Exploring omics strategies for drug discovery from isolated from the marine ecosystem.

Marine are prolific producers of diverse bioactive secondary metabolites, making them vital for drug discovery. Traditional cultivation and bioassay-guided isolation techniques often lead to the rediscovery of the same compounds, revealing the limitations of these traditional approaches and emphasizing the need for more advanced methods. The emergence of omics technologies such as genomics, metagenomics, transcriptomics, and metabolomics has dramatically enhanced the ability to investigate microorganisms by providing detailed insights into their biosynthetic gene clusters, metabolic pathways, and regulatory mechanisms.

Mobilome-mediated transcriptional activation of biosynthetic gene clusters and its impact on strain competitiveness in food fermentation microbiomes.

Background: Microbial interactions are critical for maintaining the stability of food fermentation microbiomes, and mobile genetic elements (MGEs) significantly influence these interactions by horizontal gene transfer events. Although MGEs are known to facilitate horizontal gene transfer, their distribution among microorganisms and specific effects on microbial interactions remain poorly understood.

Results: We analyzed 590 metagenomic and 42 metatranscriptomic samples from food fermentations, recovering 1133 metagenome-assembled genomes (MAGs).

Altererythrobacter guangdongensis sp. nov. and Tsuneonella sediminis sp. nov., two novel carotenoid-producing species isolated from tidal flat sediment.

Four aerobic, Gram-stain-negative, rod-shaped, and non-motile bacterial strains, designated SYSU HZ0097ᵀ, SYSU HZ0136, SYSU LHT278ᵀ, and SYSU LHT272, were isolated from tidal flat sediment collected in Zhuhai, China. Phylogenetic analysis based on 16S rRNA gene sequences assigned all four strains to the family Erythrobacteraceae. Strains SYSU HZ0097ᵀ and SYSU HZ0136 showed the highest 16S rRNA gene sequence similarity (98.

BISMiS Live: a digital platform for global communication among microbial systematists.

BISMiS Live, launched on March 20, 2021, emerged as a timely response to the disruption caused by the CoViD-19 pandemic, offering a global virtual platform for discourse in microbial systematics. Spearheaded by the Bergey's International Society for Microbial Systematics (BISMiS), this monthly webinar series hosted renowned experts and engaged thousands of participants across 84 countries. The initiative emphasized inclusivity, scientific depth, and digital accessibility, with sessions archived on YouTube (@BISMiS_) for broader impact.

Dual-functionality of Nocardiopsis alba B57 in biocontrol and plant growth: a metabolomic approach to agricultural sustainability.

The increasing incidence of fungal phytopathogens poses a significant challenge to agricultural sustainability, necessitating the development of environmental alternatives to synthetic fungicides and mitigating their ecological impact. This study explores the efficiency of Nocardiopsis alba B57 to produce secondary metabolites with antifungal and plant growth-promoting properties. Untargeted metabolomics using ultra-high-performance liquid chromatography (UPLC-MS/MS) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses identified key metabolites (e.

sp. nov., sp. nov. and sp. nov., three novel species of the genus from different saline lakes.

Three Gram-stain-negative, non-motile and halotolerant bacterial strains, designated as SYSU LYC5161, SYSU LYC5162 and EGI FJ00037, were isolated from sediment samples of different saline lakes in China. The taxonomic positions of these isolates were determined using the polyphasic taxonomic analysis. Phylogenetic analyses based on 16S rRNA gene sequences and genome comparisons revealed that these strains formed distinct clades within related type species as N53, GCL8 and DSM 29507 from the genus .

Quercetin Alleviates Folic Acid-Induced Renal Fibrosis by Inhibiting Tubular Epithelial Cell Ferroptosis via EGFR/ACSL4 Pathway.

Ferroptosis has emerged as a critical contributor to the pathogenesis of chronic kidney disease (CKD). Quercetin, a promising therapeutic agent and flavonoid with potential antiferroptotic properties, has demonstrated renoprotective effects. However, its molecular mechanisms remain unclear.

A new white-flowered species of (Liliaceae) from the Fergana Valley, Uzbekistan and Kyrgyzstan.

The article describes the morphological and anatomical features of Levichev, Turginov & W.J.Li, , recently described from the Fergana Valley.

Nitrogen fixation in Pampinifervens, a new species-rich genus of Aquificaceae that inhabits a wide pH range in terrestrial hot springs.

Members of the Aquificota play important roles in hydrogen, sulfur, and carbon cycles in geothermal systems as ubiquitous and abundant chemolithoautotrophs; however, their roles in the nitrogen cycle are poorly defined. Here, we show that Aquificaceae isolate T-2 from Tengchong, China, fixes nitrogen based on growth with dinitrogen as the sole nitrogen source and incorporation of N atoms when grown with N. We further show evidence that suggests its nitrogen fixation (nif) genes are transcribed in situ in a nearby spring.

Genomic and taxonomic characterization of Niallia pakistanensis sp. nov. NCCP-28: a novel antibiotic-resistant and heavy-metal-tolerant bacterium isolated from the legume rhizosphere in Pakistan.

Soil microbes play a crucial role in biogeochemical cycling and are a valuable resource for discovering novel taxa with environmental applications. This study aimed to explore rhizospheric microbial diversity for potential biotechnological and ecological relevance. The taxonomic placement of strain NCCP-28 was determined through polyphasic characterization.

Polyphasic taxonomy and genome mining of gen. nov., sp. nov., a novel psychrotolerant pectin-degrading actinobacterium isolated from desert soil.

