Nitrogen-Fixing Bacterium GXGL-4A Promotes the Growth of Cucumber Plant Under Nitrogen Stress by Altering the Rhizosphere Microbial Structure.

The rhizosphere microbiome plays an important role in carbon- and nitrogen-cycling in soil and in the stress response of plants. It also affects the function of the ammonium transporter (AmtB) that senses nitrogen levels inside and outside the cells of the associative nitrogen-fixing bacterium GXGL-4A. However, the potential mechanism of the interaction between the AmtB deletion mutant of GXGL-4A (∆B) and microorganisms in the rhizosphere of plants under low-nitrogen stress is still unclear.

Photodynamic-Photothermal Switching in Osmium-Ferritin Nanoplatform Activates Near Infrared-Driven Tumor Immunity Reprogramming.

Conventional metallo-photosensitizers (PSs) face short-wavelength excitation (<650 nm), poor tumor-targeting specificity, and monotherapy limitations; engineering hypoxia-tolerant multimodal systems integrated with tumor-targeting precision and microenvironment-adaptive metallo-PSs remains challenging for clinical transformation. In this study, we developed an Os(II)-cyanine complex , which exhibits aggregation-dispersion-induced photodynamic-photothermal conversion properties. To enhance its tumor-targeting capabilities, we encapsulated in human heavy-chain ferritin (HFn) to form stable nanoparticles (NPs).

Chaperone-Mediated Autophagy: A Critical Regulator of Neuroinflammation and Neurodegeneration.

Neurodegenerative diseases, including Alzheimer's disease (AD) and Parkinson's disease (PD), are characterized by hallmark pathological features such as the accumulation of misfolded proteins and neuroinflammation. Chaperone-mediated autophagy (CMA), a selective lysosomal pathway, facilitates the degradation of proteins containing KFERQ-like motifs via the receptor lysosome-associated membrane protein type 2A (LAMP2A). In the recent review, the pivotal role of CMA in regulating proteostasis and modulating inflammatory responses is highlighted.

Gastroenterologists' perspectives of high-dose dual therapy for Helicobacter pylori infection in China: Results of a China survey.

This study investigates the knowledge of the high-dose dual therapy (HDDT) in the eradication of Helicobacter pylori (H pylori) infection among gastroenterologists in China. A survey was conducted among gastroenterologists in China using the "Questionnaire Star," and the questionnaire link was sent by WeChat. Descriptive methods were used for statistical analysis.

High-dose dual therapy for Helicobacter pylori eradication inducing less impact on the gut microbiota.

Background: Helicobacter pylori (H. pylori) eradication regimens may have different effects on the gut microbiota. Few studies have analyzed the safety of high-dose dual therapy (HDDT) from a micro-ecological perspective.

Videos in Short-Video Sharing Platforms as Sources of Information on Colorectal Polyps: Cross-Sectional Content Analysis Study.

Background: Short videos have demonstrated huge potential in disseminating health information in recent years. However, to our knowledge, no study has examined information about colorectal polyps on short-video sharing platforms.

Objective: This study aimed to analyze the content and quality of colorectal polyps-related videos on short-video sharing platforms.

Itga5-PTEN signaling regulates striatal synaptic strength and motor coordination in Parkinson's disease.

: Parkinson's disease (PD) is marked by the loss of dopaminergic neurons in the substantia nigra pars compacta, leading to motor and cognitive dysfunctions. The molecular mechanisms underlying synaptic alterations in PD remain elusive, with a focus on the role of Itga5 in synaptic integrity and motor coordination and TAT-Itga5 was designed to suppress PTEN activity in this investigation. : This study utilized MPTP-induced PD animal models to investigate the expression and role of Itga5 in the striatum.

Application of furazolidone in Helicobacter pylori infection eradication.

Increasing antibiotic resistance is the primary reason for treatment failure of Helicobacter pylori (H. pylori) infection. To enhance the eradication rate, minimize the development of secondary resistance, and alleviate the socioeconomic burden, it is crucial to select H.

Global research trends and hotspots of Helicobacter pylori eradication based on clinical trial registration platforms: A cross-sectional analysis.

Background: This study aimed to obtain an overview of clinical trials on Helicobacter pylori (H. pylori) eradication and analyze the global trends and hotspots in this field.

Methods: We collected the data from clinical trials focused on H.

The Dual Role of Non-coding RNAs in the Development of Periodontitis.

This review aims to sum up how Non-coding RNAs (ncRNAs) regulate the development of periodontitis and provides a new perspective for understanding the pathogenesis of periodontitis. We explored the ncRNA's dual role in the development of periodontitis by summarizing evidence from previous and studies as well as clinical samples. In our review, the downregulation of 18 miRNAs, 22 lncRNAs and 10 circRNAs demonstrates protective roles in periodontitis.

Molecular detection and genetic characteristics of a novel porcine circovirus (porcine circovirus 4) and porcine reproductive and respiratory syndrome virus in Shaanxi and Henan Provinces of China.

Porcine circovirus 4 (PCV4) is a recently discovered circovirus that was first reported in 2019 in several pigs with severe clinical disease in Hunan province of China, and also identified in pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV). To further investigate the epidemic profile and genetic characteristics of the two viruses, 150 clinical samples were collected from 9 swine farms in Shaanxi and Henan provinces of China, and a SYBR Green I-based duplex quantitative real-time polymerase chain reaction (qPCR) was developed for detecting PCV4 and PRRSV simultaneously. The results showed the limits of detection were 41.

