Acupuncture for Rapid Pain Relief and Restoration of Motor Function in Acute Lateral Ankle Sprains: A Randomized Controlled Trial Protocol Based on Infrared Thermography.

Purpose: Acute lateral ankle sprain (ALAS) is a prevalent clinical sports injury disease, rapid relief of symptoms and return to normal functional activity are urgent needs of ALAS patients. The aim of this study is to observe the immediate therapeutic effect of acupuncture on ALAS patients in the short term and to evaluate the efficacy at different time points through a multidimensional assessment system, which is expected to provide a simple, rapid, safe and effective therapeutic option for ALAS patients.

Patients And Methods: This is a single-center, prospective, randomized clinical trial.

Antiviral RNA interference in plants: increasing complexity and intertwining with other biological processes.

RNA interference (RNAi, also known as RNA silencing) is one of the most important plant defense responses to combat viral invasions. Although the major components of the RNAi pathway, the steps leading to viral small interference RNA (siRNA) biogenesis, and the strategies of viral counter-defense via RNAi suppressors have been well studied, the broader roles of RNAi in virus infection and seed transmission remain less thoroughly reviewed. In particular, the increasing complexity of RNAi-associated mechanisms and their integration with other biological processes have not been comprehensively summarized.

Lattice O-O ligands in Fe-incorporated hydroxides enhance water oxidation electrocatalysis.

Understanding the structural dynamics of ligands and their interaction with catalytic centres under reaction conditions remains a fundamental challenge, yet it is essential for catalyst design. Here we reveal an in situ transformation of Ni-Fe hydroxide into a stable superoxo-hydroxide phase, which is accompanied by the formation of lattice O-O (O-O) ligands, as demonstrated using operando O-labelling spectroelectrochemistry and machine-learning-assisted global optimization. By correlating the intrinsic activity of Fe with the O-O concentration across a series of Fe-incorporated transition-metal hydroxides and oxides, we demonstrate that O-O triggers Fe activation for oxygen evolution electrocatalysis-a finding further supported by first-principles calculations.

Aberrant brain network connectivity related to cognitive and emotional regulation in women with abdominal obesity.

Abdominal obesity (AO) exhibits unique endocrine and metabolic characteristics in women, yet its neural mechanisms remain unclear. The aim of this study was to investigate the neural mechanisms of AO in females by examining functional connectivity (FC) alterations across brain networks. Resting-state functional MRI (rs-fMRI) data were collected from 38 female AO patients and 24 healthy controls (HCs).

Association between hepatic steatosis index and impaired fasting glucose: a multicenter retrospective cohort study in China.

Background: The Hepatic Steatosis Index (HSI) is a simple screening tool for adults with non-alcoholic fatty liver disease (NAFLD). While lipid and glucose metabolism are closely interrelated, few studies have examined the association between HSI and impaired fasting glucose (IFG). This study aims to investigate the relationship between HSI and IFG risk in a large Chinese cohort.

Role of the beneficial phyllosphere microbiome in the defense against red clover anthracnose caused by Colletotrichum americae-borealis.

Red clover (Trifolium pratense), a high-quality forage plant, faces significant threats from anthracnose in northeastern China, but the pathogen responsible remains unidentified. The phyllosphere microbiota is crucial in plantpathogen interactions, yet its role in the resistance of red clover to anthracnose is poorly understood. Using morphological, molecular, and multigene phylogenetic analyses, we identified Colletotrichum americae-borealis (Cab) as the pathogen that causes anthracnose in red clover in China.

Cucumber green mottle mosaic virus encodes additional small proteins with specific subcellular localizations and virulence function.

The vast majority of known viruses belong to the positive-sense single-stranded RNA (+ssRNA) class. Tobamoviruses are among the most destructive plant viruses and threaten global food security. It is generally accepted that +ssRNA viruses including tobamoviruses encode proteins solely on their positive strand (+RNA).

Genomic characterisation and traceability analysis of a Clostridium botulinum strain involved in a food poisoning incident.

Objective: To identify the source of Clostridium botulinum (C. botulinum) involved in a food poisoning case in Kunming and analyze its molecular characteristics.

Results: This study examined samples from a clustered food poisoning incident, including pickled pig liver consumed by three patients, hospital vomit from a deceased patient, and household waste mixed with vomit.

The Isolation of Lead-Tolerant PGPR from Red Clover Soil and Its Role in Promoting the Growth of Alfalfa.

Alfalfa ( L.) is an outstanding species used for the remediation of heavy metal-contaminated soil, and our previous research has shown that PGPR can promote plant growth under high-concentration lead stress. This discovery has forced scientists to search for PGPR strains compatible with alfalfa to develop an innovative bioremediation strategy for the remediation of lead-contaminated soil.

SLMO transfers phosphatidylserine between the outer and inner mitochondrial membrane in Drosophila.

Phospholipids are critical building blocks of mitochondria, and proper mitochondrial function and architecture rely on phospholipids that are primarily transported from the endoplasmic reticulum (ER). Here, we show that mitochondrial form and function rely on synthesis of phosphatidylserine (PS) in the ER through phosphatidylserine synthase (PSS), trafficking of PS from ER to mitochondria (and within mitochondria), and the conversion of PS to phosphatidylethanolamine (PE) by phosphatidylserine decarboxylase (PISD) in the inner mitochondrial membrane (IMM). Using a forward genetic screen in Drosophila, we found that Slowmo (SLMO) specifically transfers PS from the outer mitochondrial membrane (OMM) to the IMM within the inner boundary membrane (IBM) domain.

