Molecular Epidemiology and Genomic Insights into Enterobacter cloacae Complex Co-Harboring bla and mcr: Implications for Resistance Gene Transmission.

Objective: This study aims to systematically investigate the molecular epidemiology and genomic characteristics of Enterobacter cloacae complex (ECC) strains globally harboring bla and mcr, as well as the co-existence of drug resistance genes. The goal is to provide insights and recommendations for monitoring clinical drug-resistant strains and super-resistant plasmids.

Methods: This study analyzed 281 ECC isolates harboring both bla and mcr, obtained from NCBI GenBank database (2003-2024) with whole-genome sequencing data.

Macrophage-derived reactive oxygen species promote aggresome formation contributing to bacterial antibiotic persistence.

In this study, we reveal that macrophage-derived reactive oxygen species (ROS) can trigger the rapid formation of aggresomes, which substantially contribute to the increased frequency of persisters induced by phagocytosis. containing aggresomes exhibited a dormant phenotype characterized by reduced adenosine triphosphate (ATP) levels and decreased metabolic activity. Furthermore, these dormant bacteria showed upregulated expression of pathogenicity island 1 (SPI-1)-encoded type III secretion system (T3SS)-related genes, followed by later expression of SPI-2 T3SS-related genes when macrophages ROS production declined.

Genetic characteristics of rotavirus a in Shenzhen and Zhuhai, China, 2020-2023.

Background: Rotavirus A group (RVA) is a leading cause of viral diarrhea, posing a substantial economic and public health burden. Compared to other enteric viruses, RVA possesses diverse genetic mechanisms, making it more challenging to control and prevent. Moreover, surveillance and evolutionary studies on RVA remain limited in Southern China.

The elderly as a population at risk of chronic obstructive pulmonary disease mortality due to ambient ozone exposure: results from the Global Burden of Disease Study 2021.

Background And Objective: To provide guidance on management strategies for chronic obstructive pulmonary disease (COPD), this study systematically analyzed the burden of the disease due to ozone (O) exposure in adults aged ≥ 65 years.

Methods: Data from the Global Burden of Disease Study 2021 were used to analyse trends in the age-standardized mortality rate (ASMR) for O-related COPD in the elderly (≥ 65 years). Age-period-cohort (APC) model was used to analyse independent age, period and cohort effects.

Reduces Susceptibility to Secondary MRSA Infection in IAV-Infected Mice Through Promoting a T Cell-Independent IgA Response.

Secondary methicillin-resistant (MRSA) infection causes high mortality in patients with influenza A virus (IAV). Our previous study observed that the relative abundance of () was significantly reduced in both the respiratory tract and gut of IAV-infected mice and negatively correlated with the severity of IAV-MRSA coinfection pneumonia, but the role of remains unclear. Here, we supplemented the respiratory tract and gut of IAV-infected mice with live and performed a secondary MRSA infection challenge to investigate the effects and potential mechanisms further.

Precise Excited-State Engineering in Benzimidazole Derivatives via Donor-Acceptor Architecture for Organic Temperature-Responsive Phosphorescence.

Herein, we report precise excited-state engineering in benzimidazole (NBd) derivatives via a donor-acceptor (D-A) architecture for organic temperature-responsive phosphorescence (RTP). Four NBd derivatives (1-NBdCN, 1-NBdBrCN, 2-NBdCN, and 2-NBdBrCN) with an electron acceptor at position 1 and position 2 were designed and synthesized. The position of the electron acceptor enables 1-NBdCN and 1-NBdBrCN with temperature-responsive phosphorescence and endows 2-NBdCN and 2-NBdBrCN with red-shifted phosphorescence, showing a remarkable site effect.

Global Evolution and Transmission Dynamics of Enterovirus D68.

Enterovirus D68 (EV-D68), a serotype of the enterovirus species D, has garnered significant attention due to outbreaks reported in 2014, 2016, and 2018. In this study, 36 Chinese EV-D68 strains were isolated, sequenced, and combined with all EV-D68 VP1 sequences from GenBank to form a data set of 1679 sequences. This data set served as the basis for phylogenetic, evolutionary dynamics, phylogeographic, and key amino acid site mutation analyses of EV-D68.

