the technological evolution and research frontiers in the RAUS field, providing valuable insights for future research. This study used the Web of Science Core Collection database to retrieve English-language research papers and reviews related to RAUS published between 2000 and 2024.

The evolution of concomitant mitral regurgitation in patients with severe aortic stenosis undergoing transcatheter aortic valve replacement: a prospective multi-center China-DVD2 cohort study.

Background: Severe aortic stenosis (AS) often coexists with mitral regurgitation (MR). This study evaluated the significant evolution of MR in patients undergoing transcatheter aortic valve replacement (TAVR) for AS.

Methods: This multi-center China Degenerative Valve Disease II Cohort (China-DVD2) Study enrolled patients undergoing TAVR for AS from January 2020 to October 2023.

Dietary Citrate Restores Age-Related Endothelial Cell Mitochondrial Dysfunction and Alleviates Atherosclerosis.

Vascular aging increases the susceptibility to cardio-cerebrovascular conditions, such as atherosclerotic diseases and hypertension, the leading causes of global disability and mortality. Dietary citrate extends the lifespan of Drosophila melanogaster and Caenorhabditis elegans as well as improves the memory of mice injured by a high-fat diet (HFD); whether it alleviates vascular aging and age-related vascular diseases; however, remains unknown. Here, we showed that dietary supplementation of citrate delayed vascular aging, as evidenced by maintaining the integrity of elastic fibers and decreasing the level of the aging-related marker, CDKN1A (p21).

Engineered hollow CuO@ZnO p-n heterojunction nanocomposites for synergistic photocatalytic disinfection and tissue regeneration.

Photocatalytic antibacterial therapy is a promising method for wound disinfection and treatment. However, the weak photocatalytic antibacterial activity of ZnO stimulated by visible light limits its applications. In this study, porous CuO@ZnO heterojunctions with enhanced visible light response are successfully synthesized by coupling ZnO and CuO using a one-pot water bath method.

Smartphones as Catalysts for Synergistic Nutrition: A New Era in Bioactive Detection, Personalization, and Food System Intelligence.

Naturally occurring bioactive compounds such as polyphenols, flavonoids, and vitamins play critical roles in human health and sustainable food systems. Yet their widespread utilization is constrained by complex detection methods and limited accessibility. This review explores how smartphones are emerging as transformative platforms for real-time analysis, enhanced synergy discovery, and personalized nutrition.

Exploration Progress on Inflammatory Responses and Immune Regulatory Mechanisms in Diabetic Retinopathy.

Diabetic retinopathy (DR) is one of the most common microvascular complications of diabetes. In recent years, with the number of diabetic patients increasing annually, the number of DR patients has also been rising, and it has become one of the major blinding eye diseases worldwide. The pathogenesis of DR has not been fully clarified but includes microvascular lesions and metabolic factors.

Predictive value of the stone-free rate after percutaneous nephrolithotomy based on multiple machine learning models.

Purpose: This study aimed to develop three types of machine learning (ML) models based on gradient boosting decision tree (GBDT), random forest (RF), and extreme gradient boosting (XGBoost) to explore their predictive value for the stone-free rate after percutaneous nephrolithotomy (PCNL).

Patients And Methods: A retrospective analysis was conducted on 160 patients who underwent PCNL. The patients were randomly divided into a training set and a test set in a 7:3 ratio.

Targeted Inhibition of NOTCH2 and Importin-β Promotes Osteogenic Differentiation of Osteosarcoma Cells.

Osteosarcoma (OS) is a common malignant bone tumor, frequently associated with impaired osteogenic differentiation of tumor cells. Recent studies have suggested that the NOTCH signaling pathway plays a crucial role in maintaining tumor cell stemness and may influence their differentiation status. This study investigates the role of NOTCH2, a key receptor in the NOTCH family, in regulating osteogenic differentiation in OS.

[Effect of mouse nerve growth factor combined with neostigmine on nerve repair after envenoming bites by Bungarus multicinctus].

