ComplexDnet: A Network-Based Strategy to Discover Critical Targets and Screen Active Compounds for Complex Diseases.

The etiology of complex diseases such as metabolic-associated steatohepatitis (MASH) presents significant challenges for therapeutic discovery. Here, we developed ComplexDnet, a transcriptome- and network-integrated framework to prioritize disease-relevant targets. Applied across eight cancer types, ComplexDnet achieved an average recall of 77.

Humidity-sensitive irreversible phase transformation of open-framework zinc phosphate and its water-assisted high proton conduction properties.

Open-framework zinc phosphate (NMe4)(ZnP2O8H3) undergoes irreversible phase transformation. Structural transformation with α (NMe4·Zn[HPO4][H2PO4] the low-temperature phase) and β (NMe4·ZnH3[PO4]2 the high-temperature phase) (Tc = 149 °C) and conduction properties were investigated by single-crystal X-ray diffraction, differential scanning calorimetry, and alternating current (ac) impedance. The open-framework material was sensitive to humidity and β proton conductivity was higher than 10-2 S cm-1 at room temperature and 98% relative humidity (RH).

[Expression of the NMDA receptor in the rat model of orchialgia].

Objective: To explore the expression of the N-methyl-D-aspartate (NMDA) receptor in the rat model of orchialgia and its possible mechanisms.

Methods: According to Yoshioka's method, the male rats in the control group were injected with 0.2 ml saline, and those in the experimental group with 0.

Design and Preparation of a Superior Proton Conductor by Confining Tetraethylenepentamine in the Pores of ZIF-8 To Induce Further Adsorption of Water and Carbon Dioxide.

In this work, we present a new strategy toward the design and preparation of a metal-organic framework- or porous coordination polymer-based superior proton conductor. We chose a robust metal-organic framework, ZIF-8, as the host and a flexible aliphatic alkylpolyamine, tetraethylenepentamine (TEPA), as the guest, and we successfully prepared an encapsulation compound TEPA@ZIF-8 via the facile insertion of TEPA into the pores of ZIF-8, which was characterized by microanalysis, thermogravimetric analysis, IR spectroscopy, N, water vapor adsorption-desorption, and other methods. Each cage in ZIF-8 is occupied by ∼1.

[Role of prostaglandin E receptor EP4 in the regulation of adipogenesis and adipose metabolism].

Adipose tissue is the energy storage organ of the body, and excess energy is stored in adipocytes in the form of lipid droplets. The homeostasis of adipose tissue is the basis for the body to maintain normal metabolic activity. Prostaglandin E (PGE) is an important lipid mediator in the body.

Dimensionality of luminescent coordination polymers of magnesium ions and 1,1'-ethynebenzene-3,3',5,5'-tetracarboxylic acid modulated by structural inducing agents.

Solvothermal reactions of aromatic 1,1'-ethynebenzene-3,3',5,5'-tetracarboxylic acid (HEBTC) and Mg salts in the presence of different supporting ligands afforded the coordination polymers [Mg(HEBTC)(DMF)(HO)] (1), [Mg(HEBTC)(HO)]·solvent (2) and [Mg(EBTC)(HO)]·solvent (3). The crystal structures of 1-3 were determined by the single crystal X-ray diffraction technique, where CP 1 showed a one-dimensional zigzag MgO coordination octahedral chain structure; 2 exhibited a two-dimensional MgO coordination octahedral framework with trinuclear [Mg(COO)] SBUs, and 3 featured a three-dimensional MgO coordination octahedral framework with binuclear [MgO(COO)] SBUs. The various structures in CPs 1-3 of Mg ions with the HEBTC ligand were ascribed to the conformational flexibility and the coordination mode diversity of the HEBTC ligand.

A novel experience of deferential vessel-sparing microsurgical vasoepididymostomy.

Microsurgical longitudinal intussusception vasoepididymostomy (LIVE) has been widely used to treat epididymal obstructive azoospermia since 2004. Although the deferential vasculature plays an important role in supplying blood to the testis and epididymis, little attention has been paid to the potential benefits of sparing the deferential vessels during the anastomosis in LIVE. This study aimed to evaluate the efficacy and safety of deferential vessel-sparing LIVE in humans.

