Neuroprotective role of GLT-1/EAAT2 in glutamate-induced excitotoxicity.

Glutamate-mediated excitotoxicity represents a common pathomechanism in neurological disorders. As the predominant glutamate transporter in the central nervous system, glutamate transporter 1 (GLT-1, known as EAAT2 in humans) plays a crucial role in maintaining glutamate homeostasis and preventing excitotoxicity through its Na⁺-dependent transport mechanism. Key functions of GLT-1 include reducing extracellular glutamate concentration, regulating calcium homeostasis, suppressing oxidative stress, preserving mitochondrial integrity, and modulating neuroinflammatory processes by limiting microglial activation.

HCN2 promotes neurodevelopmental and synaptic function repair through the CaMKII/CREB pathway to alleviate general anesthesia-induced cognitive impairment.

Repeated exposure to gestational general anesthesia during pregnancy has been associated with neurodevelopmental damage and cognitive and social dysfunction in offspring. This study investigates the underlying mechanisms and therapeutic strategies for mitigating these effects. Behavioral tests revealed significant impairments in cognitive, social, and spatial learning abilities in the offspring of general anesthesia-treated mice, alongside delayed eye-opening, reduced body weight, and neuronal damage.

Alpinetin protects against myocardial ischemia-reperfusion injury by inhibiting ferroptosis and apoptosis via mitochondrial ferritin.

Purpose: Ischemia-reperfusion injury remains a major problem following myocardial infarction. Alpinetin (ALPT) has been reported to exhibit cardioprotective effects as well as resistance to ischemia-reperfusion injury. However, its role and mechanism during myocardial ischemia-reperfusion injury are unknown.

Early Diastolic Dysfunction Detection in Hypertension: CMR-Derived Left Atrial Strain.

Rationale And Objectives: This study aimed to investigate the role of cardiac magnetic resonance (CMR)-derived left atrial (LA) strain parameters in evaluating early cardiac dysfunction in hypertensive patients and to assess their diagnostic utility for left ventricular diastolic dysfunction (LVDD).

Methods: A total of 150 hypertensive patients and 60 healthy controls were retrospectively enrolled, with all participants undergoing both echocardiographic and CMR examinations. Hypertensive patients were stratified by LVDD severity based on current guidelines.

Dietary non-starch plant polysaccharides: Multi-mechanisms for managing diabetic microvascular complications.

The global burden of diabetes has been exacerbated by a shift in dietary patterns toward diets rich in refined sugars, saturated fats and energy-dense nutrients. Diabetes is a metabolic disease characterized by chronic hyperglycemia. Persistently elevated blood glucose levels can lead to microvascular complications that contribute greatly to reduced quality of life, disability or death.

Age-group variations in the associations between technostress, job burnout, and work engagement among nurses: Evidence from a network analysis.

Background: While digital transformation has become a necessary trend for hospitals, it imposes technostress on nurses working on the health-care front lines. According to previous research, it increases conflict between nurses and patients but the mechanism and age difference are ignored.

Methods: A total of 672 nurses recruited from five hospitals in Liaoning Province, China.

Effect of henagliflozin on aging biomarkers in patients with type 2 diabetes: A multicenter, randomized, double-blind, placebo-controlled study.

Sodium-glucose cotransporter-2 inhibitors have been proposed as caloric restriction mimetics with potential anti-aging effects. However, clinical data on their influence on aging biomarkers are limited. In this multicenter, randomized, double-blind, placebo-controlled trial, 150 participants with type 2 diabetes are randomized (1:1) to receive oral henagliflozin (10 mg/day) or placebo for 26 weeks.

Effects of imperatorin on chronic periodontitis associated with type 2 diabetes mellitus.

Aim: Periodontitis is a common complication of type 2 diabetes. Imperatorin is a traditional Chinese medicinal compound recognized for its anti-inflammatory, analgesic and anti-tumor properties. Nevertheless, the impact of imperatorin on chronic periodontitis and chronic periodontitis associated with type 2 diabetes mellitus remains unexplored.

Efficacy and safety of intranasal corticosteroids combined with mucoactive drugs for chronic rhinosinusitis.

