Diosmetin Alleviates Cerebral Ischemia/Reperfusion Injury by Modulating Tryptophan Metabolism through the Microbiota-Gut-Brain Axis.

Cerebral ischemia-reperfusion (I/R) injury involves brain tissue damage caused by a temporary interruption of blood flow followed by reperfusion, leading to inflammation, oxidative stress, and neuronal death. Diosmetin, a flavonoid with known anti-inflammatory and antioxidant properties, has been shown to mitigate I/R injury, but its impact on systemic inflammation is not fully understood. In this study, we investigated its protective effects on I/R injury and the mechanisms mediated through the microbiota-gut-brain axis.

Systematic decoding of functional enhancer connectomes and risk variants in human glioma.

Genetic and epigenetic variations contribute to the progression of glioma, but the mechanisms underlying these effects, particularly for enhancer-associated genetic variations in non-coding regions, still remain unclear. Here we performed high-throughput CRISPR interference screening to identify pro-tumour enhancers in glioma cells. By integrating genome-wide H3K27ac HiChIP data, we identified the target genes of these pro-tumour enhancers and revealed the essential role of enhancer connectomes in promoting glioma progression.

Flow Field Reconstruction and Prediction of Powder Fuel Transport Based on Scattering Images and Deep Learning.

This paper presents the flow field reconstruction and prediction of powder fuel transport systems based on representative feature extraction from scattering images using deep learning techniques. A laboratory-built powder fuel supply system was used to conduct scattering spectroscopy experiments on boron-based fuel under various flow rate conditions. Based on the acquired scattering images, a prediction and reconstruction method was developed using a deep network framework composed of a Stacked Autoencoder (SAE), a Backpropagation Neural Network (BP), and a Long Short-Term Memory (LSTM) model.

Time-division multiplexing binocular stereo holography based on persistence of vision for 3D display.

Holography, as a true 3D light-field reconstruction technology, is widely regarded as a solution to the convergence-accommodation conflict (VAC) inherent in conventional stereoscopic displays. While most existing studies prioritize single-viewpoint quality and computational efficiency, this work introduces a time-division multiplexing binocular holographic display system leveraging human persistence of vision. The proposed TDM-BSH system includes an IFrTA-BG and a time-division multiplexing reconstructed method.

Klf5-adjacent super-enhancer functions as a 3D genome structure-dependent transcriptional driver to safeguard ESC identity.

Cell-specific super-enhancers (SEs) and master transcription factors (TFs) dynamically remodel embryonic stem cell (ESC) fate, yet their regulatory interplay remains unclear. By integrating multi-omics data (H3K27ac, Hi-C, scRNA-seq) across ESC states, we identified SEs interacting with master TFs, exemplified by the Klf5-adjacent SE (K5aSE). K5aSE deletion impaired proliferation, differentiation, and Klf5 expression, partially rescued by KLF5 reintroduction.

Detection assay of polymyxin resistance coding gene based on CRISPR/Cas13a system.

Introduction: Polymyxins are reserved as an ultimate defense against multidrug-resistant bacteria. The emergence of the polymyxin resistance gene poses a potential risk for the treatment of severe infections caused by Gram-negative bacteria. Timely detection and monitoring the gene are essential for guiding anti-infective therapy and controlling the spread of polymyxin resistance.

Structural and functional insights into metal coordination and substrate recognition of Akkermansia muciniphila sialidase Amuc_1547.

Sialidases in Akkermansia muciniphila are pivotal for mucin degradation, enabling energy acquisition, modulating gut microbiota balance, and influencing host health. However, their structural and functional mechanisms remain poorly characterized. This study resolved the magnesium-bound crystal structure of Amuc_1547, revealing a six-bladed β-propeller fold linked to a carbohydrate-binding module (CBM)-like β-sandwich domain.

Huperzine A attenuates epileptic seizures via enhancing dCA1-projecting septal cholinergic transmission.

Cholinergic transmission, independent of classical glutamatergic and GABAergic signaling, critically plays a crucial role in epilepsy. Huperzine A (Hup A), an acetylcholinesterase (AChE) inhibitor, exerts potent anticonvulsant activity, but its mechanism of action within cholinergic circuits remains unclear. Here, we show that Hup A mitigates epileptic seizures by enhancing hippocampal dorsal CA1 (dCA1)-projecting cholinergic transmission.

Synergistic microalgae-duckweed systems for enhanced aquaculture wastewater treatment, biomass recovery, and CO sequestration: A novel approach for sustainable resource recovery.

Current aquaculture practices generate nutrient-rich effluents that cause significant environmental pollution. This study presents a novel synergistic microalgae-duckweed system integrating Chlorella sp. and Spirodela polyrhiza for sustainable wastewater treatment, biomass valorization, and carbon sequestration.

Stabilizing Lattice Oxygen to Enable Durable MnO Electrocatalyst for Simultaneous Acidic Hydrogen Production and Biomass Valorization.

Understanding and steering the stability of earth-abundant electrocatalysts in acidic medium is essential for proton exchange membrane (PEM) water electrolyzer. Manganese oxide (MnO) is one of the promising candidates for acidic oxygen evolution reaction (OER), but it still suffers from the overoxidation and the underlying mechanism remains elusive. Here, we observed that lattice oxygen was involved in the OER process on γ-MnO via Mars-van-Krevelen mechanism.

The role of Chinese folk ritual music in biodiversity conservation: an ethnobiological perspective from the Lingnan region.

Background: Traditional ecological knowledge embedded in cultural practices is vital for biodiversity conservation but is increasingly under threat from modernization and globalization. This study investigates the role of Chinese folk ritual music in promoting biodiversity conservation, with a focus on Cantonese Opera and agricultural rituals in the Lingnan region of southern China.