Chinese soy-based microbiome and associated microbial risks: a metagenomic investigation.

Fermented foods are a longstanding part of the Chinese diet and have been recognized for promoting gut microbial diversity. However, their microbial composition remains poorly defined, raising concerns about potential exposure to pathogens and antibiotic resistance genes (ARGs). Using shotgun metagenomics, we examined microbiota of 93 representative samples spanning three major categories of traditional Chinese fermented soybean products.

Genotypic and phenotypic characteristics of group B streptococcal isolates from neonates in a tertiary hospital in Qingyang, Gansu Province, China.

Group B Streptococcus (GBS) causes severe neonatal infections, leading to high mortality rates. This study aimed to investigate the genetic characteristics of GBS isolates in a hospital setting. Nineteen isolates were identified through a review of preservation and cultivation processes since January 2022, followed by antibiotic susceptibility testing.

Gremlin1 repression-mediated mitochondrial network hyperfunction contributes to TCE-induced zebrafish cardiac defects.

Background: Trichloroethylene (TCE) is a ubiquitous pollutant with potential capacity to induce congenital heart disease (CHD). However, the mechanisms underlying TCE-induced CHD are largely unraveled.

Methods: We exposed zebrafish embryos to TCE to investigate its cardiac development toxicity and related response factor through bulk RNA sequencing.

A TAT Peptide-Functionalized Liposome Delivery Phage System (TAT-Lip@PHM) for an Enhanced Eradication of Intracellular MRSA.

Intracellular bacteria frequently result in chronic and recurrent infections. MRSA is one of the most prevalent facultative intracellular bacteria in clinical infections. The drug resistance of MRSA and the difficulty of most antibiotics in entering cells result in a suboptimal clinical efficacy of antibiotics in the treatment of intracellular MRSA.

Regulatory role of ISEcp1 and spacer sequences in bla-mediated cephalosporin resistance in Shigella.

Background: Extended-spectrum β-lactamases, especially CTX-M enzymes, play a critical role in cephalosporin resistance in several bacterial species. The ISEcp1 insertion sequence, a mobile genetic element, regulates the high expression and horizontal transfer of the bla gene. Diverse types of spacer regions exist, the length of which varies across the different types of bla genes and their upstream ISEcp1 elements.

Detection assay of polymyxin resistance coding gene based on CRISPR/Cas13a system.

Introduction: Polymyxins are reserved as an ultimate defense against multidrug-resistant bacteria. The emergence of the polymyxin resistance gene poses a potential risk for the treatment of severe infections caused by Gram-negative bacteria. Timely detection and monitoring the gene are essential for guiding anti-infective therapy and controlling the spread of polymyxin resistance.

Tunable Ultralong Organic Room-Temperature Phosphorescence of Dinaphthylamine Skeleton via Molecular Modification and Conformational Change in a Flexible Cross-Linked Polymer Network.

Herein, we developed a simple cross-linking strategy to fabricate a flexible PETA/MA polymer network featuring tunable ultralong organic room-temperature phosphorescence (UORTP). Three dinaphthylamine-structured phosphorescence molecules (NQA-1, NQA-2, and NQA-3), modified with an (iso)quinoline ring, were synthesized, and the tiny structural change made a great difference to their UORTP properties by controlling T excited states and intersystem crossing (ISC) efficiency. We thoroughly studied the UORTP performance in toluene solution, in PMMA film, in non-cross-linked copolymerized films, and in cross-linked films with three different cross-linkers.

Portable microfluidic-LAMP assay for rapid on-site detection of eight highly pathogenic viruses.

Background: The recent global spread of highly pathogenic viruses, such as Mpox and Ebola viruses, highlights the urgent need for multiplex detection technologies. Current methods lack the capability for simultaneous identification, which hampers outbreak control in resource-limited settings and global surveillance efforts. Traditional techniques, like real-time quantitative polymerase chain reaction (qPCR), are often impractical in resource-limited areas due to laboratory dependency, and specialized personnel requirements.

Foodborne outbreak of enterotoxigenic Staphylococcus aureus at a school in Hebei, China.

