Carbon and nitrogen stable isotopes in each organ of with different ages.

Understanding carbon and nitrogen stable isotopic abundance (δC and δN) and carbon-to-nitrogen ratio (C/N) in different organs of across ages and elucidating the patterns along the age sequence can provide scientific insights into the ecophysiological mechanisms of its degradation and sustainable utilization. With four plantations with different ages (11 a, 28 a, 38 a and 57 a) in a desert steppe located at the southern edge of Mu Us Desert in Ningxia, we investigated the variation in δC, δN and C/N of different organs (leaf, branch, pod, and seed) and the driving factors. Results showed that plant δC, δN, and C/N of ranged from -27.

Release kinetics and bioaccessibility of heavy metal from antifouling paint particles in simulated digestive fluids: An emerging threat to marine biota.

Antifouling paints can effectively prevent marine organisms from biofouling. However, the release of antifouling paint particles (APPs) and their toxic additives has emerged as a significant source of marine contamination, posing potential threats to marine biota. Hence, this study investigated the release kinetics and bioaccessibility of heavy metals from APPs in simulated digestive fluids, mimicking the gastrointestinal conditions of marine polychaete, fish, and seabird.

extract improves insulin tolerance and pancreatic islet function in ageing and HFD-induced diabetic mice.

Siamenoside I (SI) is the sweetest compound isolated from fruit. The extremely low natural abundance limits its application. We have previously produced an M3 extract (containing 68.

Identification of risk factors and incidence of non-tunnelled central venous catheter occlusion in pediatric intensive care unit: A retrospective cohort study.

Background: Non-tunnelled central venous catheter (CVC) occlusion represents a significant clinical challenge in pediatric intensive care units (PICUs), emphasizing the need for improved understanding of its incidence and risk factors.

Purpose: This study aimed to investigate the incidence and risk factors for non-tunnelled CVC occlusion among critically ill children.

Design And Methods: This retrospective cohort study was conducted in a single PICU in China.

Lenvatinib promotes hepatocellular carcinoma pyroptosis by regulating GSDME palmitoylation.

Lenvatinib, as a multi-kinase inhibitor, has been approved as a first-line drug for patients with advanced hepatocellular carcinoma (HCC). Gasdermin E (GSDME)-mediated pyroptosis, a form of programmed cell death, can be induced by chemotherapy drugs or certain kinase inhibitors. However, the role of Lenvatinib in inducing pyroptosis in HCC warrants further investigation.

DSS1 is required for proper Integrator-PP2A function.

Integrator-PP2A (INTAC) is a highly modular complex orchestrating the transition of paused RNA polymerase II into productive elongation or promoter-proximal premature termination, with its loss resulting in transcription dysregulation and genome instability. Here, we identify human DSS1-a flexible 70-residue protein found in multiple functionally diverse complexes including the 26S proteasome-as an integral subunit of the INTAC backbone. Structural analysis of DSS1-INTAC, both alone and in association with paused polymerase, demonstrates intimate interactions between DSS1 and the INTAC backbone.

[Investigation of Infection in HBV-Reactive Blood Donors in Wuhan].

Objective: To investigate the pattern of hepatitis B virus (HBV) infection and the prevalence of hepatitis D virus (HDV) infection among voluntary blood donors who tested reactive for HBV in Wuhan, and to provide data support for the prevention and treatment of HBV and HDV infections.

Methods: Electrochemiluminescence (ECL) method was used to detect hepatitis B serological markers in the samples with HBsAg and/or HBV DNA reactivity, and the HBV infection in different groups was statistically analyzed. The HDV IgM and IgG antibodies were screened by ELISA, and the prevalence of HDV infection in the retained samples was analyzed.

Navigating the complexity of exposure to multiclass organic pollutants in respirable size-resolved particles and implications for oxidative potential.

Chronic exposure to inhalable atmospheric particulate matter is linked to millions of annual premature deaths globally. Yet the sources and factors driving oxidative potential (OP) remain poorly understood, especially regarding the coexistence of multi-organic pollutants. This longitudinal study examined the size-distribution, respiratory deposition efficiency and daily exposure of 41 chemicals covering polycyclic aromatic hydrocarbons (PAHs), brominated flame retardants (BFRs), and phthalates (PAEs), as well as the dithiothreitol-based OP in size-fractioned PM from distinct waste recycling plants in Guangzhou, South China.