Impact of body size on efficacy of high-dose dual therapy for Helicobacter pylori eradication.

Background: High-dose dual therapy (HDDT) is an emerging and promising therapeutic regime for Helicobacter pylori (H. pylori) eradication. However, the pharmacokinetics of the components of HDDT, amoxicillin and proton pump inhibitor, are likely to be affected by body size.

Construction, Characterization, and Application of an Ammonium Transporter (AmtB) Deletion Mutant of the Nitrogen-Fixing Bacterium Kosakonia radicincitans GXGL-4A in Cucumis sativus L. Seedlings.

Nitrogen is an important factor affecting crop yield, but excessive use of chemical nitrogen fertilizer has caused decline in nitrogen utilization and soil and water pollution. Reducing the utilization of chemical nitrogen fertilizers by biological nitrogen fixation (BNF) is feasible for green production of crops. However, there are few reports on how to have more ammonium produced by nitrogen-fixing bacteria (NFB) flow outside the cell.

Prognostic value and immune infiltration of the gasdermin family in lung adenocarcinoma.

Background: The GSDM family includes six members, GSDMA, GSDMB, GSDMC, GSDMD, GSDME (DFNA5), and PJVK (Pejvakin, DFNB59), which can induce pyroptosis, thereby regulating the tumorigenesis of various cancers. However, the clinical characteristics and role of the GSDM family in LUAD are not well understood.

Methods: In this study, several important bioinformatics databases were used to integrate the analysis of the expression, prognostic value, and immune infiltration of GSDMs in LUAD.

Evaluation of the potential of ultrasound-mediated drug delivery for the treatment of ovarian cancer through preclinical studies.

Ovarian cancer (OC) has the greatest mortality rate among gynecological cancers, with a five-year survival rate of <50%. Contemporary adjuvant chemotherapy mostly fails in the case of OCs that are refractory, metastatic, recurrent, and drug-resistant. Emerging ultrasound (US)-mediated technologies show remarkable promise in overcoming these challenges.

Urgent Endoscopy in Nonvariceal Upper Gastrointestinal Hemorrhage: A Retrospective Analysis.

Objective: The role of urgent endoscopy in nonvariceal upper gastrointestinal hemorrhage (NVUGIH) remains controversial. We designed a retrospective study to compare the outcomes between urgent endoscopy (within 12 h) and non-urgent endoscopy for patients with NVUGIH.

Methods: A total of 540 hospitalized patients with NVUGIH were included in our study.

Does off-hours endoscopic hemostasis affect outcomes of nonvariceal upper gastrointestinal bleeding?

Different researches showed controversial results about the 'off-hours effect' in nonvariceal upper gastrointestinal bleeding (NVUGIB). A total of 301 patients with NVUGIB were divided into regular-hours group and off-hours group based on when they received endoscopic hemostasis, and the relationship of the clinical outcomes with off-hours endoscopic hemostasis was evaluated. Patients who received off-hours endoscopy were sicker and more likely to experience worse clinical outcomes.

Bromodomain-containing protein 9 promotes hepatocellular carcinoma progression via activating the Wnt/β-catenin signaling pathway.

Background: Epigenetic dysregulation participates in the initiation and progression of hepatocellular carcinoma (HCC). Bromodomain-containing protein 9 (BRD9) can identify acetylated lysine residues, contributing to several cancers. The function and molecular mechanism of BRD9 in HCC remain poorly understood.

Rutin pretreatment promotes microglial M1 to M2 phenotype polarization.

Microglial cells are important resident innate immune components in the central nervous system that are often activated during neuroinflammation. Activated microglia can display one of two phenotypes, M1 or M2, which each play distinct roles in neuroinflammation. Rutin, a dietary flavonoid, exhibits protective effects against neuroinflammation.

Association of HMOX-1 with sporadic Alzheimer's disease in southern Han Chinese.

Background And Purpose: The aim of this study was to discover the associations between HMOX-1 and Alzheimer's disease (AD).

Methods: A total of 500 AD patients and 500 healthy controls were recruited in this study. Polymer chain reaction was used.

The Joubert Syndrome Gene arl13b is Critical for Early Cerebellar Development in Zebrafish.

Joubert syndrome is characterized by unique malformation of the cerebellar vermis. More than thirty Joubert syndrome genes have been identified, including ARL13B. However, its role in cerebellar development remains unexplored.

Triterpene saponins from tea seed pomace ( Abel) and their cytotoxic activity on MCF-7 cells in vitro.

Triterpenoid saponins are the main active ingredients extracted from Abel. In this study, crude saponins (Tc) was extracted from tea seed pomace and purified to obtain total saponins (T0). We used a COSMOSIL C18-OPN to separate T0 into three fractions-highly polar saponins (T1), moderately polar saponins (T2), and weakly polar saponins (T3).

[Vegetation damage, post-disaster change degree and driving factors in the cold temperate wind disaster area of Changbai Mountain, China.].

The typhoon outbreaks of 1986 caused many gaps in the cold temperate coniferous forest and Betula errmanii forest on the western and southern slopes in the Changbai Mountain. In 2017, a research area at the 1600-1800 m altitude in Changbai Mountain was established to explore the damage and changes of forest vegetation disturbed by the typhoon in cold temperate zone and to reveal the regulation of damage on vegetation and post-disaster change as well as the driving factors. The remote sensing data were used to classify research area based on the degree of vegetation damage and post-disaster change.