Mechanically tunable fiber-based hydrogel activates PIEZO1-integrin axis for enhanced bone repair.

Irregular alveolar bone defects pose persistent clinical challenges due to their complex morphology and the lack of biomaterials that simultaneously provide structural integrity, biocompatibility, and dynamic osteoinductive potential. Herein, we report a fiber-reinforced, dual-network hydrogel system (OHADN fiber@Yoda1 hydrogel) engineered to recapitulate mechanobiological cues for enhanced bone regeneration. This injectable hydrogel integrates oxidized hyaluronic acid (OHA) crosslinked with Yoda1-loaded PLGA-collagen fiber fragments and stabilized via catechol-Fe³⁺ coordination, forming a robust and self-healing structure.

Promoting bone generation through accordion technique in critical-sized bone defects: a review.

Critical-sized bone defects caused by trauma, infection, tumor resection, or congenital malformation pose formidable challenges in orthopedic reconstruction. Distraction osteogenesis (DO) has been extensively applied due to its dual capacity for bone and soft tissue regeneration. However, delayed consolidation often leads to prolonged external fixation, increasing the risk of complications.

[Advances in mechanotransduction signaling pathways in distraction osteogenesis].

Objective: To review the role and research progress of mechanotransduction signaling pathway in distraction osteogenesis, so as to provide theoretical basis and reference for clinical treatment.

Methods: The role and research progress of mechanotransduction signaling pathway in distraction osteogenesis were summarized by extensive review of relevant literature at home and abroad.

Results: The mechanotransduction signaling pathway plays a central role of "sensation-transformation-execution" in distraction osteogenesis, and activates a series of molecular mechanisms to promote the regeneration and remodeling of bone tissue by integrating external mechanical signals.

Analysis of the effects of acetyl tributyl citrate on bone cancer based on network toxicology and molecular docking.

Background: Acetyl tributyl citrate (ATBC) is a widely used environmental plasticizer that has raised concerns regarding its potential health effects, particularly its role in cancer development. Although ATBC is generally considered to have a safer profile compared to traditional phthalate-based plasticizers, research on its association with bone cancer remains limited. The aim of this study is to elucidate the complex effects of Acetyl tributyl citrate (ATBC) on bone cancer and to unravel the potential molecular mechanisms by which environmental pollutants influence the disease process.

Bioinformatics analysis based on microarray data reveals molecular crosstalk and immune relationship between sarcopenia and atherosclerosis.

Background: Several global studies have investigated the association between sarcopenia and atherosclerosis. However, the potential common pathogenesis, molecular mechanisms, and their relationship remain elusive. Through bioinformatic analysis, we aim to identify potential biomarkers and therapeutic targets for sarcopenia and atherosclerosis, providing a theoretical foundation for future research.

Integrating network toxicology and Mendelian randomization to uncover the role of AHR in linking air pollution to male reproductive health.

Background: With the rapid advancement of global industrialization and urbanization, air pollution has emerged as a major public health concern. This study investigates the molecular mechanisms linking air pollutants (APs) to male reproductive health (MRH), providing a scientific foundation for disease prevention and treatment.

Methods: APs-disease-related genes were retrieved from multiple network databases, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses.

Electrostatic Potential Matching in an Anion-Pillared Framework for Benchmark Hexafluoroethane Purification from Ternary Mixture.

One-step purification of CFCF from ternary CFCHF/CFCHF/CFCF mixture is crucial since its vital role in the semiconductor industry. However, efficient separation of chemically inert CF₃CF₃ remains challenging due to the difficulty in creating specific recognition sites in porous materials. In this work, we report the first example of anion-pillared MOFs to the separation of fluorinated electronic specialty gases, utilizing the unique electrostatic potential matching in the bipolar pores of SIFSIX-1-Cu to realize a benchmark CFCHF/CFCHF/CFCF separation.

Robust Anion-Pillared Ultramicroporous Material for CH/CH Separation with High CH Uptake and Selectivity.

Removing trace amounts of CH from CH/CH mixtures for CH purification is crucial but extremely challenging. In this work, by introducing SiF with specific recognition for CH, we constructed a robust adsorbent, FJI-W88, with a high CH adsorption capacity and excellent CH/CH separation selectivity. FJI-W88 not only exhibits ultrahigh CH uptake at 0.

