FGF9-FGFR2 Signaling via Osteocytes-Preosteoblasts Crosstalks to Mediate Mechanotransduction-Driven Intramembranous Osteogenesis in the Underdeveloped Maxilla.

Maxillary underdevelopment is a critical component of skeletal Class III malocclusion, closely linked to altered biomechanical signaling. Mechanical stimulation through early facemask protraction can effectively promote maxillary growth, yet the underlying mechanotransduction mechanisms remain unclear. In this study, fibroblast growth factor 9 (FGF9) is identified as a key biomechanical responder in maxillary development.

Single-cell RNA sequencing uncovers abnormal Sertoli-cell elevation and testicular niche impairment in the transfemales's testis.

Background: Transgender women (transfemales) often undergo gender-affirming hormone therapy (GAHT). However, the testicular impacts of feminising hormones present heterogeneity due to the complexity of testicular regulatory mechanisms.

Method: In this study, we analyzed approximately 49,385 single-cell transcriptomes from transfemale human testicular tissue, comparing cellular composition with that of cisgender male individuals across a range of ages.

Latent profiles of psychological resilience in patients with chronic disease and their association with social support and activities of daily living : Title page.

Background: This study aimed to identify different profiles of psychological resilience among patients with chronic disease and to further explore their association with demographic factors, social support, and activities of daily living (ADL).

Methods: A cross-sectional study was conducted between October and December 2023. A total of 460 patients with chronic disease were investigated by the demographic questionnaire, the Connor-Davidson Resilience Scale-10 item, the Social Support Rating Scale, and the Modified Barthel Index.

Regulation of CD4 + T cell differentiation and function by glucose metabolism.

The long-term persistence of naive T lymphocytes is maintained by a state of relative quiescence. Upon antigenic stimulation, these naive T cells undergo rapid activation and proliferation, differentiating into effector cells with specific clonal expansion. Recently, in-depth studies have revealed a fundamental difference in the metabolic requirements of distinct T cell subsets.

Effects of subjective driving workload on hazard perception performance in older drivers: A driving simulation study.

Objectives: The aim of this research was to explore the changes in eye movement characteristics, driving reaction, and operation performance of older drivers under different intersection conflict scenarios and to investigate the impact of subjective workload on hazard perception performance of older drivers.

Methods: A driving simulation experiment was conducted to simulate various intersection conflict risk scenarios, and data on driving behavior and visual characteristics were collected from 47 middle-aged and older drivers. The NASA-Task Load Index (TLX) scale was utilized to assess subjective workload across six dimensions: Mental Demands, Physical Demands, Temporal Demands, Own Performance, Effort, and Frustration.

Sintilimab and anlotinib with gemcitabine plus cisplatin in advanced biliary tract cancer: SAGC a randomized phase 2 trial.

Biliary tract cancer (BTC) has a poor prognosis with limited treatment options. This phase 2 trial randomized 80 patients with unresectable/metastatic BTC 1:1 to sintilimab, anlotinib, and gemcitabine/cisplatin (SAGC) or chemotherapy alone (GC). At 13.

Probing the Challenge of P-Type Semiconductors in Long-Chain VOC Detection: 3D Micro-Flower Zinc Cobaltate Heterojunction Sensors.

Tetradecane, a long-chain alkane recently recognized as a volatile marker for early-stage mildew detection in stored grains and as a reference material in hydrocarbon studies, presents significant challenges for detection due to its inherent low reactivity and substantial molecular size. This study reports the synthesis of a microflower-like Fe@WO/ ZnCoO heterostructure for effective tetradecane sensing. The Fe@WO/ ZnCoO n-p junctions demonstrated significant alterations in electrical conductivity upon exposure to tetradecane at room temperature.

Effect of Nano-SiO on the Hydration, Microstructure, and Mechanical Performances of Solid Waste-Based Cementitious Materials.

Solid waste-based cementitious materials (SWBC) are composed of steel slag (SS), granulated blast furnace slag (GBFS), fly ash (FA), desulfurization gypsum (DG), and Portland cement (PC). Currently, SWBC holds great potential as a sustainable building material; however, its low early compressive strength and volume expansion limit its range of application. Therefore, the main objective of this study is to enhance the mechanical properties and dimensional stability of SWBC by adding nano-SiO, while also improving its resistance to chloride ions, thereby promoting its use in the field of sustainable building materials.

An integrated framework for universal coagulation test reference intervals: Cross-platform validation of eleven indirect algorithms and age-specific hierarchical optimization.

Background: The conventional direct method for establishing coagulation reference intervals (RIs) faces challenges, including difficulties in recruiting healthy populations, non-Gaussian distributions, and complex age-related effects. Indirect methods utilizing real-world data (RWD) are limited by a lack of consensus on algorithm selection and insufficient cross-platform validation.

Objective: To develop a multi-algorithm collaborative framework for systematically evaluating the performance of 11 outlier detection algorithms, thereby optimizing coagulation RIs for the Chinese population and validating their cross-platform applicability.

Impaired glucagon suppression accompanied by liver fat accumulation mediated worse blood glucose control in T2D patients.

Background: Metabolic dysfunction-associated steatotic liver disease is prevalent in type 2 diabetes (T2D) and exacerbates hyperglycemia, but its impact on postprandial glucagon suppression remains unclear.

Objective: To investigate the association between hepatic steatosis and impaired glucagon suppression during oral glucose tolerance tests (OGTT), and to evaluate the mediating role of glucagon dysregulation in linking liver fat to glycemic control.

Design And Methods: In this cross-sectional study, 604 patients with T2D underwent liver fat quantification via FibroScan Pro®-controlled attenuation parameter (CAP) and liver ultrasound.

Atopic dermatitis relapse after treatment discontinuation and predictive factors for relapse: JAK1 inhibitors versus dupilumab.

