Pore-C Pipeline-Toolbox: a comprehensive pipeline for Pore-C data analysis.

Three-dimensional (3D) genomic architecture is crucial for the regulation of different biological processes, and the study of multi-way chromatin structures represents a cutting-edge area in this field. Pore-C is an advanced experimental technique integrating chromosome conformation capture (3C) with Nanopore long-read sequencing, which can effectively capture complex multi-way chromatin contacts across the genome. Due to the absence of comprehensive and dedicated tools, the analysis of Pore-C data remains challenging, restricting its broader application.

Recent progress of DNA walker and its applications in food safety analysis.

Food hazards cause severe human illness and death worldwide every year. DNA walker possesses ingenious design and adaptable function. The structural advantage enables the DNA walker to accurately identify molecules and perform efficient mechanical movement.

Linking Ecological and Biogeographical Traits to Body Length and Dietary Breadth in Snakes.

Body size and dietary breadth are important for understanding animal evolution and adaptation. Snakes, as ecologically diverse predators with wide variation in morphology, reproduction, and diet, provide an excellent species group to explore how venom, reproductive mode, and biogeographical traits shape the evolution of ecological traits and dietary breadth. In this study, we compiled a global dataset of 4190 snake species and applied phylogenetically informed models to examine how traits such as venom, litter size, reproductive mode, and biogeographical characteristics across their geographic ranges (temperature, precipitation, and NDVI) influence body size and dietary breadth.

Uncovering the CO emission evolution and reduction trends of industrial boilers in China.

Industrial boilers are an important anthropogenic source of CO emissions globally due to their considerable energy consumption. This study investigated the spatio-temporal heterogeneity and future reduction potential of CO emissions using the first established long time-series CO emission inventory of Chinese industrial boilers based on the updated localised emission factors and sub-fuel type activity level databases. Results showed that CO emissions from Chinese industrial boilers were estimated at 786.

Progress in advanced biosensors for authentication of animal-origin food.

Economically motivated adulteration (EMA) has become a critical issue in authentication of animal-origin food, as it poses serious threat to consumer benefits and health, industry development, and religious concerns. Efficient detection technologies are urgently needed to ensure food authenticity. However, conventional authentication technologies are limited in their capabilities for portable and highly sensitive detection.

Recurrent Spontaneous Cerebrospinal Fluid Rhinorrhea With Meningitis due to a Clival Defect and Literature Review.

Most patients with spontaneous cerebrospinal fluid (CSF) rhinorrhea have fistulas located at the cribriform plate or sphenoid sinus, with very few cases at the clivus. Here, we report a patient with recurrent spontaneous cerebrospinal fluid rhinorrhea complicated by meningitis due to a clival defect. Under different body positionings, computed tomography scans were performed to localize the fistula, which was repaired successfully with the autologous fascia and adipose tissue through an endoscopic endonasal approach.

Impact of Controlled Magneto-Structural Coupling in MnCoGe-Based Compounds on the Design of Multifunctional Materials for Technological Advances.

Tailoring the magneto-structural coupling in magnetic martensitic materials is pivotal for optimizing multifunctional properties such as magnetocaloric effect (MCE) and negative thermal expansion (NTE). This study demonstrates how Ni substitution in Mn NiCoGe (x = 0.03 to 0.

Impact of organic carbon concentration on the molecular properties and assembly of riverine dissolved organic matter.

Dissolved organic matter (DOM) plays a central role in ecosystem health and carbon cycling. However, uncertainties remain regarding DOM molecular assembly mechanisms under varying organic carbon concentrations triggered by natural and anthropogenic factors. This study integrates geochemical analyses with molecular characterization of surface water and pore water DOM from seven rivers spanning diverse biomes and non-purgeable organic carbon (NPOC) gradients.

Effects of lithium nickel manganese cobalt oxide exposure on biological age acceleration: Insights from metabolomics.

The Lithium nickel manganese cobalt oxide (NCM) is a representative new energy material widely used in industry and daily life, but the systemic health influence of exposure to NCM remained largely unknown. This study aimed to reveal the impacts of NCM exposure on biological age acceleration (BAA) and metabolic alteration, as well as the mediating roles of metabolites. NCM exposure was assessed through three methods: external exposure, biomarker of exposure to NCM mixture (Li, Ni, Co, and Mn), with a particular focus on Li exposure.

Updating conservation metagenomics on the gut microbiome of threatened mammals.

During the past two decades, much work has characterized the composition and function of the gut microbiome on diverse wildlife in the framework of conservation metagenomics. While promising for conservation, translating theory into practice remains challenging and requires progress. Here, we summarize the progress of gut microbiomes of threatened mammals from ecology and evolution to conservation practice, and propose an integrative framework for future conservation metagenomics within which multi-dimension, multi-scale, multi-technology, and multi-omics can be comprehensively considered to advance our understanding of the patterns and underpinnings of wildlife gut microbiomes.

The Structural Deciphering of the α3 Helix Within ZmHsfA2'S DNA-Binding Domain for the Recognition of Heat Shock Elements in Maize.

Heat shock transcription factor (Hsf) plays a pivotal role in regulating plant growth, development, and stress responses. Hsf activates or represses target gene transcription by binding to the heat shock element (HSE) of downstream genes. However, the specific interaction sites between Hsf and the HSE in the promoter remain unclear.

Synergistic radical generation and electron transfer mechanisms in oxygen-deficient CoO: bridging defect concentration and catalytic efficiency in PMS-based AOPs.

A two-step pyrolysis procedure was carried out to precisely control the oxygen vacancy content in ZIF-67-derived CoO nanocubes. Higher concentrations of oxygen vacancies can significantly enhance the electron transfer ability of the material, thereby promoting the generation of ROS and enhancing the degradation of tetracycline (TC).