Synergistic Functions of the Janus Fibrous Membrane for Enhanced Bone Repair.

The treatment of bone defects presents significant challenges in clinical practice. Guided bone regeneration (GBR) strategies offer a new approach, but existing commercial GBR membranes still lack optimal barrier and osteogenic functions. This study presents the design of a Janus fibrous membrane using classic amphiphilic block copolymers and gelatin methacryloyl containing unsaturated double bonds through a gradient electrospinning process.

The mechanical test as a supplemental decision support tool for the safe removal of an Ilizarov circular external fixator.

Background: Timing the fixator removal is vital for a successful external fixation treatment. The purpose of this study was to determine the effectiveness of axial load-share ratio in vivo as a supplemental decision support tool for the safe removal of an Ilizarov circular external fixator.

Methods: This prospective observational study consists of 83 patients undergoing tibial or femoral lengthening with Ilizarov circular external fixation in our institution, from January 2011 to October 2019.

Electroactive membranes enhance in-situ alveolar ridge preservation via spatiotemporal electrical modulation of cell motility.

Post-extraction alveolar bone resorption invariably compromises implant placement and aesthetic restoration outcomes. Current non-resorbable membranes exhibit limited efficacy in alveolar ridge preservation (ARP) due to insufficient cell recruitment and osteoinductive capabilities. Herein, we introduce a multifunctional electroactive membrane (PPy-BTO/P(VDF-TrFE), PB/PT) designed to spatiotemporally regulate cell migration and osteogenesis, harmonizing with the socket healing process.

The modified suture-bridge technique for treating avulsion fracture of minors tibial eminence of anterior cruciate ligament: a retrospective study.

Purpose: This study aimed to evaluate the clinical and radiological outcomes of modified suture-bridge technique fixation for anterior cruciate ligament (ACL) tibial avulsion fracture.

Method: Minors who underwent arthroscopic reduction and modified suture bridge fixation of ACL tibial avulsion fracture between January 2018 and January 2022 were retrospectively analyzed. Postoperative MRI and X-ray examinations were performed to evaluate the presence of epiphyseal plate injury and fracture healing.

Bioinformatics and system biology approach to identify potential common pathogenesis for COVID-19 infection and sarcopenia.

Sarcopenia is a condition characterized by age-related loss of muscle mass and strength. Increasing evidence suggests that patients with sarcopenia have higher rates of coronavirus 2019 (COVID-19) infection and poorer post-infection outcomes. However, the exact mechanism and connections between the two is unknown.

Bioinformatics and systems biology approaches to identify potential common pathogeneses for sarcopenia and osteoarthritis.

Sarcopenia, a geriatric syndrome characterized by progressive loss of muscle mass and strength, and osteoarthritis, a common degenerative joint disease, are both prevalent in elderly individuals. However, the relationship and molecular mechanisms underlying these two diseases have not been fully elucidated. In this study, we screened microarray data from the Gene Expression Omnibus to identify associations between sarcopenia and osteoarthritis.

Nomogram construction and validation of axial deviation in patients with tibial defects treated with the Ilizarov bone transport technique.

Introduction: The Ilizarov bone transport technique is widely recognised as an effective method for treating large segment bone defects in clinical practice. However, axial deviation is a common complication in the treatment of tibial large segment bone defects, which can have a serious impact on the clinical efficacy of bone transport. Our study aims to construct and validate a nomogram for predicting axial deviation of tibial bone transport.

Cross-species metabolomic profiling reveals phosphocholine-mediated liver protection from cold and ischemia/reperfusion.

Cold and ischemia/reperfusion (IR)-associated injuries are seemingly inevitable during liver transplantation and hepatectomy. Because Syrian hamsters demonstrate intrinsic tolerance to transplantation-like stimuli, cross-species comparative metabolomic analyses were conducted with hamster, rat, and donor liver samples to seek hepatic cold and IR-adaptive mechanisms. Lower hepatic phosphocholine contents were found in recipients with early graft-dysfunction and with virus-caused cirrhosis or high model for end-stage liver disease scores (≥30).

Sequential glycosylations at the multibasic cleavage site of SARS-CoV-2 spike protein regulate viral activity.