Background And Objectives: In the clinical management of atopic dermatitis (AD), treatment discontinuations and subsequent disease relapse are common. Understanding relapse patterns following treatment discontinuation in AD patients is therefore essential. This study aims to investigate the time to relapse and identify predictive factors in patients with moderate-to-severe AD who responded to treatment with selective Janus kinase 1 (JAK1) inhibitors (upadacitinib or abrocitinib) or dupilumab.

Suppressor of cytokine signaling 2 modulates regulatory T cell activity to suppress liver hepatocellular carcinoma growth and metastasis.

Background: Liver hepatocellular carcinoma (LIHC) is a highly aggressive cancer with poor prognosis due to its complex tumor microenvironment (TME) and immune evasion. Regulatory T cells (Tregs) play a critical role in tumor progression. Suppressor of cytokine signaling 2 (SOCS2), a key immune regulator, may modulate Treg activity and impact LIHC growth and metastasis.

Single-Molecule Liquid Biopsy Detects Low- and High-Abundance Protein Markers Simultaneously for Pancreatic Cancer Diagnosis.

Simultaneous analysis of multiple biomarkers can typically improve the sensitivity and specificity of a disease diagnosis. Low-abundance serum proteins have recently emerged as a novel class of biomarkers for diseases. Due to the low concentration, the low-abundance protein analysis relies on single-molecule immunoassay, which has a very limited dynamic range.

Actively NO-Reducing Oxygen-Tolerant Microbial Consortium Attained by Using a High-Dilution-Rate Chemostat Fed with Methanol.

Nitrous oxide-reducing bacteria (NORB) are generally considered the only biological sink for the potent greenhouse gas NO. Although NO consumption activities by diverse heterotrophic NORB have been detected, knowledge gaps remain about the phylogenies, physiologies, and activities of NORB. Here, we successfully enriched a methylotrophic NORB consortium under intermittent oxygen and NO supplies.

Alterations of gut microbiota for the onset and treatment of psoriasis: A systematic review.

Psoriasis is a chronic, recurrent and systemic inflammatory skin disease which is mediated by immunoreaction. Its pathogenesis is multifactorial, and the exact driving factor remains unclear. Recent studies showed that gut microbiota, which maintain immune homeostasis of our bodies, is closely related with occurrence, development and prognosis of psoriasis.

Single cell protein production potential of enriched microbial populations from rice paddy soils and roots: Insights into protein yield enhancement by Methylophilaceae.

This study aimed to determine key factors for enriching methylotrophic populations for single-cell protein (SCP) production and to determine the predominant active methylotrophs. Microbial populations from rice paddy soils and roots, hotspots for CH oxidation, underwent serial incubation by feeding CH and O. The protein content was primarily influenced by the incubation stage, with a lesser effect from the rice plant roots as an inoculum source, reaching a maximum of 20 % in biomass dominated by Methylomonadaceae and Methylophilaceae.

Challenges in AI-driven Biomedical Multimodal Data Fusion and Analysis.

The rapid development of biological and medical examination methods has vastly expanded personal biomedical information, including molecular, cellular, image, and electronic health record datasets. Integrating this wealth of information enables precise disease diagnosis, biomarker identification, and treatment design in clinical settings. Artificial intelligence (AI) techniques, particularly deep learning models, have been extensively employed in biomedical applications, demonstrating increased precision, efficiency, and generalization.

The effect and mechanism of microplastics to the NO emission in underground and aboveground wastewater treatment plants.

This study investigated the effect and microbial mechanism of microplastics to the NO emission in underground and aboveground wastewater treatment plants. The microplastics in the influent of Uwwtp and Awwtp were 4953 ± 558 and 2253 ± 563 particles/L. The microplastics rejection rate were 86.

Risk factors for stoma and incision complications of enterostomy in children with very early-onset inflammatory bowel disease: a prospective cohort study.

Unlabelled: Enterostomy is utilized to mitigate severe clinical symptoms in children with very early-onset inflammatory bowel disease (VEO-IBD) and to provide a window for stem cell transplantation. Nevertheless, the incidence of postoperative complications is significant, and there is currently a lack of research exploring the risk factors associated with complications related to the stoma and incision following the procedure. The objective of this study is to investigate the risk factors for stoma and incision complications after enterostomy in patients with VEO-IBD.

All-Optical Single-Channel Plasmonic Logic Gates.

Optical computing, renowned for its light-speed processing and low power consumption, typically relies on the coherent control of two light sources. However, there are challenges in stabilizing and maintaining high optical spatiotemporal coherence, especially for large-scale computing systems. The coherence requires rigorous feedback circuits and numerous phase shifters, introducing system instability and complexity.

The evaluation of targeted exome sequencing of candidate genes in a Han Chinese population with primary open-angle glaucoma.

Primary open-angle glaucoma (POAG), known as a common ocular disease with genetic heterogeneity, is characterized by progressive optic disc atrophy and visual field defects. This study aimed to assess the contribution of previously reported POAG-associated genes and investigate potential functional variations and genotype-phenotype correlations in a Han Chinese population. DNA from 500 cases and 500 controls was pooled and sequenced using a customized panel of 398 candidate genes.

Clinical characteristics and ultrasound biomicroscopic evaluation of anterior segment dysgenesis: a retrospective cross-sectional study.

Background: Anterior segment dysgenesis (ASD) encompasses a range of congenital disorders affecting the development of the eye's anterior segment, often leading to significant visual impairment and glaucoma. Although numerous studies have focused on the genetic basis of ASD, few have comprehensively compared the clinical features across ASD subtypes. This study aims to address this gap by describing and comparing the clinical characteristics and ultrasound biomicroscopy (UBM) imaging features of various ASD subtypes in a Chinese tertiary medical center.