Marital Failure and Subsequent Heart Failure: A Prospective Study.

Background: Loss of a stable spousal relationship has been associated with a high risk of morbidity and premature death, yet there is limited evidence on the association between marital failure and the risk of subsequent heart failure (HF). We examined the association between marital failure and the risk of subsequent HF in large prospective studies.

Methods: This cohort study utilized data from Kailuan Study I and Kailuan Study II.

Modifiable Factors and 10-Year and Lifetime Risk of Cardiovascular Disease in Adults With New-Onset Diabetes: The Kailuan Cohort Study.

Background: Adults with newly diagnosed diabetes are at elevated risk of cardiovascular disease (CVD). However, the extent to which optimizing modifiable lifestyle and clinical factors can mitigate this risk remains insufficiently assessed across both short- and long-term risk periods.

Methods: This study analyzed 12 222 participants from the Kailuan cohort (2006-2016) who developed new-onset diabetes and were free of baseline CVD.

Protective effects of liraglutide on hypercholesterolemia-associated atherosclerosis involve attenuation of endothelial-monocyte adhesion through down-regulating the LOX-1/NF-κB signaling pathway.

To investigate the impact of liraglutide (LIR) on experimental hypercholesterolemia-associated atherosclerosis and endothelial-monocyte adhesion, as well as elucidate the involvement of lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1)/NF-κB signaling pathway in this process. High-fat diet-fed ApoE mice were treated with LIR (300 mg/kg, subcutaneous injection, twice a week) or normal saline for 6 weeks. Normal diet-fed ApoE and C57BL/6 mice were served as control.

Precise Excited-State Engineering in Benzimidazole Derivatives via Donor-Acceptor Architecture for Organic Temperature-Responsive Phosphorescence.

Herein, we report precise excited-state engineering in benzimidazole (NBd) derivatives via a donor-acceptor (D-A) architecture for organic temperature-responsive phosphorescence (RTP). Four NBd derivatives (1-NBdCN, 1-NBdBrCN, 2-NBdCN, and 2-NBdBrCN) with an electron acceptor at position 1 and position 2 were designed and synthesized. The position of the electron acceptor enables 1-NBdCN and 1-NBdBrCN with temperature-responsive phosphorescence and endows 2-NBdCN and 2-NBdBrCN with red-shifted phosphorescence, showing a remarkable site effect.

Protective effects of quercetin on LPS-induced liver injury in quails.

Quercetin, a natural flavonoid with antioxidant and anti-inflammatory activities, shows promise as a feed additive that reduces antibiotic use and enhances animal production and organ health. This study investigated the protective effects of dietary quercetin against lipopolysaccharide (LPS)-induced liver injury in quail and screened for its optimal dietary concentration. Quails were fed a basal diet supplemented with different concentrations of quercetin (0.

Enhanced Cassava Flour Quality to Improve the Cassava Bread by Attibutes Yeast Fermentation.

Cassava is rich in starch and serves as a food staple in tropical and subtropical regions worldwide. However, cassava contains hydrocyanic acid, which can be harmful to human health in high concentrations. Proper processing methods, such as soaking, fermentation, or cooking, are typically employed to reduce cyanide levels to safe limits (< 10 mg HCN/kg), where it may even exhibit antioxidant properties.

Cardiomyocyte-derived USP13 protects hearts from hypertrophy via deubiquitinating and stabilizing STAT1 in male mice.

Cardiac hypertrophy leads to ventricular dysfunction and heart failure. Deubiquitinating enzymes are responsible for preserving the substrate protein stability and are essential to myocardial hypertrophy. In this study, we aimed to explore the role and regulatory mechanism of a cardiomyocyte-derived deubiquitinating enzyme, USP13, in cardiac hypertrophy.

Enzymatic hydrolysis of cassava leaf proteins unlocks antioxidant and therapeutic peptides: Alcalase-derived bioactives target oxidative stress pathways.

Cassava leaves, often discarded as agricultural byproduct, are a nutrient-dense yet underutilized protein source, containing 38 % proteins by dried weight. This study investigates the potential of cassava leaf proteins as a sustainable alternative to conventional protein sources through enzymatic hydrolysis. Bioactive peptides extracted during hydrolysis significantly improve the protein's nutritional profile and exhibited diverse biological activities.

miR-132 inhibition improves cardiac remodeling and function in a two-hit mouse model of heart failure with a preserved ejection fraction.

