Dnmt1 Alleviates S1PR1-Mediated Pyroptosis after Spinal Cord Injury through Regulating Pon3 Expression.

Spinal cord injury (SCI), as a severe neurological disorder, remains a formidable challenge in clinical treatment. Pyroptosis-triggered neuroinflammation exacerbates secondary damage, neuronal death, and impairs recovery after SCI, making it a critical pathological factor. However, the exact pathophysiological mechanisms are incompletely understood.

The WRKY transcription factor RsWRKY28 mediates cadmium extrusion via activating RsPDR8 expression in radish (Raphanus sativus L.).

Cadmium (Cd) is a widespread dangerous pollutant for its toxic effects on plants and human beings. The uptake and accumulation of Cd from soil impedes taproot enlargement, thereby reducing yield, quality and safety of radish taproot. However, the molecular mechanisms and regulatory networks underlying Cd accumulation in radish remain unclear.

Microglial NFAT5 aggravates neuroinflammation via mediating NLRP6 inflammasome in experimental ischemic stroke.

Microglial activation triggers the inflammatory cascade and exacerbates brain injury following ischemic stroke. Middle cerebral artery occlusion (MCAO) modeling increased the expression of nuclear factor of activated T cells 5 (NFAT5) in microglia. However, the role of microglial NFAT5 in ischemic stroke remains unclear.

Growth Factor Receptor-Binding Protein 10 Promotes High Glucose-Induced H9c2 Cardiomyoblast Apoptosis via Inhibition of the ERK1/2 and JAK2/STAT3 Signaling Pathways.

Cardiomyocyte apoptosis is crucial in the occurrence of diabetic cardiomyopathy; thus, it is important to elucidate the underlying mechanisms involved in elevated glucose and cardiomyocyte apoptosis. Growth factor receptor-binding protein 10 (Grb10) has been proved to participate in the regulation of cell proliferation, migration, and apoptosis. The purpose of this study was to explore the role of Grb10 in high glucose-induced H9c2 cardiomyoblast apoptosis and investigate the underlying molecular mechanisms.

Automated annotation of complex natural products using a modular fragmentation-based structure assembly (MFSA) strategy.

Complex natural products (CNPs) feature polycyclic structures, multiple consecutive chiral centers, and nonrepetitive structural units. Given their complexity, the structural annotation of CNPs remains a major bottleneck. Here, we introduce the modular fragmentation-based structural assembly (MFSA) strategy for the target CNP structural annotation.

mRNA-Based FRET-FLIM Imaging Platform for Quantifying Lipid Nanoparticle Endosomal Escape and Membrane Damage.

Efficient endosomal escape and membrane damage are crucial for mRNA-lipid nanoparticle (LNPs) delivery but remain challenging to quantify. Here, we develop FRET-lifetime imaging for mRNA-LNP tracking (FLINT), an intramolecular FRET-FLIM platform to dynamically monitor mRNA release kinetics and endosomal damage in live cells. Capitalizing on the 50- to 100-fold higher concentration of glutathione (GSH) in the cytosol relative to endocytic vesicles, the FLINT employs disulfide-linked fluorophores conjugated to mRNA uridines, enabling precise tracking of cytosolic mRNA via GSH-activated signal amplification (20-50×).

GA suppresses the conversion from α-linolenic acid to JA through the GhGAI1-GhMYC3-GhLOX3 module to promote cotton fiber elongation.

Cotton fibers are essential raw materials for the textile industry and serve as a classical model for studying cell elongation. During the elongation phase, cotton fibers accumulate significant amounts of linolenic acid, which is an essential component of cell membranes and a key precursor for jasmonic acid (JA) biosynthesis. Although JA is known to play a critical role in fiber development, the regulatory mechanisms governing the conversion of linolenic acid to JA remain largely unknown.

Risk factors, protective factors, peripheral biomarkers, and neurocognitive markers associated with mood disorders: An umbrella review of 103 meta-analyses and systematic reviews.

Background: Mood disorders, including bipolar disorder (BD) and major depressive disorder (MDD), are extensively studied regarding environmental risk factors, protective factors, peripheral biomarkers, and cognitive neurocognitive markers. Our umbrella review of observational studies aims to separately elucidate these associations with BD and MDD.

Methods: A comprehensive search of PubMed, Embase, Web of Science, and Cochrane was conducted, with a search end date of October 17, 2024.

Binuclear Metal-Based Covalent Organic Framework Mimicking Metallohydrolases for Direct Photoreforming of PET Plastic.

