Integration of cell-type resolved spatial proteomics and transcriptomics reveals novel mechanisms in early ovarian cancer.

High-grade serous carcinoma (HGSC) is the most common ovarian cancer subtype, typically diagnosed at late stages with poor prognosis. Understanding early molecular events driving HGSC progression is crucial for timely detection and development of effective treatment strategies. We performed and integrated spatial cell-type resolved proteomics and paired transcriptomics across 25 women with precursor lesions of the fallopian tube and/or HGSC.

Inflammation awakens dormant cancer cells by modulating the epithelial-mesenchymal phenotypic state.

The awakening of dormant disseminated cancer cells appears to be responsible for the clinical relapses of patients whose primary tumors have been successfully cured months and even years earlier. In the present study, we demonstrate that dormant breast cancer cells lodged in the lungs reside in a highly mesenchymal, nonproliferative phenotypic state. The awakening of these cells is not triggered by a cancer cell-autonomous process.

Lipid overload meets S-palmitoylation: a metabolic signalling nexus driving cardiovascular and heart disease.

S-palmitoylation has emerged as a critical integrator of lipid overload and cardiovascular dysfunction. Disordered lipid metabolism inundates endothelial cells, vascular smooth muscle cells and macrophages with triglyceriderich lipoproteins, oxidized LDL and saturated fatty acids, expanding the intracellular palmitoylCoA pool and perturbing redox balance. Protein Spalmitoylation, the reversible attachment of palmitate to cysteine residues, converts excess palmitoylCoA into broad alterations in signalling and membrane dynamics.

Factors affecting preoperative sentinel lymph nodes contrast-enhanced ultrasound in breast cancer.

Background: Preoperative evaluation of sentinel lymph nodes is very important in breast cancer patients. This study aimed to explore factors affecting the result of preoperative percutaneous contrast-enhanced ultrasound for sentinel lymph nodes (SLN-CEUS) using Sonovue.

Methods: A total of 176 patients with breast cancer who underwent preoperative SLN-CEUS to trace axillary sentinel lymph nodes were included.

Estimation of Seasonal Net Carbon Sequestration Under Noncontinuous Flooding in Rice Fields.

Non-continuous flooding (NCF) in rice was recently reported to improve the field-scale, seasonal carbon balance. In this response article, we clarify our system boundaries (seasonal, field-scale), address the role of yield carbon and microbial indicators, and add sensitivity checks with expanded data. Across these checks, NCF consistently reduces methane without penalizing yield, supporting our original conclusions.

Sleep Physiology in Spine Disease: A Comparative Study of Cervical and Lumbar Patients.

Study Design: Prospective cohort study.

Objective: We aim to compare Fitbit-collected sleep metrics between patients with cervical and lumbar spine diseases and explore correlations between these metrics and patient-reported outcome measures (PROMs) and self-reported sleep.

Summary Of Background Data: Pain, fatigue, and postural problems often lead to sleep disturbances in patients with spine disease.

Low-order digital controller design based on l parametric stability margin.

The uncertainties inherent in engineering control systems are generally intricate and multifaceted. Sometimes, the model parameters of the plants may even fluctuate significantly. To address this undesirable scenario and ensure system stability under such conditions, the controller parameters with the largest parametric stability margin in the stabilizing set should be selected.

Drop foot post-ECMO, subsequently complicated by third-degree burns: A case report based on user portrait and health management journey map.

Rationale: The long-term complications of extracorporeal membrane oxygenation (ECMO) have not been well documented, especially the rare lower extremity drop foot (LEDF). Understanding the mechanisms and management of such complications is critical to improving patient outcomes. What is the role of patient-based on user portrait and health management journey map (HMJM) for rehabilitation management of patients with post-intensive care syndrome (PICS)?

Patient Concerns: We reported a case of a patient who developed LEDF after receiving ECMO for severe heart failure.

A Small Molecule Selectively Targets N-Myc to Suppress Neuroblastoma Cancer Progression.

Neuroblastoma, a prevalent and lethal extracranial solid tumor in childhood, remains a significant challenge in pediatric oncology worldwide. High-risk neuroblastoma (HR-NB) is particularly aggressive and linked to a poor prognosis due to the limited availability of effective treatments. The aberrant amplification of the gene is a critical genetic alteration observed in neuroblastoma conferring poorer clinical outcomes.

