Dual-ferroptosis induction-based microneedle patches for enhanced chemodynamic/photothermal combination therapy against triple-negative breast cancer.

Triple-negative breast cancer (TNBC) remains a refractory subtype of breast cancer due to its resistance to various therapeutic strategies. In this study, we introduce a "brake-release and accelerator-pressing" approach to engineer a microneedle patch embedded with copper-doped Prussian blue nanoparticles (Cu-PB) and the ferroptosis inducer sorafenib (SRF) for raised chemodynamic (CDT)/photothermal (PTT) combination therapy against TNBC. Upon transdermal insertion, the dissolving microneedles swiftly disintegrate and facilitate the release of SRF.

Cysteine mediates the association of essential trace elements with polycystic ovary syndrome: Does ambient particulate matters exposure modify the mediation effect of cysteine.

Objective: In this study, we aimed to investigate the relationship between essential trace element levels in FF and PCOS and to determine whether this association was mediated by the antioxidant stress indicator cysteine (Cys). In addition, the effect of ambient PM exposure on the mediation effect of Cys was examined.

Methods: A case-control study was conducted, which involved 420 patients with PCOS and 408 controls.

Melatonin Protects against LPS-Induced Mitochondrial Dyshomeostasis and Ovarian Damage through JNK Signaling Pathway in Mouse Ovary.

Both mitochondrial dysfunction and inflammation are closely associated with the pathogenesis of diminished ovarian reserve (DOR). While melatonin (MT) is known to protect against ovarian injury, its precise mechanism in counteracting lipopolysaccharide (LPS)-induced mitochondrial dysfunction and ovarian reserve impairment remains unclear. This study aimed to explore the effects and underlying mechanisms of MT on LPS-induced ovarian reserve dysfunction.

A missense mutation in TCFL5 is associated with male infertility due to oligoasthenoteratozoospermia.

Oligoasthenoteratozoospermia (OAT) is a prevalent situation of male infertility partly caused by genetic defects with largely undiscovered. To further unravel the genetic etiology of OAT, we recruited cases for whole-exome sequencing (WES) to screen candidate pathogenic mutations. Here, we identified a heterozygous missense mutation in transcription factor-like 5 (TCFL5) (NM_006602.

Gelatin Methacryloyl Xerogel Puncture Implants Loaded with CuMnO Nanoparticles Synergizes Cuproptosis and STING Activation for Enhanced Breast Cancer Immunotherapy.

The immunosuppressive niche of the breast cancer microenvironment and the vascular basement membrane significantly hinder the efficacy of nanoparticle-based antitumor immunotherapy delivered via the vascular system. This study describes the design of a puncture-delivered tumor implant, utilizing a photo-cross-linked gelatin methacryloyl hydrogel as the matrix, loaded with CuMnO nanoparticles (CMO NPs) and monomethyl fumarate (MMF), enabling the sustained release of nanoparticles through matrix metalloproteinase-responsive degradation. Mechanistically, CMO NPs induce cuproptosis and immunogenic cell death (ICD) in tumor cells, while synergizing with MMF to trigger substantial mtDNA release into the cytosol.

Comprehensive management of diabetic ulceration: strategies and perspectives.

Diabetic Ulceration (DU) is a main category of nonhealing chronic wounds and tends to be vulnerable and ulcerated repeatedly. Prolonged hyperglycemia microenvironment, together with immunochemotactic response dysfunction, eventually contributes to the refractory wound that is hard to heal. In the chronic DU, there exist obstacles such as diabetic wound bacterial infection, persistent inflammation, suppressed angiogenesis, and reduced growth factor proliferation with decreased hypoxia inducible factor 1 alpha (HIF-1α) expression, and these issues have persisted in hindering the rational and effective wound tissue regeneration.

Preconception paternal exposure to zearalenone impairs male fertility and IVF embryo survival in mice.

Zearalenone (ZEA), a fungal mycotoxin commonly found in various human foods, exhibits a structural similarity to naturally occurring estrogen, raising significant concerns regarding its reproductive toxicity. This study aimed to assess the impact of preconceptional ZEA exposure on male reproductive health and early embryonic development. Healthy B6D2F1 male mice were exposed to corn oil or varying doses of ZEA for two weeks.

Nucleic acid-based nanogels for drug delivery：From construction strategies to intelligent applications.

Nanogels are nanoscale hydrogel particles, formed through intramolecular or intermolecular cross-linking. They serve as versatile carriers in drug delivery due to their high stability, excellent biocompatibility, and intelligent responsiveness to environmental stimuli. Nucleic acid-based nanogels are of particular interest due to the unique characteristics of nucleic acids.

Subgenual anterior cingulate cortex functional connectivity abnormalities in depression: insights from brain imaging big data and precision-guided personalized intervention via transcranial magnetic stimulation.