Direct photoreforming of waste poly(ethylene terephthalate) (PET) plastic into high-value-added chemicals is a promising method that improves resource utilization. However, the traditional method for PET depolymerization was mostly harsh alkali pretreatment (C = 5-10 M), which largely impeded direct reforming due to the diversity of the conditions and thus caused inefficiency and discontinuity for plastic upgrading. Binuclear metallohydrolases have been proven to cause maximal acceleration of hydrolytic processes via the proximity effect under mild conditions but are unable to realize further reforming reaction due to monofunctionality.

Integrated Multi-Omics Profiling Reveals That Highly Pyroptotic MDMs Contribute to Psoriasis Progression Through CXCL16.

Psoriasis, an inflammatory skin disorder, involves pyroptosis-a pro-inflammatory cell death process. However, cell-specific pyroptosis dynamics and immune microenvironment interactions remain unclear. To investigate cell-type-specific pyroptosis patterns in psoriasis and their immunoregulatory mechanisms.

Unidirectional Crosstalk Between NTRK1 and IGF2 Drives ER Stress in Chronic Pain.

: Chronic postsurgical pain (CPSP) poses a major clinical challenge due to unresolved links between neurotrophic pathways and endoplasmic reticulum (ER) stress. While Neurotrophic Tyrosine Kinase Receptor Type 1 (NTRK1) modulates ER stress in neuropathic pain, its interaction with Insulin-Like Growth Factor II (IGF2) in CPSP remains uncharacterized, impeding targeted therapy. This study defined the spinal NTRK1-IGF2-ER stress axis in CPSP.

Suhexiang pills inhibit ischemia stroke via targeting miR-24-3p to promote the activation of Keap1/Nrf2 signaling pathway.

The incidence of ischemic stroke (IS) is increasing year by year in China and the world. Cerebral ischemia-reperfusion injury (CIRI) caused by blood flow restoration during treatment seriously affects the prognosis of the disease. Suhexiang pills (ShxP) is a traditional Chinese medicine prescription for the treatment of IS.

Deciphering the differential impact of CDK4 mutations on abemaciclib binding in oral squamous cell carcinoma: a precision medicine approach.

Background: Precisi on medicine is an emerging approach for disease treatment and its major challenge is the development of drug resistance. Understanding the role of specific mutations in drug resistance is crucial for the development of next-generation therapeutics.

Methodology: Cyclin-dependent kinase-4 (CDK4) is a pivotal regulator of the cell cycle, with mutations implicated in oncogenesis in oral squamous cell carcinoma (OSCC).

Evidence Mapping of Clinical Practice Guidelines Recommendations and Quality for Nutritional Management in Dementia.

Aim: This study aimed to systematically review Clinical Practice Guidelines (CPGs) for nutritional management of dementia and use evidence mapping to highlight research trends and identify gaps to inform future research.

Design: A systematic review of guidelines using the PRISMA statement.

Methods: Systematically collect literature on dementia management CPGs from PubMed, Embase, Web of Science and guideline databases.

Mapping Dermatology Life Quality Index with EQ-5D health utility index score in Chinese patients with moderate to severe psoriasis.

To establish correlation between Dermatology Life Quality Index (DLQI) and EuroQol 5-Dimension questionnaire (EQ-5D) utility score in Chinese moderate-to severe psoriasis patients and develop mapping models for health utility prediction. A total of 287 patients with moderate to severe psoriasis and at least one clinical visit with assessments for both DLQI and EQ-5D in a Chinese tertiary hospital were included. These patients were randomly split into a training set (n = 231) and a testing set (n = 56).

Virtual Screening of Novel Eco-Friendly Gaseous Dielectrics through Dimensionless Bond Decomposition and Machine Learning Algorithm.

Identification of environmentally friendly gaseous dielectrics to replace the most potent greenhouse gas SF is urgently desired in the worldwide high-voltage electrical industry. However, the great challenge for SF-free technology remains because of numerous contradictory requirements it has to meet simultaneously: high dielectric strength, low boiling points, low global warming potential, high arc quenching capability, low acute/subchronic inhalation toxicity, and low flammability. Herein, the chemical bonds are revealed to be the universal, unique, and unified descriptors to develop the predictive models for efficient virtual screening of novel gaseous dielectrics.

Microglial exosome TREM2 ameliorates ferroptosis and neuroinflammation in alzheimer's disease by activating the Wnt/β-catenin signaling.

