Post-Marketing Safety Surveillance of HPV Vaccines in Anhui Province, China, 2017-2024.

The Human Papillomavirus (HPV) vaccine has been globally implemented to prevent HPV-associated diseases. Since its introduction in China in 2017, this vaccine has substantially reduced the burden of HPV-related health conditions. As more people get vaccinated these days, ongoing safety monitoring and evaluation have become critical.

Mango pangenome reveals dramatic impacts of reference bias on population genomic analyses.

Most genomic studies start by mapping sequencing data to a reference genome. The quality of reference genome assembly, genetic relatedness to the studied population, and the mapping method employed directly impact variant calling accuracy and subsequent genomic analyses, introducing reference bias and resulting in erroneous conclusions. However, the impacts of reference bias have gained limited attention.

Comparative Genomics and Phylogenetics of Chloroplasts Reveal Lower Rates of Genetic Variation in Mango ().

The mango is an important economic crop with a long history of cultivation. However, studies on the characteristics among chloroplast (cp) genomes and the phylogenetic relationships of different mango varieties are still limited. To fill this research gap, we assembled, annotated, and compared the cp genomes of 23 mango germplasms.

Positioning of Chinese time nouns and adverbs: Evidence from corpus, acceptability, and processing studies.

This study examines the syntactic placement and cognitive processing of time nouns and time adverbs in Mandarin Chinese, a language without overt tense morphology, highlighting how these temporal expressions interface with Chinese grammar. Study 1 analyzed a large-scale natural language corpus (BLCU Chinese Corpus) to determine the typical positions of time nouns and time adverbs relative to the subject. The results revealed distinct distributional patterns: time nouns occurred flexibly either before or after the subject, while time adverbs appeared predominantly in post-subject (sentence-internal) positions.

RXR modulates hepatic stellate cell activation and liver fibrosis by targeting CaMKK-AMPK axis.

Hepatic stellate cells (HSCs) are the primary fibrogenic cells in the liver, and their activation plays a crucial role in the development and progression of hepatic fibrosis. Here, we report that retinoid X receptor-alpha (RXR), a unique member of the nuclear receptor superfamily, is a key modulator of HSC activation and liver fibrosis. RXR exerts its effects by modulating calcium/calmodulin-dependent protein kinase kinase (CaMKK)-mediated activation of AMP-activated protein kinase-alpha (AMPK).

Clinical and Genetic Characteristics of Patients with Early-Onset Diabetes Involving at Least Two Consecutive Generations: Whole-Exome Sequencing in Probands from 25 Pedigrees.

Background: The molecular mechanisms of early-onset multigenerational diabetes remain unknown. This study aimed to investigate the clinical and genetic characteristics of early-onset diabetes involving at least two consecutive generations.

Methods: From 1296 inpatients with diabetes, we selected individuals who were ≤ 30 years of age and who were clinically suspected of having familial monogenic diabetes.

The Quest for the Locus.

Unlabelled: Genetic gain in potato breeding is limited by the heterozygous tetraploid genome of cultivated potato. Recent efforts to breed potato at the diploid level promise to improve genetic gain and allow more straightforward genetics and introgression breeding. Diploid F1 hybrid potato breeding relies on the ability to create diploid inbred lines via repeated self-fertilization.

C/EBP-α Promotes Mitochondrial Fission and Inhibits the Activation of Hepatic Stellate Cells via YAP-Drp1 Pathway.

The activation of hepatic stellate cells (HSCs) plays a key role in the pathogenesis of liver fibrosis. However, the activation of HSCs requires energy from mitochondria-highly dynamic organelles. In our previous studies, we have confirmed that CCAAT/enhancer binding protein α (C/EBP-α) can inhibit the activation of HSCs, but whether it can affect the activation of HSCs by regulating mitochondrial dynamics is still unclear.

Association between ultra-processed foods and female infertility: a large cross-sectional study.

Background: Infertility is a significant challenge in women's reproductive health, but the relationship between ultra-processed food (UPF) intake and female infertility remains unclear. This study aims to investigate the association between UPF intake and female infertility.

