A single-molecule biosensor based on multiple fluorophore nucleic acid probe and STORM imaging for detection of Hantaan virus.

Hemorrhagic fever with renal syndrome (HFRS), caused by Hantaan virus, poses a serious public health threat. Current diagnostic methods remain limited by low sensitivity, complex procedures, and high sample requirements. To address this, we developed a highly sensitive single-molecule biosensor using multi-fluorophore nucleic acid probes and STORM imaging for the detection of Hantaan virus RNA.

Lindenane-Based Sesquiterpenoid Hetero-Oligomers with Diverse Skeletons and Extracellular Regulated Protein Kinases Inhibitory Activity from .

Sarcglabtenes A-G (-), seven lindenane-based sesquiterpenoid hetero-oligomers with six unprecedented skeletons, along with five new biosynthetic analogues sarcglabtenes H-L (-), were isolated from . Their structures including absolute configurations were comprehensively elucidated using HR-MS, NMR, ECD, and single crystal X-ray diffraction. Structurally, sarcglabtenes A-G are lindenane hetero-oligomers including a geranyl homogentisic acid (/), geranylgeranyl -toluquinone (/), germarane (), campholenal (/) derivatives, for which plausible biosynthesis pathways are also proposed.

State-specific thalamic dysregulation in chronic insomnia revealed by dynamic edge-centric functional connectivity.

To investigate dynamic cortical-thalamocortical-subcortical network dysregulation in chronic insomnia (CI) using dynamic edge functional connectivity (deFC) analysis, focusing on thalamus-mediated transient connectivity states and their clinical correlations. 30 CI patients and 32 matched healthy controls (HCs) underwent resting-state fMRI. We calculated deFC with the thalamus as a hub node, identified transient connectivity states via K-means clustering, and compared inter-group differences.

Smart supply chain adoption and urban energy transition: Empirical evidence on de-coalization effects.

Coal has occupied a high position in the energy consumption structure of Chinese cities, which has become an urgent challenge. A smart supply chain, as an innovative tool for the deep integration of the real economy and digital technology, provides new ideas for optimizing energy consumption structure. Based on data from Chinese prefecture-level cities from 2012 to 2022, we consider supply chain innovation and pilot work as a quasi-natural experiment and systematically examine the impact of smart supply chains on urban energy consumption structure and its mechanism of action.

Study on axial compressive behavior and mesoscopic structural evolution of FRP confined coal gangue concrete.

To address the environmental hazards caused by coal gangue waste, coal gangue concrete (CGC) has been proposed as a solution. However, due to its porosity and low strength, CGC faces numerous challenges in practical applications. To further improve its performance, fiber reinforced polymer (FRP) material was introduced to confine it.

N6-methyladenosine modification of the subgroup J avian leukosis viral RNAs attenuates host innate immunity via MDA5 signaling.

Subgroup J avian leukosis virus (ALV-J), a retrovirus, elicits immunosuppression and persistent infections in chickens. Although it is widely acknowledged that ALV-J can evade the host's innate immune defenses, the mechanisms behind this immune evasion remain elusive. N6-methyladenosine (m6A), the most prevalent internal RNA modification, plays a role in innate immune evasion.

[Effects of Organic Materials Applied to Saline Soil on Crop Growth and Soil Saline-alkali Index].

To provide a theoretical basis for the scientific application of organic materials in salinized soil and the improvement of soil quality and to explore the response phenotype of an organic material-salinized soil-plant system, the salinized soil in Xinjiang was considered as the research object, and cotton, rape, and oil sunflower were considered as the test crops. Six treatments, including the control group, microbial agent, humic acid, organic fertilizer, biochar, and straw, were set up for indoor culture experiments. The effects of different organic materials on the growth of cotton, oil sunflower, and rape crops and soil saline-alkali indexes under moderate salinization soil conditions were analyzed, and the combinations with certain improvement effects were screened out.

Diverse anti-inflammatory lindenane sesquiterpenoid hetero- or homo-dimers from Chloranthus holostegius var. shimianensis.

Twenty-seven dimeric lindenane sesquiterpenoid (LS) derivatives, including thirteen new hetero- or homo-dimers (1-13), were discovered from rare Chloranthaceae plant Chloranthus holostegius var. shimianensis. Their structures were elucidated by a combination of HRMS, spectroscopic analysis, single-crystal X-ray diffraction and CD exciton chirality method.

