Observation of Giardia duodenalis infection in Mongolian gerbils: Cysts isolated from bovine feces.

Assemblage E of Giardia duodenalis, primarily infecting ruminants, has been relatively understudied both in vivo and in vitro. Due to unsuccessful attempts at in vitro cultivation, this study focused on establishing an economical, stable, and clinically relevant experimental animal model for Assemblage E infections. Cysts were purified from bovine feces via 33 % zinc sulfate flotation, with Assemblage E identity confirmed by gdh gene sequencing.

Consensus statement on the multidisciplinary treatment of nasopharyngeal carcinoma with immune checkpoint inhibitors in China.

Introduction: This study aims to establish a multidisciplinary consensus on the treatment of nasopharyngeal carcinoma (NPC) in China, with a particular focus on the guidelines for the use of immune checkpoint inhibitors (ICIs) to ensure scientific and rational treatment protocols.

Methods: The consensus was formulated based on the 'guidelines for the diagnosis and treatment of NPC (2024 edition)' issued by the Chinese Society of Clinical Oncology. The Delphi method was used to collect and consolidate expert opinions.

One-pot preparation of Au-Pt bimetallic single-atom nanozymes with efficient peroxidase-like activity using chitosan and l-tyrosine as carriers for visual and colorimetric detection of quercetin.

An one-pot method was used to prepare bimetallic nanozymes, with chitosan (CS) and l-tyrosine (L-Tyr) as stabilized dispersed colloidal solutions and a carrier for gold-platinum single atoms (Au-Pt SAs), which exhibited excellent peroxidase activity. A colorimetric method based on CS/L-Tyr/Au-Pt SAs nanozymes was constructed for the colorimetric detection of quercetin (QR) in human serum and orange juice. The synthesized bimetallic nanozymes were characterized by SEM, TEM, HAADF-STEM, FT-IR, XRD and XPS techniques to demonstrate the successful synthesis of CS/L-Tyr/Au-Pt SAs nanozymes.

BHLHE40 Cooperates with GATA2/3 to Control Human Syncytiotrophoblast Lineage Differentiation.

Syncytiotrophoblasts (STBs) constitute one of the core components of the placenta, responsible for synthesizing pregnancy-sustaining hormones such as human chorionic gonadotropin (HCG). Deficient syncytialization of cytotrophoblasts affects the hormonal secretion and placental development, contributing to pregnancy-associated disorders, including spontaneous miscarriage. To date, the molecular mechanisms, particularly the role of transcription factors (TFs), in STB lineage specification remain incompletely understood.

Application of nanobody‑based CAR‑T in tumor immunotherapy (Review).

Chimeric antigen receptor (CAR) T cell therapy is a type of cellular immunotherapy showing promising clinical effectiveness and high precision. CAR‑T cells express membrane receptors with high specificity, which enable them to identify certain target antigens generated by cancerous cells. The three primary structural elements of the CAR are the extracellular domain, transmembrane domain and cytoplasmic domain.

Role of monocytes in the pathogenesis of antiphospholipid syndrome and potential therapeutic targets (Review).

Antiphospholipid syndrome (APS) is an autoimmune disorder characterized primarily by arterial and/or venous thrombosis, obstetric complications and persistent positivity for antiphospholipid antibodies (aPLs). It has been proposed that the pathogenesis of APS is closely associated with vascular endothelial cell activation, complement activation and platelet activation. Notably, APS may be key to understanding the relationship between innate immune cells, and thrombosis and obstetric complications.

Indobufen Versus Aspirin Plus Clopidogrel in Patients After Coronary Stenting in Patients With Diabetes: A Post Hoc Analysis of the OPTION Trial.

Background: Despite increased risk of ischemic events in diabetes, the optimal anti-thrombotic strategy for secondary prevention has not been defined. We aimed to assess the efficacy and safety of optimal antiplatelet agents such as indobufen-based dual antiplatelet therapy (DAPT) in patients with diabetes after coronary stenting.

Methods: OPTION trial was a randomized, open-label, noninferiority, and multicentric study in China.

A bidirectional interfacial engineering strategy for highly stable sodium metal batteries.

Sodium (Na) metal batteries (SMBs) are regarded as some of the most promising next-generation energy storage systems due to their high energy density. However, their practical application is severely hindered by interfacial instabilities at both the anode and cathode, which result in rapid capacity degradation during cycling. Here, we proposed a bidirectional interfacial regulation strategy that simultaneously stabilizes both electrode interfaces.

