Chromosomal Translocations Detection in Cancer Cells Using Chromosomal Conformation Capture Data.

Complex chromosomal rearrangements such as translocations play a critical role in oncogenesis. Translocation detection is vital to decipher their biological role in activating cancer-associated mechanisms. High-throughput chromosomal conformations capture (Hi-C) data have shown promising progress in unveiling the genome variations in a disease condition.

Stable magnetic CoZn/N-doped polyhedron with self-generating carbon nanotubes for highly efficient removal of bisphenols from complex wastewaters.

Bisphenols have extensively been found in various environmental matrices and caused public concerns due to their endocrine-disrupting potential. Herein, we developed a ZIF-67@ZIF-8-derived CoZn/nitrogen-doped carbon (CoZn/NC) as a robust adsorbent for bisphenols in wastewaters. The self-generating carbon nanotubes and the open metal sites provided sufficient adsorption sites.

Genomic insights into historical improvement of heterotic groups during modern hybrid maize breeding.

Single-cross maize hybrids display superior heterosis and are produced from crossing two parental inbred lines belonging to genetically different heterotic groups. Here we assembled 1,604 historically utilized maize inbred lines belonging to various female heterotic groups (FHGs) and male heterotic groups (MHGs), and conducted phenotyping and genomic sequencing analyses. We found that the FHGs and MHGs have undergone both convergent and divergent changes for different sets of agronomic traits.

Characteristics and Regulating Roles of Wheat TaHsfA2-13 in Abiotic Stresses.

Heat shock transcription factor (Hsf) exists widely in eukaryotes and responds to various abiotic stresses by regulating the expression of downstream transcription factors, functional enzymes, and molecular chaperones. In this study, , a heat shock transcription factor belonging to A2 subclass, was cloned from wheat () and its function was analyzed. encodes a protein containing 368 amino acids and has the basic characteristics of Hsfs.

[Association of sudden sensorineural hearing loss and its prognosis with the brain white matter hyperintensity].

To explore the relationship between white matter hyperintensity（WMH） and sudden sensorineural hearing loss（SSHL） and evaluate the influence of WMH on the prognosis of SSHL. Fifty hospitalized unilateral SSHL patients and 50 age and gender matched routine physical examination individuals without SSHL history from June 2019 to June 2020 were included for a case-control study. All included subjects underwent 3.

Circ-GSK3B up-regulates GSK3B to suppress the progression of lung adenocarcinoma.

GSK3B is the mRNA form of glycogen synthase kinase 3 beta (GSK-3β), which is a critical repressor of Wnt/β-catenin signaling pathway and generally inhibited in cancer cells. Plenty of researches have disclosed that circular RNAs, namely circRNAs exert important functions in the progression of various human malignancies including lung adenocarcinoma (LUAD). Therefore, we attempted to explore whether there existed certain circRNAs that could mediate LUAD development by regulating GSK3B expression and Wnt/β-catenin pathway.

Telomerase positive CTCs with PSMA high expression associated with prostate cancer metastasis.

Background: Whether circulating tumor cells (CTCs) with prostate-specific membrane antigen (PSMA) high expression was related to the metastatic progress in prostate cancer (PCa) remains explored. This study aimed to provide evidence to elucidate this relationship via the telomerase reverse transcriptase (TERT)-based CTC detection method.

Methods: A total of 71 patients were enrolled and divided into the local PCa group (n=44) and metastatic PCa group (n=27).

High-efficiency enzyme-free catalyzed hairpin assembly-mediated homogeneous SERS and naked-eyes dual-mode assay for ultrasensitive and portable detection of mycotoxin.

We developed an aptamer recognition-trigged enzyme-free catalyzed hairpin assembly (CHA) assisted signal amplification homogeneous naked-eyes and surface-enhanced Raman scattering (SERS) dual-mode sensor for highly sensitive and portable detection of Aflatoxin B (AFB) in food samples. The recognition of AFB by aptamer induced the generation of HP1-AFB complexes, which hybridized with Ag-labeled hairpin DNA (HP2) and released Ag, subsequently initiating the enzyme-free CHA reaction by a designed helper DNA (HP3) to form double-stranded DNA (HP2-HP3) and accompanied by the release of HP1-AFB complexes. The released HP1-AFB complexes were recognized by HP2 and HP3 again to trigger cascade recycling amplification and resulted in the generation of a larger number of free Ag and dsDNA.

Research on performance and dynamic competency evaluation of bid evaluation experts based on weight interval number.

Purpose/significance: In the past many years, some scholars have studied bid evaluation experts, such as the behavior of bid evaluation experts. However, previous research ignores the performance and competency of bid evaluation experts, so this paper aims to provide a theoretical basis for incentive and constraint mechanism and hierarchical or dynamic management of bid evaluation experts by implementing performance and dynamic competency evaluation of bid evaluation experts.

