Association between remnant cholesterol and atherosclerosis plaques in single and multiple vascular territories.

Objectives: In recent years, the role of remnant cholesterol (RC) in the development and progression of cardiovascular diseases has gained increasing attention. However, evidence on the association between RC and subclinical atherosclerosis is limited. This study aims to examine the relationship between RC and atherosclerotic plaques in single and multiple vascular territories.

Mendelian randomization studies on cardiometabolic factors and intracranial aneurysms: A systematic literature analysis.

Objectives: Intracranial aneurysm (IA) has an insidious onset, and once ruptured, it carries high rates of mortality and disability. Cardiometabolic factors may be associated with the formation and rupture of IA. This study aims to summarize the application of Mendelian randomization (MR) methods in research on cardiometabolic factors and IA, providing insights for further elucidation of IA etiology and pathogenesis.

One-pot synthesis of a polydopamine-glued g-CN/CoFeWO membrane for synergistic photocatalytic water purification.

A polydopamine-glued g-CN/CoFeWO membrane, prepared one-pot synthesis, achieves complete sulfamethoxazole degradation through synergistic photocatalysis and PMS activation. It exhibits robust stability over 10 hours of continuous operation, maintaining high efficiency (97%) even in real municipal wastewater effluent, offering a novel and promising water purification strategy.

The helical elongation of π-extended diazahelicenes with amplified circularly polarized luminescence.

Helicenes are circularly polarized luminescence (CPL)-active but suffer from a fundamental tradeoff between fluorescence quantum yield (Φ) and luminescence dissymmetry factor (||). Herein, we present a strategy combining lateral π-extension and helical elongation in carbazole-embedded helicenes to address this challenge. Specifically, π-extended diaza[7]helicene () and diaza[9]helicene () were synthesized and characterized, revealing nearly a 2-fold increase in Φ and a 6-fold enhancement in || from to .

Activity-Based Ubiquitin Probes Capture the Sulfenylated State of Deubiquitinases.

Activity-based ubiquitin probes (Ub-ABPs) are powerful tools for studying the functional landscape of deubiquitinases (DUBs). While most existing Ub probes have focused on examining the native state of DUBs, oxidative stress, especially in cancer and inflammatory contexts, can oxidize the catalytic cysteine of DUBs, significantly altering their activity. Here, we developed three novel ubiquitin-based activity probes (Ub-ABPs) to selectively trap the sulfenylated form of deubiquitinases (DUB-SOH).

Splicing factor Nova regulates the splice variants in exons 3 and 6 of GABA receptor subunit RDL from Chilo suppressalis Walker (Lepidoptera: Crambidae).

The insect ionotropic γ-aminobutyric acid (GABA) receptor is an important insecticide target, and alternative splicing (AS) among exons 3a, 3b, 6a, and 6b of its RDL subunit is ubiquitous in insects; however, the AS factors and mechanisms remain unclear. While the neuro-oncological ventral antigen (Nova) is known to regulate AS of the γ2 subunit of mammalian GABA receptors, its role in insects remains unexplored. Two CsNova isoforms, CsNova-X1 and CsNova-X3, were identified by BLAST in the third-generation transcriptome of Chilo suppressalis.

A strain of mycovirus AaVV1 mediates hypovirulence of Alternaria species.

The genus Alternaria comprises a wide range of ubiquitous plant pathogens that affect various host plants. Certain mycoviruses can induce changes in the biological characteristics and virulence of host fungi, offering potential for biocontrol in managing fungal plant diseases. Here, we identified a mycovirus with a high degree of homology to Alternaria arborescens victorivirus 1 (AaVV1), which was previously reported from Alternaria arborescens, in the QRH strain of the heterologous host Alternaria gomphrenae.

UCP2 is identified as a therapeutic target for abdominal aortic aneurysm by comprehensive bioinformatic analysis and experimental validation.

Abdominal aortic aneurysm (AAA) is a potentially life-threatening vascular condition that currently lacks effective pharmacological treatment. The disease is strongly associated with chronic inflammation, where immune cells like macrophages play a crucial role. Efferocytosis, the process by which apoptotic cells are cleared, is involved in regulating inflammation.

Light-emitting diode-derived blue light overexposure accelerates corneal endothelial cell aging by inducing abnormal ROS accumulation.