Disentangling the Genetic Architecture of Key Traits for Wheat Hybrid Seed Production.

Hybrid wheat offers significant benefits such as increased yield and high yield stability. Despite this, the commercial adoption of wheat hybrids has been limited, primarily due to the high cost of hybrid seed production. Improving its efficiency requires a deeper understanding of key traits influencing hybrid seed set and their genetic bases.

Self-Healing Injectable Double Cross-Linked Hydrogels and Oriented Network-Structured Aerogels as Foodborne Macromolecular Wound Healing Systems.

Acute wounds exhibit complex diversity, necessitating immediate hemostasis and tailored wound care strategies. To address these challenges, we developed protein- and polysaccharide-based hydrogel and aerogel dressings designed to adapt to the therapeutic demands of diverse clinical injury scenarios. Herein, a double cross-linked hydrogel based on type B gelatin (Gel), oxidized konjac glucomannan (OKGM), and borax was synthesized.

Precise formation of food applied Pickering droplets and microspheres using microfluidic technology.

Droplets and microspheres have broad applications in food fields. However, the droplets and microspheres prepared by traditional methods exhibit poor monodispersity, wide size distribution, and inhomogeneous morphology. In this study, we prepared Pickering droplets and microspheres via microfluidics to improve the controllability.

Uptake of the IMMPACT-recommended core outcome set in randomised controlled trials on chronic neck or shoulder pain: a cross-sectional study.

Objective: To analyse the uptake rate of Initiative on Methods, Measurement, and Pain Assessment in Clinical Trials (IMMPACT)-recommended core outcome set (COS) in randomised controlled trials (RCTs) on chronic neck or shoulder pain and its potential influencing factors.

Design: A cross-sectional study.

Setting: WHO International Clinical Trials Registry Platform, involving 15 registries.

Revealing Potential Therapeutic Targets in Gastric Cancer through Inflammation and Protein-Protein Interaction Hub Networks.

Gastric cancer (GC) ranks second in incidence and mortality among digestive system cancer, following colorectal cancer. Currently treatment options are limited, and the prognosis for GC remains poor. Four bulk RNA sequencing (RNA-seq) datasets and two single-cell RNA sequencing (scRNA-seq) datasets were downloaded from the Gene Expression Omnibus (GEO) database.

Impact of digital health management on clinical outcomes during post-PCI outpatient care in patients with acute coronary syndrome: study protocol for a multicentre, randomized controlled trial.

Introduction: Coronary heart disease is becoming more common in developing nations. Acute coronary syndrome (ACS) poses a major challenge to global health; however, access to cardiac rehabilitation and preventative measures is limited. There is an urgent need for affordable and widely accessible alternative delivery models to resolve this treatment gap.

Medium-Chain Chlorinated Paraffins Altered Neurobehavior and Induced Neuroinflammation Associated with Disordered Intestinal Homeostasis.

Chlorinated paraffins, derivatives of chlorinated -alkanes, are widely used in agricultural and industrial applications. With the restriction of short-chain chlorinated paraffins (SCCPs), the use of medium-chain chlorinated paraffins (MCCPs) has sharply increased. MCCPs have recently been detected in foods and animal brains, raising concerns about their health effects.

Nasopharyngeal Carcinoma and Head and Neck Cancer in Type 2 Diabetes after SGLT2I, DPP4I, GLP1a Use.

Nasopharyngeal carcinoma (NPC) remains endemic in Asia, which risk factors were distinct from other head and neck (H&N) cancers. Anti-diabetic drugs have been proposed to reduce the risk of NPC. The associations between sodium glucose cotransporter 2 inhibitors (SGLT2I) versus dipeptidyl peptidase-4 inhibitors (DPP4I) and the risks of NPC and H&N cancer amongst type-2 diabetes mellitus (T2DM) patients remains unknown.

Soil C mineralization and C-Fe coupling: a trade-off between Fenton reaction and microbial activity under straw mulching in banana orchards.

Straw mulching influences soil carbon (C) cycling by adding organic matter and modifying the soil structure, with soil iron (Fe)-associated C (Fe-OC) and reactive oxygen species (ROS) playing critical roles in this process. However, the dynamics in soil Fe-OC and ROS following mulching in banana orchards remain unclear. This study investigated the impact of 3 years of straw mulching on soil Fe-OC, ROS, and soil C mineralization.

Activation of G protein-coupled estrogen receptor alleviates the abnormal changes of synaptic plasticity in the anterior insula of temporal lobe epilepsy rats through RhoA/Rock2 pathway.

Temporal lobe epilepsy (TLE) is the most common type of refractory epilepsy, characterized by highly synchronized abnormal neuronal discharge. The insula cortex (IC) serves as a key "node" in the TLE transmission network, and the anterior insula (AI) is a critical gatekeeper to executive control; however, the pathological changes of the IC/AI have been overlooked. GPER1 is a G protein coupled estrogen receptor anchored by PSD95 to the plasma membrane of dendritic spine (DS), participating in the regulation of DS plasticity.

China Multi-Center Cohort Study on Risk Evaluation of Arrhythmogenic Cardiomyopathy: The ChinaCORE ACM Registry.

Background: Arrhythmogenic cardiomyopathy (ACM) patients in China exhibit unique genetic and clinical characteristics. There is a lack of prognostic models specific to Chinese ACM patients.

Objectives: This study aims to establish a large, national ACM patient cohort with uniformly collected, high-quality data for future risk prediction.

Transcriptome analysis reveals PTBP1 as a key regulator of circRNA biogenesis.

Background: Circular RNAs (circRNAs) are a class of non-coding RNAs generated through back splicing. High expression of circRNAs is often associated with numerous abnormal cellular biological processes. However, the regulatory factors of circRNAs are not fully understood.