Method/process: Firstly, the evaluation index system of performance and dynamic competency of bid evaluation experts is preliminarily constructed by referring to relevant literature, and then the constructed evaluation index was modified and improved by consulting relevant stakeholders' experts.

Metabolomics-based evidence of the hypoglycemic effect and alleviation of diabetic complications by fruit in diabetic mice.

The hypoglycemic and metabolic effects of fruit were studied in diabetic mice, and its potential mechanisms of hypoglycemic activity and its alleviation of diabetic complications were explored using a metabolomics approach. The histopathological effect of fruit was characterized by hematoxylin and eosin histological staining. Dynamic fasting blood glucose (FBG), postprandial glucose (PPG), body weight, and biochemical parameters, including hepatic-renal function and lipid levels, were monitored to confirm the hypoglycemic activity and attenuation effect.

Mercury emission characteristics and mechanism in the raw mill system of cement clinker production.

Mercury pollution has attracted worldwide attention due to its toxicity, bioaccumulation and persistence. Cement clinker production is the top emitter of atmospheric mercury in China and the emissions from raw mill systems account for about 85% of all emissions. However, the mercury emission characteristics and mechanisms as a function of time during an operation cycle are still unclear.

A Territory-Wide Study of Arrhythmogenic Right Ventricular Cardiomyopathy Patients from Hong Kong.

Background: Arrhythmogenic right ventricular cardiomyopathy/dysplasia (ARVC/D) is a hereditary disease characterized by fibrofatty infiltration of the right ventricular myocardium that predisposes affected patients to malignant ventricular arrhythmias, dual-chamber cardiac failure and sudden cardiac death (SCD). The present study aims to investigate the risk of detrimental cardiovascular events in an Asian population of ARVC/D patients, including the incidence of malignant ventricular arrhythmias, new-onset heart failure with reduced ejection fraction (HFrEF), as well as long-term mortality.

Methods And Results: This was a territory-wide retrospective cohort study of patients diagnosed with ARVC/D between 1997 and 2019 in Hong Kong.

The Involvement of Antioxidant Enzyme System, Nitrogen Metabolism and Osmoregulatory Substances in Alleviating Salt Stress in Inbred Maize Lines and Hormone Regulation Mechanisms.

Salt stress inhibited the growth of maize. B46 and NC236 were chosen as materials and NaCl concentrations (0, 55, 110, 165, and 220 mmol L) were set. We found the activities of SOD, POD, CAT, APX, GR, MDHAR, and DHAR decreased under NaCl stress.

Low background interference SERS aptasensor for highly sensitive multiplex mycotoxin detection based on polystyrene microspheres-mediated controlled release of Raman reporters.

Mycotoxin contamination is a serious global issue in food safety. The accurate detection of mycotoxins in complex samples, particularly via a portable detection system that realizes multi-analyte detection, remains a great challenge. Here, a polystyrene (PS)-mediated surface-enhanced Raman scattering (SERS) aptasensor was designed for ultrasensitive and simultaneous detection of multiple mycotoxins in food samples.

Deep Learning-Based Segmentation of Peach Diseases Using Convolutional Neural Network.

Peach diseases seriously affect peach yield and people's health. The precise identification of peach diseases and the segmentation of the diseased areas can provide the basis for disease control and treatment. However, the complex background and imbalanced samples bring certain challenges to the segmentation and recognition of lesion area, and the hard samples and imbalance samples can lead to a decline in classification of foreground class and background class.

An aptamer-assisted biological nanopore biosensor for ultra-sensitive detection of ochratoxin A with a portable single-molecule measuring instrument.

Biological nanopore-based single-molecule detection technology has shown ultrahigh sensitivity to various target analyte. But the detection scope of interesting targets is limited due to the lack of effective signal conversion strategies. In addition, conventional nanopore detection instruments are cumbersome, resulting nanopore detection can only be performed in laboratory.

Heat stress response in Chinese cabbage ( L.) revealed by transcriptome and physiological analysis.

High temperatures have a serious impact on the quality and yield of cold-loving Chinese cabbage, which has evolved to have a unique set of stress mechanisms. To explore the relationship between these mechanisms and the heat-tolerance of Chinese cabbage, the physiological indicators of the heat-tolerant '268' line and heat-sensitive '334' line were measured. Under heat stress, the proline (Pro), soluble sugar (SS), and superoxide dismutase (SOD) indexes of the '268' line increased significantly.

Optimization of ethanol-extracted lignin from palm fiber by response surface methodology and preparation of activated carbon fiber for dehumidification.

Lignin is a renewable bioresource that can be used for a variety of value-added applications. However, the effective separation of lignin from lignocellulosic biomass remains an ongoing challenge. In this study, lignin was extracted from waste palm fiber and successfully converted into a dehumidifying material.

