Microbiota dynamics and metabolic mechanisms in fermented sausages inoculated with Lactiplantibacillus plantarum and Staphylococcus xylosus.

Lactiplantibacillus plantarum and Staphylococcus xylosus are common starters for fermented sausages. Several studies have demonstrated the impact of these two strains on the quality of fermented sausages. However, the mechanism underlying the effects of these two microorganisms on co-cultivation in sausages remains unclear.

Selection of High-Affinity and Selectivity AFB Circular Aptamer for Biosensor Application.

Detecting trace amounts of aflatoxin B (AFB), one of the most toxic food contaminants, is crucial for efficiently preventing potential health risks. Circular aptamers are promising candidates for bioanalytical applications due to their enhanced biological and structural stability as well as their compatibility with rolling circle amplification (RCA). Herein, we employed a high-efficiency magnetic chain graphene oxide-based SELEX to generate circular aptamers that bind AFB with high affinity and selectivity.

Rapid indirect detection of N-lactoyl-phenylalanine using dual DNA biosensors based on solution-gated graphene field-effect transistor.

As obesity rates continue to rise, there is an increasing focus on reducing obesity through exercise. People are becoming more aware of the importance of weight loss through physical activity. However, the effectiveness of exercise can vary significantly among individuals, making it challenging to evaluate its impact.

Characterization, physicochemical properties, antioxidant activity and hypolipidemic activities of a polysaccharides from Lachnum YM40.

LEP-1a, a new polysaccharide fraction isolated and purified from Lachnum YM40, has a molecular weight of 24.29 kDa. LEP-1a's chemical composition investigation showed that mannose, galactose, and glucose made up the majority of its cosmetics.

Magnetic graphene-enhanced exonuclease III assisted amplification strategy driven carbon nanozyme for tri-mode detection of Escherichia coli O157:H7.

Ultra-precision point-of-care detection of Escherichia coli O157:H7 in foods is an important issue. Here, the detection sensitivity was improved by a signal cascade amplification strategy synergised by exonuclease III assisted isothermal amplification and reverse magnetic strategy. The double-stranded DNA formed by the aptamer and the target DNA as a sensing switch, avoiding the complex process of specific nucleic acid extraction.

Development of a Novel Electrochemical Immunosensor for Rapid and Sensitive Detection of Sesame Allergens Ses i 4 and Ses i 5.

Due to their lipophilicity and low content, the major sesame oleosin allergens, Ses i 4 and Ses i 5, are challenging to identify using conventional techniques. Then, a novel unlabeled electrochemical immunosensor was developed to detect the potential allergic activity of sesame oleosins. The voltammetric immunosensor was constructed using a composite of gold nanoparticles (AuNPs), polyethyleneimine (PEI), and multi-walled carbon nanotubes (MWCNTs), which was synthesized in a one-pot process and modified onto a glass carbon electrode to enhance the catalytic current of the oxygen reduction reaction.

Dynamics and Stability Mechanism of Lactoferrin-EPA During Emulsification Process: Insights from Macroscopic and Molecular Perspectives.

Although eicosapentaenoic acid (EPA) as a functional fatty acid has shown significant benefits for human health, its susceptibility to oxidation significantly limits its application. In this study, we developed a nanoemulsion of the lactoferrin (LTF)-EPA complex and conducted a thorough investigation of its macro- and molecular properties. By characterizing the emulsion with different LTF concentrations, we found that 1.

Differences in bacterial community structure and volatile flavor substance of industrial-scale tiger skin chicken feet stored at air packaging and vacuum packaging.

The aim of this study was to investigate the differences of quality indexes, bacterial community and volatile organic compounds (VOCs) of industrial-scale tiger skin chicken feet (TSCF) under air packaging (AP) and vacuum packaging (VP). The results showed that the pH, total volatile basic nitrogen, total number of bacterial colony, and sensory scores in VP group changed less than those in AP group during the storage period. Different packaging conditions also had significant effects on bacterial community at the genus levels.

Development of nucleus-targeted histone-tail-based photoaffinity probes to profile the epigenetic interactome in native cells.

