Exploring the Mechanisms of Amino Acid and Bioactive Constituent Formation During Fruiting Body Development in by Metabolomic and Transcriptomic Analyses.

, a common edible mushroom, is prized for its exceptional taste and rich nutritional composition. The concentrations of amino acids and polysaccharides in the fruiting body exhibited a dynamic increase throughout development, reaching their highest levels in the maturation stages, with values of 45,107.39 μg/g and 13.

Non-coding RNA mediates the defense-associated reverse transcriptase (DRT) anti-phage oligomerization transition.

Defense-associated reverse transcriptase (DRT) systems are implicated in prokaryotic resistance to viral infections, yet the molecular mechanisms underlying their functionality remain largely unknown. Here, we characterize a two-component DRT9 system, composed of a reverse transcriptase (RT) and a non-coding RNA (ncRNA), which exhibits a protein-primed DNA synthesis activity upon phage infection. We also determine its cryo-electron microscopy (cryo-EM) structures in different functional states.

Naphazoline eye drops for the treatment of Horner syndrome following radical thyroid cancer surgery: A case report.

Horner syndrome is a rare complication following thyroid surgery, typically presenting with ptosis, miosis, and ocular congestion. We report the case of a 30-year-old female diagnosed with papillary thyroid carcinoma who underwent left thyroid lobectomy, isthmectomy, and comprehensive left cervical lymph node dissection. On the first postoperative day, the patient developed left-sided ptosis, a constricted pupil, blurred vision, and conjunctival congestion, leading to a clinical diagnosis of Horner syndrome.

Impact of expanded Thrombolysis in Cerebral Infarction (eTICI) grades on clinical outcomes in patients with large ischemic strokes receiving endovascular treatment.

Objective: This study aimed to investigate the impact of the expanded Thrombolysis in Cerebral Infarction (eTICI) grades on clinical outcomes after endovascular treatment (EVT) for large ischemic strokes.

Methods: This study was a substudy of a prospective nationwide multicenter registry that included 490 patients with large ischemic strokes undergoing EVT. The primary outcome was a 90-day modified Rankin Scale (mRS) score of 0-3.

A Clustered Adaptive Exposure Time Selection Methodology for HDR Structured Light 3D Reconstruction.

Fringe projection profilometry (FPP) has been widely applied in industrial 3D measurement due to its high precision and non-contact advantages. However, FPP often encounters measurement problems with high-dynamic-range objects, consequently impacting phase computation. In this paper, an adaptive exposure time selection method is proposed to calculate the optimal number of exposures and exposure time by using an improved clustering method to divide the region with different reflection degrees.

A breathless brake on ferroptosis.

In this issue of Molecular Cell, Minikes et al. reveal that hypoxia suppresses ferroptosis independently of HIF signaling via inhibition of the oxygen-sensitive histone demethylase KDM6A, linking hypoxia-mediated chromatin regulation to phospholipid metabolism and ferroptosis resistance in cancer.

Breaking age barriers: the efficacy of ERCP in advanced age patients.

Background: This study aims to evaluate the efficacy and safety of therapeutic endoscopic retrograde cholangiopancreatography (ERCP) in patients aged 75 years and older.

Methods: A total of 723 patients who underwent ERCP at Anhui Provincial Corps Hospital of the Chinese People's Armed Police Force between March 2018 and April 2022 were retrospectively analyzed. This cohort included 152 patients aged ≥ 75 years (elderly group) and 571 patients aged < 75 years (control group).

Finotonlimab (PD-1 inhibitor) plus bevacizumab (bevacizumab biosimilar) as first-tier therapy for late-stage hepatocellular carcinoma: a randomized phase 2/3 trial.

We aimed to assess the tolerability and efficacy of finotonlimab (an anti-programmed cell death protein-1 antibody) in combination with SCT510, a bevacizumab biosimilar, versus sorafenib in unresectable advanced HCC. This randomized phase 2 and 3 study (ClinicalTrials.gov, NCT04560894; Chinadrugtrials.

SLFN11: a pan-cancer biomarker for DNA-targeted drugs sensitivity and therapeutic strategy guidance.

Therapeutic responses to identical chemotherapy regimens often vary significantly among patients with the same type of cancer, underscoring the need for additional biomarkers to identify individuals most likely to benefit from specific treatments. The expression of SLFN11 (Schlafen11) has been identified as a potential biomarker for predicting patient responses to DNA-damaging agents and PARP inhibitors, as it irreversibly blocks DNA replication under replication stress, thereby increasing the sensitivity of cancer cells to various DNA-damaging agents and PARP inhibitors. Preclinical and clinical trial data suggest that SLFN11 can predict therapeutic responses to multiple DNA- targeted drugs, including platinum-based agents, topoisomerase I/II inhibitors, DNA synthesis inhibitors, and PARP inhibitors.

CTHRC1 Derived From Cancer-Associated Fibroblasts Promotes Pancreatic Cancer Progression and Metastasis via the LIF-STAT3 Pathway.

Background: Collagen triple helix repeat containing 1 (CTHRC1) is a secreted protein involved in tissue remodeling and fibrotic processes, which also suggests emerging roles in cancer. Studies have shown that it is mainly expressed in the outer membrane fibroblasts of injured arteries and in the neointimal smooth muscle cells, where it promotes cell migration and tissue damage repair. However, the regulatory role of CTHRC1 as a tumor microenvironment factor in pancreatic cancer is not well understood.

Research and Clinical Progress of Therapeutic Tumor Vaccines.