Cryopreservation of virus: a novel biotechnology for long-term preservation of virus in shoot tips.

Background: Preservation of plant virus is a fundamental requirement in all types of virus-related research and applied applications. Development of efficient, reliable strategies for long-term preservation of plant virus would largely assist these studies.

Results: The present study reported a novel biotechnology allowing cryopreservation of Apple stem grooving virus (ASGV) in living shoot tips.

[Expressions of TRPV1 and TRPA1 in the dorsal root ganglion in the rat model of orchialgia].

Objective: To explore the expressions of transient receptor potential vanilloid 1 (TRPV1) and TRP ankyrin 1 (TRPA1) in the dorsal root ganglion (DRG) and their action mechanisms in the rat model of orchialgia.

Methods: The models of orchialgia were established in male SD rats by injection of 2% acetic acid into the testis. Then the number of spontaneous pain responses and withdrawal latency in the model rats were recorded by behavioral tests and the expressions of TRPV1 and TRPA1 in T13－L1 DRGs determined by RT-qPCR, Western blot and immunofluorescence staining.

Subinguinal microsurgical varicocelectomy with intraoperative microvascular Doppler ultrasound leads to the pain-free outcome after surgery.

Objective: To determine efficacy of intraoperative microvascular Doppler ultrasound in assisting subinguinal microsurgical varicocelectomy for pain relief in the treatment of painful varicoceles, compared to the microsurgery without Doppler ultrasound.

Methods: Total 153 patients underwent microsurgical varicocelectomy were randomly allocated to two groups: Groups 1 and 2 included 82 and 71 patients monitored with and without using intraoperative microvascular Doppler ultrasound, respectively. The assessments were compared between two groups, including intraoperative parameters (vessel numbers and operative time) and postoperative outcomes (pain resolution, complications and recurrence).

[Updated diagnosis and management of chronic orchialgia].

Chronic orchialgia (CO) is a common complaint in urology or andrology. Due to its complicated pathogenesis, the diagnosis and treatment of CO are quite challenging. Based on different etiologies, CO can be idiopathic or secondary.

Weight loss correlates with macrophage inhibitory cytokine-1 expression and might influence outcome in patients with advanced esophageal squamous cell carcinoma.

Background: Weight loss during chemotherapy has not been exclusively investigated. Macrophage inhibitory cytokine-1 (MIC-1) might play a role in its etiology. Here, we investigated the prognostic value of weight loss before chemotherapy and its relationship with MIC-1 concentration and its occurrence during chemotherapy in patients with advanced esophageal squamous cell carcinoma (ESCC).

Wild-Type KRAS and BRAF Could Predict Response to Cetuximab in Chinese Colorectal Cancer Patients.

Objective: To analyze the relationship between KRAS, BRAF mutations and the response toCetuximab in Chinese colorectal cancer patients.

Methods: A total of273 Chinese colorectal cancer patients were evaluated for KRAS and BRAF mutations by Sanger sequencing. Among them, 59 patients with metastatic colorectal cancer (mCRC) were treated with Cetuximab in combination with chemotherapy from August 2005 to July 2009.

Thymidine Phosphorylase/β-tubulin III expressions predict the response in Chinese advanced gastric cancer patients receiving first-line capecitabine plus paclitaxel.

Background: To assess the role of Thymidine Phosphorylase and β-tubulin III in clinical outcome of Chinese advanced gastric cancer patients receiving first-line capecitabine plus paclitaxel.

Methods: The clinical data and tumor biopsies prior treatment from 33 advanced gastric cancer patients receiving capecitabine plus paclitaxel (cohort 1, experimental group) and 18 patients receiving capecitabine plus cisplatin (cohort 2, control group) in Beijing Cancer Hospital from July 2003 to December 2008 were retrospectively collected and analyzed for Thymidine Phosphorylase and β-tubulin III expressions by immunohistochemistry. The relationships between expressions of biomarkers and response or survival were determined by statistical analysis.