Exercise training ameliorates high-fat diet-induced skeletal muscle atrophy and ferroptosis via downregulation of STING.

Background: High-fat diet (HFD)-induced sarcopenic obesity can lead to reductions in muscle fiber diameter, enhanced protein degradation, and various forms of cell death. Exercise training has been shown to alleviate HFD-induced muscle atrophy. However, the underlying mechanism remains unclear.

A CRISPR/Cas12a mediated click immunoassay catalyzed by in situ formation of clickase for highly sensitive detection of Trichinella spiralis.

The accurate and high-throughput detection of trace protein targets remains an ongoing challenge in the field of food safety testing. This research article presents a highly sensitive CRISPR/Cas12a-mediated click immunoassay for the sensitive detection of Trichinella spiralis (T. spiralis) in meat samples.

Lipid droplets: Emerging therapeutic targets for age-related metabolic diseases.

Lipids metabolism is crucial in regulating aging and metabolic diseases. Lipid droplets (LDs) are dynamic, complex organelles responsible for the storage and release of neutral lipids, essential for maintaining lipid homeostasis and energy metabolism. Aging accelerates the accumulation of LDs, functional deterioration, and metabolic disorders, thereby inducing age-related metabolic diseases (ARMDs).

The Areas of Echinococcosis From the Qinghai-Tibet Plateau Extend to Yunnan: An Observation of Deworming and Control Integrated Impact Evaluation on Dogs.

is a zoonotic parasitic disease caused by the larval stage of the parasite Echinococcus, which has a long incubation period, expensive diagnosis and treatment, and long duration of medication, so it brings a great economic burden to patients and their families. Shangri-La City is one of the counties with a high prevalence of echinococcosis in Yunnan Province. The antigen-positive rate of Echinococcus feces is high and there is a potential risk of infecting people.

The transcription factors AdNAC3 and AdMYB19 regulate kiwifruit ripening through brassinosteroid and ethylene signaling networks.

The pivotal role of ethylene (ETH) in fruit ripening has been extensively studied; however, the function of brassinosteroids (BRs) in regulating fruit ripening remains poorly understood. Specifically, the mechanism by which BRs interact with ETH to affect kiwifruit (Actinidia deliciosa) ripening is unclear. Our research showed that 2 genes encoding transcription factors, AdNAC3 and AdMYB19, and the fruit softening gene AdEXP3 (encoding a cell wall expansion protein, expansin 3) were upregulated by ETH and downregulated by BRs.

Past, present and future epidemiology of echinococcosis in China based on nationwide surveillance data 2004-2022.

Objectives: We evaluated the epidemiological characteristics of echinococcosis, a global public health threat, in China to inform global control efforts.

Methods: Descriptive, statistical, cluster, spatial, and trend analyses were used to evaluate the epidemiology at national, provincial, and county levels based on 2004-2022 nationwide surveillance data from China.

Results: Between 2004 and 2022, China recorded 72,676 cystic echinococcosis (CE) cases, 11,465 alveolar echinococcosis (AE) cases, and 5703 others, with an average annual cases per million (ANpM) of 3.

The unique activity of the bone morphogenetic protein TGH4 affects the embryonic development of Trichinella spiralis and the establishment of vaccine protection.

In recent years, animal outbreaks of trichinellosis have been reported in Mexico, China, Algeria, Croatia and others, which is a challenge to meat safety and public health. Vaccination is an important means to control trichinellosis, but one of the main challenges in vaccine development is screening for protective candidate antigens. Bone morphogenetic proteins (BMP)), which are potential vaccine research targets, play key roles in the growth and development of metazoans.

Bidirectional mediation of psychological distress and food addiction influenced by childhood trauma among adolescents.

Background: This study aims to explore the relationship between childhood trauma, psychological distress, and food addiction in adolescents.

Methods: The sample comprised 4560 adolescents (46.67 % females) aged 12-19 years (15.

Molecular characterization and B-cell epitope analysis of the TSP11 gene in infection strains from Yunnan Province.

This study investigates the molecular intricacies of the transmembrane protein TSP11 gene in strains isolated from livestock and patients in Yunnan Province afflicted with between 2016 and 2020. Gene typing analysis of the ND1 gene revealed the presence of the G1 type, G5 type and untyped strains, constituting 52.4, 38.

Analysis of Prevalence and Related Factors of Cyberbullying-Victimization among Adolescents.

Cyberbullying is an increasingly serious issue that negatively impacts the mental and physical health of adolescents. This study aims to report the prevalence rates of adolescent cyberbullying-victimization and its associated related factors, providing a scientific basis for targeted efforts to protect the mental and physical well-being of adolescents; Methods: From March to May 2019, there were 13 high schools and 33 middle schools in Yixing, with a student ratio of 2:1 between middle and high school. Using a random cluster sampling method, we selected four high schools and three middle schools based on this ratio, resulting in a total of 13,258 students.

The chromodomain protein CDYL confers forebrain identity to human cortical organoids by inhibiting neuronatin.

