Discovery and Characterization of a Single-Component Halogenase for Phenazine Halogenation.

Halogenated phenazines hold promise as antimicrobial and antibiofilm agents, yet are mainly accessed via chemical synthesis. Herein, we report PezW, a novel single-component flavin-dependent halogenase (FDH) that halogenates phenazine scaffolds, notably enabling enzymatic synthesis of bioactive 2-bromo-1-hydroxyphenazine () and 2,4-bromo-1-hydroxyphenazine (). Structural modeling and mutagenesis revealed key residues critical for substrate binding and catalysis.

Fluorogenic response of thiamine triggered by Cu for sensitive and economical determination of alkaline phosphatase.

Although fluorescent probes have been used extensively for alkaline phosphatase (ALP) sensing, most involve a tedious, prolonged, or high-cost synthesis procedure. Thus, it is still necessary to develop convenient and economical fluorescent probes for sensitive determination of ALP. Herein, a facile fluorescent strategy was designed for ALP detection utilizing the fluorogenic response of thiamine (TH) triggered by Cu (Cu-TH system).

Observation and Control of Chiral Spin Frustration in BiYIG Thin Films.

Chiral interactions within magnetic layers stabilize the formation of noncollinear spin textures, which can be leveraged to design devices with tailored magnetization dynamics. Here, we introduce chiral spin frustration in which energetically degenerate magnetic states frustrate the Dzyaloshinskii-Moriya interaction. We demonstrate magnon-driven switching of the chirally frustrated spin states in Bi-substituted yttrium iron garnet thin films.

Inhibitory effects of Δ8-tetrahydrocannabinol on major hepatic cytochrome P450 enzymes and implications for drug disposition.

The increased use of cannabis in many parts of the United States and other countries has led to a need for a more comprehensive understanding of cannabis constituents and their potential for drug-drug interactions. Δ8-Tetrahydrocannabinol (Δ8-THC) is a psychoactive cannabinoid that is found at low concentrations in cannabis but is growing in popularity, especially where the use of Δ9-THC is restricted. Although certain cannabinoids including cannabidiol (CBD) are known to inhibit several metabolizing enzymes including many in the cytochrome P450 family, the effects of Δ8-THC remain poorly characterized.

The effects of acute carbohydrate supplementation on inflammatory cytokines in individuals performing different exercise intensities: a systematic review and meta-analysis.

This study aims to conduct a systematic review and meta-analysis to examine the effects of carbohydrate supplementation on inflammatory cytokines under different exercise intensities, and to explore the interaction between carbohydrate types and exercise intensity in the regulation of inflammation. A systematic search of PubMed, EBSCOhost, Cochrane, Web of Science and CNKI. Twenty eligible studies were included, reporting on key inflammatory cytokines: IL-6, TNF-α, IL-10, IL-1β, and IL-1ra.

Addressing Challenges in Fluid Flow-Induced Cell Membrane Oscillation by Nanopipettes.

The mechanical properties of cell membranes are crucial to regulating cell morphology and behavior. Nanopipette-sensing has become distinctively appealing for the measurement of cell membrane mechanical properties due to its label-free operation and precise targeting. Recent progress has involved precise control of the movement and fluid flow of the nanopipet, which can induce cell membrane oscillation.

Ultrasound-activated piezoelectric hydrogel scaffold for synergistic immunomodulation and angiogenesis in accelerated wound healing.

Current wound therapies struggle to dynamically regulate immune responses and angiogenesis, often resulting in impaired healing, scarring, and poor tissue regeneration. The development of smart hydrogel scaffolds offers an opportunity to precisely modulate the wound healing process. Here, we present a pioneering wireless immunomodulatory strategy by integrating amino-modified barium titanate (BTN) nanoparticles with a natural collagen matrix, using oxidized gellan gum (OG) as a crosslinker, to fabricate a tilapia collagen (Col)-based biomimetic piezoelectric hydrogel scaffold (Col/OG/BTN).