Associations of ischemic heart disease with brain glymphatic MRI indices and risk of Alzheimer's disease.

Background: The impact of ischemic heart disease (IHD) on the brain glymphatic MRI indices and risk of Alzheimer's disease (AD) remains largely unclear. This study aimed to investigate the associations between IHD, brain glymphatic MRI indices and risk of AD.

Methods: A total of 1385 non-dementia subjects (55.

Recombinant Marek's disease virus expressing VP1 and VP2 proteins provides robust immune protection against chicken infectious anemia virus.

Chicken infectious anemia (CIA) is a highly contagious disease caused by the chicken infectious anemia virus (CIAV), and it poses a serious threat to the poultry industry. However, effective control measures and strategies have not been identified. In this study, a recombinant Marek's disease virus (rMDV) expressing the VP1 and VP2 proteins of CIAV was successfully constructed using CRISPR/Cas9, and a commercial Marek's disease virus (MDV) vaccine strain was used as the vector.

Structurally diverse sesquiterpenoids from Sarcandra glabra and their anti-inflammatory activities.

Fifteen sesquiterpenoids, including five previously undescribed monomers with oxidative rearranged skeletons (sarglabenoids A-E, 1-5) and three previously unreported lindenane [2 + 2] dimers (sarglabenoids F-H, 6-8), alongside seven related precursors (9-15), were isolated from the root of Sarcandra glabra. The structures of these compounds were elucidated using a combination of high-resolution electrospray ionization mass spectrometry, one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy, the circular dichroism exciton chirality method, electronic circular dichroism, and nuclear magnetic resonance calculations integrated with DP4+ analysis. Compounds 1 and 2 feature an unique 5/5 spiro ring system, which is likely derived from a pinacol rearrangement of precursor 14.

Human ANP32A/B are SUMOylated and utilized by avian influenza virus NS2 protein to overcome species-specific restriction.

Human ANP32A/B (huANP32A/B) poorly support the polymerase activity of avian influenza viruses (AIVs), thereby limiting interspecies transmission of AIVs from birds to humans. The SUMO-interacting motif (SIM) within NS2 promotes the adaptation of AIV polymerase to huANP32A/B via a yet undisclosed mechanism. Here we show that huANP32A/B are SUMOylated by the E3 SUMO ligase PIAS2α, and deSUMOylated by SENP1.

The Impact of MAFLD on Coronary Plaque Characteristics and Physiologic Status: A Coronary CT Angiography Study.

Rationale And Objectives: Metabolic dysfunction-associated fatty liver disease (MAFLD) is linked to an increased risk of cardiovascular events. Our study sought to determine the impact of MAFLD on both the anatomy and function of coronary plaques.

Materials And Methods: A total of 203 participants (including 728 plaques) with suspected coronary artery disease (CAD) who underwent coronary CT angiography (CCTA) and abdominal ultrasound were prospectively enrolled.

Equine ANP32 proteins support influenza A virus RNA polymerase activity.

Host ANP32 family proteins are crucial for maintaining the activity of influenza RNA polymerase and play an important role in the cross-species transmission of influenza viruses. To date, the molecular properties of equine ANP32 (eqANP32) protein are poorly understood, particularly the mechanisms that affect equine influenza virus (EIV) RNA polymerase activity. Here, we found that there are six alternative splicing variants of equine ANP32A (eqANP32A) with different levels of expression.

Energy dissipation damage constitutive relation of CFRP passively confined coal sample.

In view of the stability problem of coal pillars left over during coal resource mining, (Carbon Fiber Reinforced Polymer) CFRP sheet is applied in coal pillar reinforcement. Uniaxial compression tests of CFRP passively confined coal samples are carried out to explore the mechanical response mechanism of passively confined coal samples under different layers, and the energy dissipation damage constitutive relationship of CFRP passively confined coal samples is established based on the energy dissipation principle. The conclusions are: As CFRP layers increased, the local damage of coal samples before the peak evolved from a 'cliff-like jagged' to a 'capillary jagged', with post-peak instability marked by a shift to more 'cliff-like' characteristics.

