Fixed-Time Active Disturbance Rejection Temperature-Pressure Decoupling Control for a High-Flow Air Intake System.

High-flow aeroengine transient tests involve strong coupling and external disturbances, which pose significant challenges for intake environment simulation systems (IESSs). This study proposes a compound control scheme that combines fixed-time active disturbance rejection with static decoupling methods. The scheme integrates a fixed-time sliding-mode controller (FT-SMC) and a super-twisting fixed-time extended-state observer (ST-FT-ESO).

De novo design mini-binder proteins targeting the capsid-forming domain of type 2 PRRSV nucleocapsid protein to inhibit PRRSV replication in vitro.

Porcine reproductive and respiratory syndrome virus (PRRSV) has caused huge economic losses to swine industry but there is no effective antiviral drug. Nucleocapsid (N) protein is highly conserved in type 2 PRRSV and is considered as an important target for antiviral development. Mini-binders are novel protein drugs de novo designed for a specific protein target using a computational approach, which has great application prospects.

Optimization of a weight matrix calculation for computed tomography of chemiluminescence imaging.

Computed tomography of chemiluminescence (CTC) reconstructs unknown physical quantities in 3D fluids by capturing 2D projections, and the imaging model must accurately describe the mathematical relationship between the volume of interest (VoI) and the projections. This paper addresses the optimization of the weight matrix calculation in CTC imaging and introduces the Monte Carlo subpixel (MC-subpixel) method. The method is applied to dynamic imaging scenarios where frequent camera position adjustments are required, such as scenes with limited projection angles or opaque optical obstructions.

Efficacy and safety of small-molecule TKIs in neoadjuvant treatment of HER2-positive breast cancer: a systematic review and network meta-analysis.

Objective: This study aimed to evaluate the efficacy and safety of small-molecule TKIs in neoadjuvant treatment of HER2-positive breast cancer using a network meta-analysis approach.

Methods: A systematic literature search was conducted in the Medline, Embase, and Web of Science databases. Eligible studies that included HER2-positive breast cancer patients receiving neoadjuvant treatment with small-molecule TKIs before surgery were included.

Improving the Catalytic Activity and Thermostability of FAST-PETase with a Multifunctional Short Peptide.

Previous reports indicated that self-assembling amphipathic peptide S1v1 (AEAEAHAH) significantly enhances the soluble expression, thermostability, and activity of the target proteins when fused to them. In order to obtain high-efficiency enzymes for the large-scale degradation of polyethylene terephthalate (PET), this multifunctional peptide was fused to the N- and C-terminus of FAST-PETase, a variant of PETase (PETase), with a PT-linker (TTVTTPQTS) harbored between the target protein and the multifunctional peptide. Consistent with previous reports, S1v1 increased the solubility of FAST-PETase slightly.

Establishing a Novel Heterologous Secretion Expression System Mediated by mScarlet3 for the Expression of a Novel Lipolytic Enzyme.

Our previous study demonstrated that an heterologous secretion expression system, mediated by superfolder green fluorescent protein (sfGFP) mutants, significantly enhances recombinant lipase yield and reduces large-scale production costs. In this study, we identified mScarlet3, a fast-folding fluorescent protein, as another effective mediator of secretion expression in . A novel lipolytic enzyme, named LipHu6, was identified through sequence alignment.

Argonaute-driven programmable multi-enzyme complex assembly on ribosomal RNA scaffolds.

Scaffold-based multi-enzyme assembly strategies have significantly advanced biocatalysis by enhancing reaction efficiency through precise spatial organization of enzymes. While DNA- and protein-based scaffolds have been extensively studied, RNA scaffolds present unique advantages, including structural flexibility, dynamic regulation, and functional diversity. However, their application in vitro has been limited due to challenges related to stability and cost.

dPAFS: A nuclease-dead PfAgo-mediated fluorescence sensing platform for genotyping genome-edited rice.

