Identification of hypoxia-related diagnostic biomarkers and immune signatures in diminished ovarian reserve.

Background: Diminished ovarian reserve (DOR) becomes more common with age, and hypoxia is a key cause of apoptosis in ovarian granulosa cells. This study investigated the genetic links between hypoxia and DOR.

Methods: The GSE87201 dataset for DOR was sourced from Gene Expression Omnibus database, normalized for common differentially expressed genes (Co-DEGs), and identified Hypoxia-related differentially expressed genes (HRDEGs) GeneCards; Receiver Operating Characteristic (ROC) curves evaluated HRDEGs' diagnostic value, and protein-protein interaction networks were visualized with STRING and Cytoscape.

SIRT6 Lysine-Demyristoylates ATF2 to Ameliorate Vascular Injury via PRKCD/VE-Cadherin Pathway Regulating Vascular Endothelial Barrier.

Cardiovascular diseases (CVDs) progression is significantly modulated by epigenetic mechanisms, particularly through Sirtuin 6 (SIRT6), a key NAD⁺-dependent deacetylase in the sirtuin family. Though essential for cardiovascular homeostasis, the effects of SIRT6-mediated lysine myristoylation on CVDs progression remain largely unexplored due to detection limitations. This study developes an innovative lysine-myristoylated peptide enrichment technique, identifying mutant SIRT6 (H133Y) with high myristoyl affinity but deficient demyristoylase activity.

Rat Model of Granulomatous Mastitis.

Background: Granulomatous mastitis (GM) is an immune-related, clinically refractory disease, the translational research of which has been hindered by the lack of animal models. Establishing a stable rat model of GM is critical for elucidating its mechanisms and developing therapeutic approaches.

Methods: The first batch of rats was randomly divided into a normal group, a low-dose group (0.

Organelle regulation of SUMOylation: regulatory mechanisms and therapeutic application.

Background: SUMOylation is a pivotal post-translational modification (PTM) that has been extensively investigated in nuclear processes. However, its role in regulating suborganelle homeostasis warrants further exploration. Recent studies have highlighted the significance of SUMOylation in modulating mitochondrial dynamics and endoplasmic reticulum stress (ERS), among other cellular processes, thereby offering novel insights into disease pathogenesis.

Piezoelectric PVDF membranes for emulsion separation with constant flux and high efficiency.

Emulsion separation, a focal and challenging aspect of oil-water separation processes, has long been a source of frustration for researchers due to the phenomenon of flux decline caused by concentration polarization and adhesion of oil droplets during the separation process. Attempts have been made to address membrane fouling issues through catalytic degradation and bubble flotation methods; however, the flux decline phenomenon persisted. In this work, during the fabrication process, intermolecular forces were utilized to polarize polyvinylidene difluoride molecular chains to increase the β-phase and endow them with piezoelectric properties.

Identifying potential drugs for treating Cardiovascular-kidney metabolic syndrome via reverse network pharmacology.

Background: Cardiovascular, Kidney and metabolic syndrome (CKM) is a complex disease, for which current therapeutic approaches have limited efficacy. This study aims to screen for potential targets and novel drugs for treating CKM using network pharmacology.

Methods: Using reverse network pharmacology, core targets and potential drugs for CKM were identified.

ATRA upregulates OTUD6B to recruit CD8 T cells to suppress colorectal liver metastasis by stabilizing DDX5/STAT3/CXCL11 axis.

OTU deubiquitinase 6B (OTUD6B) study in tumors is gradually increasing; however, studies on the role of OTUD6B in colorectal cancer (CRC) are rare. OTUD6B was overexpressed in some human CRC and liver metastasis samples. Although OTUD6B facilitated migration and invasion in CRC cells, it exhibited opposite effects on liver metastasis in immunodeficient and immunocompetent mice.

Unraveling evolutionary pathways: allopolyploidization and introgression in polyploid Prunus (Rosaceae).

Allopolyploidization, resulting from hybridization and subsequent whole-genome duplication (WGD), is a fundamental mechanism driving evolutionary diversification across various lineages within the Tree of Life. The polyploid Prunus (Rosaceae), significant for its economic and agricultural value, provides an ideal model for investigating the evolutionary dynamics associated with allopolyploidy. In this study, we utilized deep genome skimming (DGS) data to demonstrate a comprehensive analytical framework for elucidating the underlying allopolyploidy that includes a newly adapted tool (DGS-Tree2GD) tailored explicitly for accurately detecting WGD events.

