Genome-wide association study reveals novel SNP loci and candidate genes linked to flowering time in upland cotton.

A total of 456 SNPs associated significantly with FT via GWAS and three candidate genes related to flowering were identified via RNA‒seq, qRT‒PCR and VIGS. Flowering time (FT) is one of the main traits associated with early maturity in upland cotton; however, genetic basis and candidate genes underlying FT remain inadequately understood. In this study, 1,574,032 high-quality single nucleotide polymorphisms (SNPs) were identified on the basis of resequencing data from 619 upland cotton lines, and among them, 418 core germplasms were selected and genome-wide association studies (GWASs) were conducted to identify 456 SNPs that were significantly associated with FT.

Integrated analysis of miRNAs-mRNAs in skeletal muscle development revealed that novel-miR-766 affects myoblast differentiation and myofiber-type formation in sheep.

Background: Various regulators coregulate muscle development in animals. MicroRNAs (miRNAs) are crucial regulators that participate in multiple aspects of myofiber formation. Method: To identify key miRNAs and target genes associated with muscle development, embryos or longissimus dorsi of Chinese Merino sheep were collected for whole-transcriptome sequencing across 11 gestation periods: 26 days (D26), D29, D32, D35, D40, D45, D55, D75, D85, D105, and D135.

-Glycerol monolaurate promotes tight junction proteins expression through PKC/MAPK/ATF-2 signaling pathway.

Introduction: This study investigates the effects of -GML on intestinal epithelial tight junction (TJ) protein expression and its molecular mechanisms. Recognizing the critical role of TJ proteins in intestinal barrier function and the potential of -GML to enhance this barrier, we employ the IPEC-J2 cell model. Our aim is to validate the regulatory impact ofα-GML on TJ protein expression and elucidate the underlying signaling pathways, thereby offering new strategies for intestinal health maintenance.

Transcriptomic profiling reveals SARS-CoV-2-infected humanized MHC mice recapitulate human post vaccination immune responses.

Background: The ongoing COVID-19 pandemic caused by SARS-CoV-2 remains a critical global health priority, with persistent socioeconomic ramifications despite its reclassification from Public Health Emergency of International Concern (PHEIC) status. While humanized major histocompatibility complex (hMHC) murine models have been extensively utilized in oncological research, their application in virological studies-particularly for coronavirus pathogenesis-remains underexplored.

Methods: This study systematically characterized immune responses in SARS-CoV-2-challenged hMHC mice lung tissues through comparative transcriptomic profiling, combined with functional enrichment and PPI network analyses.

Antitumor power of oncolytic HSV engineered with IL-12 and IL-15.

Oncolytic herpes simplex virus type 1 (HSV-1) directly targets and destroys tumor cells while selectively infecting surrounding tumor tissue and releasing progeny viruses to continue the attack. We engineered the replicative HSV-1 vector, including the oncolytic HSV-1 (oHSV) with deleted inverted repeat and α47 gene, and then the oHSV expressing interleukin-15 (IL-15) gene and the oHSV expressing IL-15 and interleukin-12 (IL-12) gene. We evaluated the titer and growth characteristics of the oHSV-1 in Vero and cancer cell lines.

Bioinspired Anti-Freezing Hydrogel With Localized Ice Regulation for Subzero Soft Robotics.

Freezing hydrogels at subzero temperatures severely compromises mechanical flexibility, ionic conductivity, and structural integrity, thereby limiting their application in low-temperature environments. Hydrogel freezing involves both ice nucleation and ice growth; however, simultaneously inhibiting these two processes remains a significant challenge. In nature, freeze-tolerant organisms do not rely on completely preventing ice formation to survive freezing conditions.

Rapamycin coated selenium nanoparticles relieve oxidative senescence of vascular endothelium by mitophagy.

Rapamycin (RPM) extends longevity in various species and combats vascular senescence related diseases. Selenium nanoparticles (SeNPs) have attracted attention as a potential therapy for cardiovascular diseases due to their excellent antioxidant and drug-carrying capacity. However, RPM coated SeNPs (RPM-SeNPs) have not been reported and their potential for preventing endothelial oxidative senescence remains unclear.

Pangenome analysis of transposable element insertion polymorphisms reveals features underlying cold tolerance in rice.

Transposable elements (TEs) introduce genetic and epigenetic variability, contributing to gene expression patterns that drive adaptive evolution in plants. Here, we investigate TE architecture and its effect on cold tolerance in rice. By analyzing a pangenome graph and the resequencing data of 165 rice accessions, we identify 30,316 transposable element insertion polymorphism (TIP) sites, highlighting significant diversity among polymorphic TEs (pTEs).

