Enhancing dry-fermented sausages: Meyerozyma guilliermondii as a flavor-boosting yeast in co-inoculation with Latilactobacillus sakei.

Background: Dry-fermented sausages are traditional meat products, and their quality and flavor largely depend on the complex microbial interactions during the fermentation process. Lactic acid bacteria (LAB) are widely used in the fermentation of sausages, but studies have shown that yeast has potential in terms of flavor complexity and process performance. Therefore, exploring the co-inoculation of yeast and LAB can provide new opportunities for optimizing the production of fermented sausages.

Surface-engineered nanoprobes for multimodal bioimaging: from molecular design to theranostic integration.

Bioimaging technologies are indispensable for deciphering cellular dynamics, disease mechanisms, and therapeutic responses. However, conventional agents are limited by photobleaching, poor tissue penetration, and nonspecific distribution. These shortcomings hinder real-time visualization of deep tissues and complex pathologies.

Innovative applications of plasma-activated water in Chinese mitten crab (Eriocheir sinensis): Improving quality, safety and sustainability.

This study explores plasma-activated water (PAW) as a sustainable aquaculture water treatment for Chinese mitten crab (Eriocheir sinensis), evaluating its impact on antioxidant responses, microbial dynamics, and crab quality. We evaluated the physiological and microbial responses of E. sinensis using high-throughput sequencing and antioxidant enzyme assays.

Method for screening near-infrared quantitative models with high robustness.

Background: It is a common issue that the prediction results of near-infrared (NIR) calibration models are sensitive to changes in measurement conditions. Many existing chemometric algorithms focus on optimizing the accuracy of NIR quantitative models. However, to ensure that a NIR model remains effective across a broader range of working conditions and minimizes the need for recalibration, a more robust model-though not necessarily the most accurate one-may sometimes be a more suitable choice.

Mechanism of reducing rice starch digestibility by quinoa protein amyloid-like fibrils pretreated with magnetic field.

The study aimed to investigate the mechanism by which magnetic field-pretreated quinoa protein amyloid-like fibrils affected the digestive properties of rice starch. Magnetic field pretreatment induced conformational changes in quinoa protein, promoting fibrillation, and quinoa protein formed the most amyloid-like fibrils under a 3 mT magnetic field treatment. The higher amyloid-like fibrils content enhanced both the short-range and long-range ordered structures of rice starch, as evidenced by the increased R value (from 0.

Rapid determination of biogenic amines in meats using quinone chemistry-based colorimetric strategy.

Rapid and convenient detection of biogenic amines (BAs) in meat products is a vital approach for ensuring food quality and consumer health. In this study, we developed a quinone chemistry-mediated colorimetric strategy for the rapid detection of BAs in meat. BAs such as putrescine undergo a visible colorimetric reaction with 2-methyl-1,4-benzoquinone (MBQ), producing a product with strong UV-visible absorption at 480 nm, enabling specific colorimetric detection of BAs.

Unraveling the flavor formation mechanisms of Wanxi dry-cured goose: Microbiomics, metabolomics, and flavoromics study.

Wanxi dry-cured goose (WXDG) is a traditional Chinese preserved meat product known for its distinctive flavor. However, the underlying mechanisms of its flavor formation remain unclear. This study aimed to explore the potential mechanisms driving the flavor formation by utilizing a multi-omics approach, including microbiomics, metabolomics, and flavoromics.

Porphyrin-Decorated Sodium Alginate Hydrogel with Carbon Nanotubes for Confinement-Enhanced Electrochemiluminescence and Antibiotic Resistance Gene Detection.

Gel-type electrochemiluminescence (ECL) active materials have attracted attention in sensing applications, where solid-state flexible and robust-response gels are required. Herein, we report a 5,10,15,20-tetrakis(4-sulfonatophenyl) porphyrin-decorated sodium alginate hydrogel (TPPS-SA hydrogel) as a new-generation ECL cathode luminophore. TPPS-SA hydrogel was synthesized by a facile hydrogen bond interaction.

Design and Semisynthesis of Ubiquitin Extension Probes for Structural Analysis of Cullin1-Mediated Substrate Polyubiquitination.

