Tectorigenin attenuates cardiac hypertrophy via USP9X/MCL1-mediated mitochondrial stabilization.

Pathological cardiac hypertrophy, driven by mitochondrial dysfunction and maladaptive remodeling, remains a therapeutic challenge. This study explores the cardioprotective properties of tectorigenin (Tec) in the context of transverse aortic constriction (TAC)-induced hypertrophy, focusing on mitochondrial homeostasis. In animal models, administration of Tec improved survival rates, reduced cardiac dysfunction, and decreased hypertrophy and fibrosis in TAC mice, while preserving mitochondrial function.

DDX60 protects ischemic myocardial injury and heart dysfunction by improving mitochondrial function via promoting Arl2 mRNA translation.

Mitochondrial dysfunction is increasingly recognized as a pivotal driver of cardiomyocyte apoptosis and cardiac deterioration following myocardial infarction (MI). This study identifies a significant upregulation of DDX60 in cardiomyocytes under hypoxic conditions. Elevated DDX60 levels enhance mitochondrial function and attenuate cardiomyocyte apoptosis in vitro, whereas its knockdown induces the opposite effects.

Machine learning-based analysis and prediction of factors influencing mental health among children and adolescents in Jiangsu Province.

Background: This study investigates the current mental health status among children and adolescents in Jiangsu Province by analyzing symptoms of depression, anxiety, and stress using standardized psychological scales. Machine learning models were utilized to identify key influencing variables and predict mental health outcomes, aiming to establish a rapid psychological well-being assessment framework for this population.

Objective: A cross-sectional survey was conducted via random cluster sampling across 98 counties (cities/districts) in Jiangsu Province, enrolling 141,725 students (47,502 primary, 47,274 junior high, 11,619 vocational high school students, and 35,330 senior high ).

Comparative Effectiveness of Exercise Modalities for Fibromyalgia: A Systematic Review and Bayesian Network Meta-Analysis.

Objective: To synthesise existing evidence assessing the impact of exercise-based therapies on pain in patients with fibromyalgia syndrome (FM), determine the efficacy of various exercise modalities, and establish the optimal exercise dosage for pain management.

Design: Systematic review and network meta-analysis.

Data Sources: MEDLINE, Embase, Web of Science, Cochrane Library, Scopus and SPORT Discus were searched from inception to July 2024.

Charting the spatial transcriptome of the human cerebral cortex at single-cell resolution.

A high-resolution spatial physiological atlas of cortical neurons serves as an essential reference for studying neurological diseases and is crucial for a comprehensive understanding of the cortical functions and physiological mechanisms. Although previous studies have elucidated the cellular basis of individual cortex, the molecular characteristics and precise spatial organization of neural cells within multiple human cortex remain incompletely understood. Subcellular-level spatial transcriptomic sequencing and snRNA-seq enabled mapping of 14 human cortical regions, creating a single-cell atlas with transcriptomic data from 1,121,772 nuclei and spatial profiles from 1,888,306 cells to characterize neural cell organization.

Differentiating between adrenocortical carcinoma and pheochromocytoma by a CT-based radiomics model: a multicenter retrospective study.

Background: Adrenocortical carcinoma (ACC), a rare and highly malignant adrenal gland tumor, exhibits computed tomography (CT) characteristics that resemble those of the less malignant pheochromocytoma (PHEO). While biochemical evaluation is widely accepted for differentiating between ACC and PHEO, non-functioning tumors remain a diagnostic challenge. The similarity in CT imaging and atypical hormone levels can lead to suboptimal accuracy in diagnosis, leading to inappropriate clinical interventions.

Ventricular-arterial coupling is preserved in prematurely born 11-year-old children but calls for life-long prevention of hemodynamic deterioration.

Background: Premature birth disrupts the intra-uterine structural and functional maturation of the left ventricle (LV) and arteries. The study investigated the impact of premature birth on ventricular-arterial coupling (VAC), a potential precursor of cardiovascular disease in adulthood.

Methods: This case-control study in Northern Belgium (2011-2016) included 93 extremely-low-birth-weight (ELBW) cases and 87 sex and age-matched term-born controls.

