Neuro-Immuno-Stromal Context in Colorectal Cancer: An Enteric Glial Cell-Driven Prognostic Model via Machine Learning Predicts Survival, Recurrence, and Therapy Response.

Background: Enteric glial cells (EGCs) have been implicated in colorectal cancer (CRC) progression. This study aimed to develop and validate a prognostic model integrating EGC- and CRC-associated gene expression to predict patient survival, recurrence, metastasis, and therapy response.

Methods: Bulk and single-cell RNA sequencing data were analyzed, and a machine learning-based model was constructed using the RSF random forest algorithm.

Endogenous YAP/TAZ partitioning in TEAD condensates orchestrates the Hippo response.

YAP/TAZ are transcriptional co-activators that pair with transcription factor TEA/ATTS domains (TEADs) for modulating the Hippo pathway. Previous works propose the potential role of YAP/TAZ phase separation for transcriptional activation, yet the biomolecular basis of endogenous YAP/TAZ-TEAD condensates remains unclear. Here, we dissect their endogenous morphology, revealing that YAP/TAZ are client proteins recruited to TEAD condensates in various human cell lines.

Elucidating the correlation between polychlorinated dibenzo-p-dioxins and prostate cancer progression: Insights from gene expression and molecular docking.

Objective: Polychlorinated dibenzo-p-dioxins (PCDDs) present a significant long-term threat to human health attributable to their toxicological properties, chemical stability and propensity for bioaccumulation. This study seeks to explore the correlation between PCDDs exposure and prostate cancer (PCa) through comprehensive analysis.

Methods: The multi-dimensional analysis was conducted based on various online databases.

Metallothionein 2A-Silencing Antimonene-Based Nanoagonist for Amplifying the Photothermal Immunotherapy of Gynecologic Malignancy.

Gynecologic malignancies are prone to metastasis and recurrence due to the low efficacy and sensitivity of current clinical treatments. Here, we construct ultrasmall Sb@Au nanodots (Sb@Au NDs) as a metallothionein 2A (MT 2A)-silencing nanoagonist for effective photothermal immunotherapy of gynecologic malignancies. Sb@Au NDs show high photothermal conversion efficiency of 56.

Hyperactivity of subicular parvalbumin interneurons drives early amyloid pathology and cognitive deficits in Alzheimer's disease.

Atrophy of the subiculum is the earliest hippocampal anatomical marker of Alzheimer's disease (AD) and is closely associated with early cognitive decline. However, the underlying mechanisms driving this vulnerability remain unclear. In this study, using the 5×FAD mouse model, we identified significant amyloid-beta (Aβ) accumulation in the subiculum during the early stages of AD.

A Cascaded Segmentation-Classification Deep Learning Framework for Preoperative Prediction of Occult Peritoneal Metastasis and Early Recurrence in Advanced Gastric Cancer.

Purpose: To develop a cascaded deep learning (DL) framework integrating tumor segmentation with metastatic risk stratification for preoperative prediction of occult peritoneal metastasis (OPM) in advanced gastric cancer (GC), and validate its generalizability for early peritoneal recurrence (PR) prediction.

Methods: This multicenter study enrolled 765 patients with advanced GC from three institutions. We developed a two-stage framework as follows: (1) V-Net-based tumor segmentation on CT; (2) DL-based metastatic risk classification using segmented tumor regions.

Lactococcus formosensis and its metabolite 4-acetamidobutanoic acid induced caspase-11 dependent myenteric neuronal pyroptosis in intractable functional constipation.

Intractable functional constipation (IFC), a severe form of chronic constipation characterized by slow transit and resistance to conventional treatments, posed a significant clinical challenge. Here, we identified Lactococcus formosensis (Lf), a Gram-positive bacterium prevalent in IFC patients, as a novel contributor to intestinal motility impairment. Clinically, IFC patients exhibited increased colonic mucosal colonization of Lf and significant myenteric neuronal loss and pyroptosis, particularly in excitatory choline acetyltransferase (ChAT) neurons, but not inhibitory neuronal nitric oxide synthase (nNOS) neurons.

Impact of rare EGFR exon 19 insertion-deletion variants on tyrosine kinase inhibitor efficacy in non-small-cell lung cancer: a 5-year multicenter analysis.

Background: The clinical impact of rare epidermal growth factor receptor (EGFR) exon 19 insertion-deletion (19delins) variants on tyrosine kinase inhibitor (TKI) efficacy remains poorly characterized. We updated 5-year outcomes to evaluate long-term survival and optimal treatment strategies in advanced lung adenocarcinoma (LUAD) patients harboring these mutations.

Materials And Methods: In this multicenter prospective study, 36 treatment-naive advanced LUAD patients with EGFR 19delins mutations received first-generation (n = 26) or third-generation TKIs (n = 10).

Surgical resection of Wernicke's area brain arteriovenous malformation via posterior Sylvian fissure: Two-dimensional video.

The brain arteriovenous malformation (BAVM) within language-eloquent area poses a significant surgical challenge, demanding meticulous planning to ensure both preservation of language function and curative resection. This report details the successful microsurgical resection of a Spetzler-Martin grade II BAVM located in Wernicke's area in a 51-year-old male, ruptured three weeks ago and characterized by mild anomia. Following thorough discussion, the patient elected for microsurgery, providing informed consent, and the procedure was approved by the ethics committee.

Effects of Liver Enzyme Levels During Pregnancy on Birth Weight.

Objectives: To investigate the association between maternal liver enzyme concentrations during pregnancy and the risk of abnormal birth weight.