Dissection of the physiological interactomes of histone post-translational modifications (hPTMs) is crucial for understanding epigenetic regulatory pathways. Peptide- or protein-based histone photoaffinity tools expanded the ability to probe the epigenetic interactome, but in situ profiling in native cells remains challenging. Here, we develop a nucleus-targeting histone-tail-based photoaffinity probe capable of profiling the hPTM-mediated interactomes in native cells, by integrating cell-permeable and nuclear localization peptide modules into an hPTM peptide equipped with a photoreactive moiety.

Effect of cold plasma and ultrasonic pretreatment on drying characteristics and nutritional quality of vacuum freeze-dried kiwifruit crisps.

The effect of ultrasound and plasma pretreatment on freeze-dried kiwifruit crisps was investigated in this study. Using unpretreated kiwifruit as a control group (CG), the effects of ultrasound (US), plasma-activated water (PAW), ultrasound combined with plasma-activated water (UPAW), plasma-jet (PJ), and ultrasound combined with plasma-jet (UPJ) on the quality of vacuum freeze-dried kiwifruit were investigated. The results showed that all the pretreatments could change the microstructure of the crisps.

A Disintegrin and Metalloproteinase 17 Disrupts Bovine Macrophage MER Proto-Oncogene Tyrosine Kinase Integrity to Impede Apoptotic Cell Clearance and Promote Inflammation in Clinical Mastitis.

In clinical mastitis of dairy cows, the abnormal accumulation of apoptotic cells (ACs) and subsequent secondary necrosis and inflammation pose significant concerns, with macrophage-mediated efferocytosis, crucial for ACs clearance, remaining unexplored in this context. In nonruminants, MER proto-oncogene tyrosine kinase (MERTK) receptors are essential for efferocytosis and A Disintegrin and Metalloproteinase 17 (ADAM17) is thought to play a role in regulating MERTK integrity. This study aimed to delineate the in situ role of efferocytosis in clinical mastitis, with a particular focus on the interaction between MERTK and ADAM17 in bovine macrophages.

Proinflammatory cytokines interleukin-18 and interleukin-6 mediate anorexia induction by trichothecene deoxynivalenol and its congeners.

As the common foodborne mycotoxins with the highest pollution rate, deoxynivalenol (DON, also named "vomitoxin") can harm the health of humans and animals by causing anorectic response. It has four congeners: 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), nivalenol (NIV), and fusarenon X (FX). These five mycotoxins have been associated with the detrimental effect on food intake.

Interaction and cross-contamination potential of prepared beef steak isolates Pseudomonas weihenstephanensis and Macrococcus caseolyticus in biofilms of dual-species.

This study evaluated the interactions between single or dual-species biofilms formed by dominant spoilage bacteria P. weihenstephanensis and M. caseolyticus isolated from refrigerated, spoilage prepared beef steaks at 4 °C and elucidated the interactive behavior of biofilm development in dual species.

Single-cell transcriptomic analysis and luteolin treatment reveal three adipogenic genes, including Aspn, Htra1 and Efemp1.

A comparative transcriptomic analysis in adipose stem and progenitor cells (ASPCs) between obese and lean mice might facilitate the identification of novel adipogenic genes. Here, we compare transcriptomic differences in the ASPCs of subcutaneous adipose tissue (SAT) between the mice fed on a high-fat-diet (HFD) and the chow diet (CD)-fed mice by analyzing three independent single-cell RNA sequencing datasets. Six differential genes, including three up-regulated genes Aspn, Rrbp1, Fbln2 and three down-regulated genes Htra1, Plpp3, Efemp1, are identified and confirmed in HFD-fed mice.

New insights into rice starch-gallic acid-whey protein isolate interactions: Effects of multiscale structural evolution and enzyme activity on starch digestibility.

In starch-based food where proteins and polyphenols coexist, the impact of protein on the inhibition of starch digestion by polyphenols is unclear. Therefore, the aim of this study was to investigate the impact of whey protein isolate (WPI) on the inhibition of rice starch digestion by gallic acid (GA) from the aspects of multiscale structure and enzyme activity. Rice starch-gallic acid-whey protein isolate complex (RS-GA-WPI) was formed predominantly by hydrogen bonding and hydrophobic interactions.

Schiff Base Mediated Food-Derived Peptide Supramolecular Self-Assembly as Curcumin Carriers.