Therapeutic cancer vaccines are a new growth point of biomedicine with broad industrial prospects in the post-COVID-19 era. Many large international pharmaceutical companies and emerging biotechnology companies are deploying different tumor therapeutic cancer vaccine projects, focusing on promoting their clinical transformation, and the vaccine industry has strong momentum for development. Such vaccines are also the core engine and pilot site for the development of new vaccine targets, new vectors, new adjuvants, and new technologies, which play a key role in promoting the innovation and development of vaccines.

Development and validation of a novel prognostic model to predict 1-year post-transplant mortality for acute-on-chronic hepatitis B liver failure: a nationwide, multicentre, cohort study.

Background: Liver transplantation (LT) provides a potential cure for hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF). We aimed to develop and externally validate a prognostic model to predict 1-year post-LT mortality in patients with HBV-ACLF.

Methods: This retrospective, nationwide, observational cohort study was conducted at ten high-volume LT centres in China.

Analysis of Composition, Structure, and Driving Factors of Root-Associated Endophytic Bacterial Communities of the Chinese Medicinal Herb .

The role of endophytic bacteria in the interaction between medicinal plants and microorganisms, secondary metabolite accumulation, plant nutrient changes, as well as their interactions with microbial communities, needs to be investigated in medicinal plants. In this study, 16S rRNA genes of endophytic bacterial communities in the root systems of three medicinal licorice species at different root depths (0-20, 20-40, and 40-60 cm) were sequenced using high-throughput sequencing technology, and their relationships with plant and soil factors were investigated. Our study indicated that the influence of species on the structure of endophytic bacterial communities is significantly greater than that of root depth, and there are significant differences in the structure of endophytic bacterial communities at different sampling sites.

A ratiometric fluorescent probe for the detection of fluoroquinolones and tetracyclines.

Antibiotics are widely used globally, leading to significant environmental issues and food contamination. This has sparked interest in developing methods to detect various antibiotics. In this study, we synthesized blue fluorescent carbon dots (g-CDs) using a microwave heating technique.

Heat-responsive ame-miR-1-3p modulates thermotolerance in honeybees (Apis mellifera L.).

Thermal stress significantly harms the survival, physiology and development of honeybees (Apis mellifera L.). miRNAs are emerging as key regulators of environmental stress responses.

Process optimization and flavor characteristics of cream cheese fermented with avocado co-cultures.

This study investigated the impact of avocado co-fermentation on the texture, microstructure, and flavor profile of cream cheese. Avocado puree served as an substrate for R704, yielding a viable cell count of 187.67 × 10 CFU/g after fermentation.

Novel polycyclic meroterpenoids with protein tyrosine phosphatase 1B inhibitory activity isolated from desert-derived fungi Talaromyces sp. HMT-8.

Seven previously undescribed polycyclic meroterpenoids talarines K-Q (1-7), along with five known ones (8-12), were isolated from desert-derived fungi Talaromyces sp. HMT-8. The structure of the novel compounds were elucidated using spectroscopic methods, including electronic circular dichroism (ECD), HRESIMS and nuclear magnetic resonance (NMR) spectroscopy.

LCwmcaR: Learning Cross-Window Cross-Modality Correlation-Aware Representation for Human Activity Recognition.

Deep learning (DL)-based human activity recognition (HAR) has attracted considerable attention owing to its vast potential across various applications. Currently, HAR still faces two challenges. For one thing, existing methods neglect the spatial distribution information embedded in HAR signals and lack the ability to comprehensively model the spatial-temporal (ST) dependencies within HAR data, restricting them from effectively decoding human activity.

Differential effects of inulin and fructooligosaccharides on gut microbiota composition and glycemic metabolism in overweight/obese and healthy individuals: a randomized, double-blind clinical trial.

Background: Modulating the gut microbiota with prebiotics is a promising strategy for managing metabolic diseases. However, the clinical effects on glycemic metabolism across different populations remain uncertain. In this study, we conducted a randomized, double-blind investigation to examine the impact of inulin and fructooligosaccharides (FOS) on glycemic metabolism in overweight/obese and healthy adults.

Prognostic impact of tace combined with sorafenib or lenvatinib followed by regorafenib in recurrent hepatocellular carcinoma after liver transplantation: a retrospective study.

Background: Tumor recurrence after liver transplantation (LT) for hepatocellular carcinoma (HCC) remains a significant challenge. This study systematically evaluates the efficacy and safety of transarterial chemoembolization (TACE) combined with tyrosine kinase inhibitors (TKIs) for post-LT tumor recurrence.

Methods: This retrospective study included 78 patients with recurrent intrahepatic HCC after LT.

Correction: Research on distribution network fault processing technology based on knowledge of graph.

[This corrects the article DOI: 10.1371/journal.pone.

[Retracted] Everolimus inhibits the proliferation and migration of epidermal growth factor receptor-resistant lung cancer cells A549 via regulating the microRNA-4328/phosphatase and tensin homolog signaling pathway.

[This retracts the article DOI: 10.3892/ol.2019.

Adjuvant chemotherapy improves post-transplant outcome in patients with hepatocellular carcinoma.

Background: Hepatocellular carcinoma (HCC) recurrence following liver transplantation (LT) remains a major challenge. This study aimed to investigate the effect of adjuvant chemotherapy (ACT) with the modified FOLFOX-6 (mFOLFOX-6) regimen on the post-transplant prognosis of HCC patients.

Methods: HCC patients who underwent LT at our institution from June 2017 to December 2019 were enrolled.