The subgenual anterior cingulate cortex (sgACC) plays a central role in the pathophysiology of major depressive disorder (MDD). Its functional interactive profile with the left dorsal lateral prefrontal cortex (DLPFC) is associated with transcranial magnetic stimulation (TMS) treatment outcomes. Previous research on sgACC functional connectivity (FC) in MDD has yielded inconsistent results, partly due to small sample sizes and limited statistical power.

Multi-omics analyses of the gut microbiome, fecal metabolome, and multimodal brain MRI reveal the role of and its related metabolites in major depressive disorder.

Background: Compelling evidence claims that gut microbial dysbiosis may be causally associated with major depressive disorder (MDD), with a particular focus on However, little is known about the potential microbiota-gut-brain axis mechanisms by which exerts its pathogenic effects in MDD.

Methods: We collected data from 16S rDNA amplicon sequencing, untargeted metabolomics, and multimodal brain magnetic resonance imaging from 111 MDD patients and 102 healthy controls. We used multistage linked analyses, including group comparisons, correlation analyses, and mediation analyses, to explore the relationships between the gut microbiome (), fecal metabolome, brain imaging, and behaviors in MDD.

Disrupted resting-state interhemispheric functional connectivity in patients with first-episode moyamoya disease.

Moyamoya disease (MMD) has been reported to be associated with a wide range of structural and functional abnormalities of bilateral hemispheres. However, whether the interhemisphere functional connectivity (FC) of MMD patients is altered and its relationship with cognitive impairments still remain unclear. A total of 20 patients with MMD and 25 healthy subjects were included, matched based on age, sex, and level of education.

Bioactive siRNA-Based Liposomes Promoted Tendon-Bone Healing in Osteoporotic Mice by Recovering the Stemness of CD248 TSPCs.

Osteoporosis significantly impairs tendon-bone healing, increasing the risk of rotator cuff tears and postoperative retearing. Tendon stem/progenitor cells (TSPCs) are vital for tendon repair, with their stemness crucial to healing outcomes. This study investigated the role of CD248 in regulating TSPC stemness and assessed the therapeutic potential of si-CD248-loaded liposomes in promoting tendon-bone healing under osteoporotic conditions.

Multifunctional microneedle-mediated photothermo-gas-ion synergic therapy accelerates MRSA infacted diabetic wound healing.

Chronically high blood glucose levels in diabetic wounds form a complex microenvironment, and this microenvironment is represented by hypoxia, infection, and inflammation. Such an environment significantly impedes the wound healing cascade. Accordingly, monotherapeutic approaches have proven inadequate in addressing the complex nature of these wounds.

Epigallocatechin gallate cooperated with hydrogel encapsulation enables high-performance cryopreservation of mouse ovaries.

Cryopreservation and transplantation of ovarian tissue have emerged as promising techniques for preserving fertility in prepubescent girls and cancer patients who cannot postpone treatment. However, this technology faces several challenges. In particular, conventional methods often fail to mitigate serious oxidative stress issues and cytotoxic damage caused by cryoprotectants, which can affect transplant outcomes.

Event-related potentials reveal hypnotherapy's impact on attention bias in social anxiety disorder.

Background: Exploring hypnotherapy's potential to modulate attention bias offers promising avenues for treating social anxiety disorder (SAD).

Aim: To determine if hypnotherapy can alleviate social anxiety by influencing attention bias, specifically identifying the aspects of attention processes affected by hypnosis.

Methods: In this study, 69 SAD participants were divided into three groups based on their Liebowitz Social Anxiety Scale scores: Experimental group, control group, and baseline group.

Associations between the meniscal lesion parameters and knee joint structures and symptoms in patients with symptomatic knee osteoarthritis.

Background: To investigate cross-sectional associations between meniscal lesion parameters and knee joint structures and symptoms in patients with symptomatic knee osteoarthritis (KOA).

Methods: A total of 102 subjects with symptomatic KOA were included in this study. Coronal magnetic resonance imaging was used to measure the meniscal parameters including the mean distance from medial to lateral meniscal lesions (Mean(MLD)), the mean value of tibial plateau width (Mean(TPW)) and the mean of the relative percentage of the medial to lateral meniscal lesions distance (Mean(RMLD)).

Hyaluronic acid microneedle patch for transdermal delivery of frankincense and myrrh bioactives to alleviate inflammation and enhance diabetic ulcer healing.

Chronic inflammation impedes the healing of diabetic ulcers (DUs), while P-glycoprotein (P-gp)-mediated drug efflux reduces the efficacy of conventional drug formulations. In this study, a novel liposome-mediated hyaluronic acid (HA) microneedle patch (E-Lipo@A-HMN) was developed for enhanced DUs healing via the transdermal delivery of Acetyl-11-keto-β-boswellic acid (AKBA) and β-elemene (ELE). The findings demonstrate that liposomal encapsulation enhances the solubility and bioavailability of ELE, and HA microneedles improve skin penetration and enable controlled drug release by providing a biodegradable, biocompatible scaffold that improves skin penetration and enables controlled drug release.