Blue light, defined as short-wavelength visible light ranging from 400 to 500 nm, is recognized for its high energy within the visible light spectrum. The prevalent use of light-emitting diodes (LEDs) has significantly increased exposure to blue light. Corneal endothelial cells (CECs) playing a crucial role in maintaining corneal transparency to get clear visual field.

DeepPhosPPI: a deep learning framework with attention-CNN and transformer for predicting phosphorylation effects on protein-protein interactions.

Protein phosphorylation regulates protein function and cellular signaling pathways, and is strongly associated with diseases, including neurodegenerative disorders and cancer. Phosphorylation plays a critical role in regulating protein activity and cellular signaling by modulating protein-protein interactions (PPIs). It alters binding affinities and interaction networks, thereby influencing biological processes and maintaining cellular homeostasis.

PreRBP: Interpretable deep learning for RNA-protein binding site prediction with attention mechanism.

In the complex process of gene expression and regulation, RNA-binding proteins occupy a pivotal position for RNA. Accurate prediction of RNA-protein binding sites can help researchers better understand RNA-binding proteins and their related mechanisms. And prediction techniques based on machine learning algorithms are both cost-effective and efficient in identifying these binding sites.

The renoprotective anti-hyperuricemia effect of Cornus officinalis extract in hyperuricemia rats based on network pharmacology and multiple omics.

Ethnopharmacological Relevance: Corus officinalis Siebold & Zucc belongs to the genus Cornus in the Cornaceae family, and was first recorded in the "Shennong Herbal Classic", now has been included in "according to the tradition of both food and Chinese herbal medicines", consist of kidney and liver tonifying, antioxidant substances including cycloid glycosides, flavonoids, polyphenols, organic acids, etc. AIM OF THE STUDY: This study was aimed at discovering the mechanism underlying the anti-hyperemia effect of Cor in rats, particularly its protective effect against liver and kidney dysfunction caused by HUA.

Materials And Methods: In this study, the effect of Cor extract against HUA was verified in rats, subsequently, network pharmacology combined with non-targeted metabolomic were performed to investigate its composition characteristics, and further multi-omics studies and molecular validation were performed to reveal molecular mechanism both in vivo and in vitro.

Forty years of global research on WHO's four critical priority fungal pathogens: Advances and prospects.

Background: On October 25, 2022, the World Health Organization (WHO) released its first Fungal Priority Pathogen List (FPPL), classifying Cryptococcus neoformans (C. neoformans), Candida auris (C. auris), Aspergillus fumigatus (A.

Beyond top-hit nontarget screening: Diagnostic fragment analysis reveals nitrogen-containing heterocycles in iron and steel industry wastewater.

Nitrogen-containing heterocyclic compounds (NHCs), widely present in industrial wastewater, pose significant environmental and health risks, yet their identification and characterization remain poorly understood. Herein, we developed a diagnostic fragment list comprising 20 nitrogen-containing fragments for NHCs, by integrating chemical information from Pubchem with the NIST mass spectral library. Leveraging this list, we employed a diagnostic fragment-assisted nontarget screening approach and identified 151 NHCs in iron and steel industry wastewater.

Geographical flavor fingerprinting of morels: integrated analysis of volatile and non-volatile components with environmental drivers across Chinese regions.

Morel (Morchella spp.) is highly valued and increasingly widely cultivated in China. However, due to the limited analysis of its flavor components and origin, its full utilization is restricted.

Association of the PWWP-domain protein HUA2 and the H3K36 methylation in flowering time control.

Trimethylation of histone H3 at lys36 (H3K36me3) promotes gene transcription and governs plant development and plant responses to environmental cues. Yet, how H3K36me3 is translated into specific downstream events remains largely uninvestigated. Here, we report that the Arabidopsis PWWP-domain protein HUA2 binds methyl-H3K36 in a PWWP motif-dependent manner.

Synthesis and Biological Evaluation of Platensimycin Analogues with Improved Antimycobacterial Activity.

KasA and KasB are promising drug targets against and infectious nontuberculous mycobacteria, while most lead compounds are in the preclinical development stage. Herein, a platensimycin (PTM) analogue library consisting of 340 members was first screened to identify 46 PTM thioethers with superior activity compared to that of PTM against . Next, 19 PTM thioethers were chosen and semisynthesized from PTM oxirane (), together with seven PTM ether derivatives and 6-ido, 6-bromo-, and 6-thiocyanato PTM.

