Integrated screening of quality markers from Paris polyphylla var. yunnanensis using UHPLC-Q/TOF-HRMS, spectrum-effect relationship analysis, network pharmacology, and quantitative analysis.

This study aims to comprehensively screen quality markers using an integrated multi-strategy approach combining plant metabolomics, spectrum-effect relationship analysis, network pharmacology, and quantitative analysis, thereby providing a basis for quality control of Paris polyphylla var. yunnanensis and its closely related species. Firstly, 14 differential metabolites were screened from the roots, stems, and leaves of Paris polyphylla var.

Metabolic rewiring and morphological adaptations drive bacterial strain-specific cadmium defense in the Yangtze River estuary.

Cadmium (Cd) contamination in coastal regions poses severe environmental risks, yet bacterial defense mechanisms against Cd remain poorly understood. This study unveils distinct tolerant strategies of two highly Cd-tolerant bacteria isolated from the Yangtze River estuary: Comamonas sp. Y49 and Aeromonas sp.

The pH-responsive regulator PlPacC and GATA transcription factor PlAreB are involved in the regulation of the biosynthesis of the antifungal lipopeptaibols leucinostatins in Purpureocillium lilacinum.

The biocontrol fungus Purpureocillium lilacinum PLBJ-1 produces leucinostatins, a class of non-ribosomal peptides (NRPs) with broad-spectrum antimicrobial activities. However, the molecular mechanisms underlying the optimization of culture conditions for leucinostatin production remain unexplored. Previous research showed that PLBJ-1 synthesizes leucinostatins more effectively in hand-made Potato Dextrose Broth (PDB-M) than in commercially available PDB (PDB-C).

The cytoplasmic N- and C-termini are dispensable for SLAH3 to mediate nitrate-dependent ammonium detoxification in Arabidopsis.

Ammonium (NH) toxicity significantly limits nitrogen use efficiency (NUE) in agriculture. Nitrate (NO) supplementation mitigates this toxicity, with the anion channel SLAH3 playing a central role by mediating NO efflux to counteract NH-induced rhizosphere acidification. SLAH3, a plasma membrane protein with ten transmembrane domains and cytosolic N- and C-termini, is intrinsically silent.

Repurposing disulfiram: An innovative inhibitory approach against a broad spectrum of viruses.

Disulfiram (DSF), an FDA-approved therapeutic agent for alcohol dependence, has recently attracted considerable interest due to its broad-spectrum inhibitory effects against various viruses. Increasing evidence suggests that DSF can inhibit viral replication through two major mechanisms: the inhibition of viral protein catalytic activity and the ejection of Zn from viral proteins. This review comprehensively summarized the molecular mechanisms underlying DSF's antiviral activity against viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), hepatitis C virus (HCV), influenza virus, human immunodeficiency virus (HIV), and Kaposi sarcoma-associated herpes virus (KSHV), with a particular focus on its dual targeting of Cys residues and Zn coordination sites.

UCP2 is identified as a therapeutic target for abdominal aortic aneurysm by comprehensive bioinformatic analysis and experimental validation.

Abdominal aortic aneurysm (AAA) is a potentially life-threatening vascular condition that currently lacks effective pharmacological treatment. The disease is strongly associated with chronic inflammation, where immune cells like macrophages play a crucial role. Efferocytosis, the process by which apoptotic cells are cleared, is involved in regulating inflammation.

Mapping between chain configuration and mechanical properties of poly(L-lactic acid) monofilaments via hydrolysis induction.

Poly(L-lactic acid) (PLLA), as a substrate material, has been widely utilized in the field of biodegradable vascular stents. Prior to implantation, it is particularly crucial for these devices to assess the relationship of the mechanical properties and microstructures during full degradation cycle. Although previous studies have primarily focused on structural parameters such as crystallinity and molecular weight, there are relatively few reports that explore the impact of microstructure on mechanical performance from the perspective of chain configuration during the degradation.

Multi-scale design of the structure and mechanical performance of the deep-sea hydrothermal mussel (Bathymodiolus aduloides) shell.

