SFB flagellin mediates cell adhesion, endocytosis and immune regulation in germ-free mice.

Introduction: Segmented filamentous bacteria (SFB) colonization dynamics are crucial for host immune regulation. Given this, the present study specifically examined the functions of SFB flagellin in bacterial adhesion, cellular internalization, and immune modulation.

Methods: and were engineered to express murine and rat SFB flagellin genes.

Transcriptomic, phenotypic and physiological analyses identify candidate regulators of Dendrobium stigma maturation and pollen-stigma interaction.

Background: species are prized for their ornamental and economic value, yet their low hybrid compatibility remains a critical challenge, potentially linked to stigma recognition mechanisms. The stigma, as the primary site for pollen interaction, undergoes dynamic developmental changes that lay the foundation for successful pollination. However, the molecular mechanisms governing stigma maturation and subsequent pollen recognition are poorly characterised.

Oral microbiome components predict response to neoadjuvant chemotherapy in triple-negative breast cancer patients.

The oral microbiome has emerged as a critical biomarker and regulator in cancer development and treatment response, garnering increasing attention from researchers. However, its specific role in breast cancer, particularly in triple-negative breast cancer (TNBC), remains poorly understood. The influence of the oral microbiome on chemotherapy sensitivity in TNBC, along with the underlying molecular mechanisms, remains unclear.

7-step endoscopic nipple-sparing mastectomy with implant-based breast reconstruction: nipple sensation preservation and low complications.

Background: Single-port endoscopic nipple-sparing mastectomy (E-NSM) with implant-based breast reconstruction (IBBR) represents a promising surgical technique. However, the surgical procedures are complex and lack standardized and systematic surgical protocols. The seven-step method was introduced as a means of simplifying the complex surgical procedures, with detailed descriptions provided of the key points and critical aspects of the surgery.

Artificial Intelligence-powered copilots for precision diagnosis and surgical assessment of histological growth patterns in resectable colorectal liver metastases: a prospective study.

Background: Colorectal cancer (CRC) is a leading cause of mortality in China, with metastasis significantly contributing to poor outcomes. Histopathological growth patterns (HGPs) in colorectal liver metastasis (CRLM) provide vital prognostic insights, yet the limited number of pathologists highlights the need for auxiliary diagnostic tools. Recent advancements in artificial intelligence (AI) have demonstrated potential in enhancing diagnostic precision, prompting the development of specialized AI models like COFFEE to improve the classification and management of HGPs in CRLM patients.

High-Efficiency Flexible Zn-Air Batteries Enabled by Agarose Based Oxygen Electrocatalyst and Gel Electrolyte Through Bidirectionally Synergistic Optimization Strategy.

Flexible Zn-air batteries (F-ZABs) typically suffer from limited cycle life due to sluggish oxygen electrocatalytic kinetics and unstable electrochemical interfaces. A bidirectionally synergistic strategy for profit is proposed from the unique elemental characteristics and molecular architecture of agarose to simultaneously construct a triple-doped N, P, O oxygen electrocatalyst with multiple active sites and gel electrolyte with exceptional mechanical robustness and weather resistance. The electrocatalyst demonstrates superior oxygen reduction reaction (ORR) activity (E = 0.

Robot assisted sentinel lymph node biopsy using indocyanine green combined with carbon nanoparticles staining improved detection rates in breast cancer.

This study aimed to evaluate the safety and feasibility of robot-assisted sentinel lymph node biopsy using carbon nanoparticles with or without indocyanine green in breast cancer. A total of 52 patients with breast cancer who underwent robot-assisted sentinel lymph node (SLN) biopsy and subcutaneous mastectomy between August 2023 and September 2024 were extracted. Data collection included patient demographics, metastasis status, and surgery parameters.

The effect of Zuogui-Jiangtang-Yishen decoction on the intestinal flora's response to L-α-phosphatidylcholine and L-tyrosine in patients with diabetic kidney disease: an study.