Syndecan-1-mediated loading of VEGF-A into small extracellular vesicles facilitates peritubular capillary repair during AKI-to-CKD transition.

Acute kidney injury (AKI) is a severe clinical event with a high risk of transitioning to chronic kidney disease (CKD). Persistent hypoxia resulting from peritubular capillaries (PTCs) loss is a key factor driving this chronic transition. However, the mechanisms governing PTCs injury and repair, particularly under different AKI severity, remain unclear.

Metabolomic and evolutionary integration unveils medicinal potential in six Corydalis species.

The Corydalis genus, one of the largest within the Papaveraceae family, holds a rich diversity of medicinal resources, particularly among its tuberous species. Benzylisoquinoline alkaloids (BIAs) are primarily responsible for the medicinal properties observed in Corydalis plants. In this study, we conducted an integrated evolutionary analysis by combining whole-genome resequencing with comprehensive metabolite profiling across various Corydalis species.

Pore Structure Optimization of a Silica Porous Ceramic Ultrafiltration Membrane for Highly Efficient O/W Emulsion Separation.

The ceramic membrane with chemical, thermal, and mechanical stability is expected to realize oil-in-water (O/W) emulsion separation with high efficiency. A simple and cost-effective approach was adopted to fabricate a three-dimensional (3D) silica porous ceramic ultrafiltration membrane, leveraging diatomite as an abundant and economical mineral resource. A porous structure with high porosity and good mechanical properties was prepared by a sacrificial template method.

Overlapping and specific bacterial communities in the gut and reproductive system of (Hendel) (Diptera: Tephritidae) adults.

Background: Different insect tissues represent heterogeneous niches with distinct physiological and biochemical characteristics, and therefore host different bacterial communities.

Methods: In this study, those overlapping and specific bacterial communities in the female gut (fG), male gut (mG), female reproductive system (fR), and male reproductive system (mR) of (Hendel) adults were determined by high-throughput sequencing targeting 16S rRNA gene.

Results: The richness of bacterial taxa based on OTU was higher in fR compared to the other three tissues.

A liquid biopsy approach detects HCC and identifies GJA4 as a potential biomarker for HBV-HCC via plasma cfDNA methylome profiling.

Background: Early detection of hepatocellular carcinoma (HCC) can greatly improve the survival rate of patients. Plasma cfDNA methylation has been shown to have the potential to be a non-invasive method for diagnosing HCC. However, the identified HCC plasma cfDNA methylation sites were less sensitive to early HCC diagnosis.

The potential mechanisms of reciprocal regulation of gut microbiota-liver immune signaling in metabolic dysfunction-associated steatohepatitis revealed in multi-omics analysis.

As a commonly known aggressive liver-related manifestation within the spectrum of metabolic syndrome with a significant risk of progressing to cirrhosis and hepatocellular carcinoma, metabolic dysfunction-associated steatohepatitis (MASH) is closely intertwined with obesity, insulin resistance, and dyslipidemia. Although the gut microbiota is implicated in MASH progression, the underlying mechanisms require further investigation. In this study, we sought to combine the analysis of the liver transcriptome, circulating metabolome, and gut microbiota to investigate the potential molecular mechanisms underlying the reciprocal regulation between gut microbiota and liver immune signaling.

Decoding the Metabolic-immune Axis: Neutrophil glycolysis-driven tumor niche remodeling and its therapeutic exploitation.

Neutrophils are an important component of the tumor microenvironment, with the majority of previous research concentrating on their anti-tumor role. Recent research indicates that elevated neutrophil-lymphocyte ratios are indicative of worse cancer prognoses. In addition, it revealed the functional transition of neutrophils are related with metabolic regulation within the tumor microenvironment.

Lipidomics Facilitates the Discovery of Diagnostic Biomarkers in Patients with Chronic Total Occlusion during the Perioperative Period.

Chronic total occlusion (CTO) is a subtype of cardiovascular disease associated with high mortality and an increased risk of ventricular arrhythmia. This study aimed to investigate lipidomic changes in CTO patients undergoing percutaneous coronary intervention (PCI) using a tandem-lipidomic strategy. We first applied a global lipidomic approach to identify the serum lipidomes of CTO-PCI patients during the perioperative period, successfully separating and identifying over 1500 lipids.

Tryptophan metabolites exert potential therapeutic activity in graves' orbitopathy by ameliorating orbital fibroblasts inflammation and proliferation.