Background: Chronic rhinosinusitis (CRS) leads to a burden in life and economy. Better therapies need to be explored.

Objective: This stage I study aims to explore the efficacy and safety of intranasal corticosteroids combined with mucoactive drugs for CRS.

A single-molecule biosensor based on multiple fluorophore nucleic acid probe and STORM imaging for detection of Hantaan virus.

Hemorrhagic fever with renal syndrome (HFRS), caused by Hantaan virus, poses a serious public health threat. Current diagnostic methods remain limited by low sensitivity, complex procedures, and high sample requirements. To address this, we developed a highly sensitive single-molecule biosensor using multi-fluorophore nucleic acid probes and STORM imaging for the detection of Hantaan virus RNA.

Comparative analysis of endplate volumetric bone mineral density and endplate vertebral bone quality for predicting cage subsidence in lateral lumbar interbody fusion.

Objective: The aim of this study was to compare the predictive efficacy of quantitative CT (QCT)-based endplate volumetric bone mineral density (EP-vBMD) and MRI-based endplate vertebral bone quality (EBQ) score for cage subsidence (CS) after lateral lumbar interbody fusion (LLIF).

Methods: A retrospective study was conducted on patients who underwent single-level LLIF in conjunction with pedicle screw fixation at the authors' institution between January 2019 and April 2023. The volumetric bone mineral density (vBMD) was measured based on preoperative CT using phantom-less QCT software.

Deep Eutectic Solvent: Improve the Constituents in Plantago asiatica L. (PAL) Extract Oral Absorption in Rats.

Plantago asiatica L. (PAL) is a prevalent perennial herb utilized for both culinary and medicinal purposes. This article examines the impact of deep eutectic solvent (DES, composed of choline chloride and lactic acid) on the pharmacokinetics of chemical constituents in PAL extract.

Ligand Engineering-Based High-Efficiency Photothermal Sensors Powered Sensitive Lateral Flow Immunoassay Platform.

The photothermal lateral flow immunoassay (LFIA) has garnered considerable attention, owing to its suitability for on-site quantitative detection. Furthermore, it has distinct advantages in further constructing sensitive detection methods for low-concentration targets. In this study, we employed a ligand engineering strategy to synthesize Fe-chelated quinone nanoparticles (FQNPs).

Trends of Female Breast Cancer Burden in China over 25 Years: A Join Point Regression and Age-Period-Cohort Analysis Based on the GBD (1997-2021).

Background: Breast cancer (BC) is one of the most prevalent malignant tumors among women globally. The incidence and mortality rates of female BC exhibit significant variation across different countries and regions.

Objective: This study analyzed the trends of BC among Chinese women from 1997 to 2021 to support evidence-based for the prevention, screening and treatment strategies of female BC in China.

Multiple roles and mechanisms of MUC6 in cancer (Review).

Mucin 6 (MUC6), primarily expressed in the gastrointestinal (GI) epithelium, is a member of the mucin family characterized by a protein backbone with extensive glycosylation, playing a crucial role in preserving epithelial barrier integrity. Accumulating evidence indicates that MUC6 glycosylation contributes significantly to cancer development, diagnosis, therapy and prognosis‑particularly in GI malignancies such as gastric, pancreatic and colorectal cancers. In the present review, current findings on the multifaceted roles of MUC6 across various cancers were comprehensively summarize.

Fangchinoline attenuates hepatic fibrosis by regulating taurine metabolism and oxidative stress.

Background: Hepatic fibrosis emerges as a pathological hallmark in the pathogenesis of chronic hepatopathies. is a traditional Chinese herb used to treat liver disease. However, the anti-hepatic fibrosis effect of fangchinoline (FAN), an active ingredient of , has not been reported.

Rare primary anorectal malignant melanoma presenting with painless hematochezia: a case report.

Background: Anorectal malignant melanoma (ARMM) is an exceedingly rare and highly aggressive malignancy characterized by low prevalence, high misdiagnosis rates, and frequent recurrence/metastasis.