Background: Animal and cell studies have demonstrated that Zuogui-Jiangtang-Yishen decoction (ZGJTYS) has a favorable effect on the treatment of diabetic kidney disease (DKD). Our previous clinical research also showed that ZGJTYS prevents DKD in a manner similar to that of benazepril. Nevertheless, the interactions between ZGJTYS and the human gut microbiota require further investigation, particularly its interference in the intestinal flora response to food ingredients that may increase DKD risk, such as L-α-phosphatidylcholine and L-tyrosine.

Minimally invasive axillary lymph node dissection versus conventional axillary lymph node dissection: a comparative study on short-term efficacy in breast cancer.

Background: This study aims to compare the short-term clinical efficacy of minimally invasive axillary lymph node dissection (MIAD) versus conventional axillary lymph node dissection (CAD) in the treatment of breast cancer with positive axillary lymph nodes.

Methods: We performed a retrospective analysis of the clinical data of breast cancer patients who underwent MIAD and CAD in the Breast Surgery Department from January 2022 to August 2024. The primary outcomes assessed included surgical results, complications, post-operative upper limb function, and quality of life score for both groups.

Efficacy and safety of robotic-assisted versus endoscopic-assisted axillary lymph node dissection in node-positive breast cancer: a retrospective comparative study.

Background: Robotic surgery is increasingly being adopted for breast cancer treatment. However, robust clinical evidence regarding its effectiveness and safety remains limited. This retrospective cohort study aimed to compare the surgical quality and short-term outcomes of robotic-assisted axillary lymph node dissection (R-ALND) and endoscopic-assisted axillary lymph node dissection (E-ALND) in patients with node-positive breast cancer.

Genetic Diversity and Growth-Promoting Functions of Endophytic Nitrogen-Fixing Bacteria in Apple.

Understanding the dominant populations and biological functions of endophytic nitrogen-fixing bacteria in apple plants is of great significance for the healthy growth management and sustainable development of apple cultivation. In this study, we investigated the community diversity and potential plant growth-promoting abilities of endophytic nitrogen-fixing bacteria in different tissues of apple trees by combining high-throughput sequencing of the gene with traditional isolation and cultivation techniques. Sequencing results revealed that the endophytic bacteria were affiliated with 10 phyla, 14 classes, 30 orders, 42 families, and 72 genera.

The application of methylene blue location technique in deep-seated benign breast tumor resection under endoscopy: a retrospective, single-institution analysis.

Background: Endoscopic curative excision of benign breast diseases (BBDs) can preserve the cosmetic appearance of the breast. However, endoscopic surgery is not feasible, and some challenges still need to be addressed. Traditional line marker localization methods cannot visualize tumors, and the exploration of deep tumors may lead to certain risks of accidental injury.

Clinical effectiveness of transaxillary single-port endoscopic surgery in the treatment of breast benign tumor.

Background: Benign breast lumps affect 10% of women in their lifetimes. Endoscopic surgery could be an alternative surgical technique for benign breast tumors because it is performed through small wounds hidden in inconspicuous areas. The aim of this study was to explore the safety and esthetic effects of endoscopic surgery in the treatment of benign breast disease.

Sun-Cross Running Suture Combined with Transverse to Longitudinal Skin Closure to Correct Severe Inverted Nipple.

Background: Inverted nipple deformity presents an unsatisfactory appearance that may induce an unpleasant sex life, but can also be associated with psychological discomfort and increased the functional problems, such as local irritation and inflammation.

Methods: Multiple techniques have been used to correct inverted nipples, but they mostly lead to different problems such as deficiency of the nerve or duct, recurrence of the inverted nipple, and hypopigmented scars in the areola. To minimize complications and maintain the stability of the reconstructed nipple, we presented a minimal incision technique that designed four 3-mm-sized horizontal microincisions, which ran a sun-cross through the periphery and the core of the nipple to push the nipple together, then a vertical suture ran longitudinal to close the transverse incision to stabilize the projection.

Whole-genome sequencing of and Sanguinarine-induced mutant.