Methods: This is a prospective birth cohort study querying the pregnant women from Fujian Maternal and Child Health Hospital, affiliated with Fujian Medical University, China. Liver enzyme levels, including gamma-glutamyl transferase (GGT), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), were measured in the first and third trimesters, and changes in liver enzyme levels were calculated based on these measurements.

HBV-encoded circRNA-5 promotes hepatocellular carcinoma progression by regulating the miR-9-3p/Hippo signaling axis.

Chronic hepatitis B virus (HBV) infection is regarded as one of the most serious infectious diseases and a significant global public health concern. Although the neonatal vaccine has been effective in impeding the transmission of HBV, tens of millions of HBV patients are still vulnerable to liver disease and even hepatocellular carcinoma (HCC). In this research, we demonstrated that HBV-encoded circRNA, designated as HBV-circRNA-5, was involved in the tumorigenesis of HCC.

Comparison of angiographically derived coronary radial wall strain and superficial wall stress for the characterization of plaque vulnerability.

Background: Angiography-derived radial wall strain (RWS) estimates the radial coronary wall deformation caused by pulsatile blood pressure, whereas superficial wall stress (SWS) summarizes the comprehensive wall deformation caused by both blood pressure and cardiac motion. This study sought to investigate the difference between RWS and SWS for the association with plaque vulnerability and the impact of cardiac motion on RWS.

Methods: Concurrent RWS, cardiac motion-induced bending angle change (ΔCBA), SWS, and optical coherence tomography image analyses were retrospectively performed in 49 eligible intermediate coronary lesions.

The Global, Regional, and National Burden of Inflammatory Bowel Disease among Children and Adolescents from 1990 to 2021 and Trend Projections up to 2036.

Background/aims: Early-onset inflammatory bowel disease (EO-IBD) poses a global health challenge with its distinct clinical manifestations and complex progression.

Methods: In this study, IBD cases occurring before age 20 were defined as EO-IBD. Data were extracted from the Global Burden of Disease 2021 database.

Epidemiological trends of early-onset gastrointestinal cancers from 1990 to 2021 and predictions for 2036: analysis from the global burden of disease study 2021.

Background: Gastrointestinal cancers account for 39.0% of global cancer-related deaths. The rising incidence of early-onset gastrointestinal cancers poses a substantial public health challenge due to their aggressive nature and poor prognosis.

Research on the changes in the disease burden of nasopharyngeal carcinoma caused by global occupational formaldehyde exposure from 1990 to 2021 and prediction of future trends.

Background: Explore the changes in the burden of nasopharyngeal carcinoma (NPC) caused by occupational formaldehyde exposure from 1990 to 2021, and predict its development trend up to 2050, to provide references for formulating relevant policies and measures.

Methods: Using the Global Burden of Disease database 2021 (GBD 2021), we counted and analyzed the numbers and standardized rates of NPC deaths and disability-adjusted life years (DALYs) caused by occupational formaldehyde exposure globally, in 21 regions, and in 204 countries. Combined with correlation analysis, health inequality analysis, and frontier analysis, we further comprehensively described the disease burden and its changing trends.

Parents' understanding and attitudes toward the application of AI in pediatric healthcare: a cross-sectional survey study.

Objective: To investigate parents' understanding and attitudes toward the application of Artificial Intelligence (AI) in pediatric healthcare.

Methods: An observational, cross-sectional study was conducted using an questionnaire. Between February and April 2025, 200 family members of children receiving care at our hospital voluntarily participated in the study.

Prognostic value of the log odds of negative lymph nodes/T stage ratio (LONT) in postoperative esophageal cancer: a SEER-based study.

Introduction: Surgery remains the primary treatment for patients with esophageal cancer (EC), yet postoperative prognosis is often unsatisfactory. Accurate prediction of cancer-specific survival (CSS) can assist clinicians in personalized treatment planning. This study aimed to develop an interactive web-based tool to estimate CSS in patients with T1~3N0~2M0 EC after surgery, based on the log odds of negative lymph nodes/T stage ratio (LONT).

Genetic etiology and pregnancy outcomes of fetal hyperechoic kidneys: a retrospective analysis.

Background: Fetal hyperechoic kidney is an important soft marker in prenatal ultrasonography; however, the causes of this phenomenon are unclear. Therefore, we analyzed genetic diagnosis results, assessed pregnancy outcomes, and conducted postnatal follow-up to provide evidence for prenatal eugenics.

Methods: We retrospectively analyzed data from 94 cases with fetal hyperechoic kidneys identified between November 2017 and January 2024.

Analyzing the correlation between the longitudinal trajectories of blood urea nitrogen and 30-day survival in hemorrhagic stroke patients.

Background: While some studies suggest a link between blood urea nitrogen (BUN) levels and adverse outcomes in hemorrhagic stroke (HS) patients, the prognostic value of longitudinal BUN changes remains unclear.

Objective: To evaluate the association between longitudinal BUN trajectories and 30-day mortality risk in HS patients.

Methods: We analyzed HS patients from the MIMIC-IV database diagnosed within 24 hours of hospitalization.

Construction and Validation of a Novel Prognostic Model Based on Cervical Cancer-Related Genes.

Background: Cervical cancer (CC) is the fourth most frequently diagnosed cancer and the fourth leading cause of cancer-related deaths in women worldwide, however, the treatment options for advanced CC are limited. Therefore, there is an urgent need in the clinic for reliable prognostic models to guide clinical decision-making.

Methods: We conducted differential gene expression analysis on cervical cancer samples and normal samples to obtain differentially expressed genes (DEGs).