Who determines nasopharyngeal carcinoma inpatients' medical expenditure? Importance factor ranking using the random forest model.

Background: Nasopharyngeal carcinoma (NPC) is a malignant epithelial tumor most commonly in China. In 2013, NPC incidence and mortality in China were also at high levels worldwide, which poses a great health burden in China. This study analyzes the medical expenditure and influencing factors of inpatients with NPC and aims to provide reference suggestions for reducing medical expenditure for NPC.

Clinical manifestations and outcomes of HSK following corneal transplantation.

Purpose: To investigate the clinical manifestations and outcomes of herpes simplex keratitis (HSK) infection following corneal transplantation.

Methods: This retrospective study analyzed medical records of patients who underwent corneal transplantation at the People's Hospital of Guangxi Zhuang Autonomous Region between January 2018 and March 2024, with a minimum follow-up period of 1 year. The study examined post-transplantation herpes simplex virus (HSV) infections, including the timing of HSV infection, HSK classification, clinical manifestations, and outcomes.

SFB flagellin mediates cell adhesion, endocytosis and immune regulation in germ-free mice.

Introduction: Segmented filamentous bacteria (SFB) colonization dynamics are crucial for host immune regulation. Given this, the present study specifically examined the functions of SFB flagellin in bacterial adhesion, cellular internalization, and immune modulation.

Methods: and were engineered to express murine and rat SFB flagellin genes.

Electrochemistry-Accelerated Water-Gas Shift Reaction on IrN-RhN Dual Sites Catalysts for Efficient Hydrogen Production.

The water-gas shift reaction (WGSR) is crucial to the hydrogen economy, which is hampered by the harsh conditions and complicated purification process. In this work, the spatially separated efficient CO conversion and high-purity H production are realized by electrochemistry-accelerated water-gas shift reaction (WGSR) with IrN-RhN dual sites single atom catalysts (IrRh-NC) in high-temperature polymer-electrolyte-membrane electrolyzer. In this reaction, the Ir single atoms in the catalysts can rapidly dissociate HO at an extremely low potential to supply abundant *OH, which ensures the *OH groups bind to the spontaneously adsorbed *CO on neighboring Rh sites to further accelerate CO conversion.

CTRP3 mitigates sepsis-induced impairment of endothelial barrier function by suppressing pyroptosis in endothelial cells.

Acute lung injury significantly contributes to mortality in bacterial sepsis due to lung endothelial barrier destruction, leading to protein-rich lung edema, an influx of proinflammatory leukocytes, and persistent hypoxemia. CTRP3, an adipokine, reduces endothelial adhesion molecules Vcam-1 and Icam-1 and inhibits LPS-induced monocytic adhesion, highlighting its anti-inflammatory effects. This study investigates CTRP3's protective role in sepsis-induced acute lung injury, revealing reduced CTRP3 expression during sepsis, which worsens endothelial dysfunction.

ONECUT1 activates PSAT1 to facilitate PD-L1 expression and mediate immune evasion of lung squamous cell carcinoma.

Background: PD-1/PD-L1, a classic immune checkpoint commonly employed in targeted therapy, has proven to yield only limited benefits for patients with lung squamous cell carcinoma (LUSC). Unraveling the intrinsic mechanisms underlying the progression of LUSC serves as the foundation for discovering more effective treatment strategies.

Methods: A study was conducted on the differential expression of PSAT1 and ONECUT1 in LUSC based on data from the TCGA database.

Taurine Supplementation Enhances the Resistance of Postlarvae to Low-Salinity Stress.

In aquaculture, Pacific white shrimp () growth in low-salinity waters is limited by osmoregulatory stress; therefore, improving resistance to low-salinity stress via nutritional modulation is key. In the present study, shrimp postlarvae were provided with a taurine supplement under low-salinity stress, and then the survival rate, the histology, the Na/K-ATPase (NKA) expression pattern and transcriptomic sequencing were investigated to evaluate the postlarval responses. The results showed that the postlarva survival rate in low-salinity water was only 61.

Aquaculture Strategy and Genetic Diversity of in Beibu Gulf, China.

has become one of the pillar industries in the aquaculture of the Beibu Gulf since it was introduced into China in 1991. This study examined how stocking density and culture site affects growth in breeding populations, compared their growth performance and genetic diversity within control populations, and identified optimal culture locations for in the Beibu Gulf. The environmental investigation results revealed that among the three aquaculture sites of Beihai (BH), Qinzhou (QZ) and Fangchenggang (FCG), the fluctuation ranges of salinity, pH, and dissolved oxygen at the BH site were relatively narrower.