T-2 Toxin Exploits Gut-Derived Staphylococcus Saprophyticus to Disrupt Hepatic Macrophage Homeostasis.

T-2 toxin, a mycotoxin that frequently causes hidden contamination in food and animal feed, poses a substantial threat to both human and animal health. Staphylococcus saprophyticus (S. saprophyticus) is an opportunistic pathogen that widely infects humans and various animals.

Connexin43 and Its Regulation of Astrocyte Gap Junction Function: Influencing Depression Progression by Mediating Electrical and Chemical Signals.

Background: Depression is a common mental illness with a high relapse rate, which has a serious negative impact on national economic development and happiness. At present, the pathogenesis of depression is still unclear, and there are inevitable limitations in first-line clinical treatment. Therefore, it is very important to clarify the pathological mechanism of depression for the development of safe and effective antidepressants.

Integration of humanized ROBO1 CAR in PD-1 locus in natural killer cells delivers synergistic tumor-killing effect against non-small cell lung cancer.

Lung cancer is the most common cancer and one of the leading causes of cancer-related deaths in the world, however, the treatment of non-small cell lung cancer (NSCLC) is still limited, and it is a clinically urgent problem. ROBO1 is an important surface receptor on tumor cells, but the role of humanized chimeric antigen receptor (CAR) modified natural killer (NK) cells targeting ROBO1 in NSCLC is rarely explored. Furthermore, the role of PD-1 in NK cell killing tumor cells remains controversial.

Naturally impaired side-chain shortening of aromatic 3-ketoacyl-CoAs reveals the biosynthetic pathway of plant acetophenones.

Acetophenones, which show scattered distribution across phylogenetically distant plants and fungi, play diverse roles in plant-plant, plant-insect, plant-microbiome and even animal-insect interactions. However, the enzymatic basis of acetophenone biosynthesis in plants remains unknown. Here we elucidate the complete biosynthetic pathway of picein (4-hydroxyacetophenone glucoside) from 4-coumaroyl-CoA using pear (Pyrus) as a study system.

Machine learning for the prediction of blood transfusion risk during or after mitral valve surgery: a multicenter retrospective cohort study.

This study aimed to identify the optimal prediction method and key preoperative variables for red blood cell (RBC) transfusion risk in patients undergoing mitral valve surgery. We conducted a retrospective study involving 1477 patients from eight large tertiary hospitals in China who underwent mitral valve surgery with cardiopulmonary bypass. From thirty collected preoperative variables, the Max-Relevance and Min-Redundancy (mRMR) method was used for feature selection, and various machine learning models were evaluated.

Nanocellulose-assisted construction of conductive gradient hydrogel for remote actuated and self-sensing soft actuator.

Hydrogel actuators show tremendous promise for applications in soft robots and artificial muscles. Nevertheless, developing a stretchable hydrogel actuator combining remote actuation and real-time signal feedback remains a challenge. Herein, a light-responsive hydrogel actuator with self-sensing function is fabricated by employing a localized immersion strategy to incorporate polyacrylamide (PAM) hydrogel network into semi-interpenetrating carbon nanotube/2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized cellulose nanofiber/poly(N-isopropylacrylamide) (CNT/TOCN/PNIPAM) hydrogel.

Predictors of progression from pre-frailty to frailty in older people: a systematic review and meta-analysis protocol.

Introduction: Frailty is a global health issue, particularly among older adults, and is strongly associated with adverse health outcomes. The intermediate stage of pre-frailty, which represents a transition from robust health to frailty, has garnered growing concern due to its potential reversibility. This systematic review and meta-analysis will aim to identify predictors associated with the progression from pre-frailty to frailty in older adults.

STAT1-VAMP8 axis drives nasopharyngeal carcinoma progression via autophagy enhancement.

Nasopharyngeal carcinoma (NPC) is a malignant tumor with a high risk of invasion and metastasis. Elucidating the molecular underpinnings of NPC may uncover new diagnostic and therapeutic targets. Vesicle associated membrane protein 8 (VAMP8) is overexpressed and plays an oncogenic role in various tumors.