Characteristics of CVA16 B1c strains isolated for the first time in the Heilongjiang Province of China.

Hand, foot, and mouth disease (HFMD) represents a globally prevalent infectious disease that is caused by enteroviruses. Enterovirus A71 (EV-A71), coxsackievirus A16 (CVA16), and coxsackievirus A6 (CVA6) are recognized as the predominant causative agents of HFMD. CVA16 is a member of the genus within the family .

The First Imported Case of Zika Virus Infection - Shandong Province, China, 2025.

What Is Already Known About This Topic?: Zika virus (ZIKV) is transmitted primarily through mosquito vectors, including and , both of which are distributed across multiple provinces in China. Approximately 80% of ZIKV infections remain asymptomatic, while symptomatic cases typically manifest as mild, self-limiting illnesses lacking pathognomonic features. Common clinical presentations include maculopapular rash, low-grade fever, conjunctivitis, arthralgia, and myalgia.

Foodborne Botulism Caused by Subtype A5(b3) by Self-Packaged Vacuum Spicy Rabbit Heads.

Botulism is a severe muscle paralysis disease mediated by the botulinum toxin. Here, we reported a foodborne botulism case caused by subtype A5(b3) from self-packaged vacuum spicy rabbit heads. Treatment for this case was delayed due to misdiagnosis and insufficient diagnostic capacity in three hospitals, which resulted in progressive clinical deterioration, and eventually, the patient was transferred to Shandong Public Health Clinical Center for specialized therapy.

Global Evolution and Transmission Dynamics of Enterovirus D68.

Enterovirus D68 (EV-D68), a serotype of the enterovirus species D, has garnered significant attention due to outbreaks reported in 2014, 2016, and 2018. In this study, 36 Chinese EV-D68 strains were isolated, sequenced, and combined with all EV-D68 VP1 sequences from GenBank to form a data set of 1679 sequences. This data set served as the basis for phylogenetic, evolutionary dynamics, phylogeographic, and key amino acid site mutation analyses of EV-D68.

Isolation and identification of Alcaligenes faecalis W2-3 with high-yield production of dimethyl disulfide.

Dimethyl disulfide (DMDS) is an important volatile organic sulfur compound with diverse applications and substantial demand in the chemical, agricultural, and food industries. The challenges associated with their chemical synthesis have spurred exploration of biological synthesis as an alternative route. Nevertheless, there is limited research on the biosynthesis of DMDS, and the yield remains low.

Coverage of HPV Vaccination and Influencing Factors Among Female College Students in Northern China.

Despite the significant global disease burden associated with HPV infection, the vaccination coverage among female college students in China remains suboptimal. This study aimed to examine HPV vaccination coverage, knowledge levels, and determinants influencing vaccination behavior among female college students in northern China, utilizing the Health Belief Model (HBM) as a theoretical framework. A cross-sectional online survey was conducted from December 2024 to January 2025, involving 4076 female students from six universities in Jinan, China.

Lactate metabolism-related genes serve as potential biomarkers for predicting gastric cancer progression and immunotherapy.

Background: Targeting lactate metabolism represents a promising therapeutic strategy to enhance anti-tumor immune responses. In this study, we developed a novel model based on lactate metabolism-related genes (LRGs) to predict survival, characterize the immune microenvironment, and assess immunotherapy response in gastric cancer (GC), with the potential to identify new biomarkers.

Methods: Data sets of GC patients were obtained from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases.

Effects of social support on cognitive frailty among the older adults in China: mediation of psychological resilience and moderated mediation of education.

Objectives: Previous research has revealed a relationship between social support and cognitive frailty. However, the underlying mechanisms of this connection have still not been well explored. The study aimed to investigate the effect of social support on cognitive frailty, mediated by psychological resilience and to examine the moderated mediation effect of education.

Factors associated with acceptance of preventive treatment among college students with latent tuberculosis infection in Shandong, China.

Objectives: In China, little is known about factors associated with acceptance of tuberculosis (TB) preventive treatment (TPT) among college students. This study investigates the factors associated with the acceptance of TPT for latent tuberculosis infection (LTBI) among college students.

Study Design: This was a cross-sectional study.

Predictors of Tuberculosis Preventive Treatment Completion in College Students: A High Adherence Study in China.

Purpose: To investigate and evaluate factors associated with completion of tuberculosis preventive treatment (TPT) among college students with latent tuberculosis infection (LTBI).

Patients And Methods: We conducted a cross-sectional survey of 399 LTBI college students who initiated TPT in Shandong Province, China. TPT completion rates were determined.

Childhood Smoking Initiation, Genetic Susceptibility, and Incident Cardiovascular Diseases in Adulthood.

Purpose: We aimed to assess the associations of age of smoking initiation and polygenic risk scores (PRSs), individually and jointly, with the incidence of overall cardiovascular diseases (CVDs), coronary artery disease (CAD), atrial fibrillation (AF), and stroke in adulthood.

Methods: Multivariable-adjusted accelerated failure time models were used to assess the impacts of age of smoking initiation and PRSs on incident overall CVDs, CAD, AF, and stroke in adulthood and to examine their joint effect on these outcomes.

Results: Compared with never smokers, the time ratio (TR) with a 95% confidence interval (CI) for incident overall CVDs was 0.

[The spatio-temporal trend of female breast cancer incidence and mortality in Shandong Province from 2012 to 2023 and trend prediction].