Case Report: This report details the case of a 51-year-old woman presenting with persistent bright red blood in her stool. Digital rectal examination revealed a firm, spherical mass approximately 4 cm from the anal verge.

Epigenetic regulation of breast ductal carcinoma in situ by miR-217 through DNMT1 and Hedgehog-GLI pathway.

Ductal carcinoma in situ (DCIS) is a noninvasive precursor of breast cancer with a high potential for progression. Aberrant DNA methylation plays a pivotal role in early tumorigenesis, yet the regulatory mechanisms remain incompletely defined. Integrated bioinformatic analysis of methylation and transcriptomic datasets identified miR-217 as a candidate regulator of DNA methyltransferase 1 (DNMT1).

The Evolution of Virulence of Carbapenem-Resistant from the Same Source Under the Pressure of Omadacycline Treatment.

Introduction: (KP) is a common Gram-negative bacterium in clinical practice and can cause various infectious diseases, including pneumonia, liver abscess and bloodstream infection. Carbapenem-resistant (CRKP) has become a major threat to global health due to its high incidence and mortality rates, especially the ST11-CRKP strain prevalent in China.

Methods: The age, main clinical diagnosis, previous health and immune status of the two patients with ST11-CRKP-related infections during the same period reported in this study were similar.

Correlation between thyroid sensitivity indices and bone metabolism in newly diagnosed middle aged and elderly patients with type 2 diabetes mellitus with normal thyroid function.

Objective: To investigate the association between thyroid hormone sensitivity indices and bone metabolism markers in newly diagnosed middle-aged and elderly type 2 diabetes mellitus (T2DM) patients with normal thyroid function.

Method: We retrospectively analyzed 350 newly diagnosed T2DM patients (≥ 45 years), stratified by bone mineral density into Group A (normal bone density group) and Group B (low bone mass and osteoporosis group). General data and clinical biochemical parameters were collected: free triiodothyronine (FT3), free thyroxine (FT4), thyroid-stimulating hormone (TSH), 25-hydroxyvitamin D (25(OH)D), parathyroid hormone (PTH), osteocalcin (OC), bone-specific alkaline phosphatase (BALP), serum calcium (Ca), serum phosphorus (P), fasting plasma glucose (FPG), glycosylated hemoglobinA1c (HbA1c), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), serum creatinine (SCr), serum uric acid (SUA), and estimated Glomerular Filtration Rate (eGFR).

Vagus Nerve Stimulation Attenuates Cognitive Impairment in Traumatic Brain Injury via the mtDNA/cGAS-STING/NLRP3 Inflammasome Axis.

Background: Traumatic brain injury (TBI) is a major life-threatening event. In addition to neurological deficits, it can lead to long-term impairments of cognitive function. The vagus nerve (VN) provides a direct communication conduit between the central nervous system and the periphery, and modulation of the inflammatory reflex via electrical stimulation of the vagus nerve (VNS) shows efficacy in ameliorating pathology in neurodegenerative diseases.

Clinical gene therapy restores hearing: a paradigm shift.

Recent breakthroughs in gene therapy for autosomal recessive deafness 9 (DFNB9) caused by OTOF mutations have transformed treatment paradigms for hereditary hearing loss (HHL). To date, eight clinical trials targeting DFNB9 have been registered in 51 centers across eight countries, demonstrating the rapid progress of gene therapy in auditory medicine. These pioneering studies establish the framework for the clinical translation of gene therapy targeting HHL.

All-trans retinoic acid exacerbates Stevens-Johnson syndrome and toxic epidermal necrolysis via TNF signaling pathways: A network toxicology, molecular docking, and experimental study.

Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are severe hypersensitivity reactions characterized by extensive epidermal necrosis, often induced by medications. This research aims to investigate the involvement of all-trans retinoic acid (ATRA), a derivative of vitamin A known to induce dermatological toxicity resembling SJS/TEN, in the development of these conditions. Utilizing network toxicology methodologies, molecular docking technology, and experimental validation, we identified 159 common targets between ATRA and SJS, 38 with TEN, and 27 shared among all three conditions through databases such as SwissTargetPrediction and GeneCards.