The multibasic furin cleavage site at the S1/S2 boundary of the spike protein is a hallmark of SARS-CoV-2 and plays a crucial role in viral infection. However, the mechanism underlying furin activation and its regulation remain poorly understood. Here, we show that GalNAc-T3 and T7 jointly initiate clustered O-glycosylations in the furin cleavage site of the SARS-CoV-2 spike protein, which inhibit furin processing, suppress the incorporation of the spike protein into virus-like-particles and affect viral infection.

Medial patellar ligament reconstruction in combination with derotational distal femoral osteotomy for treating recurrent patellar dislocation in the presence of increased femoral anteversion: a systematic review.

Background: Medial patellar ligament reconstruction (MPFL-R) in combination with derotational distal femoral osteotomy (DDFO) for treating recurrent patellar dislocation (RPD) in the presence of increased femoral anteversion is one of the most commonly used surgical techniques in the current clinical practice. However, there are limited studies on the clinical outcomes of MPFL-R in combination with DDFO to treat RPD in the presence of increased femoral anteversion.

Purpose: To study the role of MPFL-R in combination with DDFO in the treatment of RPD in the presence of increased femoral anteversion.

Injectable ultrasound-powered bone-adhesive nanocomposite hydrogel for electrically accelerated irregular bone defect healing.

The treatment of critical-size bone defects with irregular shapes remains a major challenge in the field of orthopedics. Bone implants with adaptability to complex morphological bone defects, bone-adhesive properties, and potent osteogenic capacity are necessary. Here, a shape-adaptive, highly bone-adhesive, and ultrasound-powered injectable nanocomposite hydrogel is developed via dynamic covalent crosslinking of amine-modified piezoelectric nanoparticles and biopolymer hydrogel networks for electrically accelerated bone healing.

Synergy of 5-aminolevulinate supplement and CX3CR1 suppression promotes liver regeneration via elevated IGF-1 signaling.

Inadequate remnant volume and regenerative ability of the liver pose life-threatening risks to patients after partial liver transplantation (PLT) or partial hepatectomy (PHx), while few clinical treatments focus on safely accelerating regeneration. Recently, we discovered that supplementing 5-aminolevulinate (5-ALA) improves liver cold adaptation and functional recovery, leading us to uncover a correlation between 5-ALA metabolic activities and post-PLT recovery. In a mouse 2/3 PHx model, 5-ALA supplements enhanced liver regeneration, promoting infiltration and polarization of anti-inflammatory macrophages via P53 signaling.

Evaluation of the clinical performance of p16/Ki-67 dual-staining cytology for cervical lesion detection in premenopausal and postmenopausal Chinese women.

Background: Studies on the clinical performance of p16/Ki-67 dual-staining in detecting cervical lesions by menopausal status were limited.

Methods: 4364 eligible women were enrolled with valid p16/Ki-67, HR-HPV, and LBC test results, including 542 cancer and 217 CIN2/3 cases. The positivity rates of p16 and Ki-67 single staining and p16/ Ki-67 dual-staining were analyzed by different pathological grades and age groups.

Towards a Nonperturbative Formulation of the Jet Charge.

The jet charge is an old observable that has proven uniquely useful for discrimination of jets initiated by different flavors of light quarks, for example. In this Letter, we propose an approach to understanding the jet charge by establishing simple, robust assumptions that hold to good approximation nonperturbatively, such as isospin conservation and large particle multiplicity in the jets, forgoing any attempt at a perturbative analysis. From these assumptions, the jet charge distribution with fixed particle multiplicity takes the form of a Gaussian by the central limit theorem and whose mean and variance are related to fractional-power moments of single particle energy distributions.

Accelerated Bone Reconstruction by the Yoda1 Bilayer Membrane via Promotion of Osteointegration and Angiogenesis.

Guided bone regeneration membranes are widely used to prevent fibroblast penetration and facilitate bone defect repair by osteoblasts. However, the current clinically available collagen membranes lack bone induction and angiogenic capacities, exhibiting limited bone regeneration. The mechanically sensitive channel, Piezo1, which is activated by Yoda1, has been reported to play crucial roles in osteogenesis and angiogenesis.