NIb Interaction Sites AUU and GGCU Are Crucial to the Replication of Wheat Yellow Mosaic Virus RNA2.

Wheat yellow mosaic virus (WYMV) is the main cause of wheat yellow mosaic disease. Although its regulation of protein translation and interactions with host proteins are well-studied, independent regulation of the virus genome is poorly understood. This study performed in vitro experiments investigating replication regulation by the 5' UTR and 3' UTR of WYMV RNA2.

Electroclinical features and surgical outcome of cingulate epilepsy.

Background: Cingulate epilepsy is rare and can manifest with variable semiology features. The symptomatic diversity elucidates ictal involvement of certain subregions of the cingulate gyrus and early spread patterns. Knowledge of the features of cingulate epilepsy is important for better localization and surgical strategy.

Design, synthesis, structure-activity relationship (SAR) and analgesic effect studies of novel arylsulfonamides as selective Nav1.7 inhibitors.

Chronic pain has become a major factor affecting the quality of human life. Nav1.7 is a subtype of neuronal voltage-gated sodium channel.

Reconstruction of structured illumination microscopy for live imaging in low light with lightweight neural networks.

Structured illumination microscopy (SIM) as a type of super-resolution optical microscopy technique has been widely used in the fields of biophysics, neuroscience, and cell biology research. However, this technique often requires high-intensity illumination and multiple image acquisitions to generate a single high-resolution image. This process not only significantly reduces the imaging speed, but also increases the exposure time of samples to intense light, leading to increased phototoxicity and photobleaching issues, especially prominent in live cell imaging.

Design and Discovery of Novel Selective RET Inhibitors with High Central Nervous System Penetration, Enhanced Pharmacokinetics, and Pharmacodynamics.

RET (rearrangement during transfection) is a transmembrane glycoprotein receptor tyrosine kinase. In pathological conditions, the RET mutation, gene fusion, and overexpression result in aberrant RET signal transduction in tumor cells, which activates downstream pathways and promotes tumor growth and metastasis. Selective inhibition of RET kinase could effectively treat RET-driven cancers.

A coupled system accelerating the photocatalytic reduction of captured CO to methane.

Herein, we develop a flow-coupled system using a bifunctional Pd/TiO-TEPA/SiO composite that integrates CO capture and photocatalytic reduction. The amine-functionalized SiO pores selectively enrich diluted CO (10%) near Pd/TiO active sites, creating a CO-rich microenvironment that promotes CO adsorption and lowers the energy barrier for *COOH intermediate formation. This system boosts the conversion of diluted CO into CH with a production rate of up to 5.

Pre-ictal causal connectivity reveals the epileptic network characteristics for deep brain stimulation.

Deep brain stimulation of anterior nucleus of the thalamus (ANT-DBS) is an effective clinical treatment for drug-resistant focal epilepsy. However, the complex epileptic network characteristics underlying ANT-stimulation effectiveness remain unknown, owing to currently unclear connectivity between ANT and seizure-related cortex in pre-ictal periods. Here, we developed a novel individualized pre-ictal ANT-cortical tripartite connectivity network (PANT-CTCNet), aiming to reveal epileptic network characteristics using intracranial stereo-electroencephalography (sEEG) recordings in five patients with focal epilepsy for 90 trials.

Carrier-free Nanotherapeutics unleashed: Ce6/siPD-L1 co-delivery system synergizes photodynamic and RNAi therapies to combat breast cancer.

Small interfering RNA targeting PD-L1 (siPD-L1) demonstrates therapeutic potential in cancer immunotherapy, but faces challenges including inefficient lysosomal escape and carrier-induced toxicity. To address these limitations, we developed a carrier-free nanoparticle (NP) system, TPP-Ce6@siPD-L1. The amino-modified triphenylphosphine (TPP) was covalently connected with Ce6 to form an amphiphilic complex, which then loaded siPD-L1 creating an efficient co-delivery system for photosensitizers (PS) and gene drugs.

Design and Synthesis of Novel 5,6,7,8-Tetrahydropyrido[2,3-]pyrimidine Derivatives as VCP/p97 Inhibitors for the Treatment of Acute Myeloid Leukemia (AML).

Background: VCP/p97 plays an important role in endoplasmic reticulum related degradation pathways, and inhibition of p97 was shown to induce ER stress and subsequently cell death in a variety of solid tumors and hematoma. For acute myeloid leukemia (AML) cells, inhibition of p97 activity leads to the accumulation of ubiquitylated proteins, activation of unfolded protein response (UPR) and apoptosis.

Methods: We have designed and synthesized a series of novel 5,6,7,8-tetrahydropyridine[2,3-d]pyrimidine derivatives.

Expansion Force-Based Adaptive Multistage Constant Current Fast Charging with Lithium Plating Detection for Lithium-Ion Batteries.

The multistage constant current (MCC) charging protocol for lithium-ion batteries is commonly used to balance lithium plating and charging time. Traditional methods depend on a pre-defined charging map without considering the feedback of lithium plating and subsequent self-regulation of the charging rate. To tackle this problem, an adaptive MCC charging method is proposed, which is based on expansion force feedback to detect lithium plating.

Symptomatic Emotional Responses and Changes in Networks Elicited by Direct Electrical Stimulation.

Aim: Emotion is a major area of research in psychology and neuroscience. However, the role of direct electrical stimulation (DES) in emotional localization has not yet been fully explored. This study aimed to analyze the use of DES in examining the local connectivity of brain regions eliciting emotional responses, thereby providing evidence for a new perspective of local changes in brain networks during emotional responses.

Structural network-specific effect of extreme capsule stimulation for drug-resistant focal epilepsy.