Pyrococcus furiosus Argonaute (PfAgo)-mediated nucleic acid detection is a powerful tool for molecular diagnostics, offering ultrahigh sensitivity and single-nucleotide specificity. However, existing PfAgo-based methods require multi-step cleavage processes and the use of molecular beacons, which are cumbersome and costly. Herein, we developed a novel fluorescence sensing platform (dPAFS) based on a nuclease-dead PfAgo mutant (dPfAgo) to simplify PfAgo-mediated detection system significantly.

A distant TANGO1 family member promotes vitellogenin export from the ER in .

Vitellogenin is thought to share a common ancestor with human apolipoprotein B (ApoB) for systemic lipid transport. In , although a general route for inter-tissue vitellogenin transport has been described, the full mechanism that underlies its intracellular trafficking within the intestine remains obscure. In humans, the TANGO1 family of proteins generates membrane carriers to accommodate bulky ApoB-containing lipoprotein particles for their endoplasmic reticulum (ER) export.

New Species and Records Expand the Checklist of Cellular Slime Molds (Dictyostelids) in Jilin Province, China.

Dictyostelids represent a crucial element in the protist community, and their abundant presence in Jilin Province underscores their indispensable role in biodiversity conservation. In the present study, a resource survey of dictyostelids used random sampling to collect 28 soil samples from five localities in Changbai Korean Autonomous County, Jilin Province. In addition, a compilation of dictyostelid species reported from Jilin Province was developed, based on a thorough review of the literature.

Trends and hotspots on the relationship between gut microbiota and Parkinson's Disease: a bibliometric analysis.

Parkinson's disease (PD) is a neurodegenerative disorder that significantly impacts patients' quality of life. Recent evidence has highlighted a complex relationship between the gut microbiota (GM) and PD. Understanding this relationship is crucial for potentially targeting GM in PD treatment and expanding therapeutic options.

Structural and mechanistic insights into a mesophilic prokaryotic Argonaute.

Argonaute (Ago) proteins are programmable nucleases found in all domains of life, playing a crucial role in biological processes like DNA/RNA interference and gene regulation. Mesophilic prokaryotic Agos (pAgos) have gained increasing research interest due to their broad range of potential applications, yet their molecular mechanisms remain poorly understood. Here, we present seven cryo-electron microscopy structures of Kurthia massiliensis Ago (KmAgo) in various states.

Mechanism of multifunctional adaptor protein SHARPIN regulating myocardial fibrosis and how SNP mutation affect the prognosis of myocardial infarction.

Myocardial fibrosis (MF) is characterized by the excessive deposition of extracellular matrix within the heart, often following a cardiovascular insult. SHARPIN, a protein implicated in fibrosis, has emerged as a potential therapeutic target. This study aimed to elucidate the molecular mechanisms of SHARPIN in MF and to investigate the influence of its single nucleotide polymorphism (SNP), rs117299156, on myocardial infarction (MI) patients.

Slime molds (Myxomycetes) causing a "disease" in crop plants and cultivated mushrooms.

Myxomycetes (plasmodial slime molds) are eukaryotic protist predators that are associated with wood, leaf litter, and soil in forests, where they feed on bacteria, protozoans, and (to a more limited extent) fungi. The health of crop plants is essential because they represent a primary food source for humans. However, when myxomycetes produce numerous fruiting bodies on the stems and leaves of crop plants, which is herein referred to as a myxomycete colonization, this has the potential of interfering with plant photosynthesis, transpiration and respiration by blocking out light and covering stomata.

A Whole-Process Visible Strategy for the Preparation of Rhizomucor miehei Lipase with Escherichia coli Secretion Expression System and the Immobilization.

Background: Rhizomucor miehei (RM) lipase is a regioselective lipase widely used in food, pharmaceutical and biofuel industries. However, the high cost and low purity of the commercial RM lipase limit its industrial applications. Therefore, it is necessary to develop cost-effective strategies for large-scale preparation of this lipase.

Preamplification-free ultra-fast and ultra-sensitive point-of-care testing via LwaCas13a.

CRISPR based nucleic acid detection technology provides a deployable approach to point of care testing. While, there remain challenges limiting its practical applications, such as the need for pre-amplification and the long turnaround time. Here, we present a self-cascade signal amplification method with LwaCas13a and an artificially designed "U" rich RNA of stem-loop structure (URH) for pre-amplification-free ultra-fast and ultra-sensitive point-of-care testing (PASSPORT).