Deep-sea hydrothermal vents are renowned for being among the most extreme environments on Earth. However, the mussel shells found in these vent sites demonstrate remarkable productivity, despite being subjected to high pressure as well as unusual levels of heavy metals, pH, temperature, CO, and sulphides. To comprehend how these mussels endure such extreme conditions, a systematic comparative study was conducted, focusing on the unique chemical composition, structural designs, and mechanical properties of hydrothermal vent mussels (Bathymodiolus aduloides) in comparison to shallow-water mussels (Mytilus edulis).

The survival analysis of stage III and IV inoperable lung large cell neuroendocrine carcinoma and the role of LIPI in immunological stratification.

Background: Large cell neuroendocrine carcinoma (LCNEC) represents a rare and unique type of lung tumor with an unfavorable prognosis. It is essential to summarize the treatment modalities and prognosis for inoperable stage III and IV LCNEC, explore the role of frontline immunotherapy, and examine the stratification role of the Lung Immune Prognostic Index (LIPI) and its relationship with the tumor microenvironment (TME).

Methods: This study retrospectively analyzed 160 patients with inoperable lung LCNEC (L-LCNEC) admitted to three hospitals from December 2012 to November 2023.

Mesoporous Zr MOFs regulated by linear ligand replacement strategy to achieve single Ru(bpy) molecule encapsulation for ratio ECL detection of miRNA-21.

Rational optimization of the pore size and topology of porous nanocarriers is crucial for improving the loading amount of luminophore and enhancing electrochemiluminescence (ECL) performance. In this study, an equimolar linear ligand replacement strategy was employed to synthesize novel mesoporous metal-organic frameworks (MOFs) for encapsulating Ru(bpy) (Ru@Zr MOFs) under room temperature without an acid modulator. Ingenious ligand substitution allows precise control of pore size, enabling encapsulation at the single-molecule level within mesoporous cages.

Blueberry anthocyanins-functionalized hydrogel labels for smartphone-assisted real-time visual freshness monitoring of perishable proteins.

Given rising consumer demands for meat safety and quality assurance, developing an intuitive, cost-effective, and user-friendly sensor platform for real-time monitoring of perishable meat freshness is important. Herein, this study developed an innovative chitosan/agarose/blueberry anthocyanin (CS/AG/BA) hydrogel label system for visual real-time freshness tracking of perishable proteins through smartphone-assisted colorimetric analysis. Through systematic optimization of CS/AG compositional ratios (3:7-7:3) and pH conditions (2.

Multi-component strategy for the preparation of covalent organic frameworks-based stationary phase for high resolution separation of isomers.

Functionalization of covalent organic frameworks (COFs) is a critical means to prepare COF-based gas chromatography stationary phases for high-resolution separation. Here, we report a simple and controllable multi-component strategy for the functionalization of COF stationary phases. As a proof of concept, a COF fabricated from 1,3,5-tris(4-aminophenyl) benzene (Tab) and 2,5-dihydroxy-1,4-benzenedicarboxaldehyde (DHTA), Tab-DHTA, was chosen as a model.

Light-emitting diode-derived blue light overexposure accelerates corneal endothelial cell aging by inducing abnormal ROS accumulation.

Blue light, defined as short-wavelength visible light ranging from 400 to 500 nm, is recognized for its high energy within the visible light spectrum. The prevalent use of light-emitting diodes (LEDs) has significantly increased exposure to blue light. Corneal endothelial cells (CECs) playing a crucial role in maintaining corneal transparency to get clear visual field.

Identification of RAV transcription factors (B3-domain-containing) and functional analysis of OsRAV2 in rice blast and drought stress.

RAV transcription factors play roles in a variety of diverse biological processes. However, their role in rice's response to drought and blast stress remains largely unexplored. In this study, we performed a genome-wide characterization and identification of rice RAV transcription factor family genes.

Adsorption behavior and neurotoxic synergy of thallium and polystyrene microplastics in Caenorhabditis elegans.

Microplastics (MPs) have emerged as ubiquitous environmental contaminants, while thallium (Tl), a highly toxic metalloid, is gaining attention as a novel pollutant due to its increasing release from electronic waste and mining activities. These pollutants frequently coexist in aquatic environments; however, their combined effects at environmentally relevant concentrations remain poorly understood. In this study, the adsorption behavior and joint neurotoxicity of polystyrene (PS) microplastics and Tl were systematically evaluated using Caenorhabditis elegans as a model organism.

Key factors affecting heavy metal contamination of mangrove sediments in the Zhangjiang Estuary: Implications for environmental management.