Extreme arid environments harbor diverse yet underexplored microorganisms with unique ecological adaptations and significant biotechnological potential. Here, we characterized a novel actinobacterium, strain SYSU D00978, isolated from desert soil in the Gurbantunggut Desert, Xinjiang, China. The strain was Gram-stain-positive, aerobic, curved-rod-shaped, non-motile, catalase-positive, and oxidase-negative.

sp. nov., a novel bacterium of the genus isolated from sediment in Yuncheng Salt Lake.

Three Gram-stain-negative, aerobic, rod-shaped, yellow-pigmented bacteria, designated YC-516-91, YC-516-48 and YC-516-93, were isolated from a sediment sample of Yuncheng Salt Lake in China. Growth occurred at 15-42 ℃ (optimum 32-37 ℃), pH 6.0-10.

sp. nov., sp. nov. and sp. nov., three novel chitin-degrading actinobacteria isolated from a mudflat sediment.

Three strains of actinobacteria, designated as SYSU T00194, SYSU T00242 and SYSU T00266, were isolated from tidal flat sediment samples in Zhanjiang, PR China. The taxonomic position of three isolates was determined using polyphasic taxonomic analysis and phylogenomic analysis. Phylogenetic analysis and 16S rRNA gene sequence comparisons indicated that strain SYSU T00194 formed a distinct clade with IFO 16236 and shared a sequence identity value of 97.

Characterization of a thermostable uricase derived from YIM 77501 and its heat-resistant mechanism.

Introduction: Uricases are oxidative enzymes that catalyze the conversion of uric acid to allantoin and hydrogen peroxide, widely utilized in uric acid testing and the treatment of gout, hyperuricemia, and Tumor Lysis Syndrome (TLS). The search for uricases with long-term thermostability has become a significant area of research.

Methods: In this study, a uricase gene (truox) was obtained from the genome of YIM 77501, which was subsequently cloned and heterologously expressed.

Rare and abundant taxa in Artemisia desertorum rhizosphere soils demonstrate disparate responses to drought stress.

The growth and adaptability of desert plants depend on their rhizosphere microbes, which consist of a few abundant taxa and numerically dominant rare taxa. However, the differences in diversity, community structure, and functions of abundant and rare taxa in the rhizosphere microbiome of the same plant in different environments remain unclear. This study focuses on the rhizosphere microbial communities of Artemisia desertorum, a quintessential desert sand-stabilizing plant, investigating the diversity patterns and assembly processes of rare and abundant taxa across four Chinese deserts: Mu Us, Kubuqi, Tengger, and Ulan Buh.

Flavobacterium aestuarii sp. nov., a novel species isolated from the Pearl River Estuary.

Strain SYSU M80003, a Gram-stain-negative and aerobic bacterium, was isolated from water sample of the Pearl River Estuary, Guangzhou, Guangdong, PR China. The strain could grow at 4-37 °C (optimal: 28 °C), pH 6.0-8.

Combined toxicity of polyethylene microplastics and soil salinization to earthworms is generally antagonistic or additive.

Microplastic (MP) accumulation and salinization frequently co-occur in terrestrial ecosystems, posing potential risks to soil health. However, their combined toxicity to soil invertebrates remains unclear. This study investigated combined effects of NaCl and polyethylene MPs (< 35, < 125, < 500 μm) on earthworms.

Characterization of Sphingomicrobium aquimarinum sp. nov. and Sphingomicrobium maritimum sp. nov. highlights astaxanthin-producing bacteria in the family Sphingomonadaceae.

Bacteria are considered as better models for industrial production of astaxanthin (AXT) because of their fast growth and convenience for the downstream extraction. Nevertheless, few bacterial species of native AXT producers are explored to date. In this study, bacterial strains XHP0235 (=GDMCC 1.

GPR40 activation alleviates pulmonary fibrosis by repressing M2 macrophage polarization through the PKD1/CD36/TGF-β1 pathway.

Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive interstitial lung disease characterized by complex aetiologies involving the accumulation of inflammatory cells, such as macrophages, in the alveoli. This process is driven by uncontrolled extracellular matrix (ECM) deposition and the development of fibrous connective tissues. Here, we observed that the mRNA expression of Ffar1, the gene encoding G protein-coupled receptor 40 (GPR40), is repressed, while Cd36 is increased in the bronchoalveolar lavage fluid (BALF), which is predominantly composed of alveolar macrophages, of IPF patients.

Current techniques for the treatment of spasticity and their effectiveness.

This review highlights the role of existing spasticity treatment methods in reducing muscle tone and improving function. The surgical methods today mainly include selective dorsal rhizotomy, selective neurotomy, intrathecal baclofen treatment (ITB), etc. These techniques (except ITB) can lower patients' muscular tone in the long term and improve function to some extent.

gen. nov., sp. nov., isolated from the Gurbantunggut Desert soil.

A Gram-stain-negative, aerobic, rod-shaped bacterial strain, SYSU D60014, was isolated from a sandy soil sample collected from the Gurbantunggut Desert in Xinjiang, PR China. Colonies of SYSU D60014 were pink-coloured, crystalline, irregular edge with a rough surface. Phylogenetic analyses based on both the 16S rRNA gene and whole-genome sequences assigned strain SYSU D60014 belonged to the family , formed a distinct lineage and showed 92.

Characterization, thermostable mechanism, and molecular docking of a novel glucose-tolerant -glucosidase/-galactosidase from the GH1 family isolated from Rehai hot spring.

Introduction: As a renewable alternative to fossil fuels, second-generation bioethanol production relies heavily on efficient lignocellulose conversion, with -glucosidase playing a critical role.

Methods: This study focused on the -glucosidase gene discovered in the Tengchong Rehai metagenome. The recombinant enzyme Y50Bg4 was obtained through PCR amplification, cloning, and expression.