Discovery of Quality by Design-Based Extraction Technology and the Quality Standard of Jinhua Finger Citron Essential Oil.

Jinhua finger citron is one of the traditional specialties of Jinhua with a long history of cultivation. The current study highlighted the advantages of using the quality by design approach to optimize the ultrasonic-assisted distillation extraction of Jinhua finger citron essential oil. The yield of Jinhua finger citron essential oil was regarded as a potential critical quality attribute.

Identification of Cables1 as a critical host factor that promotes ALV-J replication via genome-wide CRISPR/Cas9 gene knockout screening.

Avian leukosis virus subgroup J (ALV-J), a member of the genus Alpharetrovirus, possesses a small genome and exploits a vast array of host factors during its replication cycle. To identify host factors required for ALV-J replication and potentially guide the development of key therapeutic targets for ALV-J prevention, we employed a chicken genome-wide CRISPR/Cas9 knockout library to screen host factors involved in ALV-J infection within DF-1 cells. This screening revealed 42 host factors critical for ALV-J infection.

Corneal stromal structure replicating humanized hydrogel patch for sutureless repair of deep anterior-corneal defect.

A critical shortage of donor corneas exists worldwide. Hydrogel patches with a biological architecture and functions that simulate those of native corneas have garnered considerable attention. This study introduces a stromal structure replicating corneal patch (SRCP) composed of a decellularized cornea-templated nanotubular skeleton, recombinant human collagen, and methacrylated gelatin, exhibiting a similar ultrastructure and transmittance (above 80 %) to natural cornea.

Cryo-EM structures of Thogoto virus polymerase reveal unique RNA transcription and replication mechanisms among orthomyxoviruses.

Influenza viruses and thogotoviruses account for most recognized orthomyxoviruses. Thogotoviruses, exemplified by Thogoto virus (THOV), are capable of infecting humans using ticks as vectors. THOV transcribes mRNA without the extraneous 5' end sequences derived from cap-snatching in influenza virus mRNA.

Association of Lipoprotein(a) Levels With Myocardial Infarction in Patients With Low-Attenuation Plaque.

Background: Lipoprotein(a) (Lp[a]) is associated with an increased risk of myocardial infarction (MI). However, the mechanism underlying this association has yet to be fully elucidated.

Objectives: This multicenter study aimed to investigate whether association between Lp(a) and MI risk is reinforced by the presence of low-attenuation plaque (LAP) identified by coronary computed tomography angiography (CCTA).

Overexpression of enhances freezing tolerance by increasing the expression of related genes in the .

Mitogen-activated protein kinases (MAPKs) play important roles in plant stress response. As a major member of the MAPK family, MPK3 has been reported to participate in the regulation of chilling stress. However, the regulatory function of wheat (Triticum aestivum ) mitogen-activated protein kinase TaMPK3 in freezing tolerance remains unknown.

OASL suppresses infectious bursal disease virus replication by targeting VP2 for degrading through the autophagy pathway.

Unlabelled: Infectious bursal disease (IBD) is an acute and fatal immunosuppressive disease caused by infectious bursal disease virus (IBDV). As an obligate intracellular parasite, IBDV infection is strictly regulated by host factors. Knowledge on the antiviral activity and possible mechanism of host factors might provide the theoretical basis for the prevention and control of IBD.

N123I mutation in the ALV-J receptor-binding domain region enhances viral replication ability by increasing the binding affinity with chNHE1.

The subgroup J avian leukosis virus (ALV-J), a retrovirus, uses its gp85 protein to bind to the receptor, the chicken sodium hydrogen exchanger isoform 1 (chNHE1), facilitating viral invasion. ALV-J is the main epidemic subgroup and shows noteworthy mutations within the receptor-binding domain (RBD) region of gp85, especially in ALV-J layer strains in China. However, the implications of these mutations on viral replication and transmission remain elusive.

Avian ANP32A incorporated in avian influenza A virions promotes interspecies transmission by priming early viral replication in mammals.

Species-specific differences in acidic nuclear phosphoprotein 32 family member A (ANP32A) determine the restriction of avian-signature polymerase in mammalian cells. Mutations that evade this restriction, such as PB2-E627K, are frequently acquired when avian influenza A viruses jump from avian hosts to mammalian hosts. However, the mechanism underlying this adaptation process is still unclear.