Integrin β3 dysregulation impairs megakaryopoiesis and microparticle production via disrupting ROCK-dependent cytoskeletal dynamics.

Background: Megakaryocyte (MK) fragmentation into proplatelets (PPTs) and microparticles (MKMPs) is well established, yet the mechanisms underlying MKMP generation remain unclear.

Objectives: In order to investigate the role of integrin β3 and cytoskeletal dynamics during megakaryopoiesis and explore potential therapeutic targets for thrombocytopenia.

Methods: Proplatelet formation and MKMP release were evaluated both in vivo and in vitro under integrin β3 receptor impaired environment.

The immunoregulator β-glucan produces antidepressant effects through microglia-mobilized astrocytic P2Y1R-BDNF signaling in the dentate gyrus.

β-Glucan, a polysaccharide from Saccharomyces cerevisiae with immunomodulatory activities that may not trigger pro-inflammatory responses in microglia, has been reported to show rapid antidepressant effects in chronically stressed animals by restoring microglial function in the dentate gyrus. However, the mechanisms underlying this effect of β-glucan are still largely unclear. Considering the importance of astrocytic purinergic 2Y1 receptors (P2Y1Rs) and brain-derived neurotrophic factor (BDNF) in the antidepressant effects of microglial stimulation, we hypothesize that β-glucan produces antidepressant effects by mobilizing astrocytic P2Y1R-triggered BDNF signaling in the hippocampus.

Deciphering disease-specific glycosylation: unraveling diabetes subtypes through serum glycopattern.

Latent autoimmune diabetes in adults (LADA) is a slowly progressing form of diabetes that develops in adulthood, characterized by autoimmune destruction of pancreatic β-cells and subsequent insulin deficiency, akin to type 1 diabetes (T1D). Due to its shared genetic, immunological, and metabolic features with both T1D and type 2 diabetes (T2D), LADA is frequently misdiagnosed and inappropriately treated as T2D. To address this, we developed the A.

Neuro-immuno-stromal context in colorectal cancer: An enteric glial cell-driven prognostic model via machine learning predicts survival, recurrence, and therapy response.

Background: Enteric glial cells (EGCs) have been implicated in colorectal cancer (CRC) progression. This study aimed to develop and validate a prognostic model integrating EGC- and CRC-associated gene expression to predict patient survival, recurrence, metastasis, and therapy response.

Methods: Bulk and single-cell RNA sequencing data were analyzed, and a machine learning-based model was constructed using the RSF random forest algorithm.

FGF9-FGFR2 Signaling via Osteocytes-Preosteoblasts Crosstalks to Mediate Mechanotransduction-Driven Intramembranous Osteogenesis in the Underdeveloped Maxilla.

Maxillary underdevelopment is a critical component of skeletal Class III malocclusion, closely linked to altered biomechanical signaling. Mechanical stimulation through early facemask protraction can effectively promote maxillary growth, yet the underlying mechanotransduction mechanisms remain unclear. In this study, fibroblast growth factor 9 (FGF9) is identified as a key biomechanical responder in maxillary development.

METTL3 Silencing Suppresses Cardiac Fibrosis Post Myocardial Infarction via m6A Modification of SMOC2.

Cardiac fibrosis, especially in the infarct border zone, leads to decreased cardiac compliance, impaired systolic and diastolic function, resulting in heart failure. M6A methylation plays a role in fibrosis development. However, its underlying mechanism remains poorly understood.

Unraveling cellular dynamic changes in tumor evolution induced by long-term low dose-rate radiation.

Background: In recent years, there has been a steady increase in professionals engaged in radioactive work. The biological impacts of long-term exposure to low dose-rate radiation remain elusive, as there is a dearth of systematic research in this field.

Methods: BEAS-2B cells were used to establish a cell model with continuous passaging after radiation exposure, which was subsequently subjected to in vivo tumorigenesis assays and in vitro malignant phenotype experiments.

Efficacy and safety of intranasal corticosteroids combined with mucoactive drugs for chronic rhinosinusitis.

Background: Chronic rhinosinusitis (CRS) leads to a burden in life and economy. Better therapies need to be explored.

Objective: This stage I study aims to explore the efficacy and safety of intranasal corticosteroids combined with mucoactive drugs for CRS.

BHLHE40 Cooperates with GATA2/3 to Control Human Syncytiotrophoblast Lineage Differentiation.