Effect of Acupoint Catgut Embedding on Subjective Appetite in Overweight and Obese Adults with Strong and Moderate Appetite: A Secondary Analysis of a Randomized Clinical Trial.

Background: Appetite plays a crucial role in obesity and weight loss outcomes. while conventional therapies reduce appetite, They often have limitations. Acupoint Catgut Embedding (ACE) is widely used for weight loss, but its impact on subjective appetite, especially across different appetite status, remains underexplored.

A fast 2D MoS photodetector with ultralow contact resistance.

Two-dimensional (2D) transition metal dichalcogenides (TMDs), such as molybdenum disulfide (MoS), hold great promise for next-generation nanoelectronic and nanophotonic devices. While high photoresponsivity and broad spectral coverage (UV-IR) have been reported, the slow response time of MoS photodetectors caused by their unfavorable RC characteristics is still a major limit in current devices. Once the RC limit issue is resolved, the intrinsic saturation drift velocity of electrons in TMDs (∼10 cm s) may enable GHz opto-electronic operations.

Electrically tunable non-radiative lifetime in WS/WSe heterostructures.

Van der Waals heterostructures based on transition metal dichalcogenides (TMDs) have emerged as excellent candidates for next-generation optoelectronics and valleytronics, due to their fascinating physical properties. The understanding and active control of the relaxation dynamics of heterostructures play a crucial role in device design and optimization. Here, we investigate the back-gate modulation of exciton dynamics in a WS/WSe heterostructure by combining time-resolved photoluminescence (TRPL) and transient absorption spectroscopy (TAS) at cryogenic temperatures.

Immediate Efficacy of Contralateral Acupuncture on SI3 Combined with Active Exercise for Acute Lumbar Sprains: Protocol for a Randomized Controlled Trial.

Purpose: Acute lumbar sprain (ALS) is a common clinical disease characterized by persistent intolerable low back pain and limitation of movement, and quick pain relief and restoration of mobility in a short time are the main needs of patients when they visit the clinic. This study aims to evaluate the immediate efficacy of contralateral acupuncture (CAT) on SI3 combined with active exercise in treating ALS.

Methods And Analysis: This study is a randomized controlled trial which will recruit 118 eligible participants aged 18 to 55 years with ALS at the Second Affiliated Hospital of Yunnan University of Chinese Medicine between March 2024 and December 2026.

Genome-wide identification and characterization of NAC transcription factor family members in Trifolium pratense and expression analysis under lead stress.

Background: The NAC TF family is widely involved in plant responses to various types of stress. Red clover (Trifolium pratense) is a high-quality legume, and the study of NAC genes in red clover has not been comprehensive. The aim of this study was to analyze the NAC gene family of red clover at the whole-genome level and explore its potential role in the Pb stress response.

Identification and Evolutionary Analysis of the Auxin Response Factor (ARF) Family Based on Transcriptome Data from Caucasian Clover and Analysis of Expression Responses to Hormones.

Caucasian clover ( M. Bieb.) is an excellent perennial plant in the legume family Fabaceae, with a well-developed rhizome and strong clonal growth.

Physiological characteristics, rhizosphere soil properties, and root-related microbial communities of Trifolium repens L. in response to Pb toxicity.

Trifolium repens L. (T. repens) is considered a potential phytoremediation species due to its large biomass and ability to accumulate and tolerate heavy metals.

Effect of regional anesthesia and analgesia on long-term survival following abdominal cancer Surgery-A systematic review with meta-analysis.

Background: The impact of regional anesthesia and analgesia (RAA) on long-term survival following cancer surgery is a topic of debate. The aim of this study was to investigate the effects of perioperative RAA on long-term oncological outcomes in patients undergoing major abdominal cancer surgery.

Methods: The authors searched computerized databases and reference lists from inception to December 20, 2022.

mA modification of plant virus enables host recognition by NMD factors in plants.

N-methyladenosine (mA) is the most abundant eukaryotic mRNA modification and is involved in various biological processes. Increasing evidence has implicated that mA modification is an important anti-viral defense mechanism in mammals and plants, but it is largely unknown how mA regulates viral infection in plants. Here we report the dynamic changes and functional anatomy of mA in Nicotiana benthamiana and Solanum lycopersicum during Pepino mosaic virus (PepMV) infection.

Immediate analgesic effect of acupuncture intervention within 10 min during acute migraine attacks: protocol of a randomised controlled trial.

Introduction: Migraine is a widespread neurological disorder characterised by recurrent moderate-to-severe headaches. These headaches can seriously affect patients' daily life and work, especially during acute attacks when patients often need immediate pain relief. This study aims to assess the immediate analgesic effect of acupuncture for 10 min during acute migraine attacks.

Effect of catgut embedding at acupoints versus non-acupoints in abdominal obesity: a randomized clinical trial.

Objective: To explore the difference of catgut embedding effect between acupoints and non-acupoints in patients with abdominal obesity (AO).

Methods: In this multicenter, double-blind, randomized controlled trial, all subjects were randomly assigned into the acupoint catgut embedding (ACE) group and control group (catgut embedding at non-acupoints). With a 12-week actual intervention period and a 4-week period of follow-up.

Functional identification of a novel C7 protein of tomato yellow leaf curl virus.

Tomato yellow leaf curl virus (TYLCV) is a monopartite geminivirus, and one of the most devastating plant viruses in the world. TYLCV is traditionally known to encode six viral proteins in bidirectional and partially overlapping open reading frames (ORFs). However, recent studies have shown that TYLCV encodes additional small proteins with specific subcellular localizations and potential virulence functions.