Effect of Intravenous Lidocaine (1.0 mg/Kg) on Propofol ED50 and ED95 for Successful Double-Lumen Laryngeal Mask Airway Placement in Hysteroscopy Patients: A Randomized Controlled Trial.

Purpose: It has been demonstrated that administration of intravenous lidocaine promotes laryngeal mask airway (LMA) placement. This research aimed to evaluate the effect of intravenous lidocaine at a dosage of 1.0 mg/kg on the 50% and 95% effective doses (ED50 and ED95) of propofol required for the successful placement of a double-lumen LMA during propofol-fentanyl-based anesthesia in patients undergoing hysteroscopy.

Efficacy and safety of Huachansu capsule as adjuvant therapy for breast cancer: A systematic review and meta-analysis of randomized controlled trials.

Ethnopharmacological Relevance: Huachansu capsule (HCSC) is a traditional Chinese patent medicine derived from toad venom (Bufo bufo gargarizans Cantor). In China, HCSC has been widely used as a therapeutic agent for breast cancer (BC). It exerts antitumor effects by inducing apoptosis of BC cells, disrupting the tumor cell cycle, and regulating the immune system.

Assembly of a high-quality reference genome and characterization of a chemical-mutagenized library of an elite soybean cultivar Tianlong 1.

Soybean (Glycine max L.) is a globally vital crop for oil production and food security. High-quality genomic resources are instrumental for both functional genomics and breeding.

Single-cell technology reveals the crosstalk between tumor cells and immune cells: driving immune signal transduction and inflammation-mediated cardiac dysfunction in the tumor microenvironment of colorectal cancer.

Background: Colorectal cancer (CRC) is a heterogeneous illness influenced by intricate tumor-immune interactions and characterized by a dismal prognosis. Macrophage-mediated immunological signaling facilitates tumor proliferation and may associate inflammation in the tumor microenvironment (TME) of CRC with negative outcomes. Notwithstanding therapeutic advancements, resistance to treatment remains a significant obstacle.

A wearable and wireless electrochemical device based on Cu-BTC MOF for pyocyanin detection and wound healing.

The prevalent and serious antimicrobial resistance in Pseudomonas aeruginosa (PA) infections has emerged as a critical public health challenge worldwide. Pyocyanin (PYO), an important virulence component produced by PA, is considered as an ideal indicator for identifying infections linked to this pathogen. This study evaluated ten metal-organic frameworks (MOFs) as signal amplifiers for the sensitive detection of PYO.

Multi level synergistic regulation of phosphorus slow-release performance through LDH modified biochar-based slow-release fertilizer.

Addressing global phosphorus resource scarcity and low utilization efficiency of traditional phosphorus fertilizers, this study presents an innovative strategy for modifying biochar-based slow-release phosphorus fertilizer (BSPF) with Mg-Al layered double hydroxides (LDH-BSPF), to enhance phosphorus slow-release performance through multi-level synergistic regulation. A novel slow-release system was fabricated via co-pyrolysis technology integrating biomass, phosphorus sources, and LDH. The effects of Mg/Al molar ratios (2:1-5:1) and pyrolysis temperatures (400-600 °C) on material structural properties were systematically investigated.

Association between preoperative activity levels and postoperative complications in adult spinal deformity surgery.

Purpose: Adult spinal deformity (ASD) is a condition associated with significant pain, disability, and high complication rates after surgery, reaching 38-71%. These complications significantly impact recovery, which often takes over a year. Efforts to mitigate complications have focused on preoperative risk stratification using factors such as frailty and patient demographics.

Model for Predicting Central Lymph Node Metastasis in Papillary Thyroid Carcinoma: A Study Based on Ultrasound Viscosity Imaging.

Objective: To investigate the association between ultrasound (US) viscosity imaging and central lymph node metastasis (CLNM) in papillary thyroid carcinoma (PTC) and to develop a new model for predicting CLNM based on patient clinical data, grey-scale US characteristics and US viscosity imaging parameters.

Materials And Methods: This prospective study enrolled patients with PTC who underwent preoperative US viscosity from June 2024 to September 2024. Using histological results as the reference standard, optimal cutoff values for the viscosity parameter were established to predict central lymph node metastasis (CLNM).