Methods: We employed literature review, qualitative fieldwork, and ethnographic analysis, including interviews with local musicians and community members, to investigate how Cantonese Opera and agricultural rituals contribute to ecological sustainability by integrating cultural narratives with environmental stewardship.

Utilization of Microalgae and Duckweed as Sustainable Protein Sources for Food and Feed: Nutritional Potential and Functional Applications.

Aquatic biomass, particularly microalgae and duckweed, presents a promising and sustainable alternative source of plant-based protein and bioactive compounds for food and feed applications. This review highlights the nutritional potential of these aquatic species, focusing on their high protein content, rapid growth rates, and adaptability to nonarable environments. Microalgae, such as and spp.

Arsenic-induced modulation of virulence and drug resistance in Pseudomonas aeruginosa.

Arsenic contamination of water sources, whether from natural or industrial origins, represents a significant risk to human health. However, its impact on waterborne pathogens remains understudied. This research explores the effects of arsenic exposure on the opportunistic pathogen Pseudomonas aeruginosa, a bacterium found in diverse environments.

Transcriptomics and Proteomics Analysis of the Liver of Knockout Mice.

RAD52 plays crucial roles in several aspects of mammalian cells, including DNA double-strand breaks repair, viral infection, cancer development, and antibody class switching. To comprehensively elucidate the role of RAD52 in maintaining genome stability and uncover additional functions of RAD52 in mammals, we performed the transcriptomics and proteomics analysis of the liver of knockout mice. Transcriptomics analysis reveals overexpression of mitochondrial genes in the liver of knockout (RAD52KO) mice.

Mechanisms and Impact of Rhizosphere Microbial Metabolites on Crop Health, Traits, Functional Components: A Comprehensive Review.

Current agricultural practices face numerous challenges, including declining soil fertility and heavy reliance on chemical inputs. Rhizosphere microbial metabolites have emerged as promising agents for enhancing crop health and yield in a sustainable manner. These metabolites, including phytohormones, antibiotics, and volatile organic compounds, play critical roles in promoting plant growth, boosting resistance to pathogens, and improving resilience to environmental stresses.

Significant Enhancement Catalytic Activity of Nitrile Hydratase by Balancing the Subunits Expression.

Escherichia coli (E. coli) is the most commonly used bacterial recombinant protein production system due to its easy genetic modification properties. In our previous study, a recombinant plasmid expressing the Fe-type nitrile hydratase derived from Rhodococcus erythropolis CCM2595 (ReNHase) was successfully constructed and the recombinant ReNHase exerted an excellent catalytic effect on dinitrile compounds.

EMS: A Large-Scale Eye Movement Dataset, Benchmark, and New Model for Schizophrenia Recognition.

Schizophrenia (SZ) is a common and disabling mental illness, and most patients encounter cognitive deficits. The eye-tracking technology has been increasingly used to characterize cognitive deficits for its reasonable time and economic costs. However, there is no large-scale and publicly available eye movement dataset and benchmark for SZ recognition.

Advancements in multi-omics for nutraceutical enhancement and traits improvement in buckwheat.

Buckwheat ( spp.) is a typical pseudocereal, valued for its extensive nutraceutical potential as well as its centuries-old cultivation. Tartary buckwheat and common buckwheat have been used globally and become well-known nutritious foods due to their high quantities of: proteins, flavonoids, and minerals.

Prospects of Pseudomonas in Microbial Fuel, Bioremediation, and Sustainability.

Microbial applications in agriculture and industry have gained significant attention due to their potential to address environmental challenges and promote sustainable development. Among these, the genus Pseudomonas stands out as a promising candidate for various biotechnological uses, thanks to its metabolic flexibility, resilience, and adaptability to diverse environments. This review provides a comprehensive overview of the current state and future prospects of microbial fuel production, bioremediation, and sustainable development, focusing on the pivotal role of Pseudomonas species.

Septal stimulation attenuates hippocampal seizure with subregion specificity.

Objective: Deep brain stimulation (DBS) is a promising approach for the treatment of epilepsy. However, the optimal target for DBS and underlying mechanisms are still not clear. Here, we compared the therapeutic effects of DBS on distinct septal subregions, aimed to find the precise targets of septal DBS and related mechanisms for the clinical treatment.

Molecular mechanism of the one-component regulator RccR on bacterial metabolism and virulence.

The regulation of carbon metabolism and virulence is critical for the rapid adaptation of pathogenic bacteria to host conditions. In Pseudomonas aeruginosa, RccR is a transcriptional regulator of genes involved in primary carbon metabolism and is associated with bacterial resistance and virulence, although the exact mechanism is unclear. Our study demonstrates that PaRccR is a direct repressor of the transcriptional regulator genes mvaU and algU.

Molecular mechanism of siderophore regulation by the Pseudomonas aeruginosa BfmRS two-component system in response to osmotic stress.

Pseudomonas aeruginosa, a common nosocomial pathogen, relies on siderophores to acquire iron, crucial for its survival in various environments and during host infections. However, understanding the molecular mechanisms of siderophore regulation remains incomplete. In this study, we found that the BfmRS two-component system, previously associated with biofilm formation and quorum sensing, is essential for siderophore regulation under high osmolality stress.

Pseudomonas aeruginosa two-component system CprRS regulates HigBA expression and bacterial cytotoxicity in response to LL-37 stress.

Pseudomonas aeruginosa is a highly pathogenic bacterium known for its ability to sense and coordinate the production of virulence factors in response to host immune responses. However, the regulatory mechanisms underlying this process have remained largely elusive. In this study, we investigate the two-component system CprRS in P.