With the rapid economic development of coastal cities, the discharge of significant amounts of heavy metal pollutants has posed a severe threat to mangrove forests. However, the potential sources of these metals and the health risks they pose remain poorly understood. This study analyzed 14 heavy metals in mangrove and river sediments of Zhangjiang Estuary, southeastern China.

The value of spontaneous electromyographic activity in differentiating ischemic myelopathies from inflammatory demyelinating diseases: A retrospective study.

Background: Differentiating ischemic myelopathies from inflammatory demyelinating diseases is challenging due to overlapping imaging and clinical manifestations. Needle electromyography (EMG) is highly sensitive to spinal anterior horn damage.

Objectives: This study investigates the diagnostic value of spontaneous EMG activity in distinguishing ischemic myelopathies from inflammatory demyelinating diseases.

Mussel-inspired polydopamine-functionalized ultralight cellulose aerogel for simple and portable extraction of synthetic cathinones from biological samples.

Synthetic cathinones (SCs) are drugs of abuse that act on the central nervous system, producing psychoactive effects similar to those of amphetamines. Their greater accessibility compared with the traditional amphetamine-type stimulants has contributed to their increasing popularity in recent years. The analysis of SCs in biological samples is essential for documenting their consumption.

Integrated GC × GC-FID method for high-efficiency gasoline analysis: From standard quantification to non-target screening.

Conventional one-dimensional gas chromatography methods for gasoline quality monitoring require separate analyses for different component classes, limiting analytical efficiency and unconventional additive detection. This study presents a comprehensive two-dimensional gas chromatography with flame ionization detection (GC × GC-FID) platform enabling simultaneous quantification of regulated components and rapid screening of unconventional additives in a single analytical run. The method achieved excellent agreement with ASTM standards and high repeatability for BTEX (benzene, toluene, ethylbenzene, and xylenes) and oxygenates in gasoline.

A magnetic porous carbon material achieving rapid and convenient separation of volatile cinnamaldehyde in cinnamon.

Rapid and convenient enrichment and detection of volatile cinnamaldehyde (Cin) from a common herbal medicine, cinnamon, was achieved through a reliable MSPE-HPLC-DAD approach. The magnetic porous carbon material (Carbon-FeC/lignin) used for MSPE was prepared as follows. First, the metal organic framework (MIL-101-NH (Fe)) was synthesized using the solvothermal method.

Identifying meaningful drug response biomarkers from public pharmacogenomic datasets with biologically informed interpretable neural networks.

With the advancements of next-generation sequencing, publicly available pharmacogenomic datasets from cancer cell lines provide a handle for developing predictive models of drug responses and identifying associated biomarkers. However, many currently available predictive models are often just used as black boxes, lacking meaningful biological interpretations. In this study, we made use of open-source drug response data from cancer cell lines, in conjunction with KEGG pathway information, to develop sparse neural networks, K-net, enabling the prediction of drug response in EGFR signaling pathways and the identification of key biomarkers.

The relationship between fear of negative evaluation and social anxiety in parents of children with ASD: The chain mediating role of perceived social support and coping styles.

Parents of children with ASD face significantly greater parenting challenges than those raising typically developing children due to prolonged exposure to their children's developmental disorders, emotional distress, and atypical behaviors, underscoring the urgency of addressing their mental health concerns. This study examined the relationship between fear of negative evaluation (FNE) and social anxiety in parents of children with ASD, with a focus on the mediating roles of perceived social support and coping styles. A cross-sectional survey was conducted among 585 parents of children with ASD using validated instruments: the Brief Fear of Negative Evaluation Scale (BFNE), the Social Anxiety Scale, the Perceived Social Support Scale, and the Simple Coping Style Questionnaire.

MAP30 inhibits proliferation and metastasis of bladder cancer by increasing EGR1 expression and promoting the transcriptional activation of DUSP1.

The confirmed tumor-inhibitory effects of the 30 kDa Momordica anti-human immunodeficiency virus protein (MAP30) have yet to be complemented by an exploration into its mechanism of action on tumor development and metastasis. For this purpose, we delved into the intrinsic mechanism of MAP30 in bladder cancer (BC). Here, we demonstrated that MAP30 markedly suppressed the proliferation, migration, invasion, and angiogenic capabilities of human BC cells in vitro, and the tumor metastatic potential in vivo.