Using robotic single-port vNOTES for gynaecological oncology: omentectomy in a patient with an ovarian granulosa cell tumor-a case study.

Background: Robotic single-port transvaginal natural orifice transluminal endoscopic surgery (RSP-vNOTES) is an emerging minimally invasive approach that combines the advantages of robotic surgery with scarless transvaginal access. Its application in gynecologic oncology remains limited, particularly for omentectomy during ovarian cancer staging.

Methods: We present the case of a 45-year-old woman with an ovarian granulosa cell tumor (GCT) who underwent supplemental staging surgery following unilateral oophorectomy.

Discovery and Characterization of a Single-Component Halogenase for Phenazine Halogenation.

Halogenated phenazines hold promise as antimicrobial and antibiofilm agents, yet are mainly accessed via chemical synthesis. Herein, we report PezW, a novel single-component flavin-dependent halogenase (FDH) that halogenates phenazine scaffolds, notably enabling enzymatic synthesis of bioactive 2-bromo-1-hydroxyphenazine () and 2,4-bromo-1-hydroxyphenazine (). Structural modeling and mutagenesis revealed key residues critical for substrate binding and catalysis.

SIRT1 as a potential target for age-related eye diseases: mechanisms and therapeutic strategies.

Age-related eye diseases (AREDs) are the leading cause of visual impairment in the elderly, affecting the structure of the anterior and posterior segments of the eye, significantly reducing the quality of life of patients, and even leading to irreversible blindness. Typical AREDs include age-related cataract (ARC), dry eye disease (DED), age-related macular degeneration (AMD), glaucoma, and diabetic retinopathy (DR), the global prevalence of which continues to rise, becoming a serious public health concern. SIRT1 is an NAD + dependent deacetylase, which plays an important physiological regulatory role in ocular tissues, mainly affecting gene expression and various cellular processes by regulating the acetylation status of substrate proteins.

Development and validation of a machine learning model for predicting immune checkpoint inhibitor efficacy in advanced gastric cancer using dynamic changes in peripheral blood clinlabomics data: a retrospective multicenter cohort study.

Background: Immune checkpoint inhibitors (ICIs) play a pivotal role in the treatment of advanced gastric cancer (GC). However, the biomarkers used to predict ICI efficacy are limited due to their reliance on single or static tumor characteristics. This study aims to develop a machine learning (ML) model that incorporates dynamic changes in clinlabomics data to optimize the predictive accuracy of ICI efficacy.

Dynamic data balancing strategy-based Xception-dual-channel LSTM model for laparoscopic cholecystectomy phase recognition.

Purpose: To enhance the temporal feature learning capability of the laparoscopic cholecystectomy phase recognition model and address the class imbalance issue in the training data, this paper proposes an Xception-dual-channel LSTM fusion model based on a dynamic data balancing strategy.

Methods: The model dynamically adjusts the undersampling rate for each surgical phase, extracting short video clips from the original data as training samples to balance the data distribution and mitigate biased learning. The Xception model, utilizing depthwise separable convolutions, extracts fundamental visual features frame by frame, which are then passed to a dual-channel LSTM network.

Release of polycyclic aromatic hydrocarbons from tire road wear particles in simulated digestive fluids: release rules and influencing factors.

The behavior of polycyclic aromatic hydrocarbons (PAHs) released from tire road wear particles (TRWPs) in human digestive fluids may pose a significant risk to human health. However, the current understanding of the release pattern and influencing factors of PAHs from TRWPs is still insufficient. In this study, the release characteristics of PAHs from UV-aging TRWPs (ATRWPs) were systematically investigated by in vitro digestive simulation experiments, release kinetic model fitting and control variable experiments.

Clinical applied anatomical study between the uncinate process of the cervical spine and the vertebral artery.

Objective: Reveal the changing rule of the positional relationship between the uncinate process of cervical spine and vertebral artery by measuring the relevant parameters between the uncinate process of cervical spine and vertebral artery in different age groups.