Rotational Stability of Three Toric Intraocular Lens Platforms in Patients Undergoing Repositioning Surgery.

Purpose: To evaluate the rotational stability of three toric intraocular lens (TIOL) platforms in patients undergoing repositioning surgery.

Methods: This comparative retrospective study enrolled eyes that underwent cataract surgery and implanted with a AcrySof, AT TORBI/LISA, or TECNIS TIOL. Patients who experienced TIOL misalignment ≥10° postoperatively and underwent repositioning surgery were included.

Effects of different electroacupuncture/ transcutaneous electrical acupoint stimulation parameters on the pregnancy outcomes of in vitro fertilisation-embryo transfer: a systematic review and meta-analysis.

Background: Extensive progress has been made in improving pregnancy outcomes for in vitro fertilisation and embryo transfer (IVF-ET) patients through the use of electroacupuncture (EA) and transcutaneous electrical acupoint stimulation (TEAS). However, a clear and suitable recommendation for the parameter selection scheme of EA/TEAS remains elusive.

Objective: To evaluate evidence-based conclusions of different EA/TEAS parameters on improvement of pregnancy outcomes in patients undergoing IVF-ET and evaluate other factors that may affect pregnancy outcomes.

Impact of social fairness perception on sense of social security in China's COVID-19 pandemic: the mediating role of political trust.

Introduction: Against the backdrop of the COVID-19 pandemic, emerging threats previously obscured were revealed and instilled a profound sense of insecurity across the globe. The exacerbation of unequal access to essential resources during the pandemic, particularly in rural-urban divides (e.g.

Function of chemokines in embryo implantation.

Embryo implantation centrally involves the maternal immune system's specific acceptance of the semi-allogeneic embryo. Despite advances in assisted reproduction, implantation failure remains a key bottleneck limiting clinical pregnancy rates, with its mechanisms a significant challenge in reproductive immunology. Recent studies reveal that chemokine-mediated dynamic remodeling of the immune microenvironment is central to maintaining maternal-fetal immune homeostasis.

Laccase immobilized on amino acid ionic liquid-modified magnetic lignin nanoparticles and its degradation of 2,4-dichlorophenol.

Immobilized laccase demonstrates significant potential for 2,4-dichlorophenol (2,4-DCP) degradation in environmental remediation applications. However, challenges persist in optimizing carrier materials for enzyme immobilization. In this study, a magnetic lignin-based nanocomposite (FeO-SL) was synthesized and further functionalized with amino acid-derived ionic liquids (AAILs).

Langqing Meiduo Jiujie pills treatment attenuates acute liver injury in animals by regulating anti-oxidative stress and liver metabolism.

Introduction: Langqing Meiduo Jiujie pills (LQMDJJP), a Tibetan formula, has a history of more than 400 years of clinical use. However, there has been no report on the scientific basis of its dosage or its mechanism of action in treating acute liver injury. To investigate the optimal clinical dosage of LQMDJJP, to examine the differences in differential metabolites in liver tissue following treatment with LQMDJJP, and to explore the mechanism through which LQMDJJP acts in the treatment of acute liver injury.

Establishing objective markers of physical activity to identify early improvement after lumbar spine surgery.

Objective: Despite growing interest in using biometric measures as objective tools to monitor recovery after spine surgery, there is sparse guidance on appropriate thresholds to evaluate perioperative changes in these domains. The authors defined thresholds of activity and heart rate (HR) measures that mark improved early recovery and evaluated their association with longer-term surgical outcomes.

Methods: A prospective observational cohort study recruited patients undergoing elective surgery for lumbar degenerative spine disease at a single institution from February 2021 to June 2023.

Synergistic OH/H modulation over amorphous GdO-Pt interfaces for efficient alkaline hydrogen oxidation reaction electrocatalysis.

The integration of low-loading Pt sites with amorphous GdO on a carbon support in the as-prepared Pt-GdO/C not only significantly modifies the electronic structure but also precisely modulates the binding strengths of various adsorbates (H*, OH*, and CO*). This synergistic effect leads to substantially enhanced hydrogen oxidation reaction (HOR) performance in alkaline media, as well as practical applications in anion exchange membrane fuel cells.