Syncytiotrophoblasts (STBs) constitute one of the core components of the placenta, responsible for synthesizing pregnancy-sustaining hormones such as human chorionic gonadotropin (HCG). Deficient syncytialization of cytotrophoblasts affects the hormonal secretion and placental development, contributing to pregnancy-associated disorders, including spontaneous miscarriage. To date, the molecular mechanisms, particularly the role of transcription factors (TFs), in STB lineage specification remain incompletely understood.

Unlocking New Treatment Horizons for Celiac Disease: PRKCD Revealed as a Promising Target through Mendelian Randomization.

Introduction: Celiac Disease (CeD) is a serious, lifelong autoimmune condition. There remains a significant unmet medical need for effective pharmacological treatments for CeD.

Methods: We utilized summary statistics for 2,888 druggable genes from the eQTLGen Consortium and the FinnGen Consortium for CeD.

Hydrogel adhesives with a hydrodynamically induced liquid-solid transition for annular fissure sealing and inflammation modulation following microdiscectomy.

Background: Intervertebral disc (IVD) herniation is a major cause of low back pain and disability, with microdiscectomy being the standard surgical treatment. However, microdiscectomy fails to address annulus fibrosus (AF) defects, increasing the risk of recurrent herniation. Current therapeutic strategies for this condition remain limited in efficacy.

A multi-module enhanced YOLOv8 framework for accurate AO classification of distal radius fractures: SCFAST-YOLO.

Introduction: CT-based classification of distal ulnar-radius fractures requires precise detection of subtle features for surgical planning, yet existing methods struggle to balance accuracy with clinical efficiency. This study aims to develop a lightweight architecture that achieves accurate AO (Arbeitsgemeinschaft für Osteosynthesefragen) typing[an internationally recognized fracture classification system based on fracture location, degree of joint surface involvement, and comminution, divided into three major categories: A (extra-articular), B (partially intra-articular), and C (completely intra-articular)] while maintaining real-time performance. In this task, the major challenges are capturing complex fracture morphologies without compromising detection speed and ensuring precise identification of small articular fragments critical for surgical decision-making.

Analysis of gender- and age-stratified asthma burden: forecasting prevalence trends in 2030.

Background: Asthma poses a global health challenge, requiring an understanding of its burden to guide policy. Using GBD 2021 data, this study aims to assess the burden of asthma worldwide.

Methods: We extracted data on asthma prevalence, incidence, years lived with disability (YLDs), and disability-adjusted life-years (DALYs) from GBD for the period 1990-2021.

PD-L1 neutrophils mediate immune regulation of CD8 T cells in halo nevi.

Background: Halo nevi are clinically common and are characterized by a circle of leukoderma around the central melanocytic nevus. Studies have shown that the pathogenesis of halo nevi is similar to that of vitiligo and is associated with the role of CD8⁺ T lymphocytes in melanocyte destruction. Histopathological findings have revealed neutrophil infiltration in halo nevi; however, the specific immune mechanisms involving neutrophils have not been thoroughly investigated.

Targeted metabolomics reveals dysregulated tryptophan metabolism in retinitis pigmentosa.

Retinitis pigmentosa (RP) is an inherited retinal degenerative disease characterized by progressive retinal pigment epithelium dysfunction and photoreceptor apoptosis, yet its pathogenesis remains unclear and no cure exists. Emerging evidence implicates the role of tryptophan metabolism in neuroinflammatory processes, prompting our investigation of serum tryptophan metabolites in RP patients versus healthy controls. Through targeted metabolomic profiling and clinical characterization, including age of onset, best corrected visual acuity (BCVA) and retinal thickness, we identified significant alterations in RP patients: marked decreases in cinnabarinic acid, xanthurenic acid, quinolinic acid and indole-3-carboxaldehyde (all p < 0.

Reduced TIGITCD56NK cells associate with disease progression and impaired immune regulation in primary Sjögren's syndrome.

Objectives: We aimed to explore the expression and clinical significance of TIGITCD56NK cells within the peripheral blood of patients with primary Sjögren's syndrome (pSS) in this study, as well as to examine the role of TIGIT in modulating NK cell function in individuals with pSS.

Methods: The percentage of TIGITCD56NK cells was detected in the peripheral blood of 76 individuals with pSS and 63 healthy controls (HCs) using flow cytometry. The percentage of TIGITCD56NK cells across various clinical features, laboratory parameters, and between active and inactive patients was analyzed.

