Article Synopsis
- Macrophage-derived reactive oxygen species (ROS) trigger the formation of aggresomes, leading to an increase in dormant bacterial persisters that are resistant to antibiotics.
- These dormant bacteria show low ATP levels and metabolic activity, yet they can reactivate when conditions improve.
- The study highlights the potential of targeting bacterial aggresomes as a new strategy to address antibiotic persistence in infections.
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Article Abstract
In this study, we reveal that macrophage-derived reactive oxygen species (ROS) can trigger the rapid formation of aggresomes, which substantially contribute to the increased frequency of persisters induced by phagocytosis. containing aggresomes exhibited a dormant phenotype characterized by reduced adenosine triphosphate (ATP) levels and decreased metabolic activity. Furthermore, these dormant bacteria showed upregulated expression of pathogenicity island 1 (SPI-1)-encoded type III secretion system (T3SS)-related genes, followed by later expression of SPI-2 T3SS-related genes when macrophages ROS production declined. Our results demonstrate that containing aggresomes can enter a dormant state to escape antibiotic attack, while crucially maintaining the ability to resuscitate when the stress environment is improved. Research on bacterial aggresomes could potentially provide therapeutic strategies to combat bacterial antibiotic persistence.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12371251 | PMC |
| http://dx.doi.org/10.1002/imt2.70059 | DOI Listing |
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- These dormant bacteria show low ATP levels and metabolic activity, yet they can reactivate when conditions improve.
- The study highlights the potential of targeting bacterial aggresomes as a new strategy to address antibiotic persistence in infections.
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