Methods: A retrospective study was conducted on 1240 cases of cervical spine imaging data from 2018 to 2021 in the Radiology Department of the Affiliated Hospital of Inner Mongolia Medical University. The distance between the uncinate process superior ridge and vertebral artery and the maximum of pedicle transverse angle, the minimum of pedicle transverse angle, the range of pedicle transverse angle and the pedicle width were measured according to age groups.

Excess copper exposure and male reproductive toxicity: molecular mechanisms and potential interventions.

Copper (Cu) is an essential micronutrient in various enzymatic and physiological functions. However, excessive copper intake, mainly resulting from industrial emissions and improper agricultural practices, has raised growing concerns due to its toxicological effects, particularly on the male reproductive system. This review summarizes current research progress on copper-induced reproductive toxicity in males, emphasizing its impact on sperm quality, androgen production, and testicular structure and function.

The molecular mechanisms and therapeutic implications of PANoptosis in ischemic diseases.

The singular forms of programmed cell death (PCD), including pyroptosis, apoptosis, and necroptosis, are inadequate for comprehensively elucidating the complex pathological mechanisms underlying ischemic diseases. PANoptosis is a unique lytic, innate immune, and inflammatory cell death pathway, initiated by innate immune sensors and driven by caspases and RIPKs through PANoptosome complexes. In diseases like cerebral ischemia, retinal ischemia, myocardial ischemia, renal ischemia, and spinal cord ischemia, targeting key regulatory factors of PANoptosis can help mitigate tissue damage.

Multi-Omics Profiling Unveils Antifungal Secondary Metabolites Mediating LQ's Biocontrol Efficacy Against .

is a significant phytopathogen in both pre- and postharvest stages of fruit development and storage. The development of environmentally-friendly biological control agents has attracted increasing research interest. In this study, we characterized a fungal strain ( LQ) that strongly inhibits .

Advances and prospects of ergothioneine in the treatment of cognitive frailty.

Background: To review the biological functions of ergothioneine (ERGO), its correlation with plasma levels in cognitive frailty, and research progress in treating frailty and cognitive impairment, with the aim of providing a reference for ERGO application in cognitive frailty treatment.

Methods: A comprehensive review of existing literature on ERGO's chemical structure, sources, antioxidant and anti-inflammatory effects, and its role in cognitive frailty was conducted. Clinical trial data and metabolomic studies were also analyzed to understand ERGO's therapeutic potential.

NIb Interaction Sites AUU and GGCU Are Crucial to the Replication of Wheat Yellow Mosaic Virus RNA2.

Wheat yellow mosaic virus (WYMV) is the main cause of wheat yellow mosaic disease. Although its regulation of protein translation and interactions with host proteins are well-studied, independent regulation of the virus genome is poorly understood. This study performed in vitro experiments investigating replication regulation by the 5' UTR and 3' UTR of WYMV RNA2.

Multiview Deep Learning Framework for Precise Prediction of Transcription Factor Binding Sites.

Transcription factors (TFs) are essential proteins that regulate gene expression by specifically binding to transcription factor binding sites (TFBSs) within DNA sequences. Their ability to precisely control the transcription process is crucial for understanding gene regulatory networks, uncovering disease mechanisms, and designing synthetic biology tools. Accurate TFBS prediction, therefore, holds significant importance in advancing these areas of research.

Stable SIFSIX-3-Ni NanoMOF Enabled Green Capture and Circular Utilization of CO/CF/CF: A Carbon-Neutral Strategy for Aluminum Electrolysis Emission Control.

The aluminum electrolysis industry generates massive greenhouse gas emissions dominated by CO and perfluorocarbons (PFCs, CF/CF), presenting dual challenges of climate impact and resource waste. Here, we report a robust nickel-based metal-organic framework (SIFSIX-3-Ni) featuring confined square channels (3.55 Å) that achieves the molecular-sieving separation of CO from CF/CF mixtures.

