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Volatile esters are major aromas contributing to the organoleptic quality of apple fruit. However, the molecular mechanisms underlying the regulation of volatile ester biosynthesis in apple remain elusive. This study investigated the volatile profiles and transcriptomes of 'Qinguan' (QG) apple fruit during development and/or postharvest storage. Although the constitution of volatiles varied widely between the peel and flesh, the volatile profiles of the peel and flesh of ripening QG fruit were dominated by volatile esters. WGCNA results suggested that 19 genes belonging to ester biosynthesis pathways and 11 hub transcription factor genes potentially participated in the biosynthesis and regulation of esters. To figure out key regulators of ester biosynthesis, correlation network analysis, dual-luciferase assays, and yeast one-hybrid assay were conducted and suggested that MdMYB94 trans-activated the promoter and participated in the regulation of ester biosynthesis. This study provides a framework for understanding ester biosynthesis and regulation in apple.
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http://dx.doi.org/10.1021/acs.jafc.2c07719 | DOI Listing |
Pestic Biochem Physiol
November 2025
Departamento de Entomologia, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, Brazil. Electronic address:
Managing Euschistus heros is increasingly challenging due to insecticide resistance, limited available molecules, and environmental and health concerns. Here, we synthesized and evaluated mucochloric acid derivatives as alternative tools for controlling this pest. We assessed the selectivity of these molecules toward the pollinators bees Apis mellifera and Partamona helleri and conducted in silico predictions for the interactions of these molecules with receptors for γ-aminobutyric acid (GABARs) and nicotinic acetylcholine receptors (nAChRs), and the enzyme acetylcholinesterases (AChE) of E.
View Article and Find Full Text PDFPestic Biochem Physiol
November 2025
Department of Biology & CESAM-Centre for Environmental and Marine Studies, University of Aveiro, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal.
Maize (Zea mays L.) is one of the world's most widely cultivated and economically important cereal crop, serving as a staple food and feed source in over 170 countries. However, its global productivity is threatened by late wilt disease (LWD), a disease caused by Magnaporthiopsis maydis, that spreads through soil and seeds and can cause severe yield losses.
View Article and Find Full Text PDFCarbohydr Polym
November 2025
Engineering Research Center for Biomedical Materials, Anhui Key Laboratory of Modern Biomanufacturing, School of Life Sciences, Anhui University, 111 Jiulong Road, Hefei, Anhui Province 230601, PR China. Electronic address:
The primary objective of this study was to develop a nanosuspension based on orthoester compounds (OE) and carboxymethyl chitosan (CMCS) for the combined treatment of tumors. Initially, OE was synthesized as a liquid pharmaceutical excipient. Subsequently, nanoparticles were formulated using CMCS and loaded with mitoxantrone (MIT).
View Article and Find Full Text PDFProc Natl Acad Sci U S A
September 2025
Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel.
We applied micro-computed tomography, high-resolution cryo-scanning electron microscopy (SEM) combined with cathodoluminescence, and cryo-focused ion beam Milling-SEM to perform three-dimensional imaging of human atherosclerotic tissues with tens of nanometers resolution, under hydrated, near-native conditions with minimal sample processing. The same technology was applied to cultured macrophages exposed to cholesterol crystals, and the observations made on the macrophages were compared to those made on the pathological tissue. We observed that cholesterol crystal digestion and, eventually, cholesterol crystal clearance occurs in the advanced human plaques through cellular processing.
View Article and Find Full Text PDFEur Heart J
September 2025
Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Milan, Italy.
Cardiovascular disease remains a major global health challenge, with dyslipidaemia being a key modifiable risk factor. While low density lipoprotein cholesterol (LDL-C) is the primary target for lipid-lowering therapies, recent evidence highlights the importance of triglycerides, apolipoprotein B (apoB), and lipoprotein(a) [Lp(a)] for residual cardiovascular risk. Current lipid-lowering therapies target key enzymes and proteins involved in cholesterol and lipid metabolism.
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