Fate determination of neural stem cells (NSCs) is crucial for cortex development and is closely linked to neurodevelopmental disorders when gene expression networks are disrupted. The transcriptional corepressor chromodomain Y-like (CDYL) is widely expressed across diverse cell populations within the human embryonic cortex. However, its precise role in cortical development remains unclear.

Occurrence rate and species and subtypes of Cryptosporidium spp. in pet dogs in Yunnan Province, China.

Background: Cryptosporidium spp. is a ubiquitous, globally distributed intestinal protozoan infecting humans and at least 260 animal hosts. Due to close human contact with pet dogs and identification of zoonotic Cryptosporidium species and subtypes in these animals, dog health is not only a veterinarian issue but also a public health issue.

Does participation in sports competitions enhance interprofessional teamwork among medical students? Evidence from a medical school curriculum experiment.

Background: Effective interprofessional teamwork is essential for the efficiency, safety and quality of healthcare system services and requires interprofessional education for medical students. Physical education is a simple and easy way to teach teamwork, which translates into team performance in the work environment. This study was conducted to examine the effectiveness of the physical education competition model, instead of the exams model, for improving teamwork skills among medical students.

Revealing the dynamic whole transcriptome landscape of Clonorchis sinensis: Insights into the regulatory roles of noncoding RNAs and microtubule-related genes in development.

Clonorchis sinensis is a significant zoonotic food-borne parasite that causes a range of hepatobiliary diseases, which in severe cases can even lead to cholangiocarcinoma. To explore new diagnostic and treatment strategies, the dynamic RNA regulatory processes across different developmental stages of C. sinensis were analyzed by using whole-transcriptome sequencing.

The effect of β-Glucan induced intestinal trained immunity against Trichinella spiralis infection.

Parasitic helminth Trichinella spiralis (Ts) induce mixed Th1/Th2 response with predominant type 2 immune responses, with protective immunity mediated by interleukin (IL)-4, IL-5, and IL-13. β-Glucan (BG) has been shown to have the ability to induce trained immunity, confers non-specific protection from secondary infections. However, whether BG-induced trained immunity played a role in protective type 2 immunity against Ts infection is unclear.

Trichinella spiralis alleviates LPS-induced acute lung injury by modulating the protective Th2 immune response.

Sepsis is a disorder of immune regulation caused by pathogenic microorganisms. A large number of inflammatory factors and inflammatory mediators are released, resulting in systemic inflammatory response disorder and acute lung injury (ALI). Helminths infection activate Th2 cytokines and immunomodulatory pathways, which have the function of anti-infection effector molecules.

Occurrence and genetic characterization of Enterocytozoon bieneusi in pet dogs in Yunnan Province, China.

Enterocytozoon bieneusi is the most common microsporidian species in humans and can affect over 200 animal species. Considering possible increasing risk of human E. bieneusi infection due to close contact with pet dogs and identification of zoonotic E.

45 activates SHP2 through inhibition of oxidative stress to regulate osteoblast and osteoclast differentiation.

Background: The purpose of this study is to observe LP45 ( 45) to investigate the mechanism by which LP45 attenuates oxidative stress-induced damage and regulates the osteoblast-osteoclast balance.

Materials And Methods: The oxidative stress level and osteoblast- and osteoclast-related proteins were detected by immunofluorescence staining, Western blotting, ROS fluorescent probe and ELISA. Osteoblast cell proliferation capacity was determined by the CCK-8 assay.

Agarose Hydrogel-Boosted One-Tube RPA-CRISPR/Cas12a Assay for Robust Point-of-Care Detection of Zoonotic Nematode Anisakis.

Rapid and accurate detection of the zoonotic nematode Anisakis is poised to control its epidemic. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas-associated assay shows great potential in the detection of pathogenic microorganisms. The one-tube method integrated the CRISPR system with the recombinase polymerase amplification (RPA) system to avoid the risk of aerosol pollution; however, it suffers from low sensitivity due to the incompatibility of the two systems and additional manual operations.

Quantitative label-free proteomic analysis of excretory-secretory proteins in different developmental stages of Trichinella spiralis.

Trichinella spiralis (T. spiralis) is a zoonotic parasitic nematode with a unique life cycle, as all developmental stages are contained within a single host. Excretory-secretory (ES) proteins are the main targets of the interactions between T.

Epidemiological characteristics and risk factors for cystic and alveolar echinococcosis in China: an analysis of a national population-based field survey.

Background: Human cystic and alveolar echinococcosis are neglected tropical diseases that WHO has prioritized for control in recent years. Both diseases impose substantial burdens on public health and the socio-economy in China. In this study, which is based on the national echinococcosis survey from 2012 to 2016, we aim to describe the spatial prevalence and demographic characteristics of cystic and alveolar echinococcosis infections in humans and assess the impact of environmental, biological and social factors on both types of the disease.

Transcriptome and Metabolomics Integrated Analysis Reveals Associated with Esters Biosynthesis in Apple ( × ).

Volatile esters are major aromas contributing to the organoleptic quality of apple fruit. However, the molecular mechanisms underlying the regulation of volatile ester biosynthesis in apple remain elusive. This study investigated the volatile profiles and transcriptomes of 'Qinguan' (QG) apple fruit during development and/or postharvest storage.