Chinese Aid for Transportation Infrastructure and Child Health in Africa.

This study investigates the impact of transportation infrastructure financed by Chinese aid on child health in 11 sub-Saharan African countries using Demographic and Health Survey data matched with the precise geospatial features of transportation infrastructure. We find that an additional year of exposure to transportation infrastructure significantly increases children's height-for-age z-scores by 0.041 standard deviations and reduces the likelihood of stunting by 1.

Global Consensus Statement on the Management of Pregnancy in Inflammatory Bowel Disease.

Background & Aims: Pregnancy can be a complex and risk-filled event for women with inflammatory bowel disease (IBD). High-quality studies in this population are lacking, with limited data on medications approved to treat IBD during pregnancy. For patients, limited knowledge surrounding pregnancy impacts pregnancy rates, medication adherence, and outcomes.

The Use of Deep Learning in Primary Agricultural Products Freshness Assessment: A Systematic Review.

Primary agricultural products are closely related to our daily lives, as they serve not only as raw materials for food processing but also as products directly purchased by consumers. These products face the issue of freshness decline and spoilage during both production and consumption. Freshness degradation induces sensory deterioration and nutritional loss and promotes harmful substance accumulation, causing gastrointestinal issues or even endangering life.

Effect of warm water soaking for raw rough rice on in vitro starch digestibility of cooked rice.

Background: The present study investigated the influence of mild heat treatment on harvested raw rough rice (Oryza sativa L. cv. Toyomeki) by warm water soaking at 65 °C with respect to the physicochemical properties and starch digestibility of cooked rice.

Expression of host-specific Lysyloxidase double-stranded RNA in Metarhizhium anisopliae enhanced virulence against the rice pest Sogatella furcifera.

Background: Entomopathogenic fungi show great potential as biological control agents for managing insect pests. However, host defenses have limited the effectiveness of these fungi in practice. Utilizing genetic engineering-based technology could be a promising strategy to enhance the killing efficiency of these fungi against insect pests.

The Impact of Adjuvant Drug Therapy on Overall Survival in Patients with Clear Cell Renal Cell Carcinoma: A Systematic Review and Meta-Analysis.

Purpose Clear cell renal cell carcinoma (ccRCC), the dominant subtype of renal malignancy, has a rising global incidence and mortality. While surgery is the standard of care for localized cases, adjuvant therapy aims to improve outcomes in high-risk postoperative patients. To quantify the clinical value of adjuvant pharmacotherapy, this systematic review and meta-analysis assesses its effect on overall survival (OS), disease-free survival (DFS), and progression-free survival (PFS) in patients with ccRCC.

Screening of Relevant Genes and Signalling Pathways Affecting Adult Urosepsis: A Bioinformatic Analysis.

Purpose: Urosepsis, a condition caused by a urinary tract infection spreading to the bloodstream, has a complex epigenetic behavior in its cellular and molecular pathophysiology. The objective of this study was to identify relevant genes and signaling pathways in adult urosepsis through a bioinformatic analysis of differentially expressed genes (DEGs).

Materials And Methods: In this in-silico study, the GSE69528 dataset, containing 138 total RNA blood samples from patients with sepsis and uninfected controls, was obtained from the Gene Expression Omnibus (GEO) database.

Preliminary Functional Outcome Following Robotic Intracorporeal Orthotopic Ileal Neobladder with Integrated Pelvic Fascial Structure-Sparing in Males with Bladder Cancer.

Purpose: To describe our integrated pelvic fascial structure-sparing (IPFSS) technique for robotic-assisted radical cystectomy (RARC) with intracorporeal orthotopic neobladder (ONB) reconstruction and to evaluate its impact on urinary continence and sexual function in male patients.

Methods: This retrospective observational study was conducted at a single high-volume center. Male bladder cancer patients who underwent IPFSS RARC with ONB were included.