Acupuncture for Rapid Pain Relief and Restoration of Motor Function in Acute Lateral Ankle Sprains: A Randomized Controlled Trial Protocol Based on Infrared Thermography.

Purpose: Acute lateral ankle sprain (ALAS) is a prevalent clinical sports injury disease, rapid relief of symptoms and return to normal functional activity are urgent needs of ALAS patients. The aim of this study is to observe the immediate therapeutic effect of acupuncture on ALAS patients in the short term and to evaluate the efficacy at different time points through a multidimensional assessment system, which is expected to provide a simple, rapid, safe and effective therapeutic option for ALAS patients.

Patients And Methods: This is a single-center, prospective, randomized clinical trial.

Exploring omics strategies for drug discovery from isolated from the marine ecosystem.

Marine are prolific producers of diverse bioactive secondary metabolites, making them vital for drug discovery. Traditional cultivation and bioassay-guided isolation techniques often lead to the rediscovery of the same compounds, revealing the limitations of these traditional approaches and emphasizing the need for more advanced methods. The emergence of omics technologies such as genomics, metagenomics, transcriptomics, and metabolomics has dramatically enhanced the ability to investigate microorganisms by providing detailed insights into their biosynthetic gene clusters, metabolic pathways, and regulatory mechanisms.

Defensive tolerance drives the reprogramming and dysfunction of infiltrating pathogenic B cells assuring the maintenance of tolerance.

We previously showed that infiltrating cytotoxic immune cells are reprogrammed to regulatory-like/exhausted cells within accepted kidney allografts through a 'defensive tolerance' mechanism. We observed a regulatory B cell (Breg) signature within the accepted kidney. Here we show that despite a Breg phenotype, neither B cell depletion nor the use of μMT recipients which lack B cells, resulted in kidney allograft rejection.

Downregulation of ubiquitous microRNA-320 in hepatocytes triggers RFX1-mediated FGF1 suppression to accelerate MASH progression.

Metabolic dysfunction-associated steatohepatitis (MASH), a severe type of metabolic dysfunction-associated steatotic liver disease (MASLD), is a leading etiology of end-stage liver disease worldwide, posing significant health and economic burdens. microRNA-320 (miR-320), a ubiquitously expressed and evolutionarily conserved miRNA, has been reported to regulate lipid metabolism; however, whether and how miR-320 affects MASH development remains unclear. By performing miR-320 hybridization with RNAscope, we observed a notable downregulation of miR-320 in hepatocytes during MASH, correlating with disease severity.

Mobilome-mediated transcriptional activation of biosynthetic gene clusters and its impact on strain competitiveness in food fermentation microbiomes.

Background: Microbial interactions are critical for maintaining the stability of food fermentation microbiomes, and mobile genetic elements (MGEs) significantly influence these interactions by horizontal gene transfer events. Although MGEs are known to facilitate horizontal gene transfer, their distribution among microorganisms and specific effects on microbial interactions remain poorly understood.

Results: We analyzed 590 metagenomic and 42 metatranscriptomic samples from food fermentations, recovering 1133 metagenome-assembled genomes (MAGs).

The association between healthy lifestyle trajectory and depressive symptoms: Findings from the Chinese Longitudinal Healthy Longevity Survey (CLHLS).

Objective: Depression places a significant burden on older people, and some studies have found that certain lifestyles are associated with depressive symptoms. This study aimed to observe the relationship between integrated lifestyles and depressive symptoms from the perspective of dynamic change.

Method: The data for this study comes from the China Health and Longevity Longitudinal Survey (CLHLS) conducted in three waves in 2011-2012, 2014, and 2017-2018.

Tolerogenic Lung Allograft Microenvironment Suppresses Pathogenic Tissue Remodeling Following Respiratory Virus Infection in Mice.

Tolerance after lung transplantation is associated with the induction of Foxp3 regulatory T cell-enriched bronchus-associated lymphoid tissue, which suppresses local and systemic alloimmune responses. How this tolerogenic graft environment shapes responses to respiratory viral infections, a known contributor to adverse outcomes after lung transplantation, remains unknown. Using a mouse model of a seasonally circulating parainfluenza virus, we found that acute infection of tolerant lung allografts results in temporary reductions of both bronchus-associated lymphoid tissue size and abundance of graft-resident Foxp3 cells, but doesn't trigger rejection.

Altererythrobacter guangdongensis sp. nov. and Tsuneonella sediminis sp. nov., two novel carotenoid-producing species isolated from tidal flat sediment.

