Large-scale genomic deletion in activates immune responses and confers resistance to rice bacterial blight.

Lesion mimic mutants (LMMs) are invaluable for uncovering the molecular mechanisms of programmed cell death (PCD) and plant immunity and identifying more LMMs expands our understanding of these complex processes. In this study, we characterized a novel rice LMM, , identified through heavy-ion beam irradiation. The mutant exhibits reddish-brown lesions from the tillering stage, reduced plant height, grain size, and fertility.

Accurate quantitative detection of sodium (Na) content in sorghum roots based on multi-source data fusion of LIBS and HSI.

The study of sodium content in plants is crucial for the improvement of saline-alkali soil. Existing metal element detection methods pose challenges because they are complicated and time-consuming. In this study, we propose a quantitative detection model, FusionNet, that integrates Laser-Induced Breakdown Spectroscopy (LIBS) and Near-Infrared Hyperspectral Imaging (NIR-HSI) to realize the detection of Na element content in sorghum roots.

Prevalence and genomic insights into Yersinia enterocolitica in Southeastern China (2008-2022).

Yersinia enterocolitica is a significant foodborne pathogen causing gastrointestinal illnesses worldwide. This study investigates the prevalence and genomic characteristics of Y. enterocolitica to assess potential health risks in southeastern China, a region lacking mandatory yersiniosis monitoring.

Method for screening near-infrared quantitative models with high robustness.

Background: It is a common issue that the prediction results of near-infrared (NIR) calibration models are sensitive to changes in measurement conditions. Many existing chemometric algorithms focus on optimizing the accuracy of NIR quantitative models. However, to ensure that a NIR model remains effective across a broader range of working conditions and minimizes the need for recalibration, a more robust model-though not necessarily the most accurate one-may sometimes be a more suitable choice.

Chlamydomonas reinhardtii (Red) Rich in Protoporphyrin IX Exerts Anti-Diabetic Effects in Liver Tissue.

The metabolites derived from microalgae have been attributed with various nutritional and medicinal properties. Therefore, our research focused on exploring the potential benefits of Chlamydomonas reinhardtii (red) for type 2 diabetes mellitus (T2DM) patients. Mice were subjected to a high-fat diet and low-dose streptozotocin injection to develop T2DM.

Effects of group reminiscence therapy assisted by memory specificity training (GRT-mest) on depressive symptoms in older adults: A cluster randomized controlled trial.

Objectives: To investigate the effects of the Group Reminiscence Therapy assisted by Memory Specificity Training (GRT-mest) in older adults, and to further investigate the indirect effect of autobiographical memory in improving depressive symptoms.

Methods: In this single-blind, three-arm parallel group, cluster randomized controlled trial, 78 eligible participants were randomly assigned to two intervention groups and one control group in two nursing homes, and assessments were conducted at baseline, immediately post-intervention, and 1-month and 3-month follow-ups. Data analysis was performed using SPSS 26.

Development and validation of a HPLC-MS/MS method the determination of genistein and equol in serum, urine and follicular fluid.

Soy isoflavones exert estrogen-like synergistic or antagonistic effects by binding to estrogen receptors, and potentially impact the function of female reproductive system, but their distribution profile in human remains little clarified. To determination of genistein (GEN) and equol (EQ) in human urine, serum and follicular fluid (FF), an analytical method based on high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed and validated. The enrichment and clean-up are performed on a solid-phase extraction (SPE) column; the elution is a gradient one, with the mobile phase (A) of 0.

Toxicological evaluation of Chlamydomonas reinhardtii enriched with protoporphyrin IX.

The Chlamydomonas reinhardtii (red) is a strain rich in protoporphyrin IX, which could be used as algal-derived novel sustainable nutritional sources. However, the safety research on C. reinhardtii (red) remains insufficient and further investigation is needed.

HCN4 channels sense temperature and determine heart rate responses to heat.

The hyperpolarization-activated cyclic nucleotide-gated ion channel 4 (HCN4) current increases due to cAMP binding and is well-recognized to contribute to adrenergically driven heart rate acceleration. HCN4 current also increases with heat by an unknown mechanism(s). We use thermodynamical and homology computational modeling, site-directed mutagenesis, and mouse models to identify a concise motif on the S4-S5 linker of HCN4 channels (M407/Y409) that determines HCN4 current (I) responses to heat.

Effectiveness of Mobile Health-Based Gamification Interventions for Improving Physical Activity in Individuals With Cardiovascular Diseases: Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Background: Gamification refers to using game design elements in nongame contexts. Promoting physical activity (PA) through gamification is a novel and promising avenue for improving lifestyles and mitigating the advancement of cardiovascular diseases (CVDs). However, evidence of its effectiveness remains mixed.

Infection Status, Etiological Analysis of spp. in Foodborne Diarrhea Patients from 2019 to 2023 in Wenzhou.