Microglia and exosomes are intimately connected with the pathogenesis of Alzheimer's disease (AD). We aim to investigate the role and potential mechanisms of M2-like (anti-inflammatory) microglia-derived exosomes (M2-Exos) in AD. We utilized an Aβ-induced AD model in HT-22 neurons and mouse.

Indole Alkaloids and Phenolic Amides from the Rhizomes of and Their In Vitro Soluble Epoxide Hydrolase (sEH) Inhibitory Activity.

is a perennial herb that belongs to the Ranunculaceae family. Its dried rhizomes are a source of Cimicifugae Rhizoma, a traditional Chinese medicine used for detoxification, the treatment of febrile diseases, and the prevention of pathogenic invasion. In the present study, a phytochemical investigation of the rhizomes of resulted in the isolation of three indole alkaloids (-) and seven phenolic amides (-), including three new compounds, 6-methoxy-3-(3-methyl-1-oxo-2-butenyl) 1 indole (), (3)-1-(-d-glucopyranosyl)-3-hydroxy-3-(3-methylbutyl)-2-oxindole (), and -acetyldopamine-3---d-allopyranoside ().

All-cause mortality in patients with medullary thyroid carcinoma of different ages: an inverse L-curve analysis study.

Background: Medullary thyroid carcinoma (MTC) is a malignancy with a high mortality rate and a wide age range. However, there are relatively few studies on the relationship between age and all-cause mortality in patients with MTC. As one of the important factors influencing cancer prognosis, the association between age and all-cause mortality in MTC patients needs to be further investigated.

The differential role of spousal collaboration on pain reactivity to daily stressors in older couples with and without multiple chronic conditions.

Background: Spousal collaboration has been considered as important coping resource to deal with aging-related health problems, but little is known about the role of spousal collaboration on the everyday stress-reactivity process in couples facing multiple chronic conditions (MCCs).

Purpose: We examined the within-person association of daily stressors and pain, and whether spousal collaboration played a differential role in moderating this association in older couples living with and without MCCs.

Methods: Data were drawn from an ecological momentary assessment (EMA) research, wherein two sub-samples of older married couples (including 52 dyads facing couple-level MCCs and 55 dyads without couple-level MCCs) reported 7671 (89.

Event-related potentials reveal hypnotherapy's impact on attention bias in social anxiety disorder.

Background: Exploring hypnotherapy's potential to modulate attention bias offers promising avenues for treating social anxiety disorder (SAD).

Aim: To determine if hypnotherapy can alleviate social anxiety by influencing attention bias, specifically identifying the aspects of attention processes affected by hypnosis.

Methods: In this study, 69 SAD participants were divided into three groups based on their Liebowitz Social Anxiety Scale scores: Experimental group, control group, and baseline group.

Global research trends in gastroenteropancreatic neuroendocrine tumors: a bibliometric analysis from 2000 to 2023.

Introduction: The incidence of gastroenteropancreatic neuroendocrine tumors (GEP-NETs) is increasing. To improve patient outcomes, it is essential to develop integrated treatment strategies based on tumor characteristics. Researchers need a rapid visualization of global research trends in GEP-NETs.

BUBR1 Facilitated Nerve Regeneration by Modulating STAT3 Phosphorylation, Thereby Alleviating HIE in Neonatal Mice.

Hypoxic-ischemic encephalopathy (HIE) represents a leading cause of morbidity and mortality among neonates. However, the underlying molecular mechanisms of HIE remain largely elusive. A HIE animal model was established, and neural stem cells (NSCs) underwent oxygen-glucose deprivation/reoxygenation (OGD/R) to mimic HIE in vitro.

Reticular Synthesis of 3D Metal Cluster-Based COFs With Record High-Connectivity for Efficient Photocatalytic HO Synthesis.

The assembly of metal clusters with organic building units by covalent bonds to form metal cluster-based covalent-organic frameworks (MCCOFs) material is a novel strategy in reticular synthesis. However, only 2, 3, and 6 connectivity of MCCOFs have been achieved in reported structures. Developing a higher connectivity remains a great challenge in the MCCOFs due to the difficulties in introducing multiple connecting nodes to the metal cluster.

Global and regional disease burden of HIV/AIDS from 1990 to 2021 and projections to 2030.

Background: Few studies have focused on the descriptive epidemiology and trends of HIV/AIDS since the publication of the Global Burden of Disease (GBD) study in 2021. Therefore, this study aims to analyze the trends and patterns of the HIV/AIDS burden at the global, regional, and national levels by sex, age and social development index (SDI). We also explored risk factors and predicted the GBD of HIV/AIDS until 2030.