Chemical trapping strategies have recently emerged as powerful approaches for investigating the structural dynamics of E3 ligase-catalyzed substrate ubiquitination. However, current ubiquitination-derived probes are limited to studying substrate mono- or diubiquitination events. Probes capable of investigating how E3 ligases accommodate E2-Ub conjugates and ubiquitinated substrates to generate longer ubiquitin chains remain unexplored.

High-entropy nanozyme-driven "Chinese acupuncture" hydrogel microneedles for combined therapy of infected scalds.

Bacterial infections, especially those caused by antibiotic-resistant (), hinder the healing of scalds in food processing environments. This study developed a high-entropy nanozyme (HEzyme) hydrogel system that exhibited efficient reactive oxygen species (ROS) and photothermal antibacterial effects. The HEzyme was synthesized using a carbon-oxygen-silicon framework-assisted method, incorporating multiple metal species (CoNiCuZnCe).

A Woundplast of Hydrogel Microneedles with ATP-Responsive Therapeutic Logic for Bacteria-Infected Wound Healing.

To reduce the overuse of antibiotics, on-demand antibiotic administration guided by rapid infection assessment is of particular importance. In this study, we present a portable, multifunctional hydrogel microneedle (MN) woundplast capable of real-time monitoring of wound adenosine triphosphate (ATP) levels and simultaneous antibacterial therapy. By leveraging the aggregation-induced emission effect, metal-organic frameworks (MOFs) coencapsulating gold nanoclusters (AuNCs) and vancomycin are integrated into the MN tips, where ATP-Zn interactions enable selective ATP recognition and quantification across 0-5 mM.

Structural characterization, interaction mechanism and thermal stability of dual-fibrous complexes formed by rice glutelin fibrils and cellulose nanocrystals.

Food protein fibrils and polysaccharides have the potential to form novel complexes through non-covalent interactions. In this study, we prepared enzymatically pretreated rice glutelin fibrils (RGF)-cellulose nanocrystals (CNC) complexes and explored their interaction mechanism. The results showed that the turbidity and particle size increased with the increase in CNC ratio, which indicated that RGF and CNC were successfully combined.

The effect of uracil on the freeze-drying survival rate of Lactiplantibacillus plantarum YR07 based on transcriptome analysis.

This study aimed to elucidate how adding uracil to the culture medium enhanced the freeze-drying resistance of Lactiplantibacillus plantarum YR07. The results showed that uracil significantly increased the number of viable cells and the freeze-drying survival rate of L. plantarum YR07 (P < 0.

Epigenetic reprogramming of HDAC2 in CA1 excitatory neurons determines Pb-induced non-spatial memory deficits.

There is robust evidence that environmental risk factors impact cognitive performance. However, the effect of the environmental risk factor lead (Pb) on susceptibility to non-spatial memory deficits is unknown. Here, it is demonstrated that Pb exposure increases the susceptibility to these deficits, with the impairment of CA1 excitatory neurons as the underlying cause.

Enhancing Indica Rice Wine Quality by Adding Acid Protease and Lactobacillus Plantarum F25-4 During Soaking Process.

Indica rice is affordable and readily available, making it an excellent raw material for brewing indica rice wine (IRW). However, indica rice contains high amylose and protein content, which often results in suboptimal alcohol yield and flavor profiles. This study developed a novel synergistic soaking process combining acid protease with Lactobacillus plantarum F25-4 to address these limitations.

Difference in mechanism of gelatinization and transparency among chicken, pigeon and turtle egg white thermogels.

This study aimed to investigate the essential reasons for difference in gel and optical properties of chicken (CEW), pigeon (PEW) and turtle (TEW) egg white (EW) thermogels (TGs) and explore the underlying mechanism governing from their major protein. The variation in gelatinization and transparency of CEW, PEW and TEW mainly depends on the type and concentration of host proteins. TG cannot (or hardly) be obtained in TEW primarily rely on higher levels of free water (97.

Advancements in Intelligent Sensing Technologies for Food Safety Detection.