Pulmonary and Extrapulmonary Effects of Prolonged Prone Positioning in a Porcine Model of Acute Respiratory Distress Syndrome.

Background: Prone positioning (PP) improves survival in severe acute respiratory distress syndrome (ARDS), but its prolonged effects on pulmonary and extrapulmonary organs remain unclear. This study aimed to investigate the pathophysiological effects of 24-hour PP in a porcine ARDS model.

Methods: Ten female Bama mini swine (49.

Development and verification of a prediction model for delirium in critically ill children.

Purpose: Delirium is a common syndrome in the intensive care unit (ICU), with a high incidence in critically ill children. This study aims to develop and validate a prediction model for delirium in critically ill children, which could potentially enhance early identification and management strategies.

Methods: In this prospective cohort study, we collected 1,047 critically ill children admitted to the pediatric intensive care unit (PICU) of a tertiary children's hospital from November 2021 to November 2023.

Complete xylan utilization pathway and regulation mechanisms involved in marine algae degradation by cosmopolitan marine and human gut microbiota.

β-1,3-xylan, typically found in marine algae as a major cell wall polysaccharide, represents an overlooked pool of organic carbon in global oceans. Whilst our understanding of microbial catabolism of xylans has improved significantly, particularly from biotransformations of terrestrial plant biomass that are typically composed of β-1,4-xylans, knowledge on how microbes utilize β-1,3-xylan remains limited. Here, we describe the discovery of a complete pathway for β-1,3-xylan catabolism and its regulation in a marine bacterium, Vibrio sp.

Impact of Tagatose on Physicochemical, Nutritional, and In Vitro Digestive Properties of Toast Bread.

It is more attractive to develop effective strategies to reduce sugar intake without compromising food quality with the rising prevalence of obesity and diabetes around the world. Due to its high cost, tagatose has not been widely adopted as a sucrose substitute in toast bread. In the present research, five types of toast containing different proportions (0%, 4%, 8%, 12%, and 16%) of tagatose with sweetness similar to that of sucrose were prepared.

[Study on Reentry Strategy and Results of Blood Donors with Single Reagent Reactivity in Wuhan Area].

Objective: To study the results, re-donation situation and characteristics of single-reagent reactive blood donors who were put into the reentry strategy in Wuhan area, explore the rationality and effectiveness of the current reentry strategy, and provide data support for the improvement of the reentry process of blood donors.

Methods: From January 2020 to December 2023, blood donors who conform the reentry criteria and voluntarily applied for returning to Wuhan Blood Center were tested and the results were analyzed. According to the reentry strategy, serological testing and nucleic acid testing were carried out in parallel, serological testing was performed by ELISA with reagents from two different manufacturers, and the primary reactive samples were tested by double-well retest, and HBV/HCV/HIV nucleic acid detection was performed by RT-PCR with an individual donor test mode.

Exposure to plasticizers in city waste recycling: Focused on the size-fractioned particulate-bound phthalates and bisphenols.

Phthalate (PAEs) and Bisphenols (BPs) are plasticizers or additives in consumer products. They are typical endocrine disruptors, and potential health hazards may occur when people are exposed to them through inhalation, ingestion, and dermal contact. The current research on inhalation exposure pays limited attention to the particle distribution of PAEs and BPs in air, although particulate-bound pollutants are usually size-dependent.

Identification of indoles as potential endogenous ligands of ERRγ and their modulation on drug binding.

Estrogen-related receptor γ (ERRγ) is an orphan nuclear receptor in the ERR subfamily that plays a crucial role in regulating energy metabolism. To date, no endogenous ligand has been identified for ERRγ, posing a challenge for developing targeted therapeutics. Here, we identified that indole and skatole produced by the gut microbiota are potential endogenous ligands of ERRγ using biochemical, cellular, structural, and computational approaches.

NMDAR2B/PKA/CREB signaling pathway contributes to esophageal neuropathic pain in gastroesophageal reflux disease.