Effects of Dietary on Muscle Composition, Serum Biochemical Indicators, Antioxidant Capacity, Lipid Metabolism, and mTOR Signaling Pathway in Red Claw Crayfish ().

This study investigated the effects of dietary supplementation with varying levels (CK: 0.0 g/kg; RL: 0.1 g/kg; RM: 1.

Engineered Exosomes Complexed with Botulinum Toxin Type A for Enhanced Anti-Aging Effects on Skin.

Skin aging is commonly characterized by increased wrinkles, loss of elasticity, and hyperpigmentation, significantly affecting personal appearance and quality of life. Although botulinum toxin type A (BTX-A) has been widely applied in cosmetic anti-wrinkle treatments, its intrinsic cytotoxicity limits broader clinical applications. In this study, we developed a novel exosome-based BTX-A composite delivery system designed to synergize the anti-aging properties of exosomes with the wrinkle-reducing effects of BTX-A while reducing toxicity.

Hydrogen-Rich Water Attenuates Diarrhea in Weaned Piglets via Oxidative Stress Alleviation.

Early weaning of piglets elicits weaning stress, which in turn induces oxidative stress and consequently impairs growth and development. Hydrogen-rich water (HRW), characterized by selective antioxidant properties, mitigates oxidative stress damage and serves as an ideal intervention. This study aimed to evaluate the effects of HRW on weaned piglets, specifically investigating its impact on growth performance, diarrhea incidence, antioxidant function, intestinal morphology, gut microbiota, and hepatic metabolites.

Population Genetics of the Asian Buffalo Leech () in Southern China Based on Mitochondrial Protein-Coding Genes.

Leeches hold significant medical and pharmaceutical value for antithrombotic treatments, yet their genetic diversity patterns remain poorly understood. We performed population genetic analyses on seven populations from southern China using mitochondrial protein-coding genes (MitPCGs). Complete sequences of all 13 MitPCGs were obtained from 74 individuals.

ComplexDnet: A Network-Based Strategy to Discover Critical Targets and Screen Active Compounds for Complex Diseases.

The etiology of complex diseases such as metabolic-associated steatohepatitis (MASH) presents significant challenges for therapeutic discovery. Here, we developed ComplexDnet, a transcriptome- and network-integrated framework to prioritize disease-relevant targets. Applied across eight cancer types, ComplexDnet achieved an average recall of 77.

Microvesicle-transferred mitochondria trigger cGAS-STING and reprogram metabolism of macrophages in sepsis.

The inflammatory cytokine storm is a hallmark of sepsis and is highly correlated with organ injury. Therefore, inhibiting inflammatory cytokine production is a straightforward strategy for effectively treating this disease. In this study, we found that microvesicles from lipopolysaccharide (LPS)-primed macrophages could transfer mitochondria to other macrophages and alter their biological functions.

Versatile Applications of CRISPR-Based Programmable T-DNA Integration in Plants.

Agrobacterium-mediated T-DNA integration into plant genomes represents a cornerstone for transgenic expression in plant basic research and synthetic biology. However, random T-DNA integration can disrupt essential endogenous genes or compromise transgene expression, stressing the need for targeted integration strategies. Here we explored CRISPR-aided targeted T-DNA integration (CRISTTIN) in Arabidopsis, leveraging CRISPR-induced double-strand breaks (DSBs) to facilitate precise T-DNA insertion.

Exogenous jasmonic acid and salicylic acid enhance selenium uptake and mitigate cadmium accumulation in pak choi ( L.) grown in selenium-rich, high-cadmium soil.

Plant hormones are known to regulate the uptake and distribution of mineral elements, including heavy metals, in crops. This study evaluated the effects of exogenous jasmonic acid (JA) and salicylic acid (SA), applied individually or in combination, on selenium (Se) enrichment and cadmium (Cd) mitigation in pak choi ( L.) cultivated in Se-rich and high-Cd soils.

Association between advanced lung cancer inflammation index and distant metastasis in breast cancer: a retrospective cohort study.

Objective: This study aims to assess the relationship between the Advanced Lung Cancer Inflammation Index (ALI) and the risk of distant metastasis in breast cancer. While ALI is commonly used to evaluate the prognosis of lung cancer patients, its application in breast cancer and its correlation with distant metastasis are not well understood. Therefore, this study explores the potential of ALI as a predictor of distant metastasis in breast cancer patients.