Reallocating diabetes-related garbage codes to improve mortality estimates: a case study in Weifang, China.

Effective identification and correction of diabetes mellitus (DM)-related garbage codes (GCs) in mortality surveillance data is crucial for accurately estimating regional DM mortality rates. This study applied a structured, three-step approach-using standard WHO ICD-10 mortality coding rules, coarsened exact matching (CEMM), and fixed proportion reassignment (FPRM)-to redistribute diabetes-related GCs in Weifang's mortality data (2010-2022). Using ICD-10 coding rules, we reclassified 29 deaths originally assigned to DM as the underlying cause of death (UCD) to other causes, and reassigned 1,945 records previously not attributed to DM to DM as the UCD.

TriCLFF: a multi-modal feature fusion framework using contrastive learning for spatial domain identification.

Spatial transcriptomics (ST) encompasses rich multi-modal information related to cell state and organization. Precisely identifying spatial domains with consistent gene expression patterns and histological features is a critical task in ST analysis, which requires comprehensive integration of multi-modal information. Here, we propose TriCLFF, a contrastive learning-based multi-modal feature fusion framework, to effectively integrate spatial associations, gene expression levels, and histological features in a unified manner.

Benchmarking metabolic RNA labeling techniques for high-throughput single-cell RNA sequencing.

Metabolic RNA labeling with high-throughput single-cell RNA sequencing (scRNA-seq) enables precise measurement of gene expression dynamics in complex biological processes, such as cell state transitions and embryogenesis. This technique, which tags newly synthesized RNA for detection through induced base conversions, relies on conversion efficiency, RNA integrity, and transcript recovery. These factors are influenced by the chosen chemical conversion method and platform compatibility.

Electrochemical glycoproteins sensor for sensitive diagnosis of PD: Ag@MSNs-MPBA as a specific signal amplifier and reporter.

Glycoprotein non-metastatic melanoma protein B (GPNMB) is a candidate biomarker of Parkinson's disease (PD). The development of a novel method for highly sensitive and specific detection of GPNMB is essential for PD's early and accurate diagnosis. An electrochemical biosensor was developed utilizing silver nanoparticle-loaded mesoporous silica (Ag@MSNs) with 4-mercaptophenylboronic acid (4-MPBA) modification for the sensitive and selective detection of GPNMB.

IL-4-STAT6-induced high Siglec-G/10 expression aggravates the severe immune suppressive tumor microenvironment and impedes the efficacy of immunotherapy in head and neck squamous cell carcinoma.

Background: Immune checkpoint blockade therapy has shown limited efficacy in head and neck squamous cell carcinoma (HNSCC). Sialic acid binding immunoglobulin-like lectin (Siglec)-15 has been identified as a novel immune evasion biomarker, while the role of Siglec-10 in the specific immune suppressive tumor microenvironment remains largely unknown.

Methods: Immunohistochemical assays were employed to investigate the correlation of the expressions of Siglec-10 and Siglec-15 with the clinicopathological features as well as the prognosis of immunotherapy in patients with HNSCC.

Identifying T cell antigen at the atomic level with graph convolutional network.

Precise identification of T cell antigens in silico is crucial for the development of cancer mRNA vaccines. However, current computational methods only utilize sequence-level rather than atomic level features to identify T cell antigens, which results in poor representation of those that activate immune responses. Here we propose deepAntigen, a graph convolutional network-based framework, to identify T cell antigens at the atomic level.

Recent Advances of Fluorescent Aptasensors for the Detection of Antibiotics in Food.

Antibiotics can accumulate in the body via ingestion, presenting serious health and safety risks to humans, and have garnered extensive international attention in recent years. Meanwhile, aptamers have been applied in the detection of antibiotics, mainly because of their good stability, high specificity, easy synthesis, and low cost. Among various kinds of aptasensors, fluorescent dye-based or nanomaterial-based fluorescent aptasensors serve as highly efficient tools for the rapid quantification of antibiotics owing to their remarkable sensitivity, specificity, and selectivity.

Age-Dependent Associations Between Pulse Pressure and Long-Term Outcomes After Myocardial Infarction.