Targeted elimination of senescent cells by ProcyanidinC1 improves diabetic wound healing and restores skin quality.

For diabetic patients, impaired wound healing is a serious complication, which characterized by prolonged inflammation, wound granulation tissue formation obstruction and impaired re-epithelialization. Accumulating evidence shows that senescent cells play a crucial role in the pathomechanism of diabetic wounds. In this study, we systematically evaluated the role of senescent cells in diabetic wound healing through diabetic mice (DM mice) model (including streptozotocin-induced type I DM mice model and db/db (type II DM) mice model, and actively assessed the therapeutic potential of ProcyanidinC1 (PCC1), the novel senolytic compound.

Microcystin-LR hijacks mitochondrial bioenergetics and Wnt crosstalk: Unveiling a novel dual-pathway mechanism for adipogenesis dysregulation in environmental toxicology.

Cyanobacterial blooms, driven by toxin-producing cyanobacteria, pose significant environmental health risks due to the persistent release of cyanotoxins like microcystin-LR (MC-LR). While much research has focused on its hepatotoxic, nephrotoxic, and neurotoxic effects, its impact on adipose tissue homeostasis remains poorly understood. This study investigates the effects of MC-LR on stromal vascular fraction (SVF) cells, key progenitors in adipogenesis and metabolic regulation.

Interplay mechanism of virus latency-reactivation by human cytomegalovirus UL82 protein with the host core histone H3K27 methyltransferase EZH2 of polycomb repressive complex 2.

Early studies have shown that epigenetic modifications play an important role in the establishment of latent human cytomegalovirus (HCMV) infection. However, the specific regulatory mechanisms remain unclear, especially regarding how HCMV overcomes these silencing effects of epigenetic modifications during viral reactivation from latency. Here, we showed that HCMV reactivation from latency is indeed regulated by histone H3K27 trimethylation.

Aerobic granular sludge: Formation mechanism, accelerating granulation strategies, and emerging applications.

Industry, pharmaceuticals and catering all generate large amounts of complex wastewater that urgently requires effective treatment. Aerobic Granular Sludge (AGS) has emerged as a transformative technology in sustainable wastewater treatment, characterized by its compact structure, high biomass retention and multiple pollutants removal capability. The unique spatial layered structure allows AGS to simultaneously remove COD, nitrogen, phosphorus and other pollutants from wastewater in a single reactor.

Pectin/arabinoxylan dietary polysaccharide microcapsule encapsulated with Clostridium butyricum for targeted delivery in colitis treatment.

The gut microbiota plays a critical role in maintaining intestinal homeostasis, and its dysbiosis is a key factor in the development of inflammatory bowel diseases (IBD), such as colitis. This study developed an innovative dual-layer synbiotic microcapsule system for targeted delivery of Clostridium butyricum (C. butyricum) to treat dextran sulfate sodium (DSS) induced colitis.

Matrix regulation: a plug-and-tune method for combinatorial regulation in Saccharomyces cerevisiae.

Transcriptional fine-tuning of long pathways is complex, even in the extensively applied cell factory Saccharomyces cerevisiae. Here, we present Matrix Regulation (MR), a CRISPR-mediated pathway fine-tuning method enabling the construction of 6 gRNA combinations and screening for the optimal expression levels across up to eight genes. We first identify multiple tRNAs with efficient gRNA processing capacities to assemble a gRNA regulatory matrix combinatorially.

Smart bacteriophage release from bovine β-lactoglobulin fibrils based hydrogel with anti-MRSA, biofilm degradation and hair follicle regeneration functions for infected burn wound.

The emergence and spread of antibiotic-resistant bacteria negatively impact the effectiveness of antibiotics in treating burn wound infections, which significantly hinders the healing process. To address this, a β-lactoglobulin fibrils/oxidized dextran/phage (BLGFs/ODEX/Phage) hydrogel dressing has been developed to treat burn wounds that are infected with methicillin-resistant Staphylococcus aureus (MRSA). First, a highly efficacious MRSA phage is isolated and purified, and it exhibits excellent bactericidal efficiency, storage stability, biofilm degradation ability, and biocompatibility.

Toti-N-glycan Recognition Enables Universal Multiplexed Single-Nucleus RNA Sequencing.

