Green synthesis of -based silver nanoparticles: characterization and transdermal delivery for anti-inflammatory applications.

The current study aims to develop chitosan transdermal patches containing AgNPs synthesized and characterized from to reduce inflammation. Silver nanoparticles (AgNPs) were prepared utilizing the extract and characterized through Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), zeta potential measurements, and particle size analysis. The AgNPs were incorporated into chitosan-based transdermal patches, which were assessed for physicochemical properties, dermal compatibility, and anti-inflammatory effectiveness.

Defect-engineered amorphous-like nanointerceptors for T MRI-Guided treatment of reperfusion injury.

Ischemic reperfusion (I/R) injury is dominated by excessive reactive oxygen species (ROS)-mediated oxidative damage and uncontrolled inflammation, yet effective strategies for simultaneous diagnosis and treatment remain elusive. Herein, we report a defect-engineered amorphous-like MnCeO nanointerceptor with dual capabilities of magnetic resonance imaging (MRI) -guided stroke diagnosis and ROS-scavenging therapy. The synergistic effect of the amorphous-like structure and Mn-Ce solid solution induces abundant oxygen vacancies and a disordered surface, significantly boosting ROS catalytic removal.

aEEG as an early predictor of brain injury and prognosis in neonates with hypoxic-ischemic encephalopathy.

Objective: This study aims to evaluate the independent risk factors associated with neonatal hypoxic-ischemic encephalopathy (HIE) using amplitude-integrated electroencephalography (aEEG), specifically exploring the relationship between aEEG scores, clinical manifestations, neurodevelopmental assessments, and neuron-specific enolase (NSE) levels with adverse outcomes in HIE.

Methods: A retrospective analysis was performed on clinical data from 224 neonates diagnosed with HIE who were admitted between January 2022 and May 2024. Infants were grouped by HIE severity: mild, moderate, and severe.

Hepcidin/HIF-1α is involved in the endogenous recovery and ferroptosis after cerebral ischemia in mice.

It is common to witness a spontaneous endogenous recovery process with the potential mechanisms involving the remyelination, nerve regeneration, and synaptic plasticity, which might be vital for the neurological rehabilitation after cerebral ischemic stroke. Iron homeostasis is considered to be crucial for myelination and neuronal metabolic processes, and Hepcidin is an important factor in regulating iron homeostasis and ferroptosis. Hypoxia-inducible factor (HIF) is an important hypoxia tolerance factor and can regulate cell survival in hypoxia environment, which is critical to regulate iron homeostasis as well.

The Obesity-Related Indices Are Useful for Predicting Diabetic Nephropathy in Patients With Type II Diabetes Mellitus: A Retrospective Cohort Study of NHANES.

Background And Aim: This study investigated the correlation between obesity-related indices and diabetic nephropathy (DN) in patients with type II diabetes mellitus (T2DM). It aimed to provide a new predictive assessment tool for the clinic and a scientific basis for managing DN.

Methods: The data utilised in this study were obtained from the National Health and Nutrition Examination Survey (NHANES) database, spanning 2007 to 2018.

HADHA-mediated regulation of JAK/STAT3 signaling in glioblastoma: a metabolic-epigenetic axis.

Glioblastoma multiforme (GBM) is one of the most aggressive forms of brain cancer, characterized by rapid growth and resistance to conventional therapies. This study investigates the role of HADHA, a key enzyme in fatty acid β-oxidation, in the progression of GBM. we show that the overexpression of HADHA in GBM correlates with a poor prognosis in patients and plays a role in promoting tumor growth and invasion.

Relationship between first pass effect NLR and PLR in mechanical thrombectomy for acute anterior circulation large-vessel occlusion.

Purpose: The study investigated the correlation between the neutrophil-lymphocyte ratio (NLR) and the platelet-lymphocyte ratio (PLR) concerning the first-pass effect (FPE) observed during mechanical thrombectomy subsequent to acute ischemic stroke (AIS).

Methods: Patients diagnosed with AIS in the anterior circulation, who underwent mechanical thrombectomy between January 2020 and December 2022, were assessed. Various data were collected, including blood cell counts, general information, relevant surgical and clinical details, and functional outcomes determined by the Modified Rankin Scale (MRS) score ≤2 at 3 months.

Identification of differentially co-expressed genes with lipid metabolism in Parkinson's disease by bioinformatics analysis.

There was increasing evidence that lipid metabolism disorders played a significant part in the maturation of Parkinson's disease (PD). The purpose of the article was to investigate a significance of lipid metabolism-related genes (LMRGs) in the maturation of PD. The function of LMRGs in the etiology of PD was explored by analysing PD datasets from Gene Expression Omnibus.