Treatment for drug-resistant epilepsy in poor candidates for resection surgeries remains challenging. The prevailing deep brain stimulation of subcortical nuclei is effective but exhibits heterogeneous efficacy and unpredictable side effects. Therefore, the investigation of novel deep brain stimulation targets is of paramount importance.

Emerging translocator protein-positron emission tomographic imaging improves detection of focal cortical dysplasia.

Objective: The identification of epileptic lesions is crucial for improving surgical outcomes. Nevertheless, substantial focal cortical dysplasia (FCD) may be invisible on magnetic resonance imaging (MRI). We aimed to characterize the expression pattern of 18-kDa translocator protein (TSPO) in FCD and to evaluate the effectiveness of this inflammation-reflective molecular imaging technique for detecting FCD.

Investigating the inhibition of benzimidazole derivatives on SARS-CoV-2 M by enzyme activity inhibition, spectroscopy, and molecular docking.

The inhibition of twenty-five 1,2-fused/disubstituted benzimidazoles on the SARS-CoV-2 M were investigated in this work. It was found that four compounds (, , and ) showed obvious inhibitory effect on M. The inhibitory effect of (IC 46.

Buffered Hydroxyl Radical for Photocatalytic Non-Oxidative Methane Coupling.

Hydroxy radical (⋅OH) is a prestigious oxidant that allows the cleavage of strong chemical bonds of methane but is untamed, leading to over-oxidation of methane and waste of oxidants, especially at high methane conversion. Here, we managed to buffer ⋅OH in an aqueous solution of photo-irradiated Fe, where ⋅OH almost participates in methane oxidation. Due to the interaction between Fe and SO , the electron transfer from OH to excited-state Fe for ⋅OH generation is retarded, while excessive ⋅OH is consumed by generated Fe to restore Fe.

Porphyromonas gingivalis inducing autophagy-related biological dysfunction in alveolar epithelial cells: an in vitro study.

Background: Chronic obstructive pulmonary disease (COPD) is a respiratory disease with high morbidity and mortality. Notably, the pathogenesis and progression of COPD are related to lung infection, inflammatory response, and biological dysfunction in alveolar epithelial cells. Studies also found that periodontitis is an independent risk factor for COPD.

Porphyromonas gingivalis-induced autophagy exacerbates abnormal lung homeostasis: An in vivo and in vitro study.

Objective: The aim of this study was to evaluate the effect of periodontal Porphyromonas gingivalis (P. gingivalis) infection on lung homeostasis and to explore the underlying mechanism.

Designs: In in vivo experiments, twelve mice were divided into two groups.

Urban Lake Health Assessment Based on the Synergistic Perspective of Water Environment and Social Service Functions.

Urban lakes serve as vital ecological and recreational anchors within built environments, essential for enhancing urban resilience. Evaluating lake health predominantly focuses on water quality, assessing indicators such as nutrient levels, toxicity, pH balance, and water clarity to monitor changes. This study proposes a comprehensive evaluation framework that systematically describes specific spatiotemporal manifestations and periodic exogenous regulation characteristics across five dimensions: physical structure, water quality, shoreline dynamics, external regulation, and social service.

Glucose oxidase and MnO functionalized liposome for catalytic radiosensitization enhanced synergistic breast cancer therapy.

In recent years, the integration of radiotherapy and nanocatalytic medicine has gained widespread attention in the treatment of breast cancer. Herein, the glucose oxidase (GOx) and MnO nanoparticles co-modified multifunctional liposome of GOx-MnO@Lip was constructed for enhanced radiotherapy. Introduction of GOx would not only elevate the glucose consumption to starve the cancer cells, but also increased the endogenous HO level.

Predicting neurodevelopmental outcomes in extremely preterm neonates with low-grade germinal matrix-intraventricular hemorrhage using synthetic MRI.

Objectives: This study aims to assess the predictive capability of synthetic MRI in assessing neurodevelopmental outcomes for extremely preterm neonates with low-grade Germinal Matrix-Intraventricular Hemorrhage (GMH-IVH). The study also investigates the potential enhancement of predictive performance by combining relaxation times from different brain regions.

Materials And Methods: In this prospective study, 80 extremely preterm neonates with GMH-IVH underwent synthetic MRI around 38 weeks, between January 2020 and June 2022.

Oral infection with periodontal pathogens induced chronic obstructive pulmonary disease-like lung changes in mice.

Background: Epidemiological studies have demonstrated that periodontitis is an independent risk factor for chronic obstructive pulmonary disease (COPD). However, the mechanism underlying the association between these two diseases remains unclear. The lung microbiota shares similarities with the oral microbiota, and there is growing evidence to suggest that the lung microbiome could play a role in the pathogenesis of COPD.

The value of synthetic MRI in detecting the brain changes and hearing impairment of children with sensorineural hearing loss.

Introduction: Sensorineural hearing loss (SNHL) can arise from a diverse range of congenital and acquired factors. Detecting it early is pivotal for nurturing speech, language, and cognitive development in children with SNHL. In our study, we utilized synthetic magnetic resonance imaging (SyMRI) to assess alterations in both gray and white matter within the brains of children affected by SNHL.

Evaluate climate change and anthropogenic activities influencing geochemical variations in sediment between and within the avulsion period in the Lower Yellow River avulsion channels.

The geochemical data from sediments in avulsion channels provide historical evidence of climate change and human-induced alterations in river basin environments. The present study focused on the particle size of sediments in cores and the level of geochemical variation in avulsion channels of the Lower Yellow River Delta (YRD), China. The sediment samples were collected in a depth range of 20-400 cm in avulsion channels.