In 2024, a S. aureus foodborne illness outbreak in Hebei Province, China, affected 61 out of 563 individuals (10.84 % prevalence), causing symptoms like diarrhea, vomiting, abdominal pain, and nausea.

Mortality from Alzheimer's disease and other dementias and its heterogeneity across states in India findings from GBD 2021 study.

Dementia is a leading cause of death worldwide. This study examines trends and risk factors for dementia mortality in India and projects the future burden. Mortality and risk factors for Alzheimer's disease and other dementias (ADOD) in India between 1990 and 2021 were extracted from the Global Burden of Disease (GBD) 2021 database.

Molecular Detection of Tick-Borne Bacterial Pathogens in Ticks and Rodents from the China-Vietnam Border.

Ticks and tick-borne diseases constitute a crucial focus for the health of both humans and animals worldwide. Although numerous studies on tick-borne diseases have been conducted in China, reports on tick-borne pathogens in ticks and rodents from the China-Vietnam border are scarce. In this study, we investigated tick-borne bacterial pathogens, including , Anaplasmataceae, and , in nine rodents () and 88 ticks collected from cattle and rodents in Jingxi, a city at the China-Vietnam border.

Targeting LINC00707 by vitamin D3 attenuates nitrogen mustard-caused dermal toxicity through inhibiting ferroptosis.

Nitrogen mustard (NM) causes severe skin injury that is lack of effective and targeted therapies. Vitamin D3 (VD3) emerges as a promising treatment option for NM-caused dermal toxicity; however, the underlying mechanisms are currently unclear. Herein, we identified that NM markedly promoted ferroptosis by measurement of decreased cell viability, glutathione, glutathione peroxidase 4 and solute carrier family 7 member 11 levels, and increased ROS, lipid ROS, iron/Fe and malondialdehyde contents in vitro and in vivo.

Through-Space Electron Effect Regulated Selective Expression of Ultralong Organic Room Temperature Phosphorescence and Photochromism.

We report a through-space electron effect (TSEE) between two phosphorescent units that significantly modulates the selective expression of ultralong organic room-temperature phosphorescence (UORTP) and photochromism. A series of phosphorescent molecules (DNapTr, DNapBNT, DNapFL, and DNapPy), featuring two phosphorescent units connected by a nonconjugated spacer, were synthesized. Our findings reveal that both TSEE and the energy gap Δ (defined as - ) between the lowest triplet energy levels (T) of the phosphorescent units play crucial roles in determining UORTP and the photochromic behavior in PMMA films.

Resolving floral development dynamics using genome and single-cell temporal transcriptome of Dendrobium devonianum.

Dendrobium devonianum, a species of the Orchidaceae family, is notable for its unique floral characteristics, which include two yellow spots and purple tips on its labellum, as well as fringed edges. However, the molecular mechanisms underlying flower pattern formation in D. devonianum remain poorly understood, hindering advancements in its breeding process.

Report of two coinfections of human adenovirus and sapovirus in patients with acute gastroenteritis from China.

Background: Coinfections involving multiple diarrheal viruses have gained increasing recognition as a significant cause of acute gastroenteritis in recent years. Understanding the genetic diversity and evolutionary relationships of these viruses is crucial for effective outbreak identification and tracking.

Objective: To report two cases of HAdV and SaV coinfections and elucidate the genetic diversity and evolutionary patterns of these viruses through whole-genome sequencing (WGS) and phylogenetic analysis.

Prediction of the exposure profile of bisphenol A in Chinese population by physiologically-based pharmacokinetics modeling.

Bisphenol A (BPA) has emerged as a typical contaminant which can induce various adverse effects on human health and environment. BPA exposure and the corresponding human responses vary across different regions and populations. China is one of the largest producers and consumers of BPA, but the exposure profile of BPA in Chinese population is poorly understood.

Derivation of a health-based guidance value for bisphenol A via the weight of evidence approach.

There remains a debate over the health-based guidance value of bisphenol A (BPA) worldwide. Through the weight of evidence approach, this study systematically searched and evaluated the updated BPA toxicological data following the guidelines for evaluating the relevance and reliability of toxicological data developed by the China National Center for Food Safety Risk Assessment. Benchmark dose and no observed adverse effect dose/lowest observed adverse effect level methods were used for dose-relationship analysis.