Fast and practical method for underwater stereo vision calibration based on ray-tracing.

The perspective camera model has difficulty handling refracted light in the underwater environment. To achieve accurate and convenient calibration in large underwater scenes, we propose a method based on the underwater refractive camera model in this paper. First, the initial values of the refraction parameters are solved using refraction coplanarity constraints.

TLTC, a T5 exonuclease-mediated low-temperature DNA cloning method.

Molecular cloning is used in a wide variety of biological and medical research. Here, we developed a rapid and efficient DNA-assembling method for routine laboratory work. We discovered that the cleavage speed of T5 exonuclease is approximately 3 nt/min at 0°C and hence developed a T5 exonuclease-mediated low-temperature sequence- and ligation-independent cloning method (TLTC).

Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly.

Programmable endonucleases, such as Cas (Clustered Regularly-Interspaced Short Repeats-associated proteins) and prokaryotic Argonaute (pAgo), depend on base pairing of the target DNA with the guide RNA or DNA to cleave DNA strands. Therefore, they are capable of recognizing and cleaving DNA sequences at virtually any arbitrary site. The present review focuses on the commonly used in vivo and in vitro recombination-based gene cloning methods and the application of programmable endonucleases in these sequence- and ligation-independent DNA assembly methods.

Severe hyperlipidemia pancreatitis induced by taking tamoxifen after breast cancer surgery-Case report.

Introduction: The research investigates the mechanism, diagnosis, treatment, and subsequent endocrine therapy of severe pancreatitis induced by tamoxifen treatment in patients who have undergone breast cancer surgery.

Case Presentation: We studied two cases of breast cancer in whom severe acute pancreatitis developed after taking tamoxifen for endocrine therapy in our hospital. A brief literature review was provided to analyze the causes, clinical manifestations, treatment process, and prognosis of severe acute pancreatitis.

Somatic nuclear blebbing in Caenorhabditis elegans is not a feature of organismal aging but a potential indicator of germline proliferation in early adulthood.

Abnormal nuclear morphology is suggested to be a hallmark of aging and one such abnormality is nuclear blebbing. However, little is known about whether and how nuclear blebbing participates in animal aging, and what regulates it. In this study, we show that the frequency of nuclear blebbing in the hypodermis increases during aging in wild-type C.

Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification.

The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost is critical for the biodegradation of PET in industry. In the present study, we demonstrate that the post-translational glycosylation of Pichia pastoris makes it a remarkable host for the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase). Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P.

Fusion and expansion of vitellogenin vesicles during Caenorhabditis elegans intestinal senescence.

Some of the most conspicuous aging phenotypes of C. elegans are related to post-reproductive production of vitellogenins (Vtg), which form yolk protein (YP) complexes after processing and lipid loading. Vtg/YP levels show huge increases with age, and inhibition of this extends lifespan, but how subcellular and organism-wide distribution of these proteins changes with age has not been systematically explored.

Qualitative and Quantitative Real-Time PCR Methods for Assessing False-Positive Rates in Genetically Modified Organisms Based on the Microbial-Infection-Linked Gene.

The hygromycin phosphotransferase () gene as a selective marker is normally used in screening tests as a first step in detecting and quantifying genetically modified organisms (GMOs) in seeds, food, and feed materials. Nevertheless, if researchers only focus on the gene, it is difficult to distinguish genetically modified (GM) crops from microbial infection, leading to miscalculation of the rate of GM materials in a given sample set. Here, we cloned the 7259 bp sequence carrying the gene from soybean sprouts using the genome walking strategy.

Circulating Tumor-Derived Endothelial Cells: An Effective Biomarker for Breast Cancer Screening and Prognosis Prediction.

Background: Circulating tumor-derived endothelial cell (CTEC) is a new potential tumor biomarker to be associated with cancer development and treatment efficacy. However, few evidences are available for breast cancer.

Methods: Eighty-nine breast cancer patients were recruited, and preoperative and postoperative blood samples were collected.