As a critical global public health concern, food safety has prompted substantial strategic advancements in detection technologies to safeguard human health. Integrated intelligent sensing systems, incorporating advanced information perception and computational intelligence, have emerged as rapid, user-friendly, and cost-effective solutions through the synergy of multisource sensors and smart computing. This review systematically examines the fundamental principles of intelligent sensing technologies, including optical, electrochemical, machine olfaction, and machine gustatory systems, along with their practical applications in detecting microbial, chemical, and physical hazards in food products.

Whey protein-constructed oleogels as cheese fat replacers: Modulating cheese functional behavior via protein structure modification.

Whey protein aggregates (WPA), formed through controlled thermal denaturation and aggregation, have emerged as novel oleogelators with exceptional functional properties. This study aims to investigate the relationship between WPA's structure and their oleogelation performance in sunflower oil, while evaluating their potential as fat replacers in cheese formulations. By modulating heating condition (85-95 °C/15 min) and protein concentrations (5-20 %), distinct WPA microstructures were engineered.

Differences in DHA bioaccessibility and fat digestion of DHA-enriched milks: An in vitro infant digestion model.

Docosahexaenoic acid (DHA) is essential for infant neurodevelopment, and milk is an effective vehicle for delivering DHA to infants. However, it remains unclear how different production and processing methods affect the bioavailability of milk study evaluated the bioaccessibility of DHA in different DHA-enriched milks using a simulated infant digestive model. The results showed that naturally DHA-enriched milk and homogenized naturally DHA-enriched milk exhibited higher DHA bioaccessibility (52.

Synergistic effects of alternating magnetic field and sodium tripolyphosphate on functional properties of myofibrillar proteins in low-salt systems.

This study investigated the synergistic regulatory mechanisms of alternating magnetic field (AMF) and sodium tripolyphosphate (STPP) on the functional properties of myofibrillar protein (MP) in a low-salt environment. It was found that AMF promotes an increase in MP solubility. When the magnetic field strength was >6 mT, the formation of hydrophobic interactions within the MP molecules reached a dynamic equilibrium with the action of the AMF, resulting in a slowing down of the increase in MP solubility and emulsification activity.

Carboxymethyl-β-cyclodextrin grafted chitosan embedded oyster (Crassostrea gigas) ferritin can alleviate lead-induced liver injury by oral administration.

Ferritin is expected to alleviate lead-induced oxidative damage. Despite being a relatively stable macromolecular protein that can withstand high temperatures and extreme pH conditions without its structure and activity being compromised, oral administration of ferritin may still result in its degradation within the gastrointestinal tract, reducing its bioavailability. In recent years, microcapsules have emerged as an effective approach in oral protein delivery to enhance bioavailability.

Deoxynivalenol induces pyroptosis and IL-1β secretion via P2X7R signal in murine RAW264.7 macrophages.

Deoxynivalenol (DON), a trichothecene mycotoxin, exerts pro-inflammatory and immunomodulatory activity. Interleukin (IL)-1β serves a crucial part as a gate keeper of inflammation in DON-induced macrophages, but an overview of how DON exposure elicits IL-1β secretion from RAW264.7 cells has not been fully illustrated.

One-pot RPA-CRISPR/Cas12a integrated dual-mode electrochemical lateral flow strip for ultrasensitive and precise detection of Salmonella.

Rapid and accurate screening of pathogenic contamination is essential for timely intervention and infection prevention. In this work, one-pot RPA-CRISPR/Cas12a strategy combined with an innovative electrochemical lateral flow strip (OPRCC-eLFS) was presented for ultrasensitive and precise detection of Salmonella. Highly sensitive dual-mode detection of Salmonella in various samples has been simultaneously achieved with electrochemical detection limit of 3.

Enhanced lipid production in Chlamydomonas reinhardtii: Impact of salt stress synergized with α-lipoic acid.

The Chlamydomonas reinhardtii strain is a widely used model for studying algal lipid metabolism. This research investigated the effects of exogenous α-lipoic acid (ALA) on growth and lipid accumulation under salt stress. After 7-day ALA treatment, the biomass (0.

SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.

Introduction: Sialic acid (SA) is an essential nutrient for brain development and cognition. Infants lack the capacity to synthesize sufficient SA independently, requiring reliance on maternal or exogenous sources. For early nutritional supplementation, elucidating how SA affects learning and memory is necessary.