Methods: Using data from 3601 women in the NHANES database, UPF intake was defined as the percentage of energy consumed by UPF in each participant's daily total food intake energy.

Chaperone-Mediated Autophagy: A Critical Regulator of Neuroinflammation and Neurodegeneration.

Neurodegenerative diseases, including Alzheimer's disease (AD) and Parkinson's disease (PD), are characterized by hallmark pathological features such as the accumulation of misfolded proteins and neuroinflammation. Chaperone-mediated autophagy (CMA), a selective lysosomal pathway, facilitates the degradation of proteins containing KFERQ-like motifs via the receptor lysosome-associated membrane protein type 2A (LAMP2A). In the recent review, the pivotal role of CMA in regulating proteostasis and modulating inflammatory responses is highlighted.

Economic burden of pertussis and its determinants in Zhejiang Province, China: A cross-sectional study.

The purpose of this research is to analyze the economic burden and associated factors of pertussis in specific areas within Zhejiang Province. Additionally, we aim to identify the key factors that impact total expenses and provide evidence to guide vaccination strategies, thereby curbing the escalating prevalence of pertussis. This study conducted a survey and hospital medical records review to investigate the direct economic burden(healthcare costs) and indirect economic burden (non-healthcare costs) on patients' families, as well as a summary utility index value.

Developmental pathways in plants: Lessons from Arabidopsis for crop innovation.

The emergence of molecular biology, along with the use of Arabidopsis thaliana as a model organism, has significantly enhanced our understanding of plant development. Research on Arabidopsis has led to the identification of key regulatory genes involved in various developmental processes. In the past decade, advances in genome sequencing and the decoding of numerous plant genomes have enabled the application of these findings from Arabidopsis to crop species.

Threshold dynamics of a Wolbachia-driven mosquito suppression model on two patches.

The release of Wolbachia-infected mosquitoes is a promising and biologically safe measure for controlling wild mosquitoes. Numerous studies have been devoted to finding optimal control strategies using mathematical tools. However, the effects of dispersal of uninfected and infected mosquitoes remain poorly understood.

Integrating GWAS and Transcriptomic Data Using PrediXcan and Multimodal Deep Learning Reveals Genetic Basis and Drug Repositioning Opportunities for Alzheimer's Disease.

Alzheimer's disease (AD), the leading cause of dementia, imposes a significant societal and economic burden; however, its complex molecular mechanisms remain unclear. This study integrates multi-omics data with advanced artificial intelligence (AI) methods to uncover the molecular basis underlying AD phenotype regulation and explore personalized drug repositioning strategies based on individual genetic backgrounds. First, we applied the PrediXcan method to identify candidate genes closely associated with AD cognitive diagnosis, selecting from 61 brain-related traits.

NR4A1 Alleviates Subretinal Fibrosis by Inhibiting Macrophage to Myofibroblast Transition.

Purpose: Subretinal fibrosis (SF) secondary to neovascular age-related macular degeneration (nAMD) is the most predominant cause of central visual impairment in all patients with AMD. NR4A1 is a member of the nuclear orphan receptor superfamily, and has shown inhibitory effects on fibrosis in tissues such as the dermis, intestines, and heart. Cytosporone B (Csn-B) is a natural agonist of NR4A1.

Differentiation of hESC-Derived Neural Crest Cells into Trabecular Meshwork Cells.

Primary open-angle glaucoma is a prevalent type of degenerative eye disease that results in lifelong blindness, and its critical pathogenic cause is trabecular meshwork (TM) dysfunction or decreased TM cellularity. Considering that TM develops from neural crest cells (NCCs), we investigate the potential of human embryonic stem cell (hESC)-derived NCCs transplantation for TM regeneration. We used a chemically defined method to induce the differentiation of NCCs and injected 1.

A novel small cysteine-rich protein, PbEL04, identified from Plasmodiophora brassicae, associates with AtSAMS2 to regulate Arabidopsis clubroot resistance.

Plasmodiophora brassicae is a devastating intracellular pathogen that causes clubroot disease in Brassicaceae plants, leading to significant economic losses in agriculture. Enhancing plant resistance against P. brassicae has become a crucial global challenge.