Structural and functional brain abnormal alteration in patients with type 2 diabetes mellitus: A coordinate-based meta-analysis.

Background: Type 2 diabetes mellitus (T2DM) is a prevalent chronic condition associated with a range of abnormalities in both the functional and structural aspects of the brain. However, existing studies have produced inconsistent results due to disease heterogeneity and small sample size. Therefore, we aim to examine common functional and structural alterations in patients with T2DM.

Explore the changes of metabolites in feces and serum of acute pancreatitis patients with different etiologies by LC-MS based metabolomics strategy.

Background: Acute pancreatitis (AP) is a common abdominal inflammatory disease, which is mainly caused by gallstones, hyperlipidemia, and so on. Previous studies have explored the changes of serum metabolites in patients with AP. However, whether different etiologies have distinct impacts on the fecal and serum metabolites of the AP patients is still in suspense.

A systematic review and coordinate-based meta-analysis of resting-state fMRI in athletes from open and closed skills sports.

The impact of prolonged sports training on athletes' brain functional activity remains inconclusive. A systematic review and coordinate-based meta-analysis of resting-state functional magnetic resonance imaging (rs-MRI) is necessary to identify functional connectivity changes induced by prolonged sports training. A total of 31 studies were included in the systematic review, and 18 studies with 347 non-athletes and 327 athletes were analyzed using coordinate-based activation likelihood estimation (ALE) and seed-based d mapping with permutation of subject images (SDM-PSI) meta-analysis.

Identification of human hepatic UDP-glucuronosyltransferases involved in the glucuronidation of temazepam.

Temazepam is a commonly used 1,4-benzodiazepine that exists as a racemic mixture of R and S enantiomers that are metabolized primarily by glucuronidation via the UDP-glucuronosyltransferases (UGT) enzymes. The goal of the present study was to identify the individual UGTs responsible for catalyzing temazepam enantiomer glucuronidation. There was a 3.

Plasmonic Nanoparticle Monolayer-Decorated Nanopipette Enabling in Situ Monitoring of Single-Cell Redox Metabolism.

The in-depth study of how single cells respond to redox perturbations is pivotal for predicting cell fate decisions. Surface-enhanced Raman spectroscopy (SERS)-active nanopipettes hold promise for single-cell analysis, yet challenges remain in achieving both signal sensitivity and reproducibility. Here, a plasmonic nanoparticle monolayer-decorated nanopipette has been developed, which serves as a biomimetic endoscope to monitor single-cell redox metabolism.

Abnormal Static and Dynamic Functional Connectivity in Tension-Type Headache: A Support Vector Machine Analysis.

Tension-type headache (TTH) is a primary headache with the highest prevalence. Previous studies have revealed the local brain abnormalities of TTH patients. However, little is known about its brain connectivity disruption.

Disposal of municipal solid waste incineration fly ash through synergistic in-plant dechlorination and sintering.

Incineration technology has emerged as the predominant approach for the safe disposal of municipal solid waste in China, generating a significant amount of fly ash as a byproduct. However, the primary landfill method currently employed in China, chelation stabilization coupling, falls short of reliability in terms of environmental standards. Consequently, the safe and effective disposal of fly ash containing high concentrations of chlorine salts, heavy metals, and dioxins is of critical importance.

The characteristic of conventional and extended high-frequency audiometry in older adults - a community-based investigation in South China.

Objectives: To define extended high-frequency hearing threshold ranges in normal hearing elderly (aged 55 years and above) and identify associated risk factors for extended high-frequency hearing loss.

Methods: Pure-tone thresholds (0.25-16 kHz) and word recognition scores were measured using portable audiometric equipment.

Overexpression of CX3CL1-CX3CR1 Signaling in the Stria Vascularis rather than the Basilar Membrane May Be the Trigger for Inflammaging in the Cochlea.

Introduction: Inflammaging is a key mechanism in presbycusis. CX3CL1-CX3CR1 pathway is critical for cochlear macrophage-hair cell cross-talk. However, its role in inflammaging remains unclear.