Comparing long-term survival prognosis with surgery-based systemic treatment of gastrointestinal stromal tumors: A population-based study.

Background: To date, the optimal sequence of systemic therapy (ST) and surgical resection for gastrointestinal stromal tumors (GISTs) remains unknown. This study aimed to compare the long-term outcomes of different ST approaches when combined with surgery.

Subject And Methods: Data on GISTs were retrospectively collected from the Surveillance, Epidemiology, and End Results database.

Recent Advances of Aggregation-Induced Emission Luminogens in Atherosclerotic Cardiovascular Disease.

Atherosclerotic cardiovascular disease (ASCVD), the primary cause of mortality worldwide, is distinguished by the development of atherosclerotic plaques due to lipid accumulation in the artery wall, ultimately resulting in a range of severe cardiovascular and cerebrovascular complications. In recent years, aggregation-induced emission luminogens (AIEgens), which possess the advantages of fluorescence enhancement in aggregated states, exceptional photostability, and finely tunable biocompatibility, have emerged as a promising candidate for the diagnosis and treatment of ASCVD. This review systematically summarizes the research advancements of AIEgens in ASCVD over the past five years, mainly including the properties of AIEgens and the design for ASCVD management, the application of AIEgens in ASCVD biomarkers sensing, imaging and treatment of ASCVD.

Application and validation of the machine learning-based multimodal radiomics model for preoperative prediction of lateral lymph node metastasis in papillary thyroid carcinoma.

Background: Papillary thyroid carcinoma (PTC) frequently develops lateral lymph node metastasis (LLNM) in 12.6%-32.8% of patients, increasing recurrence risk and mortality.

Intravenous Thrombolysis in Acute Ischemic Stroke: A Prognostic Prediction Model and the Role of Ischemic Core Growth Rate.

Aims: The tissue window is increasingly recognized in guiding reperfusion therapy beyond the standard time window in acute ischemic stroke (AIS). This study aims to develop a nomogram incorporating an ischemic core growth rate index to provide individualized prediction of neurological outcomes in AIS patients who received intravenous thrombolysis (IVT).

Methods: A retrospective study was conducted at the First Affiliated Hospital of Soochow University (2016-2023).

Targeting ARPC1B Cancer Stem Cells to Sensitise Pancreatic Cancer to Gemcitabine Treatment.

ARPC1B cancer stem cells (CSCs) in pancreatic cancer are identified as a subpopulation resistant to gemcitabine. In our study, drug repositioning, molecular docking, and surface plasmon resonance (SPR) technique jointly revealed that CK-636 can directly target ARPC1B protein with high affinity. In vitro cytotoxicity, ex vivo organoid cultures, in vivo xenograft and orthotopic gemcitabine-resistant pancreatic cancer model demonstrated that combination therapy of gemcitabine plus CK-636 showed a superior anti-tumor effect compared with gemcitabine monotherapy.

Resident Preferences for Telemedicine Services in China in the Digital Health Era: Mixed Methods Study.

Background: In the digital health era, telemedicine has become a key driver of health care reform and innovation globally. Understanding the factors influencing residents' choices of telemedicine services is crucial for optimizing service design, enhancing user experience, and developing effective policy measures.

Objective: This study aims to explore the key factors influencing Chinese residents' choices of telemedicine services, including consultation fee, physician qualifications, appointment waiting time, scope of services, privacy protection, and service hours.

Deep Learning Application of YOLOv8 for Aortic Dissection Screening using Non-contrast Computed Tomography.

Objective: Acute aortic dissection (AD) is a life threatening condition that poses considerable challenges for timely diagnosis. Non-contrast computed tomography (CT) is frequently used to diagnose AD in certain clinical settings, but its diagnostic accuracy can vary among radiologists. This study aimed to develop and validate an interpretable YOLOv8 deep learning model based on non-contrast CT to detect AD.

Innate immune sensing of Z-nucleic acids by ZBP1-RIPK1 axis drives neuroinflammation in Alzheimer's disease.

Neuroinflammation drives Alzheimer's disease (AD) pathogenesis. Z-DNA, a non-canonical left-handed DNA structure, activates innate immune signaling through Z-DNA-binding protein 1 (ZBP1). However, the functional significance of ZBP1-mediated Z-DNA detection in AD remains undefined.