To evaluate the efficacy and clinical application value of mouse nerve growth factor combined with neostigmine in the treatment of nerve injury in patients bitten by Bungarus multicinctus by a prospective single-blind controlled clinical study. In June 2020, a prospective, single-blindness, randomized grouping method was used to select patients with bungarus multicinctus saurogobio dabryi subsp. dabryi bite injuries admitted to the Emergency Department of Ganzhou People's Hospital in Jiangxi Province as the study subjects.

Ambient air pollution exposure, mediating biomarkers and risk of COPD: a cohort study and meta-analysis.

Background: Limited evidence exists on air pollution's systemic impact on lung function and mediating biomarkers. This study comprehensively evaluated associations between air pollution, COPD and lung function, while exploring biomarker mediation.

Methods: A prospective analysis of 451 566 UK Biobank participants was conducted.

Changes in dynamic and static functional connectivity in amygdala subregions in major depressive disorder treated with esketamine in and sertraline: A pilot study.

Introduction: Dysfunction in amygdala networks has been implicated in major depressive disorder (MDD). Pharmacological treatments, such as esketamine and sertraline, are believed to exert their antidepressant effects by modulating amygdalar activity. This study aimed to investigate the relationship between changes in dynamic functional connectivity (dFC) within amygdala subregions and treatment outcomes, with a focus on identifying potential neuroimaging markers.

Nano-enabled repurposing of Benproperine phosphate enhances pancreatic Cancer chemotherapy through lethal autophagy arrest and immune activation.

Gemcitabine-based chemotherapy remains a cornerstone in pancreatic cancer treatment, yet its efficacy is hindered by poor bioavailability and adaptive resistance mechanisms, such as autophagy. In this study, we developed a hyaluronic acid (HA) modified zeolitic imidazolate framework-8 (ZIF-8) drug-repurposing nanoplatform (HA/ZIF-8@BPP/Gem) against pancreatic cancer through the co-delivery of the antitussive benproperine phosphate (BPP) and gemcitabine (Gem). Using cell lines, patient-derived xenograft models, and orthotopic tumor models, we demonstrated that BPP and Gem, rapidly released from the nanoplatform in the acidic tumor microenvironment, exhibited synergistic cytotoxicity without causing significant biochemical abnormalities or organ toxicity.

Effect of linusorbs on oxidative stability of lipid systems with different unsaturation and the antioxidant mechanism.

The antioxidant capacity and mechanism of linusorbs (LOs) on lipid systems with different unsaturation was unclear. This study aimed to explore the oxidative stability of flaxseed oil (FO), walnut oil (WO), and camellia oil (CO) in the presence of LOs in order to recognize the impacts of unsaturation degree of the lipid systems on the performance of natural antioxidants. By the evaluation of fatty acid compositions, PV/CD and AV/p-AV value, and changes of characteristic peaks in Raman spectra, LOs can inhibit oil oxidation in different lipid systems, especially potent for C18:3-based FO, followed by C18:2-based WO, and C18:1-based CO.

VIPR1 induces cuproptosis and inhibits the HIF-1α pathway in colon cancer.

Cuproptosis, a recently characterized form of programmed cell death, has been implicated in tumor progression; however, its specific role in colon cancer remains poorly understood. This study aims to elucidate the potential involvement of cuproptosis-related genes in the development and progression of colon cancer. Differentially expressed genes (DEGs) associated with cuproptosis in colon cancer were identified through bioinformatics analysis of the GSE4183 and GSE74602 datasets.

Tissue metabolomics reveals metabolic dysregulation associated with intimal hyperplasia in arteriovenous fistula stenosis.

Objective: This study performed untargeted LC-MS metabolomics on venous tissues from maintenance hemodialysis patients undergoing arteriovenous fistula (AVF) reconstruction surgery.

Methods: A total of six stenotic and six non-stenotic AVF tissues were analyzed. Paired samples were collected from stenotic AVF segments and non-stenotic regions (control group).