Triple therapy disrupts gut microbiota more severely than quadruple therapy in children with Helicobacter pylori infection.

Aims: Helicobacter pylori (Hp) infection is associated with gastrointestinal and systemic disorders in children. This study compared the effects of triple versus quadruple antibiotic therapies on gut microbiota in children with Hp infection.

Methods And Results: Thirty pediatric patients with Hp infection were recruited and randomized into triple or quadruple therapy groups for eradication treatment.

RpS24 Is Required for Meiotic Divisions and Spermatid Differentiation During Drosophila Spermatogenesis.

In Drosophila, testes contain highly heterogeneous ribosome populations. Several ribosomal proteins (RPs) have been shown to play specific and distinct roles during different stages of spermatogenesis. However, the detailed functions and mechanisms of RPs in spermatogenesis remain unclear.

Neural network disruptions between default mode network and salience network in mild cognitive impairment with neuropsychiatric symptoms.

Background: Neuropsychiatric symptoms (NPS) in mild cognitive impairment (MCI) are associated with accelerated Alzheimer's disease (AD) progression. Identifying multimodal brain imaging patterns associated with NPS in MCI may help understand pathophysiology correlates AD.

Methods: This cross-sectional resting-state functional magnetic resonance imaging study included 376 participants: 130 MCI with neuropsychiatric symptoms (MCI+NPS), 154 MCI without neuropsychiatric symptoms (MCI-NPS), 92 cognitively normal (CN).

ZC3H12D gene expression exhibits dual effects on the development and progression of lung adenocarcinoma.

While precision oncology requires robust biomarkers, current predictors for lung adenocarcinoma (LUAD) often show limited clinical utility. This study investigates the multifaceted roles of ZC3H12D, a novel immunomodulatory molecule, in LUAD progression and tumor microenvironment regulation. Multi-omics analyses integrated ZC3H12D transcriptomic (511 tumors vs 59 normals), proteomic (74 tumors vs 69 normals), and single-cell RNA-seq data (15 tumors vs 11 normals).

NRF1 coordinates mitochondrial adaptations to dampen intracellular ROS and inflammatory responses during ischemia reperfusion.

Ischemia reperfusion injury (IRI) is commonly seen in surgical procedures involving cardiopulmonary bypass and post-shock reperfusion. Sudden restoration of blood flow after a period of ischemia triggers a rapid accumulation of reactive oxygen species (ROS) and oxidative stress that promote pathological injury. Macrophage-derived inflammatory responses are also thought to contribute to such injury, but how ROS influences tissue macrophages and their elaboration of inflammatory cytokines in IRI remains poorly understood.

GMAMDA: Predicting Metabolite-Disease Associations Based on Adaptive Hardness Negative Sampling and Adaptive Graph Multiple Convolution.

Metabolites are small molecules produced during organism metabolism, with their abnormal concentrations closely linked to the onset and progression of various diseases. Accurate prediction of metabolite-disease associations is crucial for early diagnosis, mechanistic exploration, and treatment optimization. However, existing algorithms often overlook the integration of node features and neglect the impact of different hop domains on nodes in the processing of heterogeneous graphs.

LEC2 induces somatic cell reprogramming through epigenetic activation of plant cell totipotency regulators.

Many plant species can develop embryos from somatic cells without fertilization. During this process, known as somatic embryogenesis, changes in the DNA methylation patterns are characteristic of reprogramming somatic cells into an embryogenic state. However, the underlying mechanisms connecting DNA methylation and activating totipotency-regulating genes have remained largely unknown.

Harnessing Xylanase Potential in : Insights from Computational and Functional Analysis.

Xylanases are crucial for the breakdown of hemicellulose, enabling the conversion of lignocellulosic biomass into fermentable sugars for biofuels and other industrial applications. For the first time, we investigated the biochemical and genetic characteristics of 22 xylanase genes from within glycoside hydrolase (GH) families GH10, GH11, and GH43. Xylanase genes structural diversity clustered the phylogenetic tree into GH10, GH11, GH43-I, and GH43-II groups.