Background: Esophageal hypersensitivity is an important cause of refractory gastroesophageal reflux disease, in which patients do not respond to standard acid-suppressive therapy and suffer from continuous noncardiac chest pain and regurgitation. The N-methyl-D-aspartate receptor (NMDAR) may play a crucial role in the development of visceral hypersensitivity in functional gastrointestinal disorders. However, the specific mechanisms of visceral hypersensitivity in upper digestive tract diseases remain poorly understood.

The clinical features and outcomes of diabetes patients infected with COVID-19: a systematic review and meta-analysis comprising 192,693 patients.

Objectives: We sought to explore the relevance of analyses that include critical laboratory parameters and drug treatment, clinical characteristics of diabetic patients who are infected with COVID-19, to the development of individualized treatment strategies for diabetic patients infected with COVID-19.

Methods: We searched Cochrane, Embase, FMRS, Pubmed, Springer, Web of Science databases for systematic reviews and meta-analyses to estimate the clinical characteristics and prognosis of confirmed covid-19 infections in patients with and without diabetes.

Results: Our meta-analysis included a total of 32 studies with 192,693 COVID-19 patients.

Variation in leaf and root functional traits of across different provenances in a common garden.

Environmental and genetic differentiation jointly influence intra-specific variations of plant functional traits. Research on this topic is of great importance for the assessment plant adaptation to climate change and for developing long-term conservation strategies. In a common garden experiment, we investigated the variations in root and leaf functional traits of across 14 provenances, as well as their relationships with the climatic and geographic factors of seed origin.

High-dose dual therapy for Helicobacter pylori eradication inducing less impact on the gut microbiota.

Background: Helicobacter pylori (H. pylori) eradication regimens may have different effects on the gut microbiota. Few studies have analyzed the safety of high-dose dual therapy (HDDT) from a micro-ecological perspective.

Optimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells.

To meet the requirements of the biopharmaceutical industry, improving the yield of recombination therapeutic protein (RTP) from Chinese hamster ovary (CHO) cells is necessary. The human cytomegalovirus (CMV) promoter is widely used for RTP expression in CHO cells. To further improve RTP production, we truncated the human CMV intron and further evaluated the effect of four synthetic introns, including ctEF-1α first, EF-1α first, chimeric, and β-globin introns combined with the CMV promoter on recombinant expression levels in transient and stably recombinant CHO cells.

Comammox Nitrospira are the dominant ammonia oxidizers in the Yangtze estuarine biofilms.

Biofilms are indispensable ecological habitats for microbes that have garnered global attention and play a potential role in influencing the biogeochemical cycling of nitrogen. However, the biogeochemical significance of biofilms and the mechanisms by which they regulate nitrogen cycling remain elusive. In this study, we utilized DNA-stable isotope probing (DNA-SIP) labelling techniques in conjunction with metagenomics to reveal a nitrifying ecological niche in biofilms taken from the Yangtze Estuary, with those from sediment and water samples for comparison.

[Characteristics, Transport Routes, and Potential Sources of PM and O Pollution in Suzhou].

Based on the air quality and meteorological data in Suzhou from 2015 to 2022, the long-term variations in PM and O, meteorological characteristics, and their correlations were analyzed in this study. The HYSPLIT model was used to explore the main transport pathways and potential source areas of PM and O. The results showed that： ① The annual averaged concentrations of PM in Suzhou decreased steadily during the study period, and the annual average concentration from 2020 to 2022 reached the national second-level standard limit.

[Application of interrupter resistance pulmonary function testing in the diagnosis of asthma in preschool children: a single-center study].

Objectives: To investigate the value of interrupter resistance (Rint) pulmonary function testing in the diagnosis of asthma in preschool children, and to compare the significance of Rint pulmonary function testing versus impulse oscillometry (IOS) in the diagnosis of asthma.

Methods: A prospective study was conducted among 108 children with recurrent wheezing, aged 4 to <6 years, who were admitted from July 2022 to November 2023. According to the treatment outcome, they were divided into an asthmatic group (61 children) and a non-asthmatic group (47 children).

Measurements of enteral feeding intolerance in critically ill children: a scoping review.

Objective: To examine the measurements on enteral feeding intolerance (EFI) in critically ill children.

Methods: The Joanna Briggs Institute methods for conducting a scoping review were followed. Articles published since 2004 which assessed EFI in critically ill children were identified.