Evaluation of the Correlation between Gut Microbiota and Renal Function in Chronic Kidney Disease Patients.

Chronic kidney disease (CKD) has recently emerged as a significant, global public health threat, and the gut microbiota are now recognized as playing a crucial role in the pathogenesis and progression of CKD. In this study, we investigated the correlation between differential gut microbiota and renal function impairment by analyzing the structure, diversity, and other characteristics of the gut microbiota in patients with CKD. Our findings indicated that CKD patients exhibit decreased species diversity and evenness in their gut microbiota compared to healthy individuals, with notable differences in community composition between the groups.

Efficacy of rTMS on SCZ symptoms: Insights from connectome mapping.

Repetitive transcranial magnetic stimulation (rTMS) is a potential treatment for schizophrenia (SCZ), yet its efficacy and underlying mechanisms remain uncertain. This study evaluates the impact of rTMS targeting the right orbitofrontal cortex (OFC) in SCZ patients and constructs a connectome atlas based on symptomatology and treatment responses. Ninety-eight SCZ patients were randomly assigned to rTMS (n = 53) or sham stimulation (n = 45) groups.

Impact of digital health management on clinical outcomes during post-PCI outpatient care in patients with acute coronary syndrome: study protocol for a multicentre, randomized controlled trial.

Introduction: Coronary heart disease is becoming more common in developing nations. Acute coronary syndrome (ACS) poses a major challenge to global health; however, access to cardiac rehabilitation and preventative measures is limited. There is an urgent need for affordable and widely accessible alternative delivery models to resolve this treatment gap.

Carbon dots derived from Ligusticum Chuanxiong mitigate cardiac injury by disrupting the harmful oxidative stress-apoptosis cycle.

Background: Myocardial ischemia-reperfusion injury (MIRI) represents a significant complication following myocardial infarction surgery, for which preventive strategies remain limited. The primary pathological characteristics of MIRI include oxidative stress and apoptosis.

Results: This study presents the synthesis of carbon dots derived from Ligusticum Chuanxiong (LC-CDs) through the application of the hydrothermal method.

Auraptene Mitigates Cigarette Smoke and Lipopolysaccharide-Induced Chronic Obstructive Pulmonary Disease in Mice and BEAS-2B Cells via Regulating Keap1/Nrf2/HO-1 Pathway.

Chronic obstructive pulmonary disease (COPD) is a most common respiratory condition characterized by airflow limitation, airway inflammation, and lung injury. The present study was undertaken to unveil the therapeutic potentials of the auroptene against lipopolysaccharide (LPS) and cigarette smoke (CS)-induced COPD in mice. The CS along with LPS was exposed to healthy C57BL/6 mice through the intranasal route to induce COPD.

Standardized operating room nursing care is effective in preventing the occurrence of surgical wound infections.

Objective: To analyze the clinical effectiveness of standardized operating room nursing care in preventing wound infections from orthopedic surgery.

Methods: A total of 128 patients requiring orthopedic surgery were selected and divided into a control group and an observation group in accordance with the nursing care they received during surgery, with 64 patients in each group. Patients in the control group received conventional operating room nursing care, while those in the observation group received standardized operating room nursing care.

Effects of minocycline on patients with acute anterior circulation ischaemic stroke undergoing intravenous thrombectomy (MIST-A): the study protocol for a multicentre, prospective, randomised, open-label, blinded-endpoint trial.

Introduction: Despite the implementation of mechanical thrombectomy, acute ischaemic stroke with large vessel occlusion (AIS-LVO) remains a significant health concern, characterised by substantial morbidity and mortality. Our trial aims to evaluate the efficacy and safety of minocycline in reducing infarct volume and improving functional outcomes in patients undergoing mechanical thrombectomy for anterior circulation AIS-LVO.

Methods And Analysis: The MIST-A trial is a prospective, randomised, open-label, blinded-endpoint trial to be conducted across 12 medical centres.

Intra-Arterial Urokinase After Endovascular Reperfusion for Acute Ischemic Stroke: The POST-UK Randomized Clinical Trial.

Importance: Persisting or new thrombi in the distal arteries and the microcirculation have been reported to limit the benefits of successful endovascular thrombectomy for patients with acute ischemic stroke. It remains uncertain whether intra-arterial thrombolysis by urokinase following near-complete to complete reperfusion by thrombectomy improves outcomes among patients with ischemic stroke due to large vessel occlusion.

Objective: To assess the efficacy and adverse events of intra-arterial urokinase after near-complete to complete reperfusion by thrombectomy for acute ischemic stroke due to large vessel occlusion.