To investigate the impact of antimiR-132, a miR-132 antisense inhibitor, on cardiac remodeling and function in a two-hit mouse model of heart failure with preserved ejection fraction (HFpEF), as well as its underlying mechanism. Male C57BL/6 mice were fed N(omega)-nitro-L-arginine methyl ester plus a high-fat diet to establish an HFpEF model, and then intraperitoneally injected with antimiR-132 or normal saline. Cardiac fibroblasts treated with transforming growth factor β1 (TGF-β1) were cultured in the presence of antimiR-132 or vehicle to examine collagen synthesis and potential mechanisms.

Deubiquitinase USP13 alleviates doxorubicin-induced cardiotoxicity through promoting the autophagy-mediated degradation of STING.

Doxorubicin (Dox) is an anthracycline drug widely applied in various malignancies. However, the fatal cardiotoxicity induced by Dox limits its clinical application. Post-transcriptional protein modification ubiquitination/deubiquitination in cardiomyocytes mediates the pathophysiological process in Dox-induced cardiotoxicity (DIC).

Cost-effectiveness of cardiovascular risk assessment and management among adults with hypertension in China: A modelling study.

Objective: Blood pressure reduction alone is insufficient for significant cardiovascular risk improvement in patients with hypertension. Cardiovascular risk assessment and management are recommended to prevent and control cardiovascular diseases (CVD), but their cost-effectiveness in real-world Chinese settings is unclear. This study evaluated the benefits and cost-effectiveness of two strategies based on cardiovascular risk in Chinese hypertensive patients aged ≥35 years: statin treatment and lifestyle management + statin treatment.

Engineering catalytically promiscuous enzymes to serve new functions.

Catalytic promiscuity in enzymes refers to their ability to catalyze multiple chemically distinct reactions in addition to their native activity. The increasing discovery of additional enzymes exhibiting catalytic promiscuity has underscored the significance of this trait in nature. The catalytic promiscuity of enzymes offers new avenues for functional redesign.

Influence of cuff size on the accuracy of supine blood pressure measurement.

Objective: The discrepancy in blood pressure (BP) measurements while in a supine position using an upper-arm automated sphygmomanometer equipped with cuffs of varying sizes remains unresolved.

Methods: In this single-center, randomized crossover trial, a total of 223 inpatients from the Affiliated Hospital of Putian University were enrolled between August and September 2023. Three sets of triplicate BP measurements were obtained while the participants were in a supine position using an automated sphygmomanometer with randomly assigned appropriately sized, undersized, or oversized BP cuffs.

JOSD2 alleviates acute kidney injury through deubiquitinating SIRT7 and negativity regulating SIRT7-NF-κB inflammatory pathway in renal tubular epithelial cells.

Acute kidney injury (AKI), triggered by various stimuli including ischemia-reperfusion, nephrotoxic insult, and sepsis, is characterized by an abrupt deterioration in kidney function. Ubiquitination is a post-translational modification of proteins that plays a critical role in the pathogenesis and progression of AKI. In this study, we aimed to investigate the role and underlying mechanism of the deubiquitinating enzyme Josephin Domain-containing protein 2 (JOSD2) in AKI.

Robust Anion-Pillared Ultramicroporous Material for CH/CH Separation with High CH Uptake and Selectivity.

Removing trace amounts of CH from CH/CH mixtures for CH purification is crucial but extremely challenging. In this work, by introducing SiF with specific recognition for CH, we constructed a robust adsorbent, FJI-W88, with a high CH adsorption capacity and excellent CH/CH separation selectivity. FJI-W88 not only exhibits ultrahigh CH uptake at 0.

Modifiable factors and 10-year and lifetime cardiovascular disease risk in adults with new-onset hypertension: insights from the Kailuan cohort.

Background: Preventing cardiovascular disease (CVD) in adults with hypertension is essential, but it remains uncertain whether optimizing modifiable factors can eliminate the excess CVD risk associated with new-onset hypertension.

Methods: In this prospective cohort study, 29,597 adults with new-onset hypertension and no prior CVD (from 2006-2016 surveys) were each matched by age and sex to a normotensive control. Eight modifiable factors were assessed using the American Heart Association's Life's Essential 8 algorithm.

Epsilon-polylysine microneedle potentiating MXene-mediated photothermal ablation for combating antibiotic-resistant bacterial infections.