Transition Metals Embedded Two-Dimensional Square Tetrafluorotetracyanoquinodimethane Monolayers as a Class of Novel Electrocatalysts for Nitrogen Reduction Reaction.

The combination of transition metal (TM) atoms and high electron affinity organic framework tetrafluorotetracyanoquinodimethanes (FTCNQs) makes the TM-embedded two-dimensional (2D) square FTCNQ monolayers (TM-sFTCNQ) possible to have excellent characteristics of single-atom catalysts and 2D materials. For the first time, the TM-sFTCNQ monolayers have been considered for application in the electrocatalytic nitrogen reduction reaction (eNRR) field. Through high-throughput screening, the catalytic performance of 30 TM-sFTCNQ (TM = 3d∼5d TMs) monolayers for eNRR was comprehensively evaluated.

Elevated Gaseous Oxidized Mercury Revealed by a Newly Developed Speciated Atmospheric Mercury Monitoring System.

Gaseous oxidized mercury (Hg) monitoring is one of the largest challenges in the mercury research field, where existing methods cannot simultaneously satisfy the measurement requirements of both accuracy and time precision, especially in high-particulate environments. Here, we verified that dual-stage cation exchange membrane (CEM) sampler is incapable of gaseous elemental mercury (Hg) uptake even if particulate matter is trapped on CEM, whereas the Hg capture efficiency of the sampler is more than 90%. We then developed a Cation Exchange Membrane-Coupled Speciated Atmospheric Mercury Monitoring System (CSAMS) by coupling the dual-stage CEM sampler with the commercial Tekran 2537/1130/1135 system and configuring a new sampling and analysis procedure, so as to improve the monitoring accuracy of Hg and ensure the simultaneous measurement of Hg, Hg, and Hg in 2 h time resolution.

Genetic dissection of heterotic loci associated with plant weight by Graded pool-seq in heading Chinese cabbage (Brassica rapa).

Four heterotic QTL and a heterozygous segment for plant weight were identified by Graded Pool-Seq, QTL-seq and traditional genetic linkage analysis in heading Chinese cabbage. Heading Chinese cabbage (Brassica rapa L. spp.

Click Isotope Mass Probe for Highly Selective Determination of Trace Steroid Hormones in Food Samples.

Matrix effects are a great challenge for the quantitative analysis of complex food samples by liquid chromatography-tandem mass spectrometry analysis (LC-MS/MS). Stable isotope labeling (SIL) has been widely used as an effective strategy to eliminate matrix effects. Herein, a copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click-reaction-based SIL method was proposed for a highly sensitive and selective determination of six synthetic steroid hormones in three different food samples (milk, yogurt, and eggs) by high-performance liquid chromatography (HPLC)-MS/MS.

Prognostication of two-dimensional transition-metal atoms embedded rectangular tetrafluorotetracyanoquinodimethane single-atom catalysts for high-efficiency electrochemical nitrogen reduction.

Extensive investigations on the electrocatalytic nitrogen reduction reactions (eNRR) and the high-efficiency single-atom catalysts (SACs) have increasingly given us confidence in intensive arrival of nitrogen (N) fixation into ammonia (NH) under ambient conditions in the future, which prompts us to speed up the exploration for highly active SACs for eNRR. Excellent SACs in eNRR should have three advantages: high selectivity, low overpotential, and high stability. Based on these aspects, we employed high-throughput screening method and first-principles calculations to study the catalytic performance of 30 transition-metal atoms (TMs) embedded rectangular tetrafluorotetracyanoquinodimethane (denoted as TM-rFTCNQ) monolayers (TM = 3d, 4d, and 5d series transition metal atoms) for the eNRR process, and four potential catalysts, i.

Transcriptome analysis reveals anthocyanin regulation in Chinese cabbage (Brassica rapa L.) at low temperatures.

Chinese cabbage that prefers cold conditions is also affected by low-temperature stress, such as the accumulation of leaf anthocyanins. Research on anthocyanin biosynthesis and regulation mechanisms has made great progress. However, research on anthocyanin accumulation for resistance to biological and non-biological stress is still lacking.

Fetuin-A Promotes 3-Dimensional Growth in LNCaP Prostate Cancer Cells by Sequestering Extracellular Vesicles to Their Surfaces to Act as Signaling Platforms.

The present studies were conducted to evaluate key serum proteins and other components that mediate anchorage-independent growth (3-D growth) of LNCaP prostate cancer cells as spheroids. The cells were cultured on ultra-low attachment plates in the absence and presence of fetuin-A and with or without extracellular vesicles. The data show that fetuin-A (alpha 2HS glycoprotein) is the serum protein that mediates 3-D growth in these cells.