Insight into the structural deterioration of biosynthesized holoferritin upon thermal treatment.

Holoferritin is considered a promising iron supplement, yet its preparation is challenging due to low extraction efficiencies from natural sources and the potential for structural damage during in vitro mineralization. This study reported the in vivo biosynthesis of a highly stable holoferritin (bs-holoFt) in Escherichia coli a high iron-loading capacity (1213 Fe atoms/protein) and systematically characterized the impact of heat treatments (70-100 °C) on the protein's multi-level structure and dual functions. Results showed a clear, temperature-dependent degradation pathway, initiated by the loss of α-helical content (decreased from 77.

Biological control of Aspergillus flavus in peanut and maize kernel using Pantoea vagans BWL1.

Aspergillus flavus infenction of crops, which are common worldwide, are a significant threat to human and animal health. In this study, the antagonistic effects of Pantoea vagans strain BWL1 on A. flavus were analyzed.

Evaluation of Emulsion Gel Stabilized by Konjac Glucomannan as a Pork Fat Substitute: Effect on Microstructure, Water Distribution, and Oxidative Stability of Meat Patties.

This study aims to evaluate the use of the emulsion gel stabilized by konjac glucomannan (KGM) as a substitute for pork fat and investigates its effects on the quality attributes of meat patties. The substitution resulted in enhanced visual presentation and a more intricate protein network structure, while reducing the cooking loss of the meat patties. Notably, the textural properties of the patties remained unaffected with fat substitution levels ranging from 0% to 80%.

Mechanistic interplay between myofibrillar proteins and phospholipid during thermal degradation.

To investigate the interaction mechanism between phospholipids and myofibrillar proteins (MP) during thermal degradation, Gas chromatography-mass spectrometry combined with thermal gravimetric analysis was used to analyze the effect of MP on volatile compounds and mass loss during the thermal degradation of phospholipids (PLs) (phosphatidylcholine and phosphatidylethanolamine). The addition of MP increased the number of volatile organic compounds generated during the pyrolysis of phospholipids from 10 to 17, including 5 aldehydes, 1 alcohol, and 1 ketone. At 100 °C, the pyrolysis loss of the MP and PL mixture exhibited altered thermal degradation patterns compared to PL or MP alone, suggesting interfacial interactions between the two components.

Plasmon Resonance Energy Transfer for Molecular-Scale Tracking Receptor Dimerization and Apoptosis at the Single-Cell Level.

Plasmon resonance energy transfer (PRET) faces critical challenges in achieving precise molecular-scale distance control and non-perturbative operation within single live-cell environments, e.g., the inability to dynamically tune the donor-acceptor distance () at the single molecular dipole level.

Ultraelastic Nanomembrane with Controllable Hotspots and Enhanced Photon Recovery for Single-Molecule Surface-Enhanced Raman Scattering.

The controlled assembly of noble metal nanomaterials at water-oil interfaces holds significant potential for enhancing the sensitivity and stability of surface-enhanced Raman scattering (SERS) technology. However, developing highly efficient nanomaterial assembly techniques to create structures with controllable plasmonic hotspots and easily transferable functionalities remains a critical challenge. Herein, we propose a hydrophobic modification-enhanced float assembly strategy to fabricate an ultraelastic nanomembrane (UE-nanomembrane) by reducing adsorption kinetic barriers.

Isolation and Characterization of a Cold-Adapted Bacteriophage for Biocontrol of in Seafood.

() is a preeminent seafood-borne pathogen, imposing significant economic burdens on global aquaculture. The escalating prevalence of multidrug-resistant strains has accentuated the critical urgency for developing sustainable biocontrol strategies. In this study, a bacteriophage designated vB_VPAP_XY75 (XY75) was isolated and biologically characterized to establish an effective control against .

Aflatoxin B1 Promotes M2-like Macrophage Polarization via IL-6 Expression in Hepatocellular Carcinoma.

Background: The development of liver cancer in China is mainly caused by HBV, HCV infection, and exposure to aflatoxin. Especially in warm and humid southern regions, aflatoxin pollution poses a threat to health, emphasizing the urgency of research on related liver cancer.