Preparation and adsorption properties of microsphere geopolymers derived from calcium carbide slag and fly ash.

In this work, using fly ash and calcium carbide slag as mixed precursors, hydrogen peroxide and sodium dodecyl sulfate as mixed foaming agents, porous microsphere geopolymer was prepared by suspension curing technology to remove Cu (II) from aqueous solution. Because the Ca (OH) in calcium carbide slag effectively improves the pozzolana activity of fly ash, more C-S-H gel is produced in the geopolymerization process, and the foaming process makes the material have larger specific surface area and better adsorption properties. The results show that the specific surface area of the material is 63.

Altered resting-state functional connectivity of amygdala subregions in adults with subthreshold depression after aerobic exercise.

Aerobic exercise has been proved to reduce the risk of major depression in subthreshold depression (StD) individuals effectively, yet previous studies ignored the different functions of amygdala subregions. In this study, 44 StD individuals and 34 healthy controls (HCs) underwent resting-state functional magnetic resonance imaging before and after eight weeks of aerobic exercise respectively. Transverse and longitudinal analyses were conducted based on the seed-based functional connectivity (FC) analysis between amygdala subregions and the whole brain of the two groups.

Octreotide attenuates experimental severe acute pancreatitis through inhibiting pyroptosis and modulating intestinal homeostasis.

Severe acute pancreatitis (SAP) is a common clinical condition characterized by acute abdominal symptoms. Octreotide (OCT) is a commonly prescribed treatment for acute pancreatitis (AP). Recent research shows that pyroptosis and intestinal homeostasis significantly contribute to the progression of AP.

Altered brain functional connectivity in patients with tension-type headache.

Objective: To evaluate whether patients with tension-type headache (TTH) exhibit abnormal brain functional connectivity compared to healthy controls.

Background: TTH is one of the most prevalent headache disorders throughout the world. The present study delves into brain functional connectivity in patients with TTH to enhance the understanding of its underlying pathophysiology.

Profile of intestinal fungal microbiota in acute pancreatitis patients and healthy individuals.

Objective: The gut is involved in the development of acute pancreatitis (AP). Increased focus is being given to the role of gut microbiota in the pathogenesis of AP. Nevertheless, there is currently no available evidence regarding the composition of fungal microorganisms in the intestines of patients with AP.

Systemic inflammatory index as a predictive marker for the severity of coronary artery disease in individuals with chronic kidney disease.

Objective: To evaluate the correlation between the inflammatory intensity, as indicated by the systemic inflammatory index (SII), and the severity of coronary artery disease (CAD) in patients with chronic kidney disease (CKD).

Methods: A total of 280 CKD patients who underwent coronary angiography were enrolled. CAD was evaluated using the Gensini score (GS).

Frequency-Specific Alternations in the Amplitude of Fluctuations in Tension-Type Headache: A Machine Learning Study.

Brain neural signal at different frequency bands relates to different functions. However, the frequency-specific properties of spontaneous brain activity in tension-type headache (TTH)-the most rampant primary headache-remain largely unknown. We investigated the local neural activity of 33 TTH patients and 31 healthy controls (HCs) in the conventional frequency band and two sub-frequency bands (slow-4 and slow-5 frequency band), employing fractional amplitude of low-frequency fluctuations (fALFF), percent amplitude fluctuations (PerAF) and Wavelet-ALFF analytic methods.

Experimental Manipulation of the Bilateral Posterior Parietal Cortex Strengthens Associative Memory in Healthy Participants: A Continuous Theta-burst Stimulation.

To test whether targeting left and right posterior parietal cortex (PPC) with continuous theta-burst stimulation (cTBS) in healthy adults would strengthen associative memory (AM) performance. This study consisted of two experiments (a behavioral experiment and a formal experiment during each of the two experimental sessions). In Experiment 1, 18 adults (one male, ages = 22.