Venetoclax synergizes with Wnt/β-catenin inhibitor C-82 in acute myeloid leukemia by increasing the degradation of Mcl-1 protein.

Background: Due to compensatory survival signalling and overexpression of anti-apoptotic Bcl-2 family proteins, the majority of AML patients developed acquired resistance of venetoclax (VEN) to combination therapy of VEN with low-dose cytarabine (LDAC) or hypomethylating agents (HMAs). Dysregulation of the Wnt/β-catenin signalling pathway is intently associated with leukemia development and chemotherapy resistance. However, there is currently no Wnt/β-catenin inhibitor approved for clinical use and it is not clear whether targeting the Wnt/β-catenin pathway enhances the anti-leukemic activity of VEN.

Analysis of emotional fluctuations in infertile women during ART treatment: the impact of ovarian hyperstimulation.

Background: Infertility affects many reproductive-aged couples, with assisted reproductive technology (ART) offering hope but also significant emotional stress due to invasive procedures, hormonal fluctuations, uncertainty, and financial and emotional burden of repeated cycles. Ovarian hyperstimulation syndrome (OHSS), a common ART complication, may further aggravate psychological distress, yet its mental health impact remains understudied.

Methods: This longitudinal study included 1,429 women undergoing their first ART cycle at a reproductive center in China.

Validating the Chinese version of the Apathy Motivation Index and network analysis of apathy subtypes in a healthy Chinese sample.

Apathy is a neuropsychiatric syndrome associated with various negative outcomes in patients with motivational disorders, commonly examined in multiple dimensions including cognitive, behavioral, and emotional domains. While there are some tools to measure apathy in China, there is a lack of appropriate instruments specifically designed to assess social motivation for a more comprehensive definition of apathy. Moreover, there is limited research on the complex interrelations among the intrinsic domains of apathy.

Rapid diagnosis of diquat and paraquat poisoning: A dual approach using colloidal gold immunochromatographic assay and hydrophilic interaction liquid chromatography.

Accurate diagnosis and treatment in poisoning cases necessitate the monitoring of diquat and paraquat concentrations. This study presents a competitive colloidal gold immunochromatographic assay (GICA) strip for qualitative detection, alongside hydrophilic interaction liquid chromatography with ultraviolet detection (HILIC-UV) for quantitative assessment. Both methods underwent rigorous validation.

Genetic mechanisms underlying gray matter atrophy in Parkinson's disease: a combined transcriptome and neuroimaging study.

Extensive studies have demonstrated significant gray matter atrophy in patients with Parkinson's disease (PD); however, the underlying gene expression mechanisms remain largely unknown. To comprehensively characterize the gray matter volume alterations in PD patients, we conducted a neuroimaging meta-analysis and validated the observed atrophic phenotypes in an independent dataset. Leveraging the Allen Human Brain Atlas (AHBA), we linked brain transcriptomic data to neuroimaging phenotypes to identify genes associated with PD-related gray matter atrophy.

Deciphering the profiles and hosts of antibiotic resistance genes and evaluating the risk assessment of general and non-general hospital wastewater by metagenomic sequencing.

Hospital wastewater (HWW) is a substantial environmental reservoir of antibiotic resistance genes (ARGs) and poses risks to public health and aquatic ecosystems. However, research on the diversity, transmission mechanisms, pathogenic hosts, and risks of ARGs in different HWW types is limited. This study involved the collection of HWW samples from 15 hospitals in Hefei, China, which were subsequently categorized as general hospitals (GHs) and non-general hospitals (NGHs).

Disrupted interhemispheric functional and structural connectivity in patients with major depressive disorder.

Background: Major depressive disorder (MDD) is associated with disrupted interhemispheric cooperation. However, the relationship between structural and functional alterations in interhemispheric cooperation in patients with MDD remains unclear. We investigated the associations between voxel-mirrored homotopic connectivity (VMHC) and radial diffusivity (RD) within the corpus callosum (CC) and their links to depressive symptoms in patients with MDD.

Orally Administered ZnCeO/Se Hydrogel with Effective Antioxidant Activity for Treating Inflammatory Bowel Disease by Inhibiting Ferroptosis.

Oxidative stress leads to intestinal barrier damage, which induces immune responses to occur and further promotes oxidative stress exacerbating inflammatory bowel disease (IBD). In this work, the multifunctional ZnCeO/Se (ZCSO) nanozyme wrapped with acid-resistant calcium alginate hydrogel designed for oral administration is prepared. The ZCSO nanozyme can promote the activation of the Nrf2 oxidative stress pathway, then significantly improve the efficiency of scavenging reactive oxygen species (ROS) and up-regulate the protein expression of glutathione peroxidase 4 (GPx4), which is closely related to the inhibition of ferroptosis.