Methods: Firstly, through in vivo experiments, the important role of AFB1 in mediating changes in the immune microenvironment of liver cancer has been preliminarily validated.

Performance-enhanced lateral flow immunoassay for rapid and ultrasensitive detection of aflatoxin M in dairy products using in-situ gold deposition-mediated signal amplification strategy.

Aflatoxin M (AFM) is a common mycotoxin in dairy products, leading to both acute and chronic poisoning and the detection requirement is much higher than that of AFB1. In this study, we proposed a performance-enhanced lateral flow immunoassay (PE-LFIA) using in-situ gold deposition-mediated signal amplification strategy for effectively monitoring AFM. Of significance, this sensing signal on both T line and C line of lateral flow strip is improved by simultaneous and direct growth and deposition of gold shells on the surface of AuNPs.

The role of melatonin in enhancing antioxidant capacity and mitigating high light-induced damage in Chlamydomonas reinhardtii.

Exposure to high light (HL) induces oxidative stress in Chlamydomonas reinhardtii (C. reinhardtii), leading to the accumulation of secondary metabolites while reducing biomass production. This study investigated how melatonin (MT) modulates growth and metabolite production in C.

Transmembrane nanogold energy transfer ruler enables synchronized projecting of receptor dimerization-phosphorylation signaling.

Nanoscale dimerization geometries dictate transmembrane receptor phosphorylation dynamics, yet current methods fail to directly correlate spatial receptor organization with real-time phosphorylation states . Receptor tyrosine kinase (RTK) activation, essential for cellular signaling, is dynamically controlled by dimerization at the nanoscale, but existing tools cannot simultaneously resolve spatial dimer configurations and phosphorylation activity. Here, we developed a transmembrane nanogold surface energy transfer ruler (T-Nanoruler) to enable tracking of RTK activation.

Multi-coupled field processing enhanced thermal stability of egg white protein condensates through hydration effect.

The application of egg white protein in the food industry is limited due to its poor thermal stability. In this study, thermally stable egg white protein (EWP) micro-nano condensate (200-300 nm) was prepared through shear-assisted preheating. After sterilization (98 °C, 5 min), these condensates maintained stable soluble protein concentration and turbidity, while control EWP showed significant changes (soluble protein: 43.

The Surface-Topography Challenge: A Multi-Laboratory Benchmark Study to Advance the Characterization of Topography.

Unlabelled: Surface performance is critically influenced by topography in virtually all real-world applications. The current standard practice is to describe topography using one of a few industry-standard parameters. The most commonly reported number is a, the average absolute deviation of the height from the mean line (at some, not necessarily known or specified, lateral length scale).

Novel synergistic modulation strategy for egg white protein and pumpkin seed protein gelation: structural characteristics, swallowing functionality, and formation mechanisms.

Background: Egg white protein (EWP) can be used to enhance the thermal gel properties of pumpkin seed protein (PSP), but the underlying molecular mechanism and potential for foods for individuals with dysphagia remain unexplored. This study aims to investigate the texture properties of PSP-EWP co-gelation.

Results: Egg white protein significantly improved disulfide bond formation (from 31.

Ovotransferrin ameliorated intestinal barrier dysfunction in DSS-induced ulcerative colitis mice via inhibition of PI3K-Akt/MAPK signaling pathways and modulation of tissue metabolism.

Ovotransferrin (OVT), a bioactive protein from egg white, has been demonstrated to exhibit anti-inflammatory efficacy and suppress the progression of ulcerative colitis. Nevertheless, its role in preserving intestinal barrier integrity has not been reported. This study investigated the role of OVT on intestinal barrier dysfunction in DSS-induced ulcerative colitis mice and its underlying mechanisms.

Heavy Metal Ion-Specific Effect of Chitosan Across Length Scales.

Heavy metal ions have a strong effect on macromolecules from their molecular structures to macroscopic behaviors. However, the underlying mechanism that connects different length scales remains poorly understood. In this study, we combine single-molecule experiments, theoretical calculations, simulations, and macroscopic analyses to investigate the interaction between a typical heavy metal ion, Pb, and a natural polysaccharide chitosan.