Cardioprotective effect of Perakine against myocardial ischemia-reperfusion injury of type-2 diabetic rat in Langendorff-Perfused Rat Hearts: Role of TLR4/NF-kB signalling pathway.

Myocardial ischemia-reperfusion (I/R) injury is a grave life-threatening situation, if not treated well in time. The development of natural remedies for myocardial I/R injury has witnessed dramatic growth in the last decade. Prompted by the above, in the present study, we have elucidated the pharmacological effect of Perakine (PER), an indole alkaloid in myocardial ischemia-reperfusion injury in type 2 diabetic rats.

Amorphophallus campanulatus tuber extract protects diabetic nephropathy in streptozotocin-induced diabetic nephropathy rat model by regulating oxidative stress and TNF-α inflammatory pathway.

Purpose: To assess the effect of Amorphophallus campanulatus tuber (Ac) extract in the protection of diabetic nephropathy in streptozotocin (STZ) induced diabetic nephropathy (DN) rat model.

Methods: Diabetes was induced with STZ (60 mg/kg, i.p.

Application of individual brain connectome in chronic ischemia: mapping symptoms before and after reperfusion.

How brain functions in the distorted ischemic state before and after reperfusion is unclear. It is also uncertain whether there are any indicators within ischemic brain that could predict surgical outcomes. To alleviate these issues, we applied individual brain connectome in chronic steno-occlusive vasculopathy (CSOV) to map both ischemic symptoms and their postbypass changes.

An Oxidative Cleavage-Based Cruciform DNA Nanostructure for Hypochlorous Acid Visualization to Monitor Intestinal Inflammation.

Ulcerative colitis is a persistent inflammatory bowel disease characterized by inflammation and ulceration in the colon and gastrointestinal tract. It was indicated that the generation of hypochlorous acid (HClO) through the enzymatic activity of myeloperoxidase is significantly linked to ulcerative colitis. In this study, by assembling two hairpins (Hpa and Hpb) onto a quadrivalent cruciform DNA nanostructure, a novel HClO-activatable fluorescent probe was developed based on DNA nanomaterials (denoted MHDNA), which is sensitive, economic, simple, and stable.

Eupatilin mitigates Gestational diabetes in streptozotocin-induced diabetic pregnant rats through the Regulation of inflammation and oxidative stress.

Gestational diabetes mellitus (GDM) is a common metabolic disease that is typically diagnosed in pregnant women. The current study was aimed at disclosing the salutary activities of eupatilin against streptozotocin (STZ)-induced GDM in rats. The pregnant rats were induced with GDM and then treated with eupatilin for 20 days.

Relationship between astrocyte damage and different levels of cerebrospinal fluid markers and prognosis in patients with subarachnoid hemorrhage.

Introduction: The aim of the study was to explore the relationship between astrocyte damage and different levels of cerebrospinal fluid markers and prognosis in patients with subarachnoid hemorrhage (SAH).

Material And Methods: A total of 168 SAH patients diagnosed and treated in the emergency department of our hospital during the period October 2019 to February 2022 were randomly selected as the study subjects. The severity of these patients' condition was evaluated by Hunt-Hess grading and these subjects were graded as the low-level group (78 patients) and high-level group (90 patients) according to the evaluation results.

The effects of propolis-loaded chitosan nanoparticles and menstrual blood stem cells on LPS-induced ovarian inflammation in the murine ovary in vivo: An in vitro and in vivo study.

Mammary glands infection via Gram-negative bacteria may cause infertility or reduced ovarian function. In the current study, a potential treatment for LPS-induced ovarian inflammation was developed. Propolis was loaded into chitosan nanoparticles and co-administered with menstrual blood stem cells (MenSCs) in mice infused with LPS.

Reducing myocardial infarction by combination of irisin and Lindl through inhibiting nod-like receptor protein-3-related pyroptosis and activating PINK1/Parkin-mitophagy during aging.

Aging, a crucial risk factor for ischemic heart disease, has negative impacts on cardioprotective mechanisms. As such, there is still an unmet requirement to explore potential therapies for improving the outcomes of myocardial ischemia/reperfusion (IR) injury in elderly subjects. Here, we aimed to confirm the cardioprotective function of irisin/Dendrobium nobile Lindl (DNL) combination therapy against myocardial IR injury in aged rats, with a focus on the involvement of pyroptosis and mitophagy.

Detection of copy number variations based on a local distance using next-generation sequencing data.

As one of the main types of structural variation in the human genome, copy number variation (CNV) plays an important role in the occurrence and development of human cancers. Next-generation sequencing (NGS) technology can provide base-level resolution, which provides favorable conditions for the accurate detection of CNVs. However, it is still a very challenging task to accurately detect CNVs from cancer samples with different purity and low sequencing coverage.