The infection status and etiological analysis of spp. from foodborne diarrhea patients in Wenzhou were carried out to provide the etiological basis for healthy diet and clinical treatment. isolates ( = 41) collected from foodborne diarrhea patients were identified using the automatic bacteriologic analyzer and mass spectrometer.

Mutation of rice EARLY LEAF LESION AND SENESCENCE 1 (ELS1), which encodes an anthranilate synthase α-subunit, induces ROS accumulation and cell death through activating the tryptophan synthesis pathway in rice.

Lesion-mimic mutants (LMMs) serve as valuable resources for uncovering the molecular mechanisms that govern programmed cell death (PCD) in plants. Despite extensive research, the regulatory mechanisms of PCD and lesion formation in various LMMs remain to be fully elucidated. In this study, we identified a rice LMM named early leaf lesion and senescence 1 (els1), cloned the causal gene through map-based cloning, and confirmed its function through complementation.

Mutation of GLR2 confers enhanced glufosinate resistance and salt tolerance in rice.

The GLR2 transcription factor regulates glufosinate resistance and enhances abiotic stress tolerance in rice through interaction with the GLR1 transcription factor and modulation of gene expression.

A novel variable selection algorithm based on neural network for near-infrared spectral modeling.

Background: Partial least squares (PLS) is a widely used technique for modeling spectral data. Researchers have developed numerous PLS-based variable selection algorithms to enhance model predictive ability and interpretability. In recent years, as neural network technology has advanced, these algorithms have been increasingly applied to spectral data modeling.

Mutation of rice SM1 enhances solid leaf midrib formation and increases methane emissions.

The leaf midrib system is essential for plant growth and development, facilitating nutrient transport, providing structural support, enabling gas exchange, and enhancing resilience to environmental stresses. However, the molecular mechanism regulating leaf midrib development is still unclear.In this study, we reported a rice solid midrib 1 (sm1) mutant, exhibiting solid leaf aerenchyma and abaxial rolling leaves due to abnormal development of parenchyma and bulliform cells.

Mutation of KAP, which encodes a keratin-associated protein, affects grain size and yield production in rice.

Grain size and shape are critical agronomic traits that directly impact rice grain yield. Identifying genes that control these traits can provide new strategies for yield improvement. In this study, we characterized a rice mutant, reduced grain length (rgl), which exhibited decreased grain length due to reduced cell proliferation.

Epidemiological and molecular investigations of sequential outbreaks of Campylobacter jejuni infecting adults and schoolchildren in southeastern China, 2021-2022.

Objectives: To investigate cases of five Campylobacter jejuni outbreaks and describe laboratory characteristics of these infections.

Methods: Whole-genome sequencing and conventional methods were combined to thoroughly investigate the outbreaks, and data of contemporaneous sporadic cases was included for comparison.

Results: Seven sequence types (STs) of C.

An effective moisture interference correction method for maize powder NIR spectra analysis.

The detection of maize starch content is of great significance for maize processing industry and near-infrared spectroscopy (NIRS) is an ideal rapid detection technology. However, the interference of moisture in maize is a bottleneck problem that affects the accuracy of NIRS quantitative analysis. In this study, we proposed methods based on external parameter orthogonalization (EPO) combined with wavelength selection algorithms to bring more accurate analytical results.

Effects of Laughter Therapy on Improving Negative Emotions Associated with Cancer: A Systematic Review and Meta-Analysis.

Introduction: With aging and growth of the population, the risk of cancer incidence and mortality is rapidly increasing. However, psychosocial treatment has been seriously neglected in many healthcare settings. Laughter therapy is a therapeutic program to improve emotional wellbeing and health which has been applied as a complementary treatment.

The cross-cultural adaptation and psychometric properties of the Graded Chronic Pain Scale-Revised-Simplified Chinese version.

Chronic pain is a prevalent issue worldwide and is a significant contributor to human suffering and disability. The Graded Chronic Pain Scale-Revised has exhibited favorable reliability and validity. However, its applicability yet to be explored in China.

HCN channels sense temperature and determine heart rate responses to heat.

Heart rate increases with heat, [1-3] constituting a fundamental physiological relationship in vertebrates. Each normal heartbeat is initiated by an action potential generated in a sinoatrial nodal pacemaker cell. Pacemaker cells are enriched with hyperpolarization activated cyclic nucleotide-gated ion channels (HCN) that deliver cell membrane depolarizing inward current that triggers action potentials.

A Rapid and Accurate Quantitative Analysis of Cellulose in the Rice Bran Layer Based on Near-Infrared Spectroscopy.

Cultivating rice varieties with lower cellulose content in the bran layer has the potential to enhance both the nutritional value and texture of brown rice. This study aims to establish a rapid and accurate method to quantify cellulose content in the bran layer utilizing near-infrared spectroscopy (NIRS), thereby providing a technical foundation for the selection, screening, and breeding of rice germplasm cultivars characterized by a low cellulose content in the bran layer. To ensure the accuracy of the NIR spectroscopic analysis, the potassium dichromate oxidation (PDO) method was improved and then used as a reference method.