This work was performed on commercially purchased CVCC519 originally isolated from chicken intestinal content. The Sanguinarine-resistant strain XM3104 was isolated from Sanguinarine-induced CVCC519. To identify possible mechanisms underlying resistance, the complete genomes of CVCC519 and XM3104 were sequenced using PromethION and next-generation sequencing.

Circ_0020256 induces fibroblast activation to drive cholangiocarcinoma development via recruitment of EIF4A3 protein to stabilize KLF4 mRNA.

Cancer-associated fibroblasts (CAFs) are a kind of stromal cells in the cholangiocarcinoma (CCA) microenvironment, playing crucial roles in cancer development. However, the potential mechanisms of the interaction between CCA cells and CAFs remain obscure. This work investigated the role of circ_0020256 in CAFs activation.

EIF4A3-induced circZFAND6 promotes breast cancer proliferation and metastasis through the miR-647/FASN axis.

Aims: Circular RNAs (circRNAs) are important regulators in breast cancer progression. However, the underlying mechanism of circRNAs functions in breast cancer remain largely unclear.

Main Methods: To investigate the circRNAs expression pattern in breast cancer, high-throughput circRNA microarray assay was used.

Whole-Genome Sequencing of Pig Gut Lactobacillus amylovorus CICC 6090.

Lactobacillus amylovorus has been reported to reduce weight and fat content in humans. To identify and understand the mechanism underlying this process, the complete genome of CICC 6090, which was isolated from pig intestines, was sequenced using PromethION and next-generation sequencing.

SHH/GLI2-TGF-β1 feedback loop between cancer cells and tumor-associated macrophages maintains epithelial-mesenchymal transition and endoplasmic reticulum homeostasis in cholangiocarcinoma.

Background: Tumor-associated macrophages (TAMs) play a dual role in tumors. However, the factors which drive the function of TAMs in cholangiocarcinoma remain largely undefined.

Methods: SHH signaling pathway and endoplasmic reticulum stress (ERS) indicators were detected in clinical tissues and cholangiocarcinoma cell lines.

New Bounds on Capacity Region of Multiple Access Channels in Visible Light Communications.

In this paper, we propose new inner and outer bounds of the capacity region for multiple access channels in visible light communication (VLC) networks under both peak and average optical power constraints. Specifically, the proposed inner bounds are established by employing the single-user capacity achieving input distribution for each user. The proposed outer bounds are derived by determining single-user capacities for each user and calculating a sum capacity upper bound by relaxing the input constraints.

Step-by-Step Method Combined with Simulation-Enhanced Learner Outcomes in Minimally Invasive Breast Surgery Education.

Purpose: Traditional standardized training in ultrasound-guided minimally invasive breast surgery (UMIBS) focused on lecture-based learning (LBL) resulted in students' insufficient analysis, limited spatial visual conversion ability, and poor practical application. This study examined the effects of the step-by-step (SBS) method combined with a simulation model in UMIBS education.

Subjects And Methods: A total of 84 residents participated in this study.

Long-term efficacy of percutaneous transhepatic cholangioscopy-guided photodynamic therapy for postoperative recurrent extrahepatic cholangiocarcinoma.

Background: Cholangiocarcinoma recurs frequently following excision surgery and is usually inoperable, while radiotherapy, chemotherapy, and immunotherapy are of limited benefit. As palliative care, percutaneous transhepatic cholangial drainage (PTCD) can relieve biliary obstruction, prevent jaundice, and maintain quality of life (QOL), but does not improve overall survival. In contrast, photodynamic therapy (PDT) has been demonstrated to prolong the survival of inoperable cancer patients.

Long Non-Coding RNA LINC00052 Targets miR-548p/Notch2/Pyk2 to Modulate Tumor Budding and Metastasis of Human Breast Cancer.

Abnormal expression of long non-coding RNAs (lncRNAs) is involved in many pathological processes of cancers. However, the role of lncRNA LINC00052 in breast cancer progression is still unclear. Here, LINC00052 expression was detected by in situ hybridization and quantitative real-time PCR assays.