Interactions of starch with cv. 'Chuju' ethanolic extract and their effects on starch digestion in vitro and glycolipid metabolism in diabetic mice.

This study investigated the interactions between cv. 'Chuju' ethanolic extract (CMCE) and three types of starch (CS: corn starch; WS: wheat starch; BS: buckwheat starch), and their effects on starch digestion and glycolipid metabolism in diabetic mice. The results showed that CMCE interacted with starch via non-covalent interactions, altering the short-range ordered structure and forming composite surfaces with more and smaller pores, thereby significantly increasing resistant starch (RS) content ( < 0.

From Chinemys reevesii shell to trauma care: Preparation and characterization of chitosan-tortoiseshell collagen composite sponge with hemostasis property.

Effective hemostasis is a critical step in the treatment of injuries. The chitosan-tortoiseshell collagen composite sponge cross-linked by glutaraldehyde (C-G-TCS) was prepared. The potential of it as a biomedical material was explored through comparative analysis.

Unlocking the potential of microalgae-derived therapeutic carriers: Characteristics, types, and nanomedical applications.

Traditional drug delivery systems are constrained by limitations such as low drug-loading efficiency, immunogenicity, and functional simplicity, rendering them inadequate to address the demands of complex pathological scenarios. Microalgae have emerged as a promising alternative system, possessing not only health benefits derived from their bioactive compounds but also inherent properties that naturally align with delivery system requirements. These advantageous characteristics include exceptional biocompatibility, easily modifiable surface properties, and photosynthetic oxygen-generating capacity.

Comparative analysis of physicochemical properties and volatile flavor compounds of a novel brown soy yogurt prepared via Maillard browning reaction.

This study aimed to develop a low-beany Maillard brown soy yogurt (MBSY) and compare its physicochemical properties and flavor with non-Maillard soy yogurt, commercial soy yogurt, and commercial brown yogurt. Response surface optimization determined key process parameters for MBSY: glucose addition of 7.87%, glycine addition of 1.

Construction of OSA-starch and caffeic acid composite system driven by high pressure homogenization: Multiscale structural evolution and digestibility regulation mechanism.

The effect of modification treatments on the structure and function of starch-polyphenol complexes has become a hot research topic. Therefore, this study aims to systematically investigate the structural reorganization and digestibility modulation of octenyl succinic anhydride modified rice starch (OR)-caffeic acid complexes (ORCA) under gradient high pressure homogenization (HPH) pressures (50, 80, and 110 MPa). HPH promoted the interaction between starch and CA compared to the non-HPH-treated samples.

Antimicrobial mechanism of typical microbial preservatives and its potentiation strategy by photodynamic inactivation: A review.

Food safety faces challenges from microbial contamination, necessitating effective and consumer-friendly preservation methods. Traditional physical and chemical techniques have drawbacks, including reduced food quality and health risks. Microbial preservatives, such as bacteriocins, bacteriophages, and endolysins, offer natural alternatives but have limitations, including narrow spectra and slow action.

New insights into the regulation mechanism of starch digestibility by polyphenol structures in starch-polyphenol-protein ternary system.

The study aimed to investigate the effect mechanism of polyphenol structures on starch digestibility in the starch-polyphenol-protein ternary system. Polyphenol structures affected the affinity between polyphenols and whey protein isolate (WPI), with flavonoid-based polyphenols possessing a higher affinity to WPI. The law of polyphenol structures impacting the starch multiscale structure was altered in starch-polyphenol-protein ternary system compared to starch-polyphenol binary system.

Enhancing dry-fermented sausages: Meyerozyma guilliermondii as a flavor-boosting yeast in co-inoculation with Latilactobacillus sakei.

Background: Dry-fermented sausages are traditional meat products, and their quality and flavor largely depend on the complex microbial interactions during the fermentation process. Lactic acid bacteria (LAB) are widely used in the fermentation of sausages, but studies have shown that yeast has potential in terms of flavor complexity and process performance. Therefore, exploring the co-inoculation of yeast and LAB can provide new opportunities for optimizing the production of fermented sausages.