Purpose: Graves' orbitopathy (GO) is a sight-threatening organ-specific autoimmune disease with complicated pathogenesis. Gut microbiota-derived tryptophan (Trp) metabolites play important roles in immune-related diseases, but their role in GO remains unknown.

Methods: Trp metabolism-associated gut flora was analyzed by 16 S sequencing in GO patients and controls.

Inhibition effects of Eucalyptus globules Labill. essential oil against tyrosinase.

Essential oils derived from Eucalyptus globules Labill. (EGEOs) represent a significant class of bioactive metabolites with broad applications in medicinal and pharmaceutical industries. Despite their various biological activities, the potential of EGEOs to inhibit tyrosinase, a key enzyme in melanin biosynthesis, remains unexplored.

Homocysteine stimulates orbital fibroblast proliferation and migration in Graves' orbitopathy via activating Akt signaling pathway.

Background: Graves' orbitopathy (GO) is a sight-threatening disease associated with thyroid dysfunction, with an unmet medical need for early diagnosis and treatment. Orbital fibroblasts (OFs) proliferation and migration play central roles in the pathogenesis of GO. Homocysteine (Hcy) has been demonstrated to be related to thyroid function, but its role in GO remains unclear.

Age-specific relationships between free triiodothyronine and biological aging.

Objective: Our study aims to examine the associations of thyroid function parameters with phenotypic age acceleration (PhenoAgeAccel) in different age groups.

Method: The analysis included 7,564 participants from the NHANES 1999-2018. PhenoAgeAccel was defined as calculated phenotypic age (PhenoAge) exceeding chronological age.

Clinical characteristics and managements of severe corneal ulcers in patients with end-stage glaucoma.

Purpose: To review the clinical characteristics and managements of severe corneal ulcers in patients with end-stage glaucoma.

Methods: Retrospectively reviewed the medical records of patients diagnosed with severe corneal ulcers who had a history of end-stage glaucoma, including demographic profiles, medical histories, clinical features, and treatments.

Results: Thirty patients (30 eyes) were included.

Orientational Assembly of Carbon Dots-Enabled Electrochemiluminescence Sensors for Ultrasensitive Detection of Halogenated Phenols.

Developing simple, sensitive, and miniaturized sensors is crucial for the prevention and control of halogenated phenolic pollutants exposed to the environment. In this work, oriented carbon dot assemblies are developed for the electrochemiluminescence (ECL) sensing of halogenated phenolic pollutants for the first time. Leveraging liquid-liquid phase separation during ternary solution evaporation, carbon dots (C-dots) self-organize into mesoporous structures (o-Cdots) and granular structures (r-Cdots) on the hydrophobic glass carbon surface, enabling immobilized C-dot ECL sensors.

Incomplete lineage sorting and introgression among genera and species of Liliaceae tribe Tulipeae: insights from phylogenomics.

Background: Phylogenetic research in Tulipa (Liliaceae), a genus of significant economic and horticultural value, has relied on limited nuclear (mostly nuclear ribosomal internal transcribed spacer, nrITS) and plastid DNA sequences, resulting in low-resolution phylogenetic trees and uncertain intrageneric classifications. The genus, noted for its large genome, presents discordant relationships among Amana, Erythronium, and Tulipa, likely due to incomplete lineage sorting (ILS) and/or reticulate evolution. Thus, phylogenomic approaches are needed to clarify these relationships and the conflicting signals within the tribe Tulipeae.

S100A9/RAGE pathway regulation of mitophagy and the effect of JianPi LiShi YangGan formula in acute-on-chronic liver failure.

Ethnopharmacological Relevance: Mitophagy regulates cellular homeostasis and liver inflammation; however, it is inhibited in acute-on-chronic liver failure (ACLF), which drives disease progression. The JianPi LiShi YangGan formula (YGF) has the potential to improve inflammatory responses and reduce mortality in patients with ACLF. However, the precise mechanisms underlying these effects remain unknown.

Identification of the Role of Necroptosis-Related Genes in the Oxidative Damage of Lens Epithelial Cells and Validation in Ultraviolet B-induced Cataract in Rats.

Introduction: The specific role of necroptosis in the pathogenesis of cataracts remains unclear. This study aimed to identify and validate the genes related to necroptosis in the development of cataracts through bioinformatics analysis.

Method: We utilized RNA sequencing data (GSE161701) from the Gene Expression Omnibus (GEO) database and employed R software to perform differential expression analysis of necroptosis- related genes (NRGs) in lens epithelial cells (LECs) under oxidative stress.