We aimed to analyze the spatio-temporal trend of breast cancer incidence and mortality in Shandong Province from 2012 to 2023 and predict the development trend from 2024 to 2030. Data on the incidence and mortality of breast cancer in Shandong Province from 2012 to 2023 were obtained from the Shandong Cancer Registry. The incidence, age-specific incidence, mortality, and age-specific mortality in different years, as well as in urban and rural areas, were calculated, and the rates were standardized based on the age composition of the Chinese standard population in 2000.

The impact of leisure sedentary behaviors on risk of chronic kidney disease, diabetes, and related complications: Mendelian randomization study.

Background: The causal relationship between leisure sedentary behaviors (LSBs) and chronic kidney disease, diabetes and related complications is still equivocal. In this study, we performed two-sample Mendelian randomization for declaring the potential causal association between LSBs and these diseases and summarized the causal estimates.

Methods: In this study, we used GWAS summary statistics from the public database for exposures (LSB: television watching, computer use, and driving) and outcomes (chronic kidney diseases, diabetes mellitus, and related complications).

Mechanisms of Antimicrobial Resistance in : Advances in Detection Methods and Clinical Implications.

. are ubiquitous gram-negative bacteria, commonly present in natural environments and as part of the human microbiota. is involved in the occurrence and development of many diseases, and effective antibiotics for it have attracted the attention of researchers.

A vacuum lyophilization and bacterial tablet-based method for culture medium evaluation and potential use in probiotic products.

Introduction: The fundamental step in microbiological detection is the preparation of culture medium. The reliability and accuracy of microbiological assay heavily relies on this step. Currently in China, the most recognized standard method for such medium evaluation is ISO 11133-2014.

Phylogenetic and Genetic Evolution Analysis of Complete SFTSV Genome Sequences in Shandong Province, China.

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by SFTS virus (SFTSV). Shandong province is one of the epidemic regions with high incidence rate of SFTS. To investigate phylogenetical and genetic evolution characteristics of SFTSV in Shandong province, we isolated SFTSV from suspected patients between April 2023 and October 2024, and then whole SFTSV genomes were amplified and sequenced in this study.

Long-term predictions of COVID-19 waves in China based on an improved SEIRS-Q model of antibody failure.

Introduction: China had already experienced two COVID-19 epidemics since the promulgation of 10 new prevention and control measures in December 2022.

Methodology: In response to the current frequent epidemics of severe acute respiratory syndrome coronavirus 2 variants in China and the gradual relaxation of prevention and control policies, we built and ran a susceptible-exposed-infective-removed-susceptible-quarantined model incorporating self-isolation to predict future cases of COVID-19.

Results: Four waves of outbreaks were predicted to occur in November 2023, and in April, July, and November 2024.

TiltRec: an ultra-fast and open-source toolkit for cryo-electron tomographic reconstruction.

Motivation: Cryo-electron tomography (cryo-ET) has revolutionized our ability to observe structures from the subcellular to the atomic level in their native states. Achieving high-resolution reconstruction involves collecting tilt series at different angles and subsequently backprojecting them into 3D space or iteratively reconstructing them to build a 3D volume of the specimen. However, the intricate computational demands of tomographic reconstruction pose significant challenges, requiring extensive calculation times that hinder efficiency, especially with large and complex datasets.

Epidemiological Features and Evolutionary Analysis of Recombinant CVA24v in the Context of a Re-Emerging AHC Outbreak in China, 2023.

China experienced another outbreak of acute hemorrhagic conjunctivitis (AHC) in 2023, with a total of 195 297 recorded cases. This marks the third nationwide outbreak in nearly two decades, following previous outbreaks in 2007 and 2010. Descriptive epidemiological analysis shows that the number of cases in 2023 peaked in September, similar to the last two outbreaks.

The molecular receptor NKBB enhances the persistence and anti-hepatocellular carcinoma activity of GPC3 CAR-T cells.

Chimeric antigen receptor (CAR) T cells have encouraging results in the treatment of hematological malignancies. However, CAR-T therapy still faces numerous challenges against solid tumors, such as hepatocellular carcinoma (HCC), owing to heterogeneous antigen expression in tumor cells, limited persistence of CAR-T cells, etc. Therefore, to treat HCC more effectively, we connected the molecular receptor NKBB to a second-generation glypican-3 (GPC3) CAR to construct GC3328z-NKBB CAR-T cells, which have double specific targets of GPC3 and NKG2DLs (natural killer group 2, member D ligands), dual co-stimulation of CD28 and 41BB, and a single CD3ζ chain.

3D Printed Titanium Scaffolds with Bi-Directional Gradient QK-Functionalized Surface.

3D printed titanium scaffold has promising applications in orthopedics. However, the bioinert titanium presents challenges for promoting vascularization and tissue growth within the porous scaffold for stable osteointegration. In this study, a modular porous titanium scaffold is created using 3D printing and a gradient-surface strategy to immobilize QK peptide on the surface with a bi-directional gradient distribution.

Transcriptome Profiles Characteristics of the Peripheral Immune in Patients with Severe Fever with Thrombocytopenia Syndrome.

Purpose: Severe fever with thrombocytopenia syndrome (SFTS) is an acute viral infection disease with a high mortality, but there are no specific effective drugs or vaccines available for use. To develop effective treatment methods, more basic researches are urgently needed to elucidate the response mechanisms of patients.

Patients And Methods: Here, we conducted the transcriptomic analysis of peripheral immunity in 14 SFTS patients, ranging from moderate infection to severe and fatal disease.