Pulse pressure (PP) is a recognized marker of cardiovascular risk in the general population. However, its role as an independent predictor of recurrent cardiovascular events following myocardial infarction (MI) and whether there are age-dependent differences in this relationship remains uncertain. We analyzed data from 4091 participants with a history of MI were enrolled in the Kailuan Study.

Ambulatory blood pressure monitoring, European guideline targets, and cardiovascular outcomes: an individual patient data meta-analysis.

Background And Aims: Hypertension is the predominant modifiable cardiovascular risk factor. This cohort study assessed the association of risk with the percentage of time that the ambulatory blood pressure (ABP) is within the target range (PTTR) proposed by the 2024 European Society of Cardiology (ESC) guidelines for blood pressure (BP) management.

Methods: In a person-level meta-analysis of 14 230 individuals enrolled in 14 population cohorts, systolic and diastolic ABPs were combined to assess 24-h, daytime, and nighttime PTTR with thresholds for non-elevated ABP set at <115/65, <120/70, and <110/60 mmHg, respectively.

Serum Proteomics Analysis of Patients with Ascending Aortic Dilation.

Ascending aortic dilation (AAD) is a complex and life-threatening condition, representing a significant risk factor for acute aortic syndromes. Due to its asymptomatic nature, early diagnosis is frequently missed. Serum diagnostic biomarkers play a crucial role in disease diagnosis, and proteomics offers a valuable approach for identifying such biomarkers in blood samples.

Deletion of hepcidin disrupts iron homeostasis and hematopoiesis in zebrafish embryogenesis.

Iron is essential for cell growth and hematopoiesis, which is regulated by hepcidin (hamp). However, the role of hamp in zebrafish hematopoiesis remains unclear. Here, we have created a stable hamp knockout zebrafish model using the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 system (CRISPR/Cas9 system).

Variation in and Factors Associated with Youth Self-Harm in College Students - Jiangsu Province, China, 2019-2023.

What Is Already Known About This Topic?: Self-harm represents a significant public health challenge that disproportionately affects young adults. Understanding individual- and institutional-level risk factors is crucial for developing and implementing effective mental wellness intervention programs for college students.

What Is Added By This Report?: The prevalence of youth self-harm fluctuated between 2.

Bidirectional mediation of psychological distress and food addiction influenced by childhood trauma among adolescents.

Background: This study aims to explore the relationship between childhood trauma, psychological distress, and food addiction in adolescents.

Methods: The sample comprised 4560 adolescents (46.67 % females) aged 12-19 years (15.

Health risks related to environmental and occupational lead exposure.

Lead is a ubiquitous toxic metal. This review summarizes the toxicokinetics and toxicology of lead, and the methods to measure blood lead (BL), summarizes recently published studies, and ends with an appraisal of recent regulations to protect exposed workers. Lead exposure is caused via inhalation of lead-contaminated particulate and via gastrointestinal uptake.

Efficacy and safety of gut microbiome-targeted treatment in patients with depression: a systematic review and meta-analysis.

Background: The study aimed to comprehensively analyze and establish a framework for evaluating the efficacy of microbiome-targeted treatment (MTT) for depression.

Methods: We searched PubMed, Embase, Cochrane Library, Web of Science, and the Chinese National Knowledge Infrastructure database for randomized controlled trials (RCTs) on MTT in treating depression until October 19, 2023. A meta-analysis was conducted to evaluate the efficacy and safety of MTT.

Association of Total Mortality and Cardiovascular Endpoints With the Timing of the First and Second Systolic Peak of the Aortic Pulse Wave.

Prognostic significance of the timing in the cardiac cycle of the first (TP1) and second (TP2) systolic peak of the central aortic pulse wave is ill-defined. Incidence rates and standardized multivariable-adjusted hazard ratios (HRs) of adverse health outcomes associated with TP1 and TP2, estimated by the SphygmoCor software, were assessed in the International Database of Central Arterial Properties for Risk Stratification (IDCARS) (n = 5529). Model refinement was assessed by the integrated discrimination (ID) and net reclassification (NR) improvement.