Antimicrobial peptides show promise in enhancing photothermal therapy, but their application is often limited by the challenge of constructing a delivery system that balances efficacy and safety. Our research demonstrated that the bactericidal efficacy of VC MXene-mediated photothermal therapy is enhanced in a concentration-dependent relationship with the introduction and coating of the antimicrobial peptide ε-polylysine (EPL). EPL exhibited a dual role in enhancing bacterial binding and disrupting bacterial membranes, thereby increasing heat transfer efficiency and reducing bacterial resistance to photothermal ablation.

Deubiquitinase MYSM1 drives myocardial ischemia/reperfusion injury by stabilizing STAT1 in cardiomyocytes.

Myocardial ischemia/reperfusion (I/R) injury leads to irreversible cardiomyocyte death and aggravates myocardial infarction. Deubiquitinating enzymes (DUBs) are essential for maintaining substrate protein stability and functionality, playing significant roles in cardiac pathophysiology. In this study, we aimed to clarify the regulatory role of a DUB, Myb-like, SWIRM, and MPN domains 1 protein (MYSM1), in myocardial I/R injury and explore the molecular mechanism behind.

ERK-USP9X-coupled regulation of thymidine kinase 1 promotes both its enzyme activity-dependent and its enzyme activity-independent functions for tumor growth.

Thymidine kinase 1 (TK1), a crucial enzyme in DNA synthesis, is highly expressed in various cancers. However, the mechanisms underlying its elevated expression and the implications for tumor metabolism remain unclear. Here we demonstrate that activation of growth factor receptors enhances TK1 expression.

Vericiguat improves cardiac remodelling and function in rats with doxorubicin-induced cardiomyopathy.

Purpose: Vericiguat, a soluble guanylate cyclase (sGC) stimulator, has been demonstrated effective in improving prognosis of patients with heart failure with reduced ejection fraction. However, there are limited data concerning the effect of vericiguat in patients with doxorubicin (DOX)-induced cardiomyopathy (DIC). In this study, we investigated the effects of vericiguat on cardiac structure and function in rats with DIC as well as their potential mechanisms of action.

Deubiquitinase MYSM1 promotes doxorubicin-induced cardiotoxicity by mediating TRIM21-ferroptosis axis in cardiomyocytes.

Anthracycline antitumor drug doxorubicin (DOX) induces severe cardiotoxicity. Deubiquitinating enzymes (DUBs) are crucial for protein stability and function and play a significant role in cardiac pathophysiology. By comparing RNA sequencing datasets and conducting functional screening, we determined that Myb-like, SWIRM, and MPN domains 1 (MYSM1) is a key regulator of DOX-induced cardiotoxicity.

H9 Consensus Hemagglutinin Subunit Vaccine with Adjuvants Induces Robust Mucosal and Systemic Immune Responses in Mice by Intranasal Administration.

The H9N2 subtype avian influenza viruses mainly cause respiratory symptoms, reduce the egg production and fertility of poultry, and result in secondary infections, posing a great threat to the poultry industry and human health. Currently, all H9N2 avian influenza commercial vaccines are inactivated vaccines, which provide protection for immunized animals but cannot inhibit the spread of the virus and make it difficult to distinguish between the infected animals and vaccinated animals. In this study, a trimeric consensus H9 hemagglutinin (HA) subunit vaccine for the H9N2 subtype avian influenza virus based on a baculovirus expression system was first generated, and then the effects of three molecular adjuvants on the H9 HA subunit vaccine, Cholera toxin subunit B (CTB), flagellin, and granulocyte-macrophage colony-stimulating factor (GM-CSF) fused with H9 HA, and one synthetic compound, a polyinosinic-polycytidylic acid (PolyI:C) adjuvant, were evaluated in mice by intranasal administration.

Cardiomyocyte-derived USP28 negatively regulates antioxidant response and promotes cardiac hypertrophy via deubiquitinating TRIM21.

Cardiac hypertrophy is an important pathological basis for heart failure. Most physiological activities of cardiomyocytes are regulated by proteins and their post-translational modification. Deubiquitinating enzymes (DUBs) are involved in protein stability maintenance and closely related to myocardial hypertrophy.

Lipidomic Profiling and Storage-Induced Changes in Cassava Flour Using LC-MS/MS.

Cassava serves as a primary staple food for over one billion people worldwide. The quality of cassava flour is markedly affected by the oxidation and deterioration of lipids during storage. Despite its significance, the lipid composition of cassava flour and its alterations throughout storage periods have not been extensively studied.