Sample barcoding-based multiplex single-cell and single-nucleus sequencing (sc/sn-seq) offers substantial advantages by reducing costs, minimizing batch effects, and identifying artifacts, thereby advancing biological and biomedical research. Despite these benefits, universal sample barcoding has been hindered by challenges such as inhomogeneous expression of tagged biomolecules, limited tagging affinity, and insufficient genetic insertion. To overcome these limitations, we developed Toti-N-Seq, a universal sample multiplex method, by tagging Toti-N-glycan on cell surfaces or nuclear membranes via our engineered streptavidin-Fbs1 GYR variant fusion protein, which could be used not only for sc-seq but also for sn-seq.

Glyco-engineered multifunctional hydrogel with bacterial fishing hooks for targeted bacterial eradication and enhanced wound healing.

Infection with drug-resistant bacteria in hospitals significantly hinders wound healing, resulting in a high mortality rate among affected patients. In this study, we developed a multifunctional dextran-based hydrogel platform that effectively captures and eliminates carbapenem-resistant (CRPA) from wounds. By grafting glycoligands (galactose and fucose) onto the three-dimensional hydrogel matrix and two-dimensional black phosphorus nanosheets, we substantially enhance the selective capture of CRPA through multivalent carbohydrate-lectin interactions.

Mango pangenome reveals dramatic impacts of reference bias on population genomic analyses.

Most genomic studies start by mapping sequencing data to a reference genome. The quality of reference genome assembly, genetic relatedness to the studied population, and the mapping method employed directly impact variant calling accuracy and subsequent genomic analyses, introducing reference bias and resulting in erroneous conclusions. However, the impacts of reference bias have gained limited attention.

Characterization and Complete Genomic Analysis of a Novel Bacteriophage BUCT775 for and Its Elimination Efficiency in the Environment.

() is an opportunistic pathogen responsible for a range of severe infections and nosocomial outbreaks. Phage-based therapy and biocontrol represent effective strategies to combat the prevalence of . This study reports a novel phage, BUCT775, capable of specifically lysing , and investigates its physiological properties, genomic characteristics, in vivo therapeutic efficacy, and environmental disinfection performance.

Optimization of Chromosome Preparation and Karyotype Analysis of Winter Turnip Rape ( L.).

To explore the dyeing technique and karyotype analysis of winter turnip rape ( L.), the root tip of winter turnip rape Longyou 7 was used as the experimental material. Chromosome preparation technology was optimized, and karyotype analysis was carried out by changing the conditions of material collection time, pretreatment, fixation, and dissociation.

Comparative Analysis of Chloroplast Genome Between Widely Distributed and Locally Distributed (Gesneriaceae) Related Members.

The genus belongs to the family Gesneriaceae and includes plants with both ornamental and medicinal value. However, genomic-level data on the genus remains scarce. Previous investigations of have predominantly centered on morphological classification, with only limited exploration of molecular phylogenetics.

Screening and Application of Highly Efficient Rhizobia for Leguminous Green Manure in Lyophilized Inoculants and Seed Coating.

, a key leguminous green manure widely cultivated in Southern China's rice-based cropping systems, plays a pivotal role in sustainable agriculture by enhancing soil organic matter sequestration, improving rice yield, and elevating grain quality. The symbiotic nitrogen-fixing association between and its matching rhizobia is fundamental to its agronomic value; however, suboptimal inoculant efficiency and field application methodologies constrain its full potential. To address these limitations, we conducted a multi-phase study involving (1) rhizobial strain screening under controlled greenhouse conditions, (2) an optimized lyophilization protocol evaluating cryoprotectant (trehalose, skimmed milk powder and others), and (3) seed pelleting trails with rhizobial viability and nodulation assessments over different storage periods.

Establishment of -Mediated Transient Transformation System in Sunflower.

Sunflower ( L.) is an important oilseed crop in Northwest China, exhibiting resistance to salt and drought. Mining its excellent tolerance genes can be used for breeding.

Genome-Wide Identification of DNA Methyltransferase and Demethylase in sect. Turanga and Their Potential Roles in Heteromorphic Leaf Development in .

DNA methylation, mediated by DNA methyltransferases (DMTs) and demethylases (DMLs), is an important epigenetic modification that maintains genomic stability and regulates gene expression in plant growth, development, and stress responses. However, a comprehensive characterization of these gene families in sect. Turanga remains lacking.

Transcriptome Analysis and Functional Characterization of the Gene Involved in Barley Resistance to .

Barley leaf stripe, caused by (), significantly reduces yields across various regions globally. Understanding the resistance mechanisms of barley to is crucial for advancing disease resistance breeding efforts. In this study, two barley genotypes-highly susceptible Alexis and immune Ganpi2-were inoculated with the highly pathogenic isolate QWC for 7, 14, and 18 days.