The fusion assembly strategy of supramolecular chemistry combined with dynamic covalent chemistry has provided novel insights into the design of precision nutrition and intelligent drug delivery carriers. This work involved the development of a supramolecular self-assembly originating from entropy- and enthalpy-driven dynamic covalent bonding on Schiff bases between egg white-derived peptide Gln-Ile-Gly-Leu-Phe (QIGLF) and glutaraldehyde (GA), denoted QIGLF-GA. The assembly exhibited outstanding assembly characteristics and multiwavelength autofluorescence properties.

Structural characterization and protective effect against oxidative stress of Volvariella volvacea polysaccharide.

In the present study, a homogeneous polysaccharide (HVP-2) was isolated and purified from (Chinese mushroom) Volvariella volvacea. The structural characteristics and protective effect of HVP-2 were investigated. The results showed that HVP-2 was composed of mannose, glucose, galactose and arabinose in a molar ratio (mol%) of 59.

Impact of pH-Shifting and Autoclaving on the Allergenic Potential of Red Kidney Bean ( L.) Lectins.

The ingestion of red kidney bean products is hindered by the persistent allergenicity of lectins, even after autoclaving. This study examined the modification of lectin allergenicity in red kidney beans by pH-shifting and autoclaving treatments, utilizing BALB/c mouse sensitization, recirculating perfusion, and a bone marrow-derived dendritic cell (BMDC) model for allergenicity evaluation. Compared to autoclaving alone, combined pH-shifting and autoclaving reduced allergic symptoms in BALB/c mice, as evidenced by lower serum IgE, mMCPT-1, GM-CSF, HIS, IL-2, IL-4, IL-9, IL-13, and IL-17 levels and higher IgG1, IgG2a, IL-10, IFN-γ, and IFN-α cytokine release.

The improvement of gel properties and volatiles for frozen egg white melted assisted with ultrasound.

The variation in thawing time, deterioration behavior, secondary structure, surface hydrophobicity, and average particle size of frozen egg whites (EW) thawed with or without ultrasound were characterized to evaluate the effect of ultrasound on the gel properties and volatiles of egg white thermogel (EWG). The texture, water holding capacity, etc., gel properties and microstructure were well maintained in frozen EW thawed by ultrasound (UEW) resulted from the mitigation of deterioration behavior due to shorter melting time (reduced 91.

A study to reveal the synergistic bacteriostatic potential of egg white lysozyme with carvacrol at the molecular level.

The modification of proteins by bioactive molecules may have a synergistic enhancement effect on their respective functional properties. In this study, carvacrol (Car) was used to modify egg white lysozyme (LYZ) to improve its bacteriostatic ability. The results of microbiological experiments suggested that LYZ modified with Car had enhanced bacteriostatic activity against Escherichia coli (E.

Mussel-inspired highly adhesive carrageenan-based coatings with self-activating enhanced activity for meat preservation.

In order to solve the problem of poor adhesion of polysaccharide coatings in meat storage and inconvenient secondary spraying, which leads to poor preservation effect, this study was inspired by the property of mussels to adhere firmly to surfaces and design a bioactive composite coating. Here, curcumin-loaded zein nanoparticles (CZ NPs) were successfully prepared and incorporated into carrageenan-based biocomposite coatings for chilled meat preservation. The prepared curcumin-zein-riboflavin-carrageenan (CZRC) coating featured smooth spherical morphology and the solubility of hydrophobic substances, the adhesion and stability of the composite coating were respectively improved to 3.

Preparation of photo-crosslinked microalgae-carboxymethyl chitosan composite hydrogels for enhanced wound healing.

Integrating microalgae into wound dressings has proven effective in promoting chronic wound healing through photosynthesis-induced oxygen release. However, challenges such as high crosslinking temperatures and prolonged gel molding processes limit microalgae growth and reduce the overall therapeutic impact. In this work, inspired by cell-symbiotic photo-crosslinked hydrogels, we present a novel photo-crosslinked microalgae carboxymethyl chitosan composite hydrogel.

Exploring the dual role of anti-nutritional factors in soybeans: a comprehensive analysis of health risks and benefits.

Soybeans (Glycine max [L.] Merr.) are a globally significant crop, valued for their high protein content and nutritional versatility.