Four aerobic, Gram-stain-negative, rod-shaped, and non-motile bacterial strains, designated SYSU HZ0097ᵀ, SYSU HZ0136, SYSU LHT278ᵀ, and SYSU LHT272, were isolated from tidal flat sediment collected in Zhuhai, China. Phylogenetic analysis based on 16S rRNA gene sequences assigned all four strains to the family Erythrobacteraceae. Strains SYSU HZ0097ᵀ and SYSU HZ0136 showed the highest 16S rRNA gene sequence similarity (98.

The ethnomedicinal, pharmacological, and phytochemical potential of Ferula sinkiangensis K.M.Shen: An updated and comprehensive review.

Ethnopharmacological Relevance: Ferula sinkiangensis, commonly known as "Chinese Awei" or "Chinese Ferula", has attracted considerable attention due to its diverse ethnobotanical uses and phytochemical complexity. However, lacking an updated and comprehensive review addressing ethnobotany, phytochemistry, pharmacology, and threats facing this critically endangered medicinal plant of Xinjiang, China.

Aim: This comprehensive review aims to provide an updated, meticulous study of F.

Phantom-based assessment of focused ultrasound thermal effects with conventional magnetic resonance imaging.

Background And Objective: This study presents key findings from Magnetic Resonance Imaging (MRI)-guided Focused Ultrasound (FUS) sonication experiments in a specialized gel phantom, aimed at demonstrating the effectiveness of using conventional T1-Weighted (T1-W) and T2-Weighted (T2-W) Turbo Spin Echo (TSE) sequences to assess FUS thermal effects and related system performance.

Methods: Three custom-manufactured, single-element spherically focused ultrasonic transducers were utilized in this study. The temporal regression of lesions induced by high-power FUS in the phantom model was investigated within a 3T MRI scanner for both employed sequences.

Synergistic Fe/Co Doping and Vacancy Engineering for Stable RuO in Acidic OER.

RuO is one of the most effective catalysts for the oxygen evolution reaction (OER) in proton-exchange membrane water electrolyzers (PEMWEs); however, its long-term stability is hindered by Ru dissolution under harsh electrochemical conditions. In this study, we explore the combined effects of metal doping and oxygen vacancy engineering on enhancing the stability performance of RuO-based catalysts. Using Fe or Co as dopants, we demonstrate that doping not only decreases the Ru valence but also induces a significant enrichment of oxygen vacancies.

BISMiS Live: a digital platform for global communication among microbial systematists.

BISMiS Live, launched on March 20, 2021, emerged as a timely response to the disruption caused by the CoViD-19 pandemic, offering a global virtual platform for discourse in microbial systematics. Spearheaded by the Bergey's International Society for Microbial Systematics (BISMiS), this monthly webinar series hosted renowned experts and engaged thousands of participants across 84 countries. The initiative emphasized inclusivity, scientific depth, and digital accessibility, with sessions archived on YouTube (@BISMiS_) for broader impact.

Potential ecological risk and accumulation of heavy metal(loid)s in soils of Macao in China as a non-industrial and tourist City.

With rapid industrialization, the accumulation and contamination of heavy metal(loid)s in soil often has occurred. However, the accumulation of heavy metal(loid)s in soil in non-industrial and tourist cities with high population densities is not well known. Therefore, the Macao Special Administrative Region (Macao SAR) was taken as an example to investigate the spatial and temporal changes in the characteristics of soil heavy metal(loid)s in non-industrial and tourist cities.

Dual-functionality of Nocardiopsis alba B57 in biocontrol and plant growth: a metabolomic approach to agricultural sustainability.

The increasing incidence of fungal phytopathogens poses a significant challenge to agricultural sustainability, necessitating the development of environmental alternatives to synthetic fungicides and mitigating their ecological impact. This study explores the efficiency of Nocardiopsis alba B57 to produce secondary metabolites with antifungal and plant growth-promoting properties. Untargeted metabolomics using ultra-high-performance liquid chromatography (UPLC-MS/MS) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses identified key metabolites (e.

Three new polyketones from mangrove endophytic fungus aspergillus sp. HMGh1-1 and their insecticidal activity.

A new phenylacetic acid, methoxy-graphislactones C (1), a new anthraquinone (1'E)-6,8-di-O-methyl averanti (2), a new phenanthrene derivative, 12-methyl entonaemin A (3) and five previously reported compounds (4-8) were isolated from the fermented extracts of the mangrove endophytic fungus Aspergillus sp. HMGh1-1. The structures and absolute configurations of the new compounds were determined by spectroscopic techniques (high-resolution mass spectrometry, NMR), ECD calculations, and single-crystal X-ray analysis.

Magnetic Resonance Imaging monitoring of histotripsy effects in agar phantom.

Background: While the technical aspects of histotripsy have seen significant advancements, research on Magnetic Resonance Imaging (MRI) monitoring remains limited.

Purpose: This preliminary study explored the use of conventional T2-Weighted (T2-W) Turbo Spin Echo (TSE) imaging to monitor histotripsy lesions in a pure agar gel, as a potential research tool for MRI-guided histotripsy (MRgHt).

Methods: Histotripsy experiments were conducted in a 3T MRI scanner using a 2% weight per volume agar solution in water.

DNA methylation in chronic kidney disease.

Chronic kidney disease (CKD) is a condition that affects people on a global scale. After various stages of progression, CKD is finally characterized by renal fibrosis. As the most pervasive and well-studied epigenetic modification, DNA methylation has recently been associated with the development of renal fibrosis.

Organocatalytic Enantioselective Construction of Chiral Tetraarylmethanes from 4-(2-Hydroxyphenyl)benzyl Alcohols.

Organocatalytic enantioselective construction of chiral tetraarylmethanes has been achieved via the formal substitution of 4-(2-hydroxyphenyl)benzyl alcohols. With the aid of chiral phosphoric acid, 2,4'-biphenyl quinone methides were formed in situ from α-functionalized alcohols, followed by stereoselective 1,8-addition of 2-arylpyrroles to give the target tetraarylmethanes in 72-96% yields with 75-95% ee. Density functional theory calculations elucidate the reaction mechanism, both revealing the origin of enantioselectivity and identifying the rate-determining step.

A rapid and efficient red-light-activated Cre recombinase system for genome engineering in mammalian cells and transgenic mice.

The Cre-loxP recombination system enables precise genome engineering; however, existing photoactivatable Cre tools suffer from several limitations, including low DNA recombination efficiency, background activation, slow activation kinetics, and poor tissue penetration. Here, we present REDMAPCre, a red-light-controlled split-Cre system based on the ΔPhyA/FHY1 interaction. REDMAPCre enables rapid activation (1-s illumination) and achieves an 85-fold increase in reporter expression over background levels.

Human exposure to organophosphate esters (OPEs): A meta-analytic review of levels and distribution.

With the global increase in organophosphate ester (OPE) usage, contamination has extended beyond environmental media into the human body, raising significant biosafety concerns. In this study, we conducted a meta-analysis of 254,581 data points from 98 studies spanning multiple regions worldwide. This large-scale synthesis allowed us to minimize regional bias and generate a quantitative profile of human OPE contamination and related risks.

Spatial distribution of enlarged perivascular spaces as a potential biomarker for distinguishing vascular dementia from Alzheimer's disease in older adults.

Objective: We aimed to examine the potential value of enlarged perivascular space (EPVS) volume as a biomarker for distinguishing between Alzheimer's disease (AD) and vascular dementia (VD) in older people.

Methods: A total of 93 patients with Alzheimer's disease and 122 patients with vascular dementia, all aged > 65 years and diagnosed via clinical and neuropsychological assessments, were enrolled in this study. A deep learning model based on VB-Net was utilized to automatically quantify the EPVS volume in four brain regions: the basal nuclei (BN), centrum semiovale (CSO), temporal lobe (T), and midbrain (MB).

sp. nov., sp. nov. and sp. nov., three novel species of the genus from different saline lakes.

Three Gram-stain-negative, non-motile and halotolerant bacterial strains, designated as SYSU LYC5161, SYSU LYC5162 and EGI FJ00037, were isolated from sediment samples of different saline lakes in China. The taxonomic positions of these isolates were determined using the polyphasic taxonomic analysis. Phylogenetic analyses based on 16S rRNA gene sequences and genome comparisons revealed that these strains formed distinct clades within related type species as N53, GCL8 and DSM 29507 from the genus .

Quercetin Alleviates Folic Acid-Induced Renal Fibrosis by Inhibiting Tubular Epithelial Cell Ferroptosis via EGFR/ACSL4 Pathway.

Ferroptosis has emerged as a critical contributor to the pathogenesis of chronic kidney disease (CKD). Quercetin, a promising therapeutic agent and flavonoid with potential antiferroptotic properties, has demonstrated renoprotective effects. However, its molecular mechanisms remain unclear.

A bioequivalent cornea cross-linking method using photo-initiators LAP and visible light.

Although cell-encapsulating hydrogels are of tremendous interest in regenerative medicine, few of them have been used in clinics and rarely used natural extracellular matrices as polymer precursors. One successful example is to use riboflavin (RF)/ultraviolet A (UVA) to cross-link corneal collagen, which has been used in clinics to halt disease progression in patients with corneal ectatic diseases. However, high-energy UVA and its action on RF cause tissue damage, particularly irreversible endothelium loss, and thus standard